JPH05117126A - Cosmetic - Google Patents

Abstract

PURPOSE:To provide a skin cosmetic, capable of relieving skin inflammatory reaction and effective in improving skin conditions. CONSTITUTION:A cosmetic is obtained by blending a glutathione peroxidase in an amount of 0.0001-1wt.% based on the total amount of the cosmetic and copper or/and a selenium-containing substance so as to provide 0.00001-0.1wt.% expressed in terms of the amount of copper or/and selenium therein.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to cosmetics. Specifically, reduction of skin inflammatory reaction, skin condition (skin wrinkles,
It is intended to provide a cosmetic that is effective for improving firmness.

[0002]

2. Description of the Related Art Changes in the state of the skin are being clarified, including changes in the state of the epidermis and dermis, as well as biochemical changes in cells. The main cause of this change is
It is considered to be in the peroxidation reaction of the living body.

This peroxidation reaction is brought about by the action of various active oxygen produced by the reduction of oxygen in the living body. Active oxygen includes superoxide anion radical formed by one-electron reduction of oxygen, one-electron reduced hydrogen peroxide, one-electron reduced hydroxyl radical, singlet oxygen which is an excited oxygen molecule, etc. There is.

In general, active oxygen reacts with unsaturated fatty acids existing in biological membranes to produce lipid peroxide. It is considered that the lipid peroxide generated in the living body causes cell damage by reacting with a cell membrane or by binding to a membrane-bound enzyme or a membrane receptor protein. In addition, lipid peroxide is closely related to the arterial effect and is considered to cause aging.

In addition to such intrinsic factors, the skin is a tissue that is susceptible to external factors such as ultraviolet rays because it is in direct contact with the outside world, and the sebum on its surface is also affected. Contains a large amount of squalene and unsaturated fatty acids, which are substrates for lipid peroxides, and cosmetics applied to the skin also contain fats and oils, so lipid lipids are easily produced.

The relationship between the skin and lipid peroxide has been reported so far in female facial melasma, liver plaque, atopic dermatitis, sunburn and the like. In addition, squalene that has been peroxided using a xenon lamp has significant irritation to the skin,
It is known that the resulting inflammatory reaction is generally accompanied by pigmentation.

Since lipid peroxide has a bad influence on the skin as described above, reducing the lipid peroxide is not only for preventing aging of the skin, but also for keeping the skin healthy, youthful and fresh. It is also important in terms of maintenance.

Conventionally, vitamin E and its derivatives, vitamin C and the like have been used to suppress the production of lipid peroxides in the skin, and recently, enzymes such as superoxide dismutase and glutathione peroxidase are particularly effective. There are many reports that this is the case (as a review article, Vol. 11, No. 1, 1987).

By the way, it is known that selenium is involved in the activity expression of enzymes such as the above-mentioned superoxide dismutase or glutathione peroxidase (JW Forstrom et al., Biochemistry, 17: 2639, 1).
978 years).

Based on the results of such research, it is necessary to incorporate glutathione peroxidase into skin cosmetics for the purpose of improving and protecting the condition of the skin (see, for example, JP-A-64-4).
No. 7709), and incorporating selenium-containing algae into cosmetics for the purpose of suppressing skin aging (JP-A-63-3137).
08) is being conducted. Further, a hair cosmetic composition containing a selenium compound for the purpose of preventing dandruff is known (Japanese Patent Laid-Open No. 61-233608).

However, since conventionally known vitamins, enzymes and the like are extremely unstable, when incorporated into cosmetics, the effect of suppressing lipid peroxide production is lost over time.
Further, regarding the vitamins, although the above-mentioned effects are obtained, the peroxides already formed cannot be reduced.

[0012]

As described above, a compound having the effect of suppressing the production of peroxides, even if it can be stabilized, only suppresses the peroxide reaction and decomposes and eliminates it. It has no action, or even if it has the action of decomposing peroxide, it is extremely unstable, so it cannot be incorporated into cosmetics and inhibits the production of lipid peroxide, which is harmful to the skin. However, it was not possible to achieve the elimination of peroxide.

In order to overcome the above-mentioned drawbacks, the present invention aims to provide a skin cosmetic composition which reduces skin inflammatory reaction and is effective in improving the condition of the skin.

[0014]

Means for Solving the Problems The present inventor has conducted diligent research in view of these circumstances, and as a result, glutathione peroxidase was stabilized when a selenium-containing material was incorporated into a cosmetic composition, and the same was true for a copper-containing material. It was found that the above effect was obtained, and the present invention was completed.

That is, the present invention relates to the total amount of cosmetics,
0.0001 to 1% by weight of glutathione peroxidase and 0.0000 as a converted amount of copper and / or selenium
A cosmetic containing 1 to 0.1% by weight of a copper or / and selenium-containing material. Hereinafter, the present invention will be described in detail.

<1> Glutathione Peroxidase Glutathione peroxidase used in the present invention (hereinafter, referred to as
It is called GSH-Px. ) May have any origin, but is preferably an enzyme derived from an animal, an enzyme contained in a plant extract, or the like. As the animal-derived GSH-Px, a bovine erythrocyte-derived enzyme is particularly preferable, and it can be purchased from Toyobo Co., Ltd., Sigma.

These enzymes may be used alone or as a mixture, and 0.0001 to 1 relative to the total amount of the cosmetic.
It is preferably blended so as to be in a weight percentage. When GSH-Px derived from a plant is used, it may be blended as a plant extract. In that case, the amount of GSH-Px is blended within the above range.

When the content of GSH-Px is less than 0.0001% by weight, the peroxide cannot be decomposed sufficiently, the skin inflammatory reaction is reduced, and the effect of improving the skin condition cannot be expected. .. When the content exceeds 1% by weight, the effect of the present invention is not affected, but the rate of decomposing the peroxide reaches the ceiling, so it is desirable to mix within the above range.

<2> Copper or / and Selenium-Containing Material Used in the Present Invention The copper-containing material used in the present invention means an inorganic salt of copper such as copper (II) chloride and copper (II) sulfate, copper (II) acetate. , Copper citrate (I
Copper ions or nonionic copper such as salts of organic compounds such as I) and copper-containing animal and plant extracts such as molasses.

The selenium-containing material means an inorganic selenium compound such as selenate or selenite, an alkali metal selenate such as potassium selenite, an alkali metal selenite such as sodium selenite, Organic selenium compounds such as selenomethionine, selenocysteine, selenocystine, and selenocysteamine, and other organic selenium compounds such as salts thereof; ginseng extracts, rice extracts, selenium-containing animal and plant extracts such as algae extracts, and selenium ions or nonionic selenium.

The content of copper or selenium is 0.00001 to 0.1 in terms of copper or selenium, based on the total amount of the cosmetic.
It is compounded in the range of weight%. These copper- or selenium-containing materials may be used alone or as a mixture.

If the blending amount is less than this range, GSH-
The action of stabilizing Px becomes weak. When the amount exceeds this range, the stabilizing effect of GSH-Px reaches a peak, and further, the copper or selenium compound precipitates and the dispersibility deteriorates, which may cause coloration. Hereinafter, in the present specification, a copper or / and selenium-containing material is referred to as a stabilizer.

<3> Cosmetics of the Present Invention The cosmetics of the present invention are not particularly limited in dosage form, but in order to exert the effect of suppressing the generation of peroxides on the skin and erasing the same,
It is preferable that the selenium compound is percutaneously absorbed, and in this sense, a cosmetic that is applied to the skin and retains the components as it is is preferable to a cosmetic that is removed from the skin in a short time.

In addition to the above-mentioned essential components, the cosmetics of the present invention may contain components contained in ordinary cosmetics. Furthermore, components known to reduce skin inflammatory reaction and improve skin condition may be added.

Examples of such components include antioxidants (BHT, vitamin E, tocopherol, licorice extract, etc.), anti-inflammatory agents (licorice extract, sardine extract, mugwort extract, purple root extract, etc.), Moisturizer (marine life (seaweed,
(Kelp, squid, jellyfish, etc.) extract, toki extract, royal jelly, etc.), ultraviolet absorber (para-aminobenzoic acid and its derivatives, cinnamic acid esters, salicylic acid derivatives, etc.), ultraviolet scattering agent (fine particles TiO ₂ , Fine particles ZnO ₂
Etc.), other, vitamins A, C, F, H, B ₂ , B ₁₂ ,
Examples thereof include vitamins such as D ₂ , placenta extract, thymus extract, gastric mucosa mucin, and submandibular mucin.

[0026]

In the following, GSH-Px containing copper or selenium
Experimental results show that it has a stabilizing effect.

<Method> 0.02% GSH-Px (0.1
M phosphate buffer solution dissolved in pH 7.0) and a stabilizer having a predetermined concentration (0.1 M phosphate buffer solution pH 7.0)
A solution of 1: 1) and a mixture of
This was sealed and left in a thermostatic chamber at 40 ° C. for 1 month. still,
As a preservative, 0.1% of methyl paraoxybenzoate was added. GSH-Px activity before and after leaving for 1 month was measured,
The residual rate was calculated.

GSH-Px is an enzyme that eliminates hydrogen peroxide harmful to the living body by converting reduced glutathione into oxidized glutathione. Therefore, the enzyme activity is measured by measuring either reduction of reduced glutathione, increase of oxidized glutathione, or decrease of hydrogen peroxide.

Here, the activity of GSH-Px was determined by measuring the amount of oxidized glutathione produced using a simple and highly accurate HPLC analysis method. The principle of measurement is described in Lipid Peroxide Experimental Method (edited by Takashi Kaneda and Nobuo Ueda, Ito Dental Publishing Co., Ltd., p136, (1990)).

20 μl of the above sample was taken, 2.0 ml of 0.1 M phosphate buffer (pH 7.0) was added, and further 100 μl of 1.0% reduced glutathione and 1.0 ml of 0.01% hydrogen peroxide. Was added, the mixture was reacted at 20 ° C. for 10 minutes, 0.5 ml of 1N sulfuric acid was added to stop the reaction, and 10 μl of this reaction solution was analyzed by HPLC (high performance liquid chromatography) for the production of oxidized glutathione.
The measurement conditions of HPLC are as follows.

Column: ODS-bonded silica gel column (manufactured by Tosoh Corporation, 4.6 mmφ × 150 mm) Mobile phase: 0.02% phosphoric acid aqueous solution Flow rate: 1.0 to 2.0 ml / min Detection: Ultraviolet (210 nm) Absorption Incidentally, the enzyme activity was shown at pH 7.0, 20 ° C., with 1 unit being the enzyme activity that produces 5 μmol of oxidized glutathione per minute.

<Results> The results are shown in Table 1.

[0033]

[Table 1] From this result, it is GS
It can be seen that H-Px is stably retained. It is clear that this effect is more pronounced than vitamins.

[0034]

EXAMPLES Examples of the present invention will be described together with comparative examples.
The following compounding amounts are parts by weight.

[0035]

Examples 1 to 4 First, examples of lotion will be described.

<Manufacturing Method> The components of Table 2A dissolved at 50 ° C. were stirred and mixed, and the component of B previously mixed by stirring was added to solubilize the components. This was filled in a container to obtain a product.

[0037]

[Table 2]

<Confirmation of Effect> The effect of the lotion of this example was examined by a stability test, a skin inflammatory reaction reducing effect test and an actual use test (effect on skin wrinkles and firmness).

(1) Stability test GSH-Px contained in the cosmetics of Examples and Comparative Examples
In order to investigate the deactivation prevention effect of G., the activity of GSH-Px after standing at 40 ° C. for 1 month was measured, and the residual ratio to the activity immediately after production was determined. The activity of GSH-Px was measured in the same manner as above by dispersing 1 g of each lotion in 10 ml of water and using 1 ml of this as a sample. The results are shown in Table 3.

[0040]

[Table 3]

From these results, it was found that the copper- or selenium-containing substance or the lotion containing both of them can stably retain GSH-Px.

(2) Effect of Reducing Skin Inflammatory Reaction The effect of reducing inflammatory reaction was as follows: using the lotion of Examples 1 to 4 and the lotion of Comparative Example 1 left at 40 ° C. for 1 month, the inflammatory reaction by ultraviolet irradiation. Was used to evaluate the effect of reducing the inflammatory reaction.

Five irradiation sites of 1 × 1 cm were set on the forearms of 20 panelists, and the lotion of Examples 1 to 4 containing GSH-Px and a stabilizer was applied to each site by 0.05 m at each site.
The emulsion of Comparative Example 1 was applied uniformly to each of the 1 part.

After that, a Toshiba UV-B lamp (SE-30E) was set as an artificial light source so as to have an intensity of 0.7 mW / cm ^2, and 30 parts were applied to each part coated with the emulsion.
Seconds, 1 minute, 2 minutes, and 3 minutes of ultraviolet irradiation.

The day after the irradiation with ultraviolet rays, the degree of inflammation was evaluated according to the following criteria, and the results are shown in Table 4. ⊚: No inflammatory reaction is observed. ○: Slightly reddish. Δ: Erythema is clearly observed. X: Marked erythema is observed.

[0046]

[Table 4]

As is clear from this result, GSH-P
The area to which the lotion of Example containing x and the stabilizer was applied had almost no erythema even with a long irradiation time, or was slightly reddish, which could reduce the inflammatory reaction. I knew I could do it. It was also found that the effect can be exhibited even when the content of the stabilizer is about 0.0001%. Furthermore, it has been found that this effect is exhibited even after the cosmetic is left at 40 ° C. for a long period of time.

(3) Effect on wrinkles and firmness The effects of the product of the present invention on wrinkles and firmness were evaluated in Examples 1 to 4.
It evaluated by the actual use test using the lotion.

60 women aged 22 to 64 were divided into 3 groups of 20 people, and each group was given the lotion of Examples 1 and 4 and the lotion of Comparative Example 2 which were left at 40 ° C. for 1 month. The composition was used twice a day in the morning and in the evening for one month, and the effect on facial wrinkles and firmness was evaluated.

For wrinkles, a replica of the outer corner of the eye was prepared, the states before and after use were compared, and evaluation was made according to the following criteria. Improvement: Wrinkles were less noticeable or disappeared compared to before use. Non-improvement: Compared to before use, it is in the same state or rather, it has become noticeable.

On the other hand, for elasticity, the elasticity meter (Corte
x Technology, Dermaflex A)
Measure the elasticity of the cheek surface using, compare before and after use,
The following criteria evaluated.

Improvement: Elasticity increased compared to before use. Non-improved: Same or less elastic than before use. Table 5 shows the results of the above test and the results of the questionnaire of the panelists (skin condition).

[0053]

[Table 5] From this result, when actually used, GSH-
It is clear that the cosmetic containing Px and the stabilizer has an effect of improving the condition of the skin (wrinkles and firmness), and it was further found that this effect is stably maintained.

[0054]

[Embodiment 5] Next, an embodiment of a cream as a cosmetic of the present invention will be described. The oil phase component of Table 6A and the water phase component of B were dissolved at 70 ° C., and the component of B was gradually added to the component of A with stirring to obtain an oil-in-water emulsion.
This was cooled to 40 ° C, the component C was added, and the mixture was cooled to 30 ° C to obtain a cream.

[0055]

[Table 6] This cream retained the GSH-Px activity stably similarly to the lotion.

[0056]

According to the present invention, GSH-P is contained in cosmetics.
x can be held stably. As a result, it is possible to provide a cosmetic having an excellent effect of reducing the inflammatory reaction of the skin and improving the condition of the skin (for wrinkles and firmness).

Claims (1)

[Claims] 1. 0.0001 to 1 relative to the total amount of cosmetics
Wt% glutathione peroxidase and 0.00001 to 0.1 wt% as copper or / and selenium equivalent
Cosmetics containing the copper or / and selenium-containing material.