JPH0491009A - Skin cosmetic - Google Patents

Abstract

PURPOSE:To obtain a skin cosmetic, having excellent preventing effects on skin roughening and aging, and further beautifying and whitening effects, and stable to preservation for a long period without any side effects and skin irritation by blending a beautifying and whitening agent with a deproteinized blood serum substance. CONSTITUTION:A skin cosmetic is obtained by containing 0.01-30wt.% one or two or more beautifying and whitening agents selected from ascorbic acid derivatives, hydroquinone derivatives, pyrones and bovine placental extracts and a deproteinized blood serum substance in an amount of 0.0001-0.5wt.% expressed in terms of dry solid substance, further suitably blending coloring matters, perfumes, preservatives, surfactants, pigments, antioxidants, etc., therewith and preparing the skin cosmetic according to a conventional method. The aforementioned cosmetic can be prepared in the form of formulations such as cream, milky lotion, toilet water, pack or powder. The beautifying and whitening agent is well known and the deproteinized blood serum substance is obtained by a method for electrolyzing a liquid prepared from blood serum of a calf, etc., under acidic conditions, etc.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention contains one or more selected from ascorbic acid derivatives, hydroquinone derivatives, pyrones, and bovine placenta extract, and a serum deproteinized product. In more detail, the skin cosmetics have no undesirable side effects or skin irritation on the human body, are stable even after long-term storage, and have excellent anti-aging effects, anti-aging effects on the skin, and excellent whitening effects. The present invention relates to skin cosmetics that can simultaneously express and impart the following.

[Prior art and invention or problem to be solved] Conventionally,
Skin cosmetics aimed at preventing and improving skin roughness have been formulated with various raw materials extracted from natural products such as proteins, polysaccharides, extracted extracts, and natural polymers, but the effects of preventing and improving skin roughness have not been found. However, the development of better skin cosmetics was long awaited.

As a result of intensive research to develop such skin cosmetics, the present inventors discovered an ascorbic acid derivative.

Skin cosmetics containing skin whitening agents such as hydroquinone derivatives, pyrones, and bovine placenta extract, as well as bovine serum deproteinized products, not only have excellent effects on preventing skin roughness and preventing skin aging, It was discovered that the effect was significantly improved, and the present invention was completed.

[Means to solve the problem]

The present invention is a skin cosmetic containing one or more selected from ascorbic acid derivatives, hydroquinone derivatives, pyrones, and bovine placenta extract, and bovine serum deproteinized product.

The ascorbic acid derivatives, hydroquinone derivatives, pyrones, and bovine placenta extract used in the present invention are known whitening agents.

Ascorbic acid derivatives include L-ascorbic acid monostearate, L-ascorbic acid monopalmitate, L-ascorbic acid distearate, L-ascorbic acid dipalmitate, L-ascorbic acid dioleate,
Oil-soluble ascorbic acid derivatives such as L-ascorbic acid tristearate, L-ascorbic acid tripalmitate, and L-ascorbic acid triolate, L-ascorbic acid, L-ascorbic acid phosphate, and L-ascorbic acid sulfate. , 6-0-acylascorbic acid phosphate, etc. Sodium salts Potassium salts 1 Magnesium salts, calcium salts, barium salts, ammonium salts, monoethanolamine salts, jetanolamine salts, triethanolamine salts, monoisopropanolamine Examples include, but are not limited to, salts, diisopropanolamine salts, triisopropanolamine salts, and water-soluble ascorbic acid derivatives such as 3-0-isopropyl-L-ascorbic acid.

Examples of hydroquinone derivatives include condensates of hydroquinone and sugar, and condensates of alkylhydroquinone and sugar, in which one alkyl group having 1 to 4 carbon atoms is introduced into hydroquinone, such as arbutin.

Examples of the pyrones include kojic acid and meconic acid. Examples of bovine placenta extract include water-soluble placenta extract (manufactured by Chirei).

One or more of these whitening agents may be blended. The content thereof is preferably 0.01 to 30% by weight, more preferably 0.01 to 30% by weight, based on the total amount of prescription ingredients of the cosmetic.
It is within the range of 1 to 10% by weight.

If it is less than 0.01% by weight, it is difficult to obtain an effect, and if it exceeds 30% by weight, the storage stability of the product will be poor, which is not preferable.

The serum protein-free product used in the present invention can be obtained, for example, by the following method.

That is, the blood of a raw material such as a larva is frozen, activated, thawed, and the resulting liquid is heated under acidic conditions and electrolyzed by applying an electric current, and the resulting precipitate is removed to obtain the resulting liquid. Concentrate to obtain an extract.

Another method is to remove cellulose from the blood of larvae, etc., which is the raw material, add acetone, and then add acetone:ether (11
) and dry after centrifugation. Add distilled water to the obtained dried product, add tribson, and ferment at pH 8 and 37°C. After fermentation, cellophane dialysis is performed and concentrated to obtain an extract.

The resulting extract is a yellowish, transparent liquid containing 4-5% solids.

In addition, these extracts include Solcoseryl (manufactured by Solcobasel) or Nissur 71 (manufactured by BOTTGER).
It can be purchased as.

Its content, as a dry solid amount, is in the range of 0.0001 to 0.5% by weight, preferably 0.001 to 0.1% by weight, based on the total amount of prescription ingredients of the cosmetic. 0.0
The effect is not exhibited when the amount of M is less than 0.5%.
Exceeding this percentage by weight is not preferable because the storage stability of the product is poor.

In addition to the above-mentioned raw materials, the skin cosmetic of the present invention also contains pigments.

Flavors, preservatives, surfactants, pigments, antioxidants, and the like may be added as appropriate within the scope of achieving the object of the present invention.

The dosage form of the skin cosmetic of the present invention includes cream emulsion, lotion, bag, powder, and the like.

The skin cosmetic of the present invention, for example, in the case of a milky lotion, can be produced by a conventional method in which an oil phase and an aqueous phase are respectively heated and dissolved, emulsified and dispersed, and then cooled.

〔Example〕

Examples will be described below. Note that the percentages shown in the examples are percentages by weight. The methods for measuring the turnover rate of the stratum corneum, the test method for measuring the rough skin improvement effect, the test method for measuring the corneum improvement effect, the sensory test, and the skin color brightness recovery test method described in the Examples are as follows.

(1) Method for measuring the turnover rate of the stratum corneum An ointment containing 5% by weight of the fluorescent dye danzyl chloride in white petrolatum is made and applied to the skin of the subject's forearm with a closed lid for 24 hours. Osmotic bonding of luchlorite. Thereafter, the test sample was applied to the same area twice a day (morning and evening), and the fluorescence of danzyl chloride was examined every day.The number of days until the fluorescence disappeared was defined as the turnover rate of the skin stratum corneum.

Note that the normal turnover rate of the stratum corneum of the skin is 14
It lasts for ~16 days, or around 18 days in older skin. On the other hand, if the anti-aging effect appears, the time will be shortened to around 12 days.

(2) Test method for measuring the effect of improving rough skin The effect of continuous application for 4 weeks was investigated on 20 middle-aged and elderly subjects who had rough skin on their lower legs. Approximately 1 g of the sample was applied to the test site of the left lower leg of the subject twice a day, and the condition of the skin before and after the test was judged according to the following criteria.

No sample was applied to the right lower leg, which served as a control.

Judgment criteria for skin dryness: Normal ±: Slight dryness, no flaking +: Mild dryness, flaking + 10: Moderate dryness, flaking + 10: Marked dryness, flaking Compare the judgment results of the test site and control site before and after the test. , if the skin dryness level has improved by two or more levels (e.g. 10--2)
10→±) was considered ``effective,'' one level of improvement was considered ``slightly effective,'' and no change was considered ``ineffective.'' The test result is "valid".

It is indicated by the number of subjects who found it "somewhat effective."

(3) Test method for measuring keratin improvement effect (increase in anti-peeling properties of keratinocytes) A scotch tape (Tiban mending tape) is adhered to the skin of the test subject before and after the above-mentioned rough skin improvement measurement test, and this is peeled off. The condition of the keratinocytes that adhered to the tape was examined in detail using a scanning electron microscope, and the anti-exfoliation properties of the skin keratinocytes were determined according to the following criteria.

Judgment criteria for keratin improving effect (increased anti-exfoliation property of keratinocytes) score of 1 scale was not observed 2. Small scale scattered〃 3 Small to medium scale remarkable evaluation points 4 Large scale neck lesions judgment: 1 point for “valid” if the difference between the evaluation point of the test site after 4 weeks of continuous application and that of the control site is 2 points or more A score of 0 points was defined as "slightly effective", and a score of 0 was defined as "invalid". The test results are shown in terms of the number of subjects who found the drug to be "effective" or "slightly effective."

(4) Sensory test (skin beautification effect test) Samples were applied twice a day (morning and evening) for three consecutive months to 20 female subjects (35 to 55 years old) who complained of rough skin, fine wrinkles, dry skin, etc.
The effects were evaluated after 2.3 months. The test results were ``skin moisturized'' for each item of skin wettability, smoothness, and elasticity.

The number of people who answered ``My skin became smoother and my skin became taut'' was shown.

It also shows the period of time it takes to see an effect.

(5) Skin color brightness recovery test method: The back skin of 20 subjects was irradiated with ultraviolet rays in the UV-B region twice the minimum amount of erythema, and the reference brightness of each skin was determined by setting sample application areas and non-application areas. (Vo value, Vo' value) were measured. Subsequently, apply 3 samples to the application site twice a day.
After 3, 8 and 13 weeks, the skin brightness (Vn value, Vn' value) of the applied area and non-applied area was measured, and the recovery of skin color was evaluated according to the following criteria.

In addition, the brightness of the skin (Munsell color system V value) was calculated from the x, y, and z values obtained by measurement using a high-speed spectrocolorimeter. Moreover, the evaluation was shown as the average value of the evaluation scores of 20 subjects after 13 weeks.

Examples 1-9. Comparative Examples 1 to 6 (Skin Cream) A whitening agent and a serum deproteinized product were blended in the following composition,
Skin creams were prepared and the tests described above were conducted on each.

(1) Composition (2) Preparation method Dissolve (A) uniformly at 70°C and (B) at 50°C, and pour (B) into (A) while stirring (A) to emulsify. After dispersion, the mixture is prepared by cooling to a temperature of 30°C while stirring.

(3) Characteristics Table 1 shows the results of various tests conducted on each skin cream. As shown in Table 1, Comparative Examples 1 to 3 in which a whitening agent was blended alone. Comparative Examples 4 to 5 in which serum protein-removed products were blended alone. The skin cream of Comparative Example 6, which did not contain either of these, did not have sufficient effects in terms of various properties. On the other hand, the skin creams containing the skin whitening agents and serum-removed proteins of Examples 1 to 9 of the present invention showed remarkable effects in various properties, and the sensory tests showed that the skin creams containing the skin whitening agents and serum deproteinized substances of Examples 1 to 9 of the present invention showed remarkable effects in various properties, and the sensory tests showed that after applying the sample
It showed excellent skin beautification effects within 2 months.

Example 10 (Emulsion) After dispersing, add component (C) and heat to 30° while stirring.
Prepare by cooling to C.

(3) Characteristics The obtained emulsion showed good results in the various tests mentioned above.

Example 11 (Lotion) (1) Composition (2) Preparation method Dissolve (A) uniformly at 70°C and (B) at 50''C, and dissolve (B) while stirring (A). Inject into A) and mix and dissolve emulsified component (2) Preparation method (A) and (B) at room temperature, and (B)
Prepare by adding (A) to and stirring.

(3) Characteristics The obtained lotion showed good results in the various tests mentioned above.

〔Effect of the invention〕

As mentioned above, it is clear that the skin cosmetic of the present invention synergistically increases the skin roughness prevention effect, skin aging prevention effect, and whitening effect by blending the whitening agent and the serum protein-removed product. be.

Claims (1)

[Claims] A skin cosmetic comprising one or more selected from ascorbic acid derivatives, hydroquinone derivatives, pyrones, and bovine placenta extract, and a serum protein-removed product.