JPH0521003Y2 - - Google Patents
Info
- Publication number
- JPH0521003Y2 JPH0521003Y2 JP10008385U JP10008385U JPH0521003Y2 JP H0521003 Y2 JPH0521003 Y2 JP H0521003Y2 JP 10008385 U JP10008385 U JP 10008385U JP 10008385 U JP10008385 U JP 10008385U JP H0521003 Y2 JPH0521003 Y2 JP H0521003Y2
- Authority
- JP
- Japan
- Prior art keywords
- hole
- cell
- liquid
- vertical
- hole portion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000758 substrate Substances 0.000 claims description 7
- 210000004027 cell Anatomy 0.000 description 20
- 239000007788 liquid Substances 0.000 description 20
- 239000007787 solid Substances 0.000 description 10
- 238000005119 centrifugation Methods 0.000 description 8
- 239000011521 glass Substances 0.000 description 8
- 238000012856 packing Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000001746 injection moulding Methods 0.000 description 3
- 229920003002 synthetic resin Polymers 0.000 description 3
- 239000000057 synthetic resin Substances 0.000 description 3
- 238000000465 moulding Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
Landscapes
- Centrifugal Separators (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Microscoopes, Condenser (AREA)
Description
【考案の詳細な説明】
イ 考案の目的
（産業上の利用分野）
この考案は、液体中に含まれる固形分の顕微鏡
標本を作るために、固形分を遠心分離してスライ
ドガラス上に塗抹するのに使用する遠心塗抹用セ
ルに利用できる考案であつて、特にスライドガラ
スとの間に介在させるパツキンングとして紙を
使用する場合に利用できる考案である。[Detailed explanation of the invention] A. Purpose of the invention (industrial application field) This invention involves centrifuging the solids and smearing them on a glass slide in order to prepare a microscopic specimen of the solids contained in the liquid. This device can be used in centrifugal smear cells used in the present invention, and is especially applicable when paper is used as the packing between the slide glass and the slide glass.
（従来の技術）
病理研究や診断のために、例えば被検者の血液
から赤血球を取出す場合のように、液体中に含ま
れる固形分を分離してスライドガラス上に塗抹す
るのに、遠心塗抹用セルが使用されている。(Prior art) Centrifugal smearing is used to separate solids contained in a liquid and smear it on a glass slide, for example when extracting red blood cells from the blood of a subject, for pathological research or diagnosis. cell is used.
第４図に略示するように、従来の遠心塗抹用セ
ル（以下、単にセルと称する）１は、基板２に、
注入口を上方に曲げた容器筒３を結合し、容器筒
３の通孔４を基板２を貫通して反対側に開口させ
たものである。 As schematically shown in FIG. 4, a conventional centrifugal smear cell (hereinafter simply referred to as cell) 1 has a substrate 2,
A container tube 3 with an upwardly bent injection port is joined together, and a through hole 4 of the container tube 3 penetrates the substrate 2 and opens on the opposite side.
遠心塗抹を行なうには、第４図のように、基板
２を、通孔４に対応する孔５を設けたパツキング
板６を介してスライドガラス７に重ねてホルダ８
に入れ、抑えばね９でこの結合状態を保持して、
第５図のように遠心分離機１０に取付け、セルの
通孔４に適量の被処理液を注入し、回転軸１１を
高速回転させる。これにより、被処理液中の固形
分が遠心分離されてスライド面に付着するから、
遠心分離機１０を停止させ、ホルダ８を取出して
通孔４中の液体を流し出し、固形分の塗抹された
スライドガラス７を取出して顕微鏡標本に作るの
である。 To perform centrifugal smearing, as shown in FIG.
and hold this connected state with the restraining spring 9.
As shown in FIG. 5, it is attached to a centrifuge 10, an appropriate amount of liquid to be treated is injected into the through hole 4 of the cell, and the rotating shaft 11 is rotated at high speed. As a result, the solid content in the liquid to be treated is centrifuged and adheres to the slide surface.
The centrifugal separator 10 is stopped, the holder 8 is taken out, the liquid in the through hole 4 is poured out, and the slide glass 7 smeared with solids is taken out and prepared as a microscope specimen.
上記の従来のセルでは、残留する液体分を流し
出すときに、スライドガラスに付着した固形分の
一部をも流してしまう。これを改善する手段とし
て、パツキング板に紙を使用すると、遠心分離
時に紙を透過して液体分が遠心分離機中に排出
され、排出管１６から排出されるので、固形分の
流出がなくなる。 In the conventional cell described above, when the remaining liquid is flushed out, a portion of the solid matter adhering to the slide glass is also flushed away. As a means to improve this, if paper is used for the packing board, the liquid permeates through the paper during centrifugation and is discharged into the centrifugal separator and then discharged from the discharge pipe 16, thereby eliminating the outflow of solids.
しかしながら、被処理液を注入して直ちに遠心
分離を行なわないと、待機時間中に固形分が液体
分と共に紙にしみ込んでしまい、遠心分離を開
始したときに、捕集できる固形分が減つてしま
う。 However, if centrifugation is not performed immediately after injecting the liquid to be processed, the solid content will soak into the paper along with the liquid content during the waiting period, reducing the amount of solid content that can be collected when centrifugation is started. .
本考案者は、この点を改善するために第６〜７
図のようにセルの孔４に溜り部１２を設けて遠心
分離を待機する間は、被処理液が紙のパツキン
グ板６に吸収されないものを考案（実願昭60−
52129号）した。１３，１３は補強リブである。 In order to improve this point, the present inventor proposed the 6th and 7th
As shown in the figure, a reservoir 12 is provided in the hole 4 of the cell so that the liquid to be treated is not absorbed by the paper packing plate 6 while waiting for centrifugation (Utility Application 1986-
No. 52129). 13, 13 are reinforcing ribs.
（考案が解決しようとする問題点）
第６〜７図のセルは、被処理液が紙に吸取ら
れてしまうことを防止できて、性能上は満足すべ
きものであるが、セルを合成樹脂で射出成型する
場合に、通孔４が屈曲しているので中子抜きがで
きないという製作上の欠点がある。これを造るに
は、リブ１３，１３を含む平面、又はこれに平行
な垂直平面で切断した形状の２個の部分片を造
り、これを接着すれば造れるが、これでは製造能
率が悪く単価も上昇する。(Problems to be solved by the invention) The cell shown in Figures 6 and 7 can prevent the liquid to be treated from being absorbed by the paper and is satisfactory in terms of performance, but the cell is made of synthetic resin. When injection molding is performed, there is a manufacturing disadvantage in that the core cannot be removed because the through hole 4 is bent. To make this, you can make two pieces cut on a plane that includes the ribs 13, 13, or a vertical plane parallel to this, and then glue them together, but this would result in poor manufacturing efficiency and low unit costs. Rise.
ロ 考案の構成
（問題点を解決するための手段）
この考案は、セル内の通孔４を、交叉する縦お
よび横の二つの真直な通孔部分により形成し、両
通孔部分の交叉部付近に、縦の通孔部分の下端部
により溜り部を形成するものであつて、溜り部を
持ちしかも製作容易なセルを得たものである。B. Structure of the device (means for solving problems) In this device, the through hole 4 in the cell is formed by two vertical and horizontal straight through hole portions that intersect, and the intersection portion of both through hole portions is A reservoir is formed near the lower end of the vertical through-hole portion, and a cell that has a reservoir and is easy to manufacture is obtained.
（作用）
セルの成型時に、二つの真直な通孔部分を形成
する中子を抜取ることは容易である。また、両通
孔部分の交叉部の下方に通孔部分の一部を突出さ
せることにより、被処理液の溜り部を形成するこ
ともできる。(Function) When molding the cell, it is easy to remove the core forming the two straight through-hole portions. Further, by causing a portion of the through-hole portion to protrude below the intersection of both through-hole portions, a reservoir portion for the liquid to be treated can be formed.
（実施例）
第１図は、本考案の第一実施例を示す斜視図、
第２図は第１図のＢ−Ｂ断面図、第３図は本考案
の第二実施例を示す第２図同様の断面図である。(Embodiment) FIG. 1 is a perspective view showing a first embodiment of the present invention;
2 is a sectional view taken along line BB in FIG. 1, and FIG. 3 is a sectional view similar to FIG. 2, showing a second embodiment of the present invention.
以下、前述の従来例と同等部分には同符号を使
用し、説明を省略して、本考案の実施例を説明す
る。 Hereinafter, an embodiment of the present invention will be described, using the same reference numerals for parts equivalent to those of the conventional example described above and omitting the explanation.
第１〜２図において、容器筒３は、横の部分３
ａと、縦の部分３ｂとから形成され、両部分内に
交叉する通孔部分４ａ，４ｂが形成されている。
この実施例では、基板２、通孔部分４ａ，４ｂの
交叉角を90°にしているが、90°に限定する必要は
ない。特に縦の通孔部分４ｂは、横の通孔部分４
ａより少し下方まで延して溜り部１４を形成させ
ている。溜り部１４の側面は横の通孔部分４ａに
傾斜面により接続させるのが好ましい。 In Figs. 1 and 2, the container cylinder 3 has a horizontal portion 3.
a and a vertical portion 3b, and intersecting through hole portions 4a and 4b are formed in both portions.
In this embodiment, the intersecting angle between the substrate 2 and the through-hole portions 4a and 4b is 90°, but it is not necessary to limit it to 90°. In particular, the vertical through hole portion 4b is different from the horizontal through hole portion 4b.
A reservoir 14 is formed extending slightly below a. It is preferable that the side surface of the reservoir portion 14 be connected to the lateral through-hole portion 4a by an inclined surface.
このようにセルを形成するから、縦の通孔部分
４ｂにピペツト等で適量の被処理液を注入する
と、被処理液は一応溜り部１４に溜り、横の通孔
部分４ａに流出しない。従つて被処理液注入後、
遠心分離を開始するまでに時間がかかつても、被
処理液が紙のパツキング板６（第４図参照。第
１〜２図には省略。）に吸取られてしまうことは
ない。 Since the cells are formed in this manner, when an appropriate amount of the liquid to be treated is injected into the vertical through-hole portion 4b using a pipette or the like, the liquid to be treated will temporarily accumulate in the reservoir portion 14 and will not flow out into the horizontal through-hole portion 4a. Therefore, after injecting the liquid to be treated,
Even if it takes some time to start centrifugation, the liquid to be treated will not be absorbed by the paper packing plate 6 (see FIG. 4, omitted from FIGS. 1 and 2).
遠心分離を始めると、被処理液は、縦の通孔部
分４ｂの側面に沿つて横の通孔部分４ａに入り、
従来同様に固形分の遠心分離、スライドガラスへ
の塗抹が行なわれる。このとき、被処理液の一部
は縦の通孔部分４ｂの上方にまで達し、セル外に
振り飛ばされて遠心分離機の蓋１０ａに付着し、
遠心分離終了後、蓋を外して机の上等に置いてた
りすると、被処理液（有害な細菌等を含むことが
多い）がたれ落ちて不潔である。縦の通孔部分４
ｂに蓋１５を施せば、この不都合は除ける。 When centrifugation is started, the liquid to be treated enters the horizontal through hole portion 4a along the side surface of the vertical through hole portion 4b,
The solid matter is centrifuged and smeared onto a glass slide in the same manner as before. At this time, a part of the liquid to be treated reaches above the vertical through hole portion 4b, is blown out of the cell, and adheres to the lid 10a of the centrifuge.
After centrifugation, if you remove the lid and leave it on a desk, etc., the liquid to be processed (often containing harmful bacteria) will drip out and become unclean. Vertical hole part 4
This inconvenience can be avoided by providing a cover 15 to b.
第３図は、縦の通孔部分４ｂを、上部が基板２
から離れる方向に傾斜させた本考案の第二実施例
を示し、横の通孔部分４ａより断面形を大きくし
た縦の通孔部分４ｂの下部が横の通孔部分４ａよ
り下方に形成する凹部が溜り部１４となる。この
場合は遠心分離の際に被処理液が縦の通孔部分４
ｂを上昇してセル外に振り飛ばされ危険は少な
い。 FIG. 3 shows the vertical through hole portion 4b with the upper part facing the substrate 2.
A second embodiment of the present invention is shown in which the vertical through hole portion 4b has a larger cross-sectional shape than the horizontal through hole portion 4a, and the lower part of the vertical through hole portion 4b is formed below the horizontal through hole portion 4a. becomes the reservoir 14. In this case, during centrifugation, the liquid to be processed is
There is little danger of being thrown out of the cell by climbing b.
このように通孔部分４ａ，４ｂを交叉させて通
孔４とすると共に溜り部１４を形成するから、射
出成型時に通孔形成のための中子の抜取りが容易
であり、迅速に操業できて製作費を低廉にするこ
とができる。 Since the through-hole portions 4a and 4b are crossed to form the through-hole 4 and the reservoir 14 in this manner, it is easy to remove the core for forming the through-hole during injection molding, and operations can be carried out quickly. Production costs can be reduced.
ハ 考案の効果
(1) 二つの真直な通孔部分４ａ，４ｂを交叉させ
て通孔４を造るから、中子の抜取りが自由にで
きて、合成樹脂による通孔成型が容易であり、
しかも同時に溜り部１４を形成することができ
る。C. Effects of the invention (1) Since the through hole 4 is made by intersecting the two straight through hole portions 4a and 4b, the core can be freely removed and the through hole molding with synthetic resin is easy.
Moreover, the reservoir portion 14 can be formed at the same time.
(2) 従つて合成樹脂の射出成型を利用して安価に
セルを製作することができる。(2) Therefore, cells can be manufactured at low cost using synthetic resin injection molding.
第１図は本考案の第一実施例を示す斜視図、第
２図は第１図のＢ−Ｂ断面図、第３図は本考案の
第二実施例を示す第２図同様の断面図、第４図は
従来のセルをホルダに取付けた状態を示す縦断面
図、第５図はセルを遠心分離機に取付けた状態を
示す縦断面図、第６図は先考案のセルを示す斜視
図、第７図は第６図のＡ−Ａ断面図である。
１……遠心塗抹用セル、２……基板、３……容
器筒、３ａ……横の部分、３ｂ……縦の部分、４
……通孔、４ａ，４ｂ……通孔部分、５……孔、
６……パツキング板、７……スライドガラス、８
……ホルダ、９……抑えばね、１０……遠心分離
機、１０ａ……蓋、１１……回転軸、１２……溜
り部、１３……補強リブ、１４……溜り部、１５
……蓋、１６……排出管。
Fig. 1 is a perspective view showing a first embodiment of the present invention, Fig. 2 is a sectional view taken along line BB in Fig. 1, and Fig. 3 is a sectional view similar to Fig. 2 showing a second embodiment of the invention. , Fig. 4 is a longitudinal cross-sectional view showing a conventional cell attached to a holder, Fig. 5 is a longitudinal cross-sectional view showing a cell attached to a centrifuge, and Fig. 6 is a perspective view showing the previously devised cell. FIG. 7 is a sectional view taken along the line AA in FIG. 6. 1... Cell for centrifugal smearing, 2... Substrate, 3... Container tube, 3a... Horizontal part, 3b... Vertical part, 4
...Through hole, 4a, 4b...Through hole part, 5...Hole,
6...Packing board, 7...Slide glass, 8
... Holder, 9 ... Pressure spring, 10 ... Centrifugal separator, 10a ... Lid, 11 ... Rotating shaft, 12 ... Reservoir, 13 ... Reinforcement rib, 14 ... Reservoir, 15
...Lid, 16...Discharge pipe.
Claims (1)
通孔４を基板２を貫通して反対側に開口させた遠
心塗抹用セルにおいて、通孔４を真直な横の通孔
部分４ａと真直な縦の通孔部分４ｂとを交叉させ
て形成すると共に、この交叉部の付近において、
横の通孔部分４ａより下方に位置する縦の通孔部
分４ｂの下端部を溜り部１４としたことを特徴と
する遠心塗抹用セル。 In a centrifugal smearing cell in which a container tube 3 is coupled to one side of a substrate 2 and a through hole 4 of the container tube 3 is opened to the opposite side through the substrate 2, the through hole 4 is connected to a straight horizontal hole portion. 4a and a straight vertical through hole portion 4b are formed to intersect with each other, and in the vicinity of this intersection,
A cell for centrifugal smearing characterized in that a lower end portion of a vertical through hole portion 4b located below the horizontal through hole portion 4a is a reservoir portion 14.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10008385U JPH0521003Y2 (en) | 1985-07-02 | 1985-07-02 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10008385U JPH0521003Y2 (en) | 1985-07-02 | 1985-07-02 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS629148U JPS629148U (en) | 1987-01-20 |
| JPH0521003Y2 true JPH0521003Y2 (en) | 1993-05-31 |
Family
ID=30969565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10008385U Expired - Lifetime JPH0521003Y2 (en) | 1985-07-02 | 1985-07-02 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0521003Y2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2526425Y2 (en) * | 1990-05-18 | 1997-02-19 | アラコ株式会社 | Accelerator interlock device |
| JP2008249565A (en) * | 2007-03-30 | 2008-10-16 | Mie Univ | Method for preparing cellular specimen |
-
1985
- 1985-07-02 JP JP10008385U patent/JPH0521003Y2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS629148U (en) | 1987-01-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5795489A (en) | Centrifugal filtration method | |
| US5738784A (en) | Device for separating a blood component from blood or plasma | |
| US3488768A (en) | Self-cleaning ultrafilter | |
| CN112517092B (en) | Centrifugal micro-fluidic chip and exosome extraction method | |
| US6719896B1 (en) | Fluid-filtration receptacle with user-variable semi-permeable drain assembly | |
| JPH0521003Y2 (en) | ||
| US6946079B1 (en) | Centrifugal filtration method for separating fibrin monomer from blood | |
| JP3715123B2 (en) | Blood filtration unit | |
| CN201163254Y (en) | Liquid based thin layer cytology inspection apparatus | |
| JPH0521002Y2 (en) | ||
| CN105132277B (en) | A kind of new separation of lymphocytes pipe | |
| JPH0516519Y2 (en) | ||
| JPH05843Y2 (en) | ||
| JPH0610281Y2 (en) | Centrifugal smear filter paper | |
| JPH0521004Y2 (en) | ||
| JP2001299730A (en) | Plasma collecting instrument | |
| JPH0230034Y2 (en) | ||
| JPH0438276Y2 (en) | ||
| CN216910630U (en) | Centrifugal cup | |
| CN2740272Y (en) | Dialysis container | |
| JPH034252U (en) | ||
| JP3765680B2 (en) | Blood filter | |
| JPH0436433Y2 (en) | ||
| JPH0641153Y2 (en) | Cell holder for centrifugal smear | |
| CN212758670U (en) | Device for preserving samples |