JPH11103788A - Feed for domestic animal - Google Patents
Feed for domestic animalInfo
- Publication number
- JPH11103788A JPH11103788A JP9281162A JP28116297A JPH11103788A JP H11103788 A JPH11103788 A JP H11103788A JP 9281162 A JP9281162 A JP 9281162A JP 28116297 A JP28116297 A JP 28116297A JP H11103788 A JPH11103788 A JP H11103788A
- Authority
- JP
- Japan
- Prior art keywords
- milk
- basic protein
- protein composition
- feed
- fetal growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001465754 Metazoa Species 0.000 title abstract description 11
- 239000000203 mixture Substances 0.000 claims abstract description 40
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 claims abstract description 35
- 235000013336 milk Nutrition 0.000 claims abstract description 18
- 210000004080 milk Anatomy 0.000 claims abstract description 18
- 239000008267 milk Substances 0.000 claims abstract description 18
- 150000001413 amino acids Chemical class 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 11
- 239000007952 growth promoter Substances 0.000 claims abstract description 6
- 239000004480 active ingredient Substances 0.000 claims abstract description 5
- 239000000470 constituent Substances 0.000 claims abstract description 5
- 230000004578 fetal growth Effects 0.000 claims description 31
- 230000001737 promoting effect Effects 0.000 claims description 26
- 244000144972 livestock Species 0.000 claims description 21
- 210000003754 fetus Anatomy 0.000 abstract description 8
- 239000003729 cation exchange resin Substances 0.000 abstract description 4
- 235000020247 cow milk Nutrition 0.000 abstract description 3
- 239000011347 resin Substances 0.000 abstract description 3
- 229920005989 resin Polymers 0.000 abstract description 3
- 230000012010 growth Effects 0.000 abstract description 2
- 239000012528 membrane Substances 0.000 abstract description 2
- 238000000108 ultra-filtration Methods 0.000 abstract description 2
- 239000007858 starting material Substances 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 9
- 239000003795 chemical substances by application Substances 0.000 description 7
- 235000001014 amino acid Nutrition 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 208000036364 Normal newborn Diseases 0.000 description 4
- 206010036590 Premature baby Diseases 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 241000700159 Rattus Species 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 231100000915 pathological change Toxicity 0.000 description 3
- 230000036285 pathological change Effects 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 208000006386 Bone Resorption Diseases 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000024279 bone resorption Effects 0.000 description 2
- 230000035606 childbirth Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 235000020251 goat milk Nutrition 0.000 description 2
- 235000020256 human milk Nutrition 0.000 description 2
- 210000004251 human milk Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000008935 nutritious Nutrition 0.000 description 2
- 210000000963 osteoblast Anatomy 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 235000020254 sheep milk Nutrition 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 239000004470 DL Methionine Substances 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 102000010445 Lactoferrin Human genes 0.000 description 1
- 108010063045 Lactoferrin Proteins 0.000 description 1
- 108010023244 Lactoperoxidase Proteins 0.000 description 1
- 102000045576 Lactoperoxidases Human genes 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 241000772415 Neovison vison Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 208000005107 Premature Birth Diseases 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- XAAHAAMILDNBPS-UHFFFAOYSA-L calcium hydrogenphosphate dihydrate Chemical compound O.O.[Ca+2].OP([O-])([O-])=O XAAHAAMILDNBPS-UHFFFAOYSA-L 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 235000019700 dicalcium phosphate Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- CSSYQJWUGATIHM-IKGCZBKSSA-N l-phenylalanyl-l-lysyl-l-cysteinyl-l-arginyl-l-arginyl-l-tryptophyl-l-glutaminyl-l-tryptophyl-l-arginyl-l-methionyl-l-lysyl-l-lysyl-l-leucylglycyl-l-alanyl-l-prolyl-l-seryl-l-isoleucyl-l-threonyl-l-cysteinyl-l-valyl-l-arginyl-l-arginyl-l-alanyl-l-phenylal Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 CSSYQJWUGATIHM-IKGCZBKSSA-N 0.000 description 1
- 235000021242 lactoferrin Nutrition 0.000 description 1
- 229940078795 lactoferrin Drugs 0.000 description 1
- 229940057428 lactoperoxidase Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 1
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- FFEARJCKVFRZRR-UHFFFAOYSA-N methionine Chemical compound CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 235000006109 methionine Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000002997 osteoclast Anatomy 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- PJAHUDTUZRZBKM-UHFFFAOYSA-K potassium citrate monohydrate Chemical compound O.[K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O PJAHUDTUZRZBKM-UHFFFAOYSA-K 0.000 description 1
- 229940050931 potassium citrate monohydrate Drugs 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、妊娠母獣に投与
し、胎仔の発育を促進させることを目的とした胎仔発育
促進剤及び胎仔発育促進効果を賦与した家畜飼料に関す
る。本発明の胎仔発育促進剤又は胎仔発育促進効果を賦
与した家畜飼料は、妊娠母獣中の胎仔の発育を促進させ
ることができるので、家畜を育種する際に極めて有用で
ある。TECHNICAL FIELD The present invention relates to a fetal growth promoting agent which is administered to a pregnant mother to promote fetal growth, and a livestock feed provided with a fetal growth promoting effect. The livestock feed provided with the fetal growth promoting agent or the fetal growth promoting effect of the present invention can promote the growth of a fetus in a pregnant mother, and is therefore extremely useful in breeding livestock.
【０００２】[0002]
【従来の技術】近年、畜産物生産の効率化が求められ、
家畜の繁殖や育種における損失を如何に防止するかが重
要な課題となってきている。家畜を含め動物において
は、生まれてきた新生仔の体重が普通の新生仔の平均体
重よりも軽かった場合、その後の食環境が同じであれ
ば、その体重差が解消されることはないといわれてい
る。普通の新生仔の平均体重よりも軽い新生仔、特に未
熟仔が誕生するという問題は、多胎動物の場合、その出
産仔数にも影響を受ける。一般に、出産仔数が多ければ
多い程、未熟仔出産の可能性は高まる。そして、この未
熟仔は家畜としての経済価値を著しく損なうこともあ
る。また、正常出産であった場合でも１仔当たりの体重
は、普通の新生仔の平均体重より軽い。したがって、こ
のような新生仔の体重を普通の体重まで回復させるため
には、より栄養価の高い飼料を与える必要があり、これ
が生産効率低下の一因となる。2. Description of the Related Art In recent years, there has been a demand for more efficient production of livestock products.
An important issue is how to prevent losses in livestock breeding and breeding. It is said that in animals including livestock, if the weight of the newborn born is lighter than the average weight of a normal newborn, the weight difference will not be eliminated if the subsequent eating environment is the same. ing. The problem of having newborns weighing less than the average weight of normal newborns, especially immature offspring, is also affected by the number of offspring in the case of multiple fetuses. In general, the greater the number of offspring, the greater the likelihood of immature offspring. The immature offspring can significantly impair the economic value of livestock. In addition, even if the birth is normal, the weight per pup is lower than the average weight of a normal newborn. Therefore, in order to restore the weight of such newborn babies to normal weight, it is necessary to provide a more nutritious feed, which contributes to a decrease in production efficiency.
【０００３】現在、家畜育種における未熟仔への対応
は、対症療法的な飼料給与のみに重点が置かれている。
また、未熟仔出産を防止する手段としては、ホルモン投
与による妊娠の維持等、薬物的な処方が主体であるが、
薬物投与を施すには獣医師の処方が必要であり、それに
要する費用も高額なものとなっている。さらに、母獣に
対する飼料給与で未熟仔出産を防止したり、胎仔の発育
を促進するという方法も考えられるが、妊娠期間中に栄
養価の高い飼料を過剰に給与することは、かえって分娩
事故を起こし易いという問題もあり、母獣に影響を与え
ずに胎仔の発育のみを促進させるという方法は見出せな
い現状にある。しかしながら、新生仔の体重が普通の新
生仔の平均体重を上回っているならば、飼料給与におけ
る損失を全く考慮する必要がなく、さらに、栄養価の高
い飼料を給与することにより、家畜としての経済価値を
一層高めることは明らかである。[0003] Currently, the response to immature pups in livestock breeding focuses solely on symptomatic feed feeding.
In addition, as a means of preventing premature birth, drug prescription such as maintenance of pregnancy by hormone administration is mainly used,
Veterinary prescribing is required to administer drugs, and the costs involved are high. In addition, it is conceivable to prevent the delivery of premature babies or to promote the development of the fetus by feeding the mothers with feed.However, excessive feeding of nutrient-rich feed during pregnancy can actually result in childbirth accidents. There is also a problem that it is easy to occur, and at present, there is no way to find a method of promoting only the development of the fetus without affecting the mother animal. However, if the weight of the newborn is above the average weight of a normal newborn, there is no need to consider any losses in feed feeding, and by feeding nutritious feed, the economics of livestock It is clear that the value will be further increased.
【０００４】また、牛乳、人乳、山羊乳、羊乳等、哺乳
類の乳を陽イオン交換体等で処理することにより得ら
れ、主としてラクトフェリンやラクトパーオキシダーゼ
等からなる塩基性タンパク質組成物が知られている。こ
の塩基性タンパク質組成物は、リジン、ヒスチジン、ア
ルギニン等の塩基性アミノ酸を比較的多量に含有してい
るという特徴を有する。そして、この塩基性タンパク質
組成物が、骨芽細胞増殖活性及び破骨細胞骨吸収抑制活
性を示し、経口的投与により骨を強化する作用を有する
ことが知られている (特開平8-151331号公報) 。一方、
本発明者等は、乳又は乳由来の原料を陽イオン交換樹脂
に接触処理して得られる、等電点が 7.5〜11で、構成ア
ミノ酸として塩基性アミノ酸を10重量％以上含有してい
る乳由来の塩基性タンパク質組成物が、骨芽細胞増進促
進、骨強化及び骨吸収作用を有しており、これを飲食
品、医薬又は飼料として利用できることを先に出願 (特
願平8- 28459号) したが、本発明者はさらに乳由来の塩
基性タンパク質組成物が家畜飼料に胎仔発育促進効果を
有することを見出し、本発明を完成するに至った。A basic protein composition obtained by treating mammalian milk, such as cow's milk, human milk, goat's milk, sheep's milk, with a cation exchanger, etc., and mainly comprising lactoferrin, lactoperoxidase, etc. is known. Have been. This basic protein composition is characterized in that it contains a relatively large amount of basic amino acids such as lysine, histidine and arginine. It is known that this basic protein composition exhibits osteoblast proliferation activity and osteoclast bone resorption inhibiting activity, and has an action of strengthening bone by oral administration (Japanese Patent Application Laid-Open No. 8-151331). Gazette). on the other hand,
The present inventors have proposed a milk obtained by contacting milk or a milk-derived raw material with a cation exchange resin, having an isoelectric point of 7.5 to 11, and containing at least 10% by weight of a basic amino acid as a constituent amino acid. Derived basic protein composition has osteoblast enhancement promotion, bone strengthening and bone resorption effects, and it has been previously filed that it can be used as food and drink, medicine or feed (Japanese Patent Application No. 8-28459). However, the present inventors have further found that a milk-derived basic protein composition has an effect of promoting fetal growth in livestock feed, and have completed the present invention.
【０００５】[0005]
【発明が解決しようとする課題】即ち、本発明者等は、
上述したような家畜新生仔の体重に関わる問題に注目
し、妊娠母獣に経口的投与することにより、胎仔の発育
を促進することができるような物質を求めて、鋭意研究
を進めていたところ、上記塩基性タンパク質組成物を妊
娠母獣に経口的投与することで、母体に無理なく胎仔の
発育を選択的に促進することができることを見出し、本
発明を完成するに至った。したがって、本発明は、乳由
来の塩基性タンパク質組成物を有効成分とする胎仔発育
促進剤を提供することを課題とする。また、本発明は、
乳由来の塩基性タンパク質組成物を配合して胎仔発育促
進効果を賦与した家畜飼料を提供することを課題とす
る。なお、本発明でいう家畜とは、牛、馬、豚、山羊、
羊、犬、猫、ミンク等である。That is, the inventors of the present invention have
Focusing on the above-mentioned problems relating to the weight of newborn domestic animals, we have been conducting intensive research in search of substances that can promote fetal development by oral administration to pregnant mothers. By orally administering the above-mentioned basic protein composition to pregnant mothers, they found that the development of a fetus could be selectively promoted without difficulty in the mother and completed the present invention. Therefore, an object of the present invention is to provide a fetal growth promoting agent containing a milk-derived basic protein composition as an active ingredient. Also, the present invention
It is an object of the present invention to provide a livestock feed that has a fetal growth promoting effect by incorporating a milk-derived basic protein composition. In the present invention, livestock refers to cows, horses, pigs, goats,
Sheep, dogs, cats, mink, etc.
【０００６】[0006]
【課題を解決するための手段】本発明では、胎仔発育促
進剤の有効成分として塩基性タンパク質組成物を使用す
るものであり、家畜飼料に胎仔発育促進効果を賦与する
ために塩基性タンパク質組成物を配合する。この塩基性
タンパク質組成物は、上述のように公知のもので、例え
ば乳又は乳由来の原料を陽イオン交換樹脂に接触させた
後、樹脂に吸着した画分を塩濃度 0.1〜2.0molの溶出液
で溶出することにより得られる画分を、(i) 80℃以上で
加熱するか、(ii)最終濃度が10〜50％となるようにアル
コールを添加するか、(iii)最終濃度が0.2mol以上とな
るまで塩類を添加するかのいずれかの方法で生ずる沈澱
を除去し、上清を回収するか、又は前記した塩濃度 0.1
〜2.0molの溶出液で溶出した画分を、分画分子量30,000
以上の限外濾過膜で処理し、透過側の溶液を回収するこ
とにより得られ、乳中に微量にしか存在しない塩基性タ
ンパク質画分を分画したもので、ＳＤＳ−ポリアクリル
アミド電気泳動による分子量分布は、 2,000〜24,000ダ
ルトンであり、等電点は 7.5〜11である乳由来の塩基性
タンパク質組成物であって、構成アミノ酸として、好ま
しくは塩基性アミノ酸を10重量％以上含有するものであ
る。なお、本発明の上記塩基性タンパク質組成物を得る
ための乳としては、牛乳、人乳、山羊乳、羊乳を挙げる
ことができ、これらの乳をそのまま、あるいは、これら
の乳の還元乳、脱脂乳、ホエー等を使用すれば良い。According to the present invention, a basic protein composition is used as an active ingredient of a fetal growth promoter, and the basic protein composition is used to impart a fetal growth promoting effect to livestock feed. Is blended. This basic protein composition is known as described above, for example, after contacting milk or milk-derived raw material with a cation exchange resin, the fraction adsorbed on the resin is eluted with a salt concentration of 0.1 to 2.0 mol. The fraction obtained by elution with the liquid is heated at (i) 80 ° C. or higher, (ii) alcohol is added so that the final concentration is 10 to 50%, or (iii) the final concentration is 0.2%. The resulting precipitate is removed by any of the methods of adding salts until the concentration reaches 0.1 mol or more, and the supernatant is recovered, or the salt concentration described above is 0.1%.
The fraction eluted with ~ 2.0 mol of the eluate was subjected to a fractionation molecular weight of 30,000
It is obtained by recovering the solution on the permeate side by treating with the above ultrafiltration membrane and fractionating a basic protein fraction which is present only in trace amounts in milk, and has a molecular weight by SDS-polyacrylamide electrophoresis. A milk-derived basic protein composition having a distribution of 2,000 to 24,000 daltons and an isoelectric point of 7.5 to 11, preferably containing at least 10% by weight of a basic amino acid as a constituent amino acid . The milk for obtaining the basic protein composition of the present invention includes cow's milk, human milk, goat's milk, and sheep's milk. Skim milk, whey or the like may be used.
【０００７】[0007]
【発明の実施の形態】本発明の塩基性タンパク質組成物
を有効成分とする胎仔発育促進剤は、粉末や錠剤等の形
態で、直接、妊娠母獣に経口投与する。また、塩基性タ
ンパク質組成物を胎仔発育促進剤として配合し、胎仔発
育促進効果を賦与した家畜飼料を妊娠母獣に経口投与す
る。このように、妊娠母獣に本発明の胎仔発育促進剤や
胎仔発育促進効果を賦与した家畜飼料を経口投与するこ
とにより、胎仔の発育を促進することができる。そし
て、未熟仔出産を防止したり、新生仔の体重増加に寄与
することができる。なお、妊娠母獣に本発明の胎仔発育
促進剤や胎仔発育促進効果を賦与した家畜飼料を投与す
るに際しては、一日当たり、塩基性タンパク質組成物を
0.1g/kg程度摂取できるよう考慮して投与すれば良い。
また、塩基性タンパク質組成物を配合して家畜飼料に胎
仔発育促進効果を賦与するためには、塩基性タンパク質
組成物を 0.1〜1.0 重量％の割合で家畜飼料に配合する
ことが好ましい。BEST MODE FOR CARRYING OUT THE INVENTION The fetal growth promoter containing the basic protein composition of the present invention as an active ingredient is orally administered directly to pregnant mothers in the form of powder or tablets. In addition, a basic protein composition is blended as a fetal growth promoting agent, and a livestock feed having an effect of promoting fetal growth is orally administered to pregnant mothers. Thus, the fetal growth can be promoted by orally administering the fetal growth promoting agent of the present invention or a livestock feed provided with the fetal growth promoting effect to a pregnant mother animal. And it can prevent the delivery of premature babies and contribute to weight gain of newborn babies. In addition, when administering a fetal growth promoting agent of the present invention or livestock feed imparted with a fetal growth promoting effect to a pregnant mother animal, the basic protein composition per day,
It may be administered in consideration of taking about 0.1 g / kg.
Further, in order to impart a fetal growth promoting effect to livestock feed by blending the basic protein composition, it is preferable to blend the basic protein composition in a livestock feed at a ratio of 0.1 to 1.0% by weight.
【０００８】次に、実施例及び試験例を示し、本発明を
さらに詳しく説明する。Next, the present invention will be described in more detail with reference to Examples and Test Examples.
【実施例１】陽イオン交換樹脂（スルホン化キトパー
ル；富士紡績製）を充填したカラムに脱脂乳 50 l を通
液した後、2mol塩化ナトリウム水溶液を通液し、樹脂に
吸着した塩基性タンパク質を溶出して回収した。そし
て、この塩基性タンパク質を含む溶出液をゲル濾過し
て、分子量 2,000〜20,000の画分を回収し、凍結乾燥し
て、塩基性タンパク質組成物 23gを得た。このようにし
て得られた塩基性タンパク質組成物の成分組成を表１に
示す。Example 1 After passing 50 l of skim milk through a column packed with a cation exchange resin (sulfonated chitopearl; manufactured by Fuji Boseki), a 2 mol aqueous solution of sodium chloride was passed through to remove the basic protein adsorbed on the resin. Eluted and collected. Then, the eluate containing the basic protein was subjected to gel filtration to collect a fraction having a molecular weight of 2,000 to 20,000, followed by freeze-drying to obtain 23 g of a basic protein composition. Table 1 shows the component compositions of the basic protein composition thus obtained.
【０００９】[0009]
【表１】 ─────────────── 水分 1.4 (％) タンパク質 95.4 脂肪 0.4 灰分 0.8 その他 2.0 ───────────────[Table 1] 1.4 Moisture 1.4 (%) Protein 95.4 Fat 0.4 Ash content 0.8 Other 2.0 ───────────────
【００１０】[0010]
【試験例１】実施例１で得られた塩基性タンパク質組成
物を妊娠ラットに投与して、胎仔発育促進効果を調べ
た。すなわち、妊娠１週目（プラーク確認後１週目）の
ＳＤ系妊娠ラットを４匹ずつ対照群及び試験群の２群に
分け、出産までの約２週間、対照飼料又は試験飼料を投
与した。各飼料の組成を表２に示す。なお、試験飼料に
ついては１日当たり 30gの制限給餌とし、水は水道水を
自由摂取させた。Test Example 1 The basic protein composition obtained in Example 1 was administered to pregnant rats to examine the effect of promoting fetal growth. That is, the SD pregnant rats at the first week of pregnancy (the first week after plaque confirmation) were divided into two groups of a control group and a test group by four rats, and the control feed or the test feed was administered for about 2 weeks until childbirth. Table 2 shows the composition of each feed. The test feed was restricted to 30 g per day, and tap water was freely available.
【００１１】[0011]
【表２】 ─────────────────────────────────── 対照群 試験群 ─────────────────────────────────── カゼイン 20.0 (％) 19.046 (％) コーンスターチ 15.0 15.0 セルロース 5.0 5.0 コーン油 5.0 5.0 ＤＬ−メチオニン 0.3 0.3 ビタミン混合* 1.0 1.0 ミネラル混合* 0.1 0.1 リン酸水素カルシウム・２水和物 1.3 1.3 硫酸カリウム 0.2 0.2 塩化ナトリウム 0.3 0.3 クエン酸カリウム・１水和物 0.8 0.8 酸化マグネシウム 0.1 0.1 ショ糖 50.9 50.854 塩基性タンパク質組成物 ── 1.0 ─────────────────────────────────── * ：ビタミン混合及びミネラル混合については、AIN-76組成に準拠した。 そして、出産直後の新生仔の体重を測定した。その結果を表３に示す。[Table 2] ─────────────────────────────────── Control group Test group ─────── ──────────────────────────── Casein 20.0 (%) 19.046 (%) Corn starch 15.0 15.0 Cellulose 5.0 5.0 Corn oil 5.0 5.0 DL-methionine 0.3 0.3 Vitamin mixture * 1.0 1.0 Mineral mixture * 0.1 0.1 Calcium hydrogen phosphate dihydrate 1.3 1.3 Potassium sulfate 0.2 0.2 Sodium chloride 0.3 0.3 Potassium citrate monohydrate 0.8 0.8 Magnesium oxide 0.1 0.1 Sucrose 50.9 50.854 Base Protein composition ── 1.0 ─────────────────────────────────── * : About vitamin and mineral mixture Was based on the AIN-76 composition. Then, the weight of the newborn immediately after delivery was measured. Table 3 shows the results.
【００１２】[0012]
【表３】 ──────────────────────────────────── 対照群 試験群 ─────────────────────────── １ ２ ３ ４ １ ２ ３ ４ ──────────────────────────────────── 出産仔数 (匹) 9 10 16 10 16 15 11 9 平均体重 (g) 6.95 7.03 6.19 7.02 6.97 6.23 7.41 7.50 標準偏差値 0.73 0.26 0.38 0.50 0.53 0.43 0.34 0.84 ──────────────────────────────────── この試験では、両群の出産仔数に大きな差が生じたた
め、共分散分析により評価した。その結果、試験群の新
生仔体重は、対照群の新生仔体重より有意に上昇してお
り、塩基性タンパク質組成物が胎仔発育促進効果を発揮
することが明らかとなった。なお、試験期間中において
は、母獣に何ら病態的な変化は認められなかった。[Table 3] ──────────────────────────────────── Control group Test group ────── {1 2 3 4 1 2 3 4}数 Number of offspring (animals) 9 10 16 10 16 15 11 9 Average weight (g) 6.95 7.03 6.19 7.02 6.97 6.23 7.41 7.50 Standard deviation 0.73 0.26 0.38 0.50 0.53 0.43 0.34 0.84 に In this study, the number of offspring Since a large difference occurred, evaluation was made by covariance analysis. As a result, the weight of the newborn in the test group was significantly higher than the weight of the newborn in the control group, and it was revealed that the basic protein composition exerts the effect of promoting fetal growth. During the test period, no pathological changes were observed in the dams.
【００１３】[0013]
【実施例２】実施例１で得られた塩基性タンパク質組成
物を配合して、表４に示したような胎仔発育促進効果を
賦与した多胎動物である豚用飼料を製造した。Example 2 The basic protein composition obtained in Example 1 was blended to produce a feed for swine, a multi-fetal animal having a fetal growth promoting effect as shown in Table 4.
【００１４】[0014]
【表４】 ─────────────────────── 大豆粕 7.0 (g/100g) 魚粉 2.0 大麦 35.9 ビタミン類及びミネラル類の 二種混合 40.0 脱脂米糠 3.0 フスマ 8.5 ルーサンミール 3.0 食塩 0.5 塩基性タンパク質組成物 0.1 ───────────────────────[Table 4] ─────────────────────── Soybean meal 7.0 (g / 100g) Fish meal 2.0 Barley 35.9 Two kinds of vitamins and minerals 40.0 Degreasing Rice bran 3.0 bran 8.5 rusan meal 3.0 salt 0.5 basic protein composition 0.1 ───────────────────────
【００１５】この飼料を妊娠した豚に投与して、胎仔発
育促進効果を調べたところ、新生仔体重は平均的な新生
仔体重よりも上昇していた。なお、母獣には何ら病態的
な変化は認められなかった。When this feed was administered to pregnant pigs and the effect of promoting fetal growth was examined, the weight of the newborn was higher than the average weight of the newborn. No pathological changes were observed in the dams.
【００１６】[0016]
【実施例３】実施例１で得られた塩基性タンパク質組成
物を配合して、表５に示したような胎仔発育促進効果を
賦与した単胎動物である牛用飼料を製造した。Example 3 The basic protein composition obtained in Example 1 was blended to produce a single animal feed for cattle, which was given a fetal growth promoting effect as shown in Table 5.
【００１７】[0017]
【表５】 ─────────────────────── 大豆粕 10.0 (g/100g) 大麦 50.9 脱脂米糠 10.0 フスマ 27.0 食塩 1.0 カルシウム 1.0 塩基性タンパク質組成物 0.1 ───────────────────────[Table 5] ─────────────────────── Soybean meal 10.0 (g / 100g) Barley 50.9 Defatted rice bran 10.0 Fuma 27.0 Salt 1.0 Calcium 1.0 Basic protein composition Object 0.1 0.1
【００１８】この飼料を妊娠した牛に投与して、胎仔発
育促進効果を調べたところ、新生仔体重は平均的な新生
仔体重よりも上昇していた。なお、母獣には何ら病態的
な変化は認められなかった。When this feed was administered to pregnant cows and the effect of promoting fetal growth was examined, the weight of the newborn was higher than the average weight of the newborn. No pathological changes were observed in the dams.
【００１９】[0019]
【発明の効果】本発明の胎仔発育促進剤及び胎仔発育促
進効果を賦与した家畜飼料は、妊娠母獣の日常的な経口
摂取により、極めて安全に胎仔発育促進効果を発揮す
る。したがって、未熟仔出産を防止することができると
共に、新生仔の体重増加に寄与して家畜としての経済価
値を一層高めることができる。EFFECT OF THE INVENTION The fetal growth promoting agent of the present invention and the livestock feed to which the fetal growth promoting effect has been imparted can exert the fetal growth promoting effect extremely safely by daily oral ingestion of pregnant mothers. Therefore, it is possible to prevent the delivery of premature babies and to further increase the weight of the newborn babies, thereby further increasing the economic value as domestic animals.
Claims (4)
成分とする胎仔発育促進剤。1. A fetal growth promoter comprising a milk-derived basic protein composition as an active ingredient.
て塩基性アミノ酸を10重量％以上含有した乳由来の塩基
性タンパク質組成物を用いることを特徴とする請求項１
の胎仔発育促進剤2. A milk-derived basic protein composition having an isoelectric point of 7.5 to 11 and containing 10% by weight or more of a basic amino acid as a constituent amino acid is used.
Fetal growth promoter
して胎仔発育促進効果を賦与した家畜飼料。3. Livestock feed containing a milk-derived basic protein composition and imparting an effect of promoting fetal growth.
て塩基性アミノ酸を10重量％以上含有した乳由来の塩基
性タンパク質組成物を用いることを特徴とする請求項３
の家畜飼料4. A milk-derived basic protein composition having an isoelectric point of 7.5 to 11 and containing 10% by weight or more of a basic amino acid as a constituent amino acid is used.
Livestock feed
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28116297A JP3933273B2 (en) | 1997-09-30 | 1997-09-30 | Livestock feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28116297A JP3933273B2 (en) | 1997-09-30 | 1997-09-30 | Livestock feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH11103788A true JPH11103788A (en) | 1999-04-20 |
| JP3933273B2 JP3933273B2 (en) | 2007-06-20 |
Family
ID=17635225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP28116297A Expired - Fee Related JP3933273B2 (en) | 1997-09-30 | 1997-09-30 | Livestock feed |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3933273B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001231510A (en) * | 2000-02-22 | 2001-08-28 | Snow Brand Milk Prod Co Ltd | Seafood-paste |
| US7749960B2 (en) | 2001-04-03 | 2010-07-06 | Nestec S.A. | Osteoprotegerin in milk |
| WO2014157156A1 (en) * | 2013-03-28 | 2014-10-02 | 雪印メグミルク株式会社 | Protein composition and production process therefor |
| RU2663463C1 (en) * | 2016-07-04 | 2018-08-06 | Ришат Сальманович Исхаков | Method for increasing effectiveness of application of nutrient stimulants in fattening of young cattle |
-
1997
- 1997-09-30 JP JP28116297A patent/JP3933273B2/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001231510A (en) * | 2000-02-22 | 2001-08-28 | Snow Brand Milk Prod Co Ltd | Seafood-paste |
| US7749960B2 (en) | 2001-04-03 | 2010-07-06 | Nestec S.A. | Osteoprotegerin in milk |
| WO2014157156A1 (en) * | 2013-03-28 | 2014-10-02 | 雪印メグミルク株式会社 | Protein composition and production process therefor |
| JP2014193125A (en) * | 2013-03-28 | 2014-10-09 | Snow Brand Milk Products Co Ltd | Protein composition, and method for manufacturing the same |
| US9861121B2 (en) | 2013-03-28 | 2018-01-09 | Megmilk Snow Brand Co., Ltd. | Milk basic protein composition and production process therefor |
| RU2663463C1 (en) * | 2016-07-04 | 2018-08-06 | Ришат Сальманович Исхаков | Method for increasing effectiveness of application of nutrient stimulants in fattening of young cattle |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3933273B2 (en) | 2007-06-20 |
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