JPH0495012A - Beautifying and whitening cosmetic - Google Patents
Beautifying and whitening cosmeticInfo
- Publication number
- JPH0495012A JPH0495012A JP2208003A JP20800390A JPH0495012A JP H0495012 A JPH0495012 A JP H0495012A JP 2208003 A JP2208003 A JP 2208003A JP 20800390 A JP20800390 A JP 20800390A JP H0495012 A JPH0495012 A JP H0495012A
- Authority
- JP
- Japan
- Prior art keywords
- beautifying
- whitening
- seaweeds
- extract
- whitening cosmetic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000002087 whitening effect Effects 0.000 title claims abstract description 23
- 239000002537 cosmetic Substances 0.000 title claims abstract description 13
- 241001474374 Blennius Species 0.000 claims abstract description 28
- 239000000284 extract Substances 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003960 organic solvent Substances 0.000 claims abstract description 4
- 241000204357 Porites Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 24
- 102000003425 Tyrosinase Human genes 0.000 abstract description 18
- 108060008724 Tyrosinase Proteins 0.000 abstract description 18
- 241000199919 Phaeophyceae Species 0.000 abstract description 12
- 230000002401 inhibitory effect Effects 0.000 abstract description 9
- 241001261506 Undaria pinnatifida Species 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 241000243681 Eisenia bicyclis Species 0.000 abstract 1
- 241001216844 Ishige foliacea Species 0.000 abstract 1
- 241000631640 Ishige okamurae Species 0.000 abstract 1
- 241000631928 Ishigeaceae Species 0.000 abstract 1
- 241000264279 Sargassum fusiforme Species 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 10
- 239000000126 substance Substances 0.000 description 9
- 238000002835 absorbance Methods 0.000 description 8
- 239000007787 solid Substances 0.000 description 8
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 241001113425 Iridaceae Species 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 239000012488 sample solution Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000631636 Ishige Species 0.000 description 3
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000008099 melanin synthesis Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000012064 sodium phosphate buffer Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 150000000996 L-ascorbic acids Chemical class 0.000 description 2
- 241000121220 Tricholoma matsutake Species 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000007854 depigmenting agent Substances 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 2
- 229960004705 kojic acid Drugs 0.000 description 2
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 1
- RSWGJHLUYNHPMX-UHFFFAOYSA-N Abietic-Saeure Natural products C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C(O)=O RSWGJHLUYNHPMX-UHFFFAOYSA-N 0.000 description 1
- 241000512259 Ascophyllum nodosum Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 241000195480 Fucus Species 0.000 description 1
- 241001125831 Istiophoridae Species 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241001466453 Laminaria Species 0.000 description 1
- 230000010718 Oxidation Activity Effects 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- KHPCPRHQVVSZAH-HUOMCSJISA-N Rosin Natural products O(C/C=C/c1ccccc1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KHPCPRHQVVSZAH-HUOMCSJISA-N 0.000 description 1
- 241000195474 Sargassum Species 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 229940053200 antiepileptics fatty acid derivative Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229960000271 arbutin Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 229910000679 solder Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Cosmetics (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は特定の海藻の抽出物を有効成分とする美白化粧
料に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a whitening cosmetic containing an extract of a specific seaweed as an active ingredient.
従来、チロシナーゼ活性阻害効果をもったものは、肌を
白くするという効果が期待でき、この作用をもった各種
の美白作用を有する物質を配合した化粧料が使用されて
いる。Conventionally, products that have the effect of inhibiting tyrosinase activity can be expected to have the effect of whitening the skin, and cosmetics containing various whitening substances that have this effect have been used.
その代表的なものとしては、Ｌ−アスコルビン酸または
その脂肪酸誘導体、あるいはリン酸化アスコルビン酸ま
たはその塩などがよく知られている。As representative examples thereof, L-ascorbic acid or its fatty acid derivatives, phosphorylated ascorbic acid or its salts, etc. are well known.
しかしながら、美白剤物質としてのし一アスコルビン酸
またはその脂肪酸誘導体は、熱や光に弱く、水溶液中で
の安定性が悪く、分解や着色を起こすなどの欠点があっ
た。However, ascorbic acid or its fatty acid derivative as a skin whitening agent has drawbacks such as being sensitive to heat and light, having poor stability in an aqueous solution, and causing decomposition and discoloration.
またリン酸化アスコルビン酸またはその塩は安定性では
優れるものの、化粧水等に配合した場合に、経時′的に
沈殿を発生するなどの問題がある。Furthermore, although phosphorylated ascorbic acid or its salts have excellent stability, they have problems such as precipitation over time when added to lotions and the like.
その他、コウジ酸やアルブチン等が美白剤物質として知
られているが、コウジ酸は配合物によっては着色を起こ
すなど、従来の美白剤物質は安定性や安全性に問題があ
った。Other whitening agents such as kojic acid and arbutin are known, but kojic acid can cause coloration depending on the formulation, and conventional skin whitening agents have had problems with stability and safety.
海藻抽出物を配合する美白化粧料ないしメラニン形成阻
害物質としては、特開平１−
２２４３０８号公報、特開平２−８８５９２号公報、特
開平２−１２４８１０号公報がある。Examples of whitening cosmetics or melanin formation inhibitors containing seaweed extracts include JP-A-1-224308, JP-A-2-88592, and JP-A-2-124810.
特開平１−２２４３０８号公報は褐藻類ひばまた、こん
ぶ、くろめ、あらな、はんだわら等から抽出されたフェ
ノール性化合物を含有し、ゲル形成能を有しない水溶性
抽出物からなる化粧品配合液である。JP-A-1-224308 discloses a cosmetic formulation containing phenolic compounds extracted from brown algae Hibamata, kelp, Kurome, Arana, solder straw, etc., and consisting of a water-soluble extract without gel-forming ability. be.
特開平２−８８５９２号公報は、褐藻類、緑藻類、紅藻
類から抽出したメラニン形成阻害物質であるが、褐藻の
活性を中心としたものである。即ち、わかめ、こんぶ、
かしめ、おおばもくを中心とした海藻からの抽出物であ
る。JP-A-2-88592 discloses melanin formation inhibitors extracted from brown algae, green algae, and red algae, and focuses on the activity of brown algae. Namely, wakame, konbu,
It is an extract from seaweeds, mainly Kakime and Obamoku.
特開平２−１２４８１０号公報は、褐藻綱特にヒバマタ
目またはコンブ目に属する海藻の親水性溶媒抽出物を含
有する化粧料である。ひじき、わかめ、あらめの抽出物
が例示されている。JP-A-2-124810 discloses a cosmetic containing a hydrophilic solvent extract of a seaweed belonging to the Phaeophyceae, particularly the order Fucus or Laminata. Extracts of hijiki, wakame, and arame are exemplified.
本発明者らが実験したところ、前記公報に示されている
海藻抽出物のチロシナーゼ活性阻害作用はさほど強くな
く、また海藻の種によって大きくその作用が異なること
がわかった。Through experiments conducted by the present inventors, it was found that the tyrosinase activity inhibiting effect of the seaweed extract shown in the above-mentioned publication is not very strong, and that the effect varies greatly depending on the species of seaweed.
本発明の目的は、チロシナーゼ活性阻害作用か強く、且
つ安全てあり、実際に利用し易い美白剤物質を検索し、
これを用いて美白作用の強い安全な美白化粧料を提供す
ることである。The purpose of the present invention is to search for skin whitening substances that have a strong tyrosinase activity inhibitory effect, are safe, and are easy to use in practice.
The object of the present invention is to provide a safe whitening cosmetic with strong whitening effect using this.
本発明者らは、前記の課題を解決するため、後記のよう
な各種の海藻を用いて、そのチロシナー活性阻害率の測
定を試みた。その結果、驚くへきことに、同じ褐藻類に
属していても、前記公開公報に最も効果のあるものとし
て記載されているホンダワラ科のひしき、チガイソ科の
わかめ、コンブ科のあらめ等より、はるかにチロシナー
ゼ活性阻害効果の大きい海藻を見い出し本発明を完成し
た。In order to solve the above problems, the present inventors attempted to measure the inhibition rate of tyrosiner activity using various seaweeds as described below. As a result, surprisingly, even though they belong to the same brown algae, they are listed as the most effective in the published publication, such as sargassum, wakame, and laminaria, which are listed as the most effective. We have discovered a seaweed that has a far greater effect on inhibiting tyrosinase activity and have completed the present invention.
即ち本発明はイシゲ科の海藻の水または親水性有機溶媒
による抽出物を含む美白化粧料である。That is, the present invention is a skin-whitening cosmetic containing an extract of seaweed belonging to the family Polyaceae in water or a hydrophilic organic solvent.
本発明者らは、次のように褐藻類の海藻のチロシナーゼ
活性阻害を測定してみた。The present inventors measured inhibition of tyrosinase activity of brown algae and seaweed as follows.
海藻の９倍量のリン酸ナトリウム緩衝液（ｌｏｍＭ、
ｐ ＨＩ３．８ ）を加えて、充分ホモジナイズした。9 times the volume of seaweed sodium phosphate buffer (lomM,
pHI3.8) was added and thoroughly homogenized.
これを遠心分離し、不溶物を取り除き、さらに０．４５
μのフィルターてｉ濾過した。これをサンプルとして以
下の実験を行った。This was centrifuged to remove insoluble matter, and then 0.45
It was filtered using a μ filter. The following experiment was conducted using this as a sample.
リン酸ナトリウム緩衝液（３０ｍＭ、 ｐＨ６，８）（
１，９ｍｌ、１．８ＢｍＭ チロシン 溶液１．０ｍ
ｌ、試料溶液１．０ｍｌをスクリューバイアルにとり、
３７℃恒温水槽中で５分以上加温した。Sodium phosphate buffer (30mM, pH 6,8) (
1.9ml, 1.8BmM tyrosine solution 1.0m
l. Take 1.0ml of the sample solution into a screw vial,
It was heated for 5 minutes or more in a constant temperature water bath at 37°C.
チロシナーゼ溶液（Ｓｉｇｍａ社製、マツシュルーム由
来、９１４ Ｕｎｉｔｓ ／ ｍｌ ） ［１，１ｍｌ
を加え、３７℃恒温水槽中で保温し、１０分後に４７５
ｎｍで吸光度を測定した。Tyrosinase solution (manufactured by Sigma, derived from pine mushroom, 914 Units/ml) [1.1ml
was added, kept warm in a constant temperature water bath at 37℃, and after 10 minutes, 475℃ was added.
Absorbance was measured at nm.
又、対照として、試料溶液のがわりに純水を加え同様に
測定した。In addition, as a control, pure water was added instead of the sample solution and measurements were made in the same manner.
チロシナーゼａＮＩＨ＊（％）−１Ｂ−（Ａ−Ｐ）ｌ／
ＢＸＩＯＩ）但し、Ａ；試験検体の吸光度
Ｂ：対照の吸光度
Ｐ：試験検体の着色による吸光度
（３倍希釈）
結果は第１表のごとくになった。Tyrosinase aNIH*(%)-1B-(A-P)l/
BXIOI) However, A: Absorbance of test specimen B: Absorbance of control P: Absorbance of colored test specimen (3-fold dilution) The results are as shown in Table 1.
第 １ 表
このような結果となり、先行特許に示されているように
褐藻類のすべてに効果かあるわけてはなく、また先行特
許の最も効果のあるものを記載すべき実施例のものも、
弱い作用のものが多かった。Table 1 The results are as shown above, and as shown in the prior patent, it is not effective for all brown algae, and the most effective example of the prior patent should also be described.
Many had weak effects.
本発明者らが行った実験によれば、一般にチロシナーゼ
活性阻害かあるといわれているひしき、あらめ、かじめ
等より、さらにイシゲ科の海藻はチロシナーゼ活性阻害
が強いことが判明した。According to experiments conducted by the present inventors, it was found that seaweeds belonging to the family Iridaceae have a stronger inhibition of tyrosinase activity than seaweeds such as Hishiki, Arame, and Kajime, which are generally said to inhibit tyrosinase activity.
さらに、かしめ、あらめは他の海藻に比べれば、若干チ
ロシナーゼ活性阻害はあるが、以下の実験によってイシ
ゲ科の海藻は千ロジンかメラニンに変化する各段階に作
用し、より有効であることか確認できた。Furthermore, although Kashima and Arame inhibit tyrosinase activity slightly compared to other seaweeds, the following experiment shows that seaweeds of the family Ishigamiaceae are more effective as they act on each stage of the transformation into rosin or melanin. It could be confirmed.
次のように気質にドーパを用いて、チロシナーゼのドー
パ酸化活性に対する阻害について試験した。即ちリン酸
ナトリウム緩衝液（３０ｍＭ、ｐＨ６４） ０．９ ｍ
ｌ １．６６ｍＭドーパ溶液１ 、０ ｍｌ ｓ試料溶
液１．Ｏｍｌをスクリューバイアルにとり３７℃恒温水
槽中で５分以上加温した。チロシナーゼ溶液（Ｓｉｇｍ
ａ製、マツシュルーム由来、９１４ Ｕｎｉｔｓ ／ｍ
ｌ） ０．１ ｍｌを加え、３７℃恒温水槽中で保温し
、２分後に４７５ｎｍで吸光度を測定した。又対照とし
て試料溶液のかわりに純水を加え同様に測定した。Dopa was used to test for inhibition of tyrosinase oxidation activity of dopa as follows. Sodium phosphate buffer (30mM, pH 64) 0.9m
l 1.66mM dopa solution 1, 0 ml s sample solution 1. Oml was placed in a screw vial and heated in a constant temperature water bath at 37° C. for 5 minutes or more. Tyrosinase solution (Sigma
Made by a, derived from pine mushrooms, 914 Units/m
l) 0.1 ml was added, kept warm in a constant temperature water bath at 37°C, and absorbance was measured at 475 nm after 2 minutes. As a control, pure water was added instead of the sample solution and the same measurements were performed.
チロシナーゼ活性阻害率（％）−１Ｂ−（Ａ−Ｐ）ｌ／
ＢＸ１００但し、Ａ：試験検体の吸光度
Ｂ：対照の吸光度
Ｐ：試験検体の着色による吸光度
（３倍希釈）
結果は第２表のようになった。Tyrosinase activity inhibition rate (%) -1B-(A-P)l/
BX100 However, A: Absorbance of test specimen B: Absorbance of control P: Absorbance of colored test specimen (3-fold dilution) The results are as shown in Table 2.
第 ２ 表
〔メラニン生成抑制試験〕
Ｅａｇｌｅ ＭＥＭ培地に牛胎児血清１０％および試
料を加え、検体とする。Table 2 [Melanin production suppression test] 10% fetal bovine serum and a sample were added to Eagle MEM medium to serve as a sample.
↓
６ｃｍシャーレに検体を入れ、Ｂ−１６細胞１×１０５
Ｃｅｌｌ／ ０． １ ｍ ｌを加える。↓ Place the sample in a 6cm petri dish and add 1 x 105 B-16 cells.
Cell/0. Add 1 ml.
↓５％ＣＯ□、３７℃で培養 培養３日目に検体を交換する。↓Culture at 37℃ with 5% CO□ Replace the specimen on the third day of culture.
第３表
↓
培養６日目に細胞を剥離しく付着性であるので物理的に
剥離）遠心分離して、白色化度を観察する。Table 3 ↓ On the 6th day of culture, the cells are detached (physically detached as they are adherent) and centrifuged to observe the degree of whitening.
（判定基準）白色度による判定
ｃｏｒｅ
−：白色化なし
＋：わずかに白色化
＋＋：明らかに白色化
＋＋十二強い白色化
＋＋はメラニン生成抑制能力があることを表わし、十が
多い程、メラニン生成抑制能力が強いことを示す。(Judgment criteria) Judgment based on whiteness core -: No whitening +: Slight whitening ++: Obviously whitening ++ 12 Strong whitening ++ indicates the ability to suppress melanin production; Indicates strong ability to suppress production.
本発明の海藻（いしげ、いろろ）抽出物について、前記
メラニン生成抑制試験を行った結果を第３表に示す。Table 3 shows the results of the melanin production inhibition test performed on the seaweed (Ishige, Iroro) extract of the present invention.
いしげ（Ｉｓｈｉｇｅ ｏｋａｇ＋ｕｒａｉ Ｙｅｎｄ
ｏ）、いろろ（Ｉｓｈｉｇｅ ５ｉｎｉｃｏｌａ Ｃｈ
ｉｈａｒａ）等のイシゲ科の海藻が他の海藻、特に褐藻
類の海藻と比較して有用なことがわかった。Ishige okag+urai Yend
o), Iroro (Ishige 5inicola Ch)
It has been found that seaweeds of the family Ishigaceae, such as A. ihara), are more useful than other seaweeds, especially seaweeds of the brown algae family.
いしげ科の海藻は、一部で食用にされている事実から、
安全性に関しても問題ないことは勿論である。Due to the fact that some seaweeds of the Shigeidae family are eaten as food,
Of course, there are no problems with safety.
以下に実施例により、本発明を更に具体的に説明するが
、本発明はこの実施例により同等限定されるものでない
ことは勿論である。The present invention will be explained in more detail below with reference to Examples, but it goes without saying that the present invention is not limited to the same extent by these Examples.
（実施例１）
化粧水処方 重量％エタノール
１０１．３ブチレングリコール
１０
いしげ抽出物（固形物） １
水 ７
９（実施例２）
実施例１において、いしげ抽出物（固形物）をいろろ抽
出物（固形物）に置き換えたもの。(Example 1) Lotion formulation Weight% ethanol
101.3 Butylene glycol
10 Shigege extract (solid) 1 Water 7
9 (Example 2) Example 1 except that the Shigeki extract (solid substance) was replaced with the Iroro extract (solid substance).
（比較例１）
実施例１において、いしげ抽出物（固形物）を水に置き
換えたもの。(Comparative Example 1) Example 1 except that the Shigege extract (solid substance) was replaced with water.
（比較例２）
実施例１において、いしげ抽出物（固形物）をあらめ抽
出物（固形物）に置き換えたもの。(Comparative Example 2) Example 1 except that the Shigege extract (solid substance) was replaced with Arame extract (solid substance).
（比較例３）
実施例１において、いしげ抽出物（固形物）をかじき抽
出物（固形物）に置き換えたもの。(Comparative Example 3) Example 1 except that the Shigeki extract (solid substance) was replaced with the Marlin extract (solid substance).
官能評価
女性２０名、男性９名に対して、２ケ月間使用し、アン
ケートを取った。Sensory evaluation 20 women and 9 men used the product for two months and completed a questionnaire.
評価は５段階評価で、以下のように記載してもらった。The evaluation was on a five-point scale and was written as follows.
＋５ 実施例の方か非常によい。+5 The example is very good.
＋４ 実施例の方かかなりよい。+4 The example is considerably better.
＋３ 実施例の方かよい。+3 The example is better.
＋２ 実施例の方かややよい。+2 The example is slightly better.
＋１ 実施例の方が若干よい。+1 Example is slightly better.
０ 差がない。0 There is no difference.
１ 比較例の方が若干よい。1 Comparative example is slightly better.
２ 比較例の方がややよい。2 Comparative example is slightly better.
３ 比較例の方がよい。3 Comparative example is better.
４ 比較例の方がかなりよい。4 Comparative example is much better.
５ 比較例の方か非常によい。5 Comparative example is very good.
比較例はすべて、比較例１と行った。All comparative examples were conducted with Comparative Example 1.
この合計値を第４表に示す。This total value is shown in Table 4.
このように、イシゲ科の海藻はチロシナーゼ活性阻害効
果が強く、チロシーゼ活性阻害効果やパネルテストにお
いても、他の海藻に比較して、その作用が強いことが判
明した。In this way, seaweeds belonging to the family Iridaceae have a strong tyrosinase activity inhibitory effect, and in tyrosinase activity inhibitory effects and panel tests, it has been found that this effect is stronger than that of other seaweeds.
本発明によって、褐藻類の海藻すべてがチロシナーゼ活
性阻害効果即ち美白作用が強いわけてはなく、特にイシ
ゲ科の海藻が他の海藻特に他の褐藻類に比較してチロシ
ナーゼ活性阻害効果即ち美白作用が強く、パネルテスト
においても、その作用が実証された。According to the present invention, not all brown algae have a strong tyrosinase activity inhibiting effect, that is, a whitening effect, and in particular, seaweeds of the family Iridaceae have a tyrosinase activity inhibiting effect, that is, a whitening effect, compared to other seaweeds, especially other brown algae. Its effectiveness was strongly demonstrated in panel tests.
イシゲ科の海藻は、一部で食用に供されていることから
安全性については問題なく、美白化粧料として、安全性
の面からも、その美白効果の面からも十分に利用価値が
あることが明白となった。Since some seaweeds of the family Iridaceae are edible, there are no safety issues, and they have sufficient utility as skin-whitening cosmetics, both from the safety perspective and from the whitening effect. became clear.
出願人代理人 藤 本 博 光−９：Applicant's agent Hiroshi Fujimoto-9:
Claims (1)
を含む美白化粧料。A skin whitening cosmetic containing an extract of seaweed belonging to the family Porites in water or a hydrophilic organic solvent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2208003A JP2970767B2 (en) | 1990-08-08 | 1990-08-08 | Whitening cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2208003A JP2970767B2 (en) | 1990-08-08 | 1990-08-08 | Whitening cosmetics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0495012A true JPH0495012A (en) | 1992-03-27 |
| JP2970767B2 JP2970767B2 (en) | 1999-11-02 |
Family
ID=16549061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2208003A Expired - Lifetime JP2970767B2 (en) | 1990-08-08 | 1990-08-08 | Whitening cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2970767B2 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030015536A (en) * | 2001-08-16 | 2003-02-25 | 벤트리 주식회사 | Whitening cosmetics containing extracts from Ishige sinicola |
| KR101132572B1 (en) * | 2009-04-06 | 2012-04-05 | 재단법인 제주테크노파크 | Skin Whitening Composition |
| KR101365822B1 (en) * | 2012-02-10 | 2014-02-20 | 제주대학교 산학협력단 | Skin whitening composition comprising octaphlorethol A compound |
| JP2014097959A (en) * | 2012-11-15 | 2014-05-29 | Mikimoto Pharmaceut Co Ltd | Claudin production promoter, occludin production promoter, and enhancer for function of tight junction |
| JP2014101309A (en) * | 2012-11-20 | 2014-06-05 | Mikimoto Pharmaceut Co Ltd | Cystatin a production promoter, skin barrier increasing agent, skin keratinization function increasing agent, and skin roughness preventing agent |
| JP2014133708A (en) * | 2013-01-09 | 2014-07-24 | Mikimoto Pharmaceut Co Ltd | Dendrite growth inhibitor |
-
1990
- 1990-08-08 JP JP2208003A patent/JP2970767B2/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030015536A (en) * | 2001-08-16 | 2003-02-25 | 벤트리 주식회사 | Whitening cosmetics containing extracts from Ishige sinicola |
| KR101132572B1 (en) * | 2009-04-06 | 2012-04-05 | 재단법인 제주테크노파크 | Skin Whitening Composition |
| KR101365822B1 (en) * | 2012-02-10 | 2014-02-20 | 제주대학교 산학협력단 | Skin whitening composition comprising octaphlorethol A compound |
| JP2014097959A (en) * | 2012-11-15 | 2014-05-29 | Mikimoto Pharmaceut Co Ltd | Claudin production promoter, occludin production promoter, and enhancer for function of tight junction |
| JP2014101309A (en) * | 2012-11-20 | 2014-06-05 | Mikimoto Pharmaceut Co Ltd | Cystatin a production promoter, skin barrier increasing agent, skin keratinization function increasing agent, and skin roughness preventing agent |
| JP2014133708A (en) * | 2013-01-09 | 2014-07-24 | Mikimoto Pharmaceut Co Ltd | Dendrite growth inhibitor |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2970767B2 (en) | 1999-11-02 |
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