JPH0521004Y2 - - Google Patents

Description

[Detailed explanation of the invention] A. Purpose of the invention (industrial application field) This invention involves centrifuging the solids contained in a liquid and smearing them onto a glass slide in order to make a microscopic specimen of the solids contained in the liquid. This invention can be used as a cell device for centrifugal smearing.

(Prior Art) For pathological research and diagnosis, for example, when removing red blood cells from the blood of a subject, solid matter contained in a liquid is separated and smeared onto a glass slide. A centrifugal smear cell as shown in FIG. 9 is used.

This conventional centrifugal smearing cell (hereinafter simply referred to as a cell) 1 has a container tube 3 with an upwardly bent injection port coupled to a substrate 2, and a hole 4 of the container tube 3 is passed through the substrate 2 and the opposite side is formed. It has an opening on the side. 5 and 6 indicate reinforcing ribs.

To perform centrifugal smearing, the cell is attached to a holder and then placed in a centrifuge.

As illustrated in FIG. 10, the conventional holder 9 is mainly composed of a rectangular plate 11 with folded edges 11a formed on three sides. A hook 13 for locking a restraining spring 12 is formed. A groove 14 is formed around the rectangular plate 11 at the hem of the folded edge 11a.

To attach a cell or the like to the holder 9, as shown in FIG. is pressed against the holder 9, and the spring 12 is locked to the hook 13, so that the cell 1 and the like are connected to the holder 9 so as not to be separated.

The holder 9 with the cell 1 etc. attached in this way is attached to the centrifuge 15 as schematically shown in FIG. When rotated, the solid content centrifuged from the liquid to be treated in the hole 4 of the cell 1 adheres to the slide glass surface, and the liquid content is transferred to the water-permeable packing plate 7.
It passes through and is pushed out into the holder 9, accumulates in the groove 14 due to centrifugal force, and some of it is centrifuged from the upper part without the folded edge 11c (Fig. It is dispensed into the case 17 of the separator. After the centrifuge is stopped, these liquids flow down and accumulate at the bottom of the case, and are discharged from the discharge pipe 18.

After stopping the rotating shaft 16, the holder 9 is taken out from the centrifuge, the restraining spring 12 is removed, the slide glass is taken out, and it is transferred to a microscope specimen preparation process.

(Problem to be solved by the invention) The conventional holder constructed and used as described above is made of a metal plate so that it can be used repeatedly, and the cells are also used repeatedly. Cleaning was troublesome and required sterilization after cleaning.

This invention aims to provide a cell device with an attached holder that makes the cell and holder disposable and does not require troublesome processing such as cleaning.

B. Structure of the invention (means for solving problems) This invention consists of a container cylinder 24, a passage cylinder 23, a substrate 2
2 connected to each other, and a cell part 21 made of synthetic resin and having a passage tube 23 opened through the substrate 22.
and a holder part 30 made of synthetic resin and having a recess 33 for storing the centrifuged liquid in a rectangular plate 31 that matches the substrate 22, and the substrate 22 of the cell part is directed toward the rectangular plate 31 of the holder part. The cell part 21 and the holder part 30 are curved in a convex shape.
A cell device for centrifugal smearing is obtained in which the substrate 22 and the rectangular plate 31 are made to match and can be attached and detached freely, and after the centrifugal smearing process is completed, the cell part and the holder part are discarded as they are. It is something.

(Function) When a slide glass and a packing plate are stacked and inserted into the holder part, and the end of the board is locked in the holder part while stacking and pressing the board of the cell part onto the packing board, the spring action of the board of the cell part will cause the cell part to The outlet of the passage tube is pressed against the packing plate, and the slide glass and the outlet of the passage tube are brought into close contact with each other through the packing plate. When this is attached to a centrifuge and its rotating shaft is rotated at high speed, the solid content of the liquid to be treated is smeared onto the slide glass surface, and the liquid passes through a water-permeable packing plate and is pushed out to the holder. It adheres to the rectangular plate in layers. When the centrifuge is stopped, this adhered liquid flows down into the recess of the holder and is collected there.
Or it is absorbed by the absorbent liquid placed in the recess.

After the cell device with the holder attached thereto is removed from the centrifuge, the cell part and the holder part are opened, and the slide glass smeared with solid content is taken out, the cell device can be thrown away as is.

(Example) Figures 1 to 6 show the first example of this invention,
FIG. 1 is a perspective view of the cell part and holder part separated;
Fig. 2 is a vertical side view of the cell part and holder part combined, Fig. 3 is a plan view of the cell part, Fig. 4 is a bottom view of the holder part, and Fig. 5 is a right end view of the holder part. , FIG. 6 is a perspective view of the lock piece of the holder part as seen in the direction of arrow a in FIG. 4.

As shown in FIGS. 2 and 3, the cell part 21 has a substrate 22
It has a container cylinder 24 that communicates with a passage cylinder 23 that opens on the lower surface of the board, and a hole 25 is provided at the left end of the substrate 22.
A locking protrusion 26 is formed on the substrate 2 at the end of the hole 27.
A locking protrusion 28 and a handle 29 are formed at the right end of 2. Substrate 22, passage cylinder 23, container cylinder 24
are connected by reinforcing ribs 5 and 6.

The holder part 30 (FIGS. 4 and below) surrounds a rectangular plate 31 on three sides with folded edges 32, and forms a recess 33 at the end that becomes the lower part (left end in FIG. 2) when attached to a centrifuge. Two inclined grooves 34 communicate with the right end portion on the opposite side. A hole 35 into which the locking protrusion 26 of the cell portion is inserted is bored in the folded edge 32 at the left end. A hole 4 is provided at the right end of the rectangular plate 31 without a folded edge.
3, and a hook piece 37 is connected to the right end via two thin arms 36. A hook 38 is formed at the upper end of the hook piece 37. The hole 43 is used to push out the slide glass with a finger when taking it out.

The cell part 21 uses nylon 66 as a material to provide not only moldability but also strength and elasticity to the substrate 22, and the holder part 30 uses polyethylene to improve moldability.

To use this cell device with a holder, as shown in FIG.
A slide glass 8 is placed over the edge of the slide glass, a water-permeable packing plate 7 is placed on top of it, and a cell part 21 is placed on top of it. To overlap the cell parts 21,
Insert the locking protrusion 26 on the left end into the hole 35 of the holder part, and insert the locking protrusion 28 on the right end into the hook piece 37 of the holder part.
Press down towards. Since the hook piece 37 is connected to the holder part by a thin arm 36 that is easily elastically deformed, it easily swings, and the locking protrusion 28 pushes away the key 38 and moves below it, and when the key 38 returns to its position, the holder part 30
is locked.

The curvature and elastic deformation of the cell portion 21 are caused by the substrate 22
Since the area near the opening of the passage tube 23 is not easily deformed, the slide glass can be pressed uniformly in this area via the packing plate 7. The elasticity with which the substrate 22 presses against the slide glass can be adjusted not only by selecting the thickness of the substrate but also by the sizes of the holes 25 and 27. 39
is a lid that is attached to and detached from the mouth of the container cylinder 24. Lid 39
This allows air to enter the container cylinder during centrifugation by making pores in the container or by forming narrow grooves in the mouth of the container cylinder.

Once the cell part 21 is connected to the holder part 30 as described above, the liquid to be treated is poured into the container cylinder 24, the lid 39 is applied, and the holder part 30 is attached to a centrifuge and rotation is started. The solid content of the liquid can be smeared onto the glass slide 8 in the area of the holes 10 in the packing plate. The liquid pushed out around the water-permeable packing plate 7 passes through the inclined groove 34 or is directly collected in the recess 33 of the holder portion.

If a liquid-absorbent material such as paper or sponge is placed in the recess 33, there is no need to worry about spilling the liquid when removing the slide glass.

After removing the slide glass coated with the solid content, the cell portion and holder portion are discarded as they are.

Figures 7 and 8 show a second embodiment of this invention,
The cell part and the holder part are integrally molded. Parts that are equivalent to those in the first embodiment are given the same reference numerals and explanations will be omitted, and differences will be mainly explained.

The connection part between the cell part 21 and the holder part 30 is the eighth
As shown in the figure, it has a thin wall portion 40 that acts as a hinge, allowing the cell portion 21 to be folded over the holder portion 30.

A folded edge 42 with a hook 41 at the end is formed at one end of the cell part (the right end in the state shown in FIG. 7), and a slide glass 8 and a water-permeable packing plate 7 are placed on the holder part 30. When the thin wall portion 40 is folded and the cell portion is folded over the holder portion, the hook 41 is engaged with the bottom of the holder portion and the two portions are joined together.

C. Effects of the invention The cell device for centrifugal smearing of this invention (1) can be mass-produced at low cost by molding synthetic resin, so it can be disposed of after each centrifugal smear.

(2) Therefore, unlike conventional cells, there is no need for cleaning or sterilization after use.

(3) Since the structure is such that the liquid extruded from the water-permeable packing plate 7 is captured in the holder, air mist scattered inside the centrifuge can be eliminated. Therefore, even if the liquid contains harmful substances, the risk of harm from them is prevented.

[Brief explanation of the drawing]

Figures 1 to 6 show the first embodiment of this invention, Figure 1 is an exploded perspective view of the cell part and holder part, and Figure 2 is a longitudinal cross-sectional side view of the cell part and holder part combined. Figure 3 is a plan view of the cell part, Figure 4 is a bottom view of the holder part, Figure 5 is a right end view of the holder part,
FIG. 6 is a perspective view of the hook piece seen in the direction of arrow a in FIG. 4, and FIGS. 7 and 8 show a second embodiment of this invention.
FIG. 7 is an overall perspective view, FIG. 8 is a longitudinal cross-sectional side view of the thin wall portion 40, FIGS. 9 to 12 show a conventional cell and holder, FIG. 9 is a perspective view of the cell, and FIG. 10 is a holder. A perspective view, FIG. 11 is a vertical side view schematically showing a state in which the cells are attached to a holder, and FIG. 12 is a vertical side view schematically showing the centrifugal separator. DESCRIPTION OF SYMBOLS 1... Cell for centrifugal smearing, 2... Substrate, 3... Container tube, 4... Hole, 5, 6... Reinforcement rib, 7... Packing plate, 8... Slide glass, 9... Cell holder, 10 ...hole, 11...rectangular plate, 11a...
...Folding edge, 12...Pressure spring, 13...Hook,
14... Groove, 15... Centrifugal separator, 16... Rotating shaft, 17... Case, 18... Discharge pipe, 21...
Cell portion, 22... substrate, 23... passage tube, 24...
... Container cylinder, 25 ... Hole, 26 ... Locking projection, 27
... Hole, 28 ... Locking protrusion, 29 ... Handle, 30
...Holder part, 31 ... Rectangular plate, 32 ... Folded edge, 33 ... Recessed part, 34 ... Inclined groove, 35 ...
Hole, 36... Thin arm, 37... Key piece, 38... Key, 39... Lid, 40... Thin wall part, 41... Key, 42... Folded edge, 43... Hole.

Claims (1)

[Claims for Utility Model Registration] A cell portion 21 made of synthetic resin and formed by connecting a container tube 24, a passage tube 23, and a substrate 22, with the passage tube 23 opening through the substrate 22, and a substrate 22. It consists of a matching rectangular plate 31 and a synthetic resin molded holder part 30 with a concave part 33 for storing the centrifuged liquid, and the cell part's substrate 22 is curved in a convex shape toward the holder's rectangular plate 31. and the cell part 21 and the holder part 30
A cell device for centrifugal smearing is made up of a substrate 22 and a rectangular plate 31 which are made to match and can be attached and detached freely.