JPH0540432Y2 - - Google Patents

Description

[Detailed description of the invention] (Industrial application field) This invention is used to centrifuge the solids contained in a liquid and smear it on a glass slide in order to make a microscopic specimen of the solids contained in the liquid. This invention can be used for a holder used to support a centrifugal smear cell holder and attach it to a centrifuge.

(Prior art) For pathological research and diagnosis, for example, when removing red blood cells from the blood of a subject, the eighth method is used to separate solids contained in a liquid and smear it on a slide glass. A cell holder for centrifugal smearing is used as shown in the figure.

This centrifugal smear cell holder (hereinafter simply referred to as a cell) 1 has a container tube 3 with an upwardly bent injection port coupled to a substrate 2, and a hole 4 of the container tube 3 is inserted through the substrate 2 to the opposite side. It is left open. 5 and 6 are reinforcing ribs.

To perform centrifugal smearing, as shown in FIG. 9, first, the substrate 2 of this cell 1 is stacked on a slide glass 8 through a liquid-permeable packing plate 7 such as filter paper, and placed in a cell holder (hereinafter simply referred to as holder). 9), which is then attached to a centrifugal separator as described below. The packing plate 7 is provided with holes 10 corresponding to the holes 4 of the cells 1.

As illustrated in FIG. 10, a conventional holder 9 has a rectangular back plate 1 with raised edges 11a on three sides.
1 as the main body, the spring 1 is held down by the lower folding edge 11a.
2 is pivotally mounted, and the springs 12 are pressed against the folded edges 11a on both sides.
A hook 13 for locking is formed. A groove 14 is formed around the back plate 11 at the hem of the folded edge 11a.
is formed, and a hole 15 is bored in the groove 14.

To attach a cell, etc. to the holder 9, as shown in FIG. The spring 12 is engaged with the hook 13 to connect the cell 1 and the like to the holder 9 so that they will not separate.

As schematically shown in FIG. 11, the holder 9 with the cells 1 etc. connected thereto is attached to the peripheral wall of the rotor 17 of the centrifugal separator 16 in such a way that centrifugal force acts perpendicularly to the back plate 11. When an appropriate amount of the liquid to be treated is injected into the hole 4 of the cell and the rotating shaft 18 is rotated at high speed, the cell 1
The solid content centrifuged from the liquid to be treated in the hole 4 adheres to the slide glass surface, and the liquid passes through the liquid-permeable packing plate 7 and is pushed out of the cell from its end. Thereafter, the rotation of the shaft 18 is stopped, the holder 9 is taken out from the centrifuge, the restraining spring 12 is removed, the slide glass is taken out, and this is transferred to a microscope specimen preparation process.

The liquid that is extruded through the liquid-permeable packing plate 7 during centrifugation enters the groove 14 of the holder, and if there is no hole 15, the entire amount would be shaken into the centrifuge 16 from the upper part without the folded edge 11a. It is blown away and discharged from the reservoir discharge pipe 20 at the bottom of the case 19. but,
During centrifugation when the rotating shaft 18 rotates at high speed, the liquid shaken off from the top of the holder 9 scatters as air mist inside the case 19 and also adheres to the lid 21. When the centrifuge is stopped and the lid 21 is opened to take out the holder 9, etc., the liquid adhering to the lid drips from the lid 21 and contaminates the exterior of the centrifuge, the desk, etc. This liquid may contain harmful substances such as bacteria and dirt, so
This condition of dripping from the lid must be avoided.

The hole 15 formed in the groove 14 is designed to reduce the amount of liquid that scatters from the top of the holder, which has no folded edges, and reaches the lid 21.
100084), the liquid that entered the groove 14 comes out of the holder through the hole 15 while rising in the groove.
It adheres to the side surface of the case of the centrifuge at a position away from the lid 21, making it difficult to reach the lid 21.

(Problem to be solved by the invention) The holder with the hole 15 shown in Fig. 10 minimizes the amount of liquid adhering to the lid of the centrifuge, but the invention aims to eliminate this adhesion. Another object of the present invention is to obtain a holder that can prevent the generation of air mist.

(Means for solving the problem) This idea forms a continuous folded edge around the four circumferences of a rectangular back plate, surrounds the entire circumference of the back plate, and provides a support part for separating the slide glass from the back plate. In addition to providing
A reservoir for the liquid separated from the liquid to be processed is provided at the bottom of the back plate, and the liquid extruded from the liquid-permeable packing plate is collected in this reservoir and used in the centrifuge. This prevents it from scattering inside the case.

(Function) The liquid extruded from the liquid-permeable packing plate on all four sides is prevented from exiting the holder due to the folded edges on the four sides, and adheres to the back plate due to centrifugal force, stopping the centrifuge. It flows down along the back plate, enters the reservoir, and is taken out from the centrifuge case together with the holder. Prevents liquid from scattering and staining the case and lid.

(Example) 1 and 2 show a first embodiment of the invention.

The cell holder 22 has a folded edge 22a continuous to the four circumferences of a rectangular back plate 11 similar to the holder 9 in FIG.
A support part 23 in the form of a partition plate that partitions the lower part of the holder and a support part 24 in the form of a projecting piece are formed in the intermediate part thereof to protrude into the folded edge. Groove 14 is not necessary. The other structure is the same as the holder 9 in FIG. 10. Support parts 23 and 24 of this holder 11
As shown by the chain lines in FIG. 2, the slide glass 8, liquid-permeable packing plate 7, and cell 1 substrate 2 are stacked and held down by the restraining spring 12, and the slide glass 8, cell 1, etc. are placed in the holder 22. Since they are connected, connect them to the rotor 1 of the centrifuge 16 as shown in FIG.
7, cover the shaft 1 of the rotor 17 with the cover 21
Rotate 8.

As a result, the solid content in the liquid to be treated is smeared onto the slide glass 8, and the liquid passes through the liquid-permeable packing plate 7 and is pushed out from the end thereof into the holder 22. This leaked liquid is absorbed by the four folded edges 22a.
Therefore, the support part 2 does not come out of the holder due to centrifugal force.
3 and 24 and adheres to the inner surface of the back plate 11 of the holder as if coated.

When the rotating shaft 18 is stopped after centrifugation, this attached liquid flows down the inner surface of the back plate and collects in the reservoir 25 surrounded by the support part 23, the back plate 11, and the lower folded edge 22a, and the cells are centrifuged. It is taken out together with it when it is removed from the machine. In this way, most of the liquid in the liquid to be processed is captured within the holder.

FIG. 3 shows a second embodiment of the present invention, in which support portions 23, 24 are arranged inwardly from the four circumferential folded edges 22a.
, and protrusions 26 are formed on the left and right sides of the outer surface of the folded edge 22a.
The holder 22 is made by forming the holder 22, and separately, the four circumferences are folded up, and the left and right folded edges are made to protrude a little more to form the protrusion 26.
A box 28 is formed with a groove 27 that engages with the groove 2.
By engaging 7 with the protrusion 26, the holder 2
2 and box 28 can be freely combined into one box shape, and is a separable version of the structure shown in FIGS. 1 and 2.

In this way, by separating the box 28 from the holder 22, the holder and the box can be easily cleaned after use.

FIG. 4 shows a third embodiment of the present invention, in which instead of forming the reservoir 25 by the large support part 23 as in the previous example, a through hole 29 is bored in the lower part of the back plate 11.
The edge of this hole is made to protrude behind the back plate 11, and the mouth of the blow-molded container 30 is fitted into this to form a reservoir.

During centrifugation, the separated liquid enters container 30 through hole 29 and is captured.

In the fourth embodiment shown in FIGS. 5 and 6, a table-shaped support portion 32 for supporting a slide glass 8 is extruded from the bottom surface of the back plate 11 of a holder 31 whose four circumferences are folded up in the same way as in FIG. 1. , slide glass 8 during centrifugation
A space is formed between the back plate 11 and the back plate 11, and a reservoir 33 for absorbing paper, sponge, etc. is placed in this space. The liquid extruded from the packing plate 7 during centrifugation and collected in the holder due to the four folded edges adheres to the back plate of the holder, which is installed vertically along the side wall of the case of the centrifuge. When the centrifuge is stopped, the water flows down and is absorbed into the reservoir 33. After the centrifugation process, the reservoir containing the liquid is discarded.

The fifth embodiment shown in FIG.
A support portion 32 that extends from 2 and continues to the upper folded edge.
a, and its action is the same as the previous example.

(Effects of the invention) As can be seen in the above embodiments, the cell holder of the present invention conventionally scatters as air mist from the top of the holder installed vertically around the centrifuge, contaminating the inside of the centrifuge. The folded edge provided on the upper part allows the liquid to stay within the holder without scattering outside the holder, and a reservoir is provided to accommodate the liquid that flows down when the centrifuge is stopped. It is possible to prevent the liquid from becoming air mist and scattering inside the centrifuge.

Therefore, even if the liquid contains harmful substances, the risk of harm from them can be eliminated.

Note that an apparatus for applying a sample to a slide glass is described in Japanese Patent Application Laid-Open No. 126227/1982, but this does not use a cell holder and moves the sample using a weak centrifugal force to form thin stripes on the slide glass surface. This is different from the cell holder of the present invention.

[Brief explanation of the drawing]

Figures 1 to 7 show five embodiments of the present invention, Figure 1 is a perspective view of the first embodiment, Figure 2 is a vertical side view thereof, and Figure 3 is an exploded perspective view of the second embodiment. , 4th
The figure is a longitudinal sectional side view of the third embodiment, FIG. 5 is a perspective view of the fourth embodiment, FIG. 6 is a longitudinal sectional side view thereof, and FIG. 7 is a perspective view of the fifth embodiment. 8 to 11 show a conventional cell and cell holder, FIG. 8 is a perspective view of the cell, FIG. 9 is a vertical side view schematically showing how it is attached to the cell holder, and FIG. 10 is a perspective view of the cell holder. FIG. 11 is a schematic longitudinal sectional side view of the centrifuge. DESCRIPTION OF SYMBOLS 1... Cell for centrifugal smearing, 2... Substrate, 3... Container tube, 4... Hole, 5, 6... Reinforcement rib, 7... Packing plate, 8... Slide glass, 9... Cell holder, 10 ... Hole, 11 ... Back plate, 11a ...
Folding edge, 12...Pressure spring, 13...Hook, 1
4... Groove, 15... Hole, 17... Centrifugal separator, 1
7... Rotor, 18... Rotating shaft, 19... Case, 20... Discharge pipe, 21... Lid, 22... Cell holder, 22a... Folding edge, 23, 24... Support part, 25... Reservoir Part, 26... Projection, 27...
Groove, 28...Box, 29...Through hole, 30...Container,
31... Holder, 32, 32a... Support part, 33
...Reservoir.

Claims (1)

[Claims for Utility Model Registration] A slide glass 8, a liquid-permeable packing plate 7 with holes 10, and a cell substrate 2 are stacked on a back plate 11, and a centrifugal force acts perpendicularly to the back plate 11. This is a cell holder for centrifugal smearing to be attached to a centrifuge, and the back plate 11 of the cell holder has a folded edge 22 surrounding the stacked glass slides, packing plate, and substrate around the four circumferences.
support portions 23, 24, 3 that support the slide glass apart from the back plate 11 within the folded edge 22a.
Reservoir parts 25, 30, 33 which are provided in the space between the back plate 11 and the support part or communicated with this space to accommodate the liquid separated from the liquid to be processed during centrifugation. A cell holder for centrifugal smearing, characterized in that it is provided with.