JPS6339647Y2 - - Google Patents

Description

[Detailed Description of the Invention] This invention relates to a cell device for centrifugal smearing consisting of a cell and a cell holder. A centrifugal smear method is used to smear solids contained in a liquid onto a glass slide to create a microscopic specimen. In this method, a cell injected with a liquid containing solids is stacked on a slide glass and held in a holder, which is then attached to a centrifuge and centrifuged over the slide glass located on the outer periphery of the centrifuge. In this method, solid matter is deposited, and the solid matter is separated and applied to a slide glass at the same time.

In the conventional centrifugal smear cell device, a cell 1 shown in FIG. 1 is attached to a slide glass 3 through an elastic packing plate 2 such as silicone rubber shown in FIG.
(Fig. 3) and was attached to the cell holder 4 (Fig. 4).

The cell 1 has a substrate 5 of the same shape and size as the slide glass 3, and a bent tubular container tube 6 formed on one side thereof, and a bent hole 7 of the container tube 6 penetrates to the opposite side of the substrate 5. It is. 5a and 5b are reinforcing ribs. The packing plate 2 includes the substrate 5 of the cell 1.
When overlapped, a hole 8 is formed which matches the hole 7 and has the same shape and size. The cell holder 4 shown in FIG. 4 is made by folding up three edges of a slide glass 3 and a bottom plate 9, which has the same shape and size as the packing plate 2, and then folding up two folds near the open front side (right side in FIG. 4). Hooks 10, 10 are formed on the edges, and a lateral tubular spring receiver 11 is provided on the rear folded edge. 5th to 6th
As shown in the figure, the spring 12 can be inserted into the spring receiver 11 from both ends by elastically opening the rear part 13.
A stopper 14 is fitted to the front end so as to be slidable in the front-rear direction, and the middle portion is bent downward. These cells 1, packing plate 2, and slide glass 3 are
Place the holder 4 on the seat plate 9 of the holder 4 in a stacked manner as schematically shown in the figure, hold it down with the restraining spring 12, and attach the stopper 14 to the hook 10.
They are held in a stacked state.

To process liquids using this cell device,
As schematically shown in FIG. 8, the holder 4 is attached to the side of the centrifugal separator 15 with the cell hole 7 containing the liquid facing upward, and the centrifugal separator 15 is rotated at high speed. The solid content in the liquid moves toward the glass slide 3 due to centrifugal force and adheres to the glass slide. Due to the packing plate 2, the liquid does not leak out of the holes 8.
Then, the separator is stopped, the cell device is taken out, the liquid remaining in the cell 1 is drained, the metal fitting 14 is removed from the hook 10, the restraint by the restraining spring 12 is released, and the cell 1, packing plate 2, and slide glass are separated. A glass slide smeared with solids is obtained. This slide glass is made into a microscopic specimen through subsequent processing such as staining and attaching a cover glass.

The conventional centrifugal smearing cell device as described above uses a large plate-shaped packing plate 2, so when separating it from the cell substrate 5 and slide glass 3, it is difficult to use the correct hand when separating it from the cell substrate 5 and slide glass 3. The operation is difficult because the smeared solid content is rubbed off by the packing plate 2. In addition, when holding or removing cells etc. with the spring 12, the stop 14 is slid against the spring 12, which increases the number of operations and makes it difficult to operate with one hand. It also had the disadvantage of being troublesome.

This invention was devised for the purpose of improving such a conventional cell device, eliminating the above-mentioned drawbacks, and obtaining a cell device for centrifugal smearing that is easy to operate and easy to manufacture.

Next, the centrifugal smearing cell device of this invention will be explained with reference to FIGS. 9 to 16. Since this device is an improved version of the conventional device described above, parts equivalent to those of the conventional device are designated by the same reference numerals, and their explanation will be omitted.

As shown in FIGS. 9 and 10, in the cell 1, a recess 16 is formed around the opening on the lower surface of the substrate 5 where the hole 7 of the container cylinder 6 opens, and an annular packing 17 is fitted into the recess 16. The annular section of the packing 17 has a rectangular cross section with a seat 17a that fits into the recess 16 and a protrusion 17b that projects from the bottom surface of the substrate 5. matches.

The hook of the cell holder 4 is made smaller than the conventional one to the extent that it can hook the wire forming the restraining spring, as shown in FIGS. 11 and 12. That is, it is formed in a triangular shape with the hypotenuse facing upward, and its protruding length is not as long as the conventional hook 10.

The restraining spring 12a is formed as shown in FIGS. 13-14. The rear parts 13, 13 of the spring are elastically opened so as to be inserted into both ends of the spring receiver 11 of the holder, and the intermediate part 18 is bent downward to hold the cell substrate 5 toward the bottom plate 9 of the holder. This is similar to the conventional spring 12, but the shape of the front part is different. That is, a closed square is formed at the bent and crossed ends of the intermediate parts 18, 18, and the locking part 19 is formed.
That is. This restraining spring 12 is the thirteenth
When the intermediate portion 18 is pushed in the direction of the arrows 20, 20 in the figure, the locking portion 19 moves forward in the direction of the arrow 21. The holder 4 with this restraining spring 12a attached to the bottom plate 9 is as shown in FIG.

When the cell 1 formed as described above is stacked on the slide glass 3 and on the bottom plate 9 of the cell holder, and the restraining spring 12a is pushed toward the bottom plate 9, the bent middle part pushes the cell substrate 5 onto the bottom plate 9 of the holder. At the same time, the locking part 19 hits the slope of the hook 10a and descends while slightly elastically deforming the spring 12a, and when it passes the position of the hook 10a, it returns to its original shape and the locking part 19 hits the hook 10a. The cell 1 and slide glass 3 are locked and cannot be removed, and the cell 1 and the slide glass 3 are coupled to the cell holder 4. Therefore, when a liquid is injected into the container cylinder 6 of the cell, the holder 4 is attached to a centrifuge 15, and the liquid is centrifuged, the solid content in the liquid is separated and adhered to the surface of the slide glass. During centrifugation, the liquid does not leak out of the packing because the protrusion 17b of the packing slightly protrudes from the cell substrate 5 and comes into elastic contact with the slide glass.

When removing the cell 1 and slide glass 3 from the cell holder 4, press the intermediate portions 18, 1 of the restraining spring 12a.
8 in the direction of the arrows 20, 20, the locking part 19 moves slightly in the direction of the arrow 21, so the locking part 19 comes off the hook 10a and moves upward due to the elasticity of the spring 12a. , cell 1 and slide glass 3 can be removed from the holder.

In the centrifugal smearing cell device of this invention, an annular packing 17 is fitted to the base plate 5 of the cell to prevent the liquid from leaking out of the hole 7 of the container cylinder 6. Since the packing plate 2 is not used, it is difficult to make an erroneous operation such as rubbing the smeared surface of the slide glass and ruining the solid content to be tested when removing the packing plate 2. In addition, the hook 10a of the holder and the restraining spring 12a are The improvements have been made so that the restraining spring can be attached and detached from the hook 10a with a single finger movement of one hand, making it easier to handle.
This has a great effect in allowing centrifugal smear processing to be performed easily and without errors.

[Brief explanation of drawings]

Figures 1 to 8 illustrate a conventional centrifugal smearing cell device. Figure 1 is a perspective view of the cell, Figure 2 is a perspective view of the packing plate, Figure 3 is a perspective view of the slide glass, and Figure 4 is a perspective view of the packing plate. The figure is a perspective view of the cell holder without the restraining spring, Figure 5 is a plan view of the restraining spring, Figure 6 is a side view of the restraining spring, and Figure 7 is a schematic illustration of the cell, slide glass, etc. attached to the holder. 8 is a vertical sectional view schematically showing the centrifuge, FIG. 9 and the following show the cell device of this invention, FIG. 9 is a side view of the cell, and FIG. 10 is a section A in FIG. 9. , FIG. 11 is a side view of the cell holder, FIG. 12 is a plan view of the cell holder, FIG. 13 is a plan view of the restraining spring, FIG. 14 is a side view of the restraining spring, and FIG. 15 is a side view of the cell holder. The perspective view and FIG. 16 are side views of the cell holder when the restraining spring is locked to the hook. 1: Cell, 2: Packing plate, 3: Slide glass, 4: Cell holder, 5: Substrate, 5a, 5b:
Reinforcement rib, 6: Container tube, 7, 8: Hole, 9: Seat plate,
10: Hook, 11: Spring receiver, 12, 12a:
Retaining spring, 13: Rear, 14: Stop, 15: Centrifugal separator, 16: Recess, 17: Packing, 17
a: seat portion, 17b: protrusion, 18: intermediate portion, 19:
Locking portions, 20, 21: arrows.

Claims (1)

[Scope of utility model registration request] A bent tubular container tube 6 is integrally formed on one side of a substrate 5 having the same shape and size as the slide glass 3, and the bent hole 7 of the container tube 6 is passed through to the opposite side of the substrate 5, and this hole of the substrate 5 is formed. A cell 1 is formed by forming a recess 16 around the opening of 7 and fitting an annular packing 17 thereinto, with a part of the packing 17 protruding from the substrate 5, and a bottom plate having the same shape and size as the slide glass 3. Fold up the three edges of 9, and make triangular hooks 10a, 10a with the hypotenuses upward, protruding from the two folded edges close to one side of the open side. A tubular spring receiver 11 is formed, one end is elastically opened and inserted into both ends of the spring receiver 11, the middle parts 18, 18 are bent toward the bottom plate 9, and the other end is made to cross by bending the middle part, A cell device for centrifugal smearing comprising a cell holder 4 having a closed rectangular locking part 19 formed at its tip.