JPH045440B2 - - Google Patents
Info
- Publication number
- JPH045440B2 JPH045440B2 JP58052772A JP5277283A JPH045440B2 JP H045440 B2 JPH045440 B2 JP H045440B2 JP 58052772 A JP58052772 A JP 58052772A JP 5277283 A JP5277283 A JP 5277283A JP H045440 B2 JPH045440 B2 JP H045440B2
- Authority
- JP
- Japan
- Prior art keywords
- cell
- cells
- potential
- electrode
- peak
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 claims description 22
- 239000006285 cell suspension Substances 0.000 claims description 11
- 239000000725 suspension Substances 0.000 claims description 7
- MWVTWFVJZLCBMC-UHFFFAOYSA-N 4,4'-bipyridine Chemical compound C1=NC=CC(C=2C=CN=CC=2)=C1 MWVTWFVJZLCBMC-UHFFFAOYSA-N 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 51
- 241000894006 Bacteria Species 0.000 description 14
- 238000005259 measurement Methods 0.000 description 13
- 244000005700 microbiome Species 0.000 description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000002484 cyclic voltammetry Methods 0.000 description 3
- 238000001736 differential pulse polarography Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000004832 voltammetry Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000186840 Lactobacillus fermentum Species 0.000 description 2
- 241000192130 Leuconostoc mesenteroides Species 0.000 description 2
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 description 2
- 238000000086 alternating current polarography Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000003411 electrode reaction Methods 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229940012969 lactobacillus fermentum Drugs 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000000067 square-wave polarography Methods 0.000 description 2
- 238000010408 sweeping Methods 0.000 description 2
- 238000001075 voltammogram Methods 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 238000010953 Ames test Methods 0.000 description 1
- 231100000039 Ames test Toxicity 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000227653 Lycopersicon Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000001903 differential pulse voltammetry Methods 0.000 description 1
- 238000001318 differential pulse voltammogram Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000015193 tomato juice Nutrition 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 239000007222 ypd medium Substances 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58052772A JPS59179097A (ja) | 1983-03-30 | 1983-03-30 | 細胞の電気化学的活性測定方法 |
| US06/546,101 US4528270A (en) | 1982-11-02 | 1983-10-27 | Electrochemical method for detection and classification of microbial cell |
| DE19833339408 DE3339408A1 (de) | 1982-11-02 | 1983-10-29 | Verfahren zur elektrochemischen feststellung und klassifizierung mikrobieller zellen |
| GB08329095A GB2131954B (en) | 1982-11-02 | 1983-11-01 | Electrochemical method for detection and classification of microbial cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58052772A JPS59179097A (ja) | 1983-03-30 | 1983-03-30 | 細胞の電気化学的活性測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59179097A JPS59179097A (ja) | 1984-10-11 |
| JPH045440B2 true JPH045440B2 (de) | 1992-01-31 |
Family
ID=12924154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58052772A Granted JPS59179097A (ja) | 1982-11-02 | 1983-03-30 | 細胞の電気化学的活性測定方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS59179097A (de) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07184686A (ja) * | 1993-12-28 | 1995-07-25 | Nec Corp | 細胞活性測定方法 |
| GB201020619D0 (en) * | 2010-12-06 | 2011-01-19 | Syngenta Ltd | Pathogen sensor |
-
1983
- 1983-03-30 JP JP58052772A patent/JPS59179097A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS59179097A (ja) | 1984-10-11 |
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