JPH0495013A - Beautifying and whitening cosmetic - Google Patents

Abstract

PURPOSE:To obtain a safe beautifying and whitening cosmetic, containing an extract separated from seaweeds belonging to the genus Chondrus of the family Gigartinaceae of red algae with water or a hydrophilic organic solvent and having strong beautifying and whitening action. CONSTITUTION:A beautifying and whitening cosmetic, containing an extract separated from seaweeds (Chondrus ocellatus Holm. f. crispus Okamura) belonging to the genus Chondrus of the family Gigartinaceae of red algae with water or a hydrophilic organic solvent (e.g. lower alcohols such as methanol, ethanol or isopropanol or ketones such as polyoxyethylene ethers or acetone) as an active ingredient and having stronger inhibitory action on tyrosinase activity than that of other seaweeds and safe for the skin for a long period.

Description

[Detailed description of the invention] [Industrial application field] The present invention relates to whitening cosmetics.

More specifically, the present invention relates to whitening cosmetics containing extracts of specific seaweed as an active ingredient.

[Conventional technology]

Conventionally, products that have the effect of inhibiting tyrosinase activity can be expected to have the effect of whitening the skin, and cosmetics containing various whitening substances that have this effect have been used.

As representative examples thereof, L-ascorbic acid or its fatty acid derivatives, phosphorylated ascorbic acid or its salts, etc. are well known.

However, ascorbic acid or its fatty acid derivative as a whitening agent has drawbacks such as being sensitive to heat and light, having poor stability in an aqueous solution, and causing decomposition and discoloration.

Furthermore, although phosphorylated ascorbic acid or its salts are excellent in stability, they have problems such as precipitation over time when added to lotions and the like.

Other skin whitening agents such as kojic acid and arbutin are known, but kojic acid causes coloration depending on the formulation, and conventional skin whitening agents have problems with stability and safety.

Examples of whitening cosmetics or melanin formation inhibitors containing seaweed extracts include JP-A-1-224308, JP-A-2-88592, and JP-A-2-124810.

JP-A-1-224308 discloses a cosmetic formulation consisting of a water-soluble extract containing phenolic compounds extracted from brown algae such as Hibamata, Konbu, Kurome, Arana, Hantawara, etc., and which does not have gel-forming ability. It is a liquid.

JP-A-2-88'592 discloses melanin formation inhibitors extracted from brown algae, green algae, and red algae, and focuses on the activity of brown algae. That is, it is an extract from seaweeds mainly including wakame, kelp, kajime, and obamoku.

Examples of red algae include Ogonanoori, Hirakusa, and Fukuronori.

JP-A-2-124810 discloses a cosmetic containing a hydrophilic solvent extract of a seaweed belonging to the brown algae, particularly belonging to the order Fucus or Laminata. Extracts of hijiki, wakame, and arame are exemplified.

[Problem to be solved by the invention]

Through experiments conducted by the present inventors, it was found that the tyrosinase activity inhibiting effect of the seaweed extract shown in the above-mentioned publication is not very strong, and that the effect varies greatly depending on the species of seaweed.

The purpose of the present invention is to search for a whitening substance that has a strong tyrosinase activity inhibiting effect, is safe for a long period of time, and is actually easy to use, and to provide a safe whitening cosmetic with a strong whitening effect using this substance. It is.

[Means to solve the problem]

The present inventors focused on the fact that the tyrosinase activity inhibition effect varies greatly depending on the type of seaweed, and determined the tyrosinase activity inhibition effect Al1 of various red algae.

9 times the volume of seaweed sodium phosphate buffer (10mM,
pH 6,8) was added and thoroughly homogenized. This was centrifuged to remove insoluble matter, and further filtered through a 0.45μ filter. The following experiment was conducted using this as a sample.

Sodium phosphate buffer (30mM, pH 6,8)
0.9 ml, 1.0 ml of the 1.66 m M tyrosine solution, and 1.0 ml of the sample solution were placed in a screw vial and heated in a constant temperature water bath at 37° C. for 5 minutes or more.

Add 0.1 ml of tyrosinase solution (manufactured by Sigma, derived from Matus rum, 914 Units/ml), keep warm in a constant temperature water bath at 37°C, and add 475n after 10 minutes.
Absorbance was measured at m.

In addition, as a control, pure water was added instead of the sample solution and the same measurements were carried out.

Tyrosinase activity inhibition rate (%) was calculated using the following formula.

Chicinase activity! lll rate (N)-[111-(A-P
)l/Ill]

Table 1 [Melanin production suppression test] 10% fetal bovine serum and a sample are added to Eagle MEM medium and used as a sample.

↓ Place the sample in a 6cm petri dish and add 1 x 10' B-16 cells.
Ce1l/0. Add 1 ml.

Cultured at 37°C with 15% CO2 Replace the specimen on the third day of culture.

Table 2 ↓ On the 6th day of culture, the cells were detached (physically detached as they were adherent) and centrifuged to observe the degree of whitening.

(Judgment criteria) Judgment based on whiteness core: No whitening +: Slight whitening ++: Obviously whitening +++: Strong whitening ++ indicates that there is an ability to suppress melanin production, and the higher the +, the more suppressive melanin production Demonstrate strong ability.

Table 2 shows the results of the melanin production inhibition test performed on the seaweed (Tochaka) extract of the present invention.

In the above-mentioned Japanese Patent Application Laid-open No. 2-88592, red algae such as Goonano-nori, Fukuro-nori, and Hirakusa are already known as red algae that have tyrosinase activity inhibiting action.
The names Ogonanori and Fukuronori do not exist as general names. If Ogona-nori is a mistake in Ogonori, its tyrosinase activity inhibition will be very low compared to the present invention, and if Fukuro-nori is a common name for Fukuro-funori, it will similarly be very effective compared to the present invention. It can be said that it is low.

However, as a red algae, Hiraksa actually exists;
It is clear from the above test results that there is no inhibition of this openness, so much so that it can almost be said to be inhibition of tyrosinase activity.

On the other hand, it has been revealed that Tochaka, a red alga of the present invention belonging to the genus Tsunomata of the family Suginoriaceae, has an extremely strong inhibitory effect on tyrosinase activity.

Examples of the hydrophilic organic solvent that is an extractant include lower alcohols such as methanol, ethanol, and isopropanol, and ketones such as polyoxyethylene ethers and acetone.

〔Example〕

The present invention will be explained in more detail with reference to examples below, but the present invention is not limited to the number of ships by these examples.

(Example 1) Lotion formulation Weight% ethanol
101.3 Butylene glycol
10 Tochaka extract (solid) 1 Water 79
(Comparative Example 1) Example 1 except that the Tochaca extract (solid substance) was replaced with water.

Sensory Evaluation 15 women and 8 men used the product for two months and completed a questionnaire.

The evaluation was based on a 5-level needle price scale, and was written as follows.

+5 The example is much better.

+4 Example is much better.

+3 Example is better.

+2 Example is slightly better.

+1 Example is slightly better.

0 There is no difference.

-1 Comparative example is slightly better.

-2 Comparative example is slightly better.

-3 Comparative example is better.

=4 Comparative example is considerably better.

-5 Comparative example is much better.

All comparative examples were conducted with Comparative Example 1.

This total value is shown in Table 3.

Table 3 As shown, seaweeds belonging to the genus Nomata of the family Suginoriaceae have a strong tyrosinase activity inhibitory effect, and their activity is stronger than other seaweeds in tyrosinase activity inhibition and panel tests. There was found.

〔Effect of the invention〕

According to the present invention, it has become clear that seaweed belonging to the genus Tsunomata of the family Suginoriaceae has a strong inhibitory effect on tyrosinase activity, and that this effect is stronger in tyrosinase activity inhibition and panel tests compared to other seaweeds. Ta.

Some of this seaweed is edible, so it may be safe for the skin over the long term.

Therefore, it has great utility as a raw material for cosmetics, both in terms of safety and effectiveness.

Claims (1)

[Claims] A whitening cosmetic containing an extract of seaweed belonging to the genus Tsunomata of the family Suginoriaceae using water or a hydrophilic organic solvent.