JPH04311322A - Method for cultivating plant - Google Patents
Method for cultivating plantInfo
- Publication number
- JPH04311322A JPH04311322A JP9984091A JP9984091A JPH04311322A JP H04311322 A JPH04311322 A JP H04311322A JP 9984091 A JP9984091 A JP 9984091A JP 9984091 A JP9984091 A JP 9984091A JP H04311322 A JPH04311322 A JP H04311322A
- Authority
- JP
- Japan
- Prior art keywords
- plant
- mycorrhizal fungi
- days
- soil
- seedlings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 20
- 241000233866 Fungi Species 0.000 claims abstract description 31
- 239000002689 soil Substances 0.000 claims abstract description 18
- 238000005520 cutting process Methods 0.000 claims abstract description 4
- 238000011081 inoculation Methods 0.000 claims abstract 3
- 238000009331 sowing Methods 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 abstract description 28
- 208000015181 infectious disease Diseases 0.000 abstract description 13
- 244000061458 Solanum melongena Species 0.000 abstract description 7
- 235000002597 Solanum melongena Nutrition 0.000 abstract description 7
- 235000007688 Lycopersicon esculentum Nutrition 0.000 abstract description 4
- 240000003768 Solanum lycopersicum Species 0.000 abstract description 4
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 abstract description 3
- 240000008384 Capsicum annuum var. annuum Species 0.000 abstract description 3
- 241000235502 Gigaspora margarita Species 0.000 abstract description 2
- 230000012010 growth Effects 0.000 abstract description 2
- 238000003973 irrigation Methods 0.000 abstract description 2
- 230000002262 irrigation Effects 0.000 abstract description 2
- 240000004160 Capsicum annuum Species 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 241000235503 Glomus Species 0.000 description 5
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 241000592780 Entrophospora infrequens Species 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- GZUXJHMPEANEGY-UHFFFAOYSA-N bromomethane Chemical compound BrC GZUXJHMPEANEGY-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 239000003864 humus Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000012192 staining solution Substances 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 241001123615 Acaulospora Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000003086 Cynanchum laeve Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241001123595 Entrophospora Species 0.000 description 1
- 241001674890 Funneliformis caledonium Species 0.000 description 1
- 241001123597 Funneliformis mosseae Species 0.000 description 1
- 241000235500 Gigaspora Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 235000010842 Sarcandra glabra Nutrition 0.000 description 1
- 240000004274 Sarcandra glabra Species 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- LFHISGNCFUNFFM-UHFFFAOYSA-N chloropicrin Chemical compound [O-][N+](=O)C(Cl)(Cl)Cl LFHISGNCFUNFFM-UHFFFAOYSA-N 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229940102396 methyl bromide Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910001562 pearlite Inorganic materials 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Abstract
Description
【0001】0001
【産業上の利用分野】本発明は植物の栽培方法に関し、
詳しくはVA菌根菌を接種して5日以上経過した用土に
植物を栽培する方法に関する。[Industrial Application Field] The present invention relates to a method for cultivating plants.
Specifically, the present invention relates to a method for cultivating plants in soil that has been inoculated with VA mycorrhizal fungi for 5 days or more.
【0002】0002
【従来の技術及び発明が解決しようとする課題】VA菌
根菌は、植物の根に共生することにより植物の生長を促
進したり、耐病性,耐乾燥性などを高める働きがあるこ
とは古くから知られている(鈴木達彦、農業および園芸
、第62巻、第8号、1987年)。VA菌根菌を植物
に感染させる方法としては、例えば播種,挿し芽もしく
は苗の移植と同時に該VA菌根菌を用土と混ぜたり、植
穴に該VA菌根菌を入れたりする方法が一般的に行われ
ている。その他、既に移植されている植物の根元に穴を
あけ、根の近傍に該VA菌根菌を入れる方法もある。[Prior Art and Problems to be Solved by the Invention] It has long been known that VA mycorrhizal fungi have the ability to promote plant growth and increase disease resistance and drought resistance by coexisting with plant roots. (Tatsuhiko Suzuki, Agriculture and Horticulture, Vol. 62, No. 8, 1987). Common methods for infecting plants with VA mycorrhizal fungi include, for example, mixing the VA mycorrhizal fungi with soil at the same time as sowing, cuttings, or transplanting seedlings, or placing the VA mycorrhizal fungi in the planting hole. It is being carried out according to Another method is to make a hole at the base of the plant that has already been transplanted and introduce the VA mycorrhizal fungus near the root.
【0003】しかし、上記した従来の一般的方法でVA
菌根菌を植物に感染させようとしても、VA菌の胞子の
発芽が不十分なため、感染率が低く、VA菌投与の効果
が十分に得られていなかった。本発明の目的は、VA菌
根菌を短時間で効率よく植物に感染させ、植物の生育初
期における生育促進と病気に対する抵抗性の付与,活着
率の向上を図ることである。However, with the conventional general method described above, VA
Even when attempting to infect plants with mycorrhizal fungi, the germination of VA fungus spores is insufficient, resulting in a low infection rate and the effect of VA fungus administration being insufficient. The purpose of the present invention is to efficiently infect plants with VA mycorrhizal fungi in a short period of time, to promote growth in the early stages of plant growth, impart resistance to diseases, and improve the survival rate.
【0004】0004
【課題を解決するための手段】すなわち本発明は、植物
を栽培するにあたり、用土にVA菌を接種して5日以上
経過した後、該用土に植物を播種,挿し芽もしくは苗移
植することを特徴とする植物の栽培方法を提供するもの
である。[Means for Solving the Problems] In other words, the present invention provides a method for cultivating plants by sowing, cuttings, or transplanting seedlings into the soil after 5 days or more have elapsed after inoculating the VA bacteria into the soil. It provides a method for cultivating characteristic plants.
【0005】VA菌は土壌中に存在する真菌類であり、
その菌糸が植物の根について菌根を形成し、両者が共生
するものである。VA菌根菌としては、例えばギガスポ
ラ(Gigaspora)属〔ギガスポラ・マルガリー
タ(G.margarita)など〕,アカウロスポラ
(Acaulospora)属〔アカウロスポラ・レー
ビス(A.laevis) など〕,エントロフォスポ
ラ(Entrophospora)属〔エントロフォス
ポラ・インフレクエンス(E.infrequens)
など〕,スクレロシスティス(Sclerocyst
is) 属〔スクレロシスティス・ドゥッシー(S.d
ussii) など〕,スカテリスポラ(Scutel
lispora)属〔スカテリスポラ・グレゴリア(S
.gregoria) など〕,グロムス(Gromu
s) 属〔グロムス・モセアエ(G.mosseae)
など〕等が知られている。[0005] VA fungi are fungi that exist in soil.
The hyphae form mycorrhizae on plant roots, and the two coexist. Examples of VA mycorrhizal fungi include the genus Gigaspora [such as G. margarita], the genus Acaulospora [such as A. laevis], and the genus Entrophospora. [ Entrophospora infrequens (E.infrequens)
etc.], Sclerocystis (Sclerocyst)
is) Genus [Sclerocystis doucii (S.d.
ussii) etc.], Scutellispora (Scutel)
lispora) genus [Scatellispora gregoria (S
.. gregoria), Gromu
s) Genus [G. mosseae]
etc.] are known.
【0006】これらVA菌根菌は、自然界から集めるほ
か、栄養薄膜培養法(特開昭55−118390号公報
),炭肥料を用いて培養する方法(特開昭60−497
17号公報),器官培養した根を使用する方法(特開昭
62−19028号公報)なとにより増殖させることが
できる。VA菌根菌の施用方法は任意であり、例えば後
述の実施例に示すように、用土とVA菌根菌を混合する
方法、種や苗の下にVA菌根菌を層状に施用する方法、
苗などの移植時に植穴にVA菌根菌を施用する方法など
がある。本発明では、VA菌根菌の施用後、5日以上経
過してVA菌根菌胞子の発芽が十分に認められてから植
物の播種等を行うことが重要である。[0006] These VA mycorrhizal fungi can be collected from the natural world, as well as by the nutrient thin film culture method (Japanese Unexamined Patent Publication No. 118390/1982), and the method of culturing using charcoal fertilizer (Japanese Unexamined Patent Publication No. 60/497).
17), a method using organ-cultured roots (Japanese Patent Application Laid-open No. 19028/1983), etc. The method of applying VA mycorrhizal fungi is arbitrary; for example, as shown in the examples below, a method of mixing VA mycorrhizal fungi with soil, a method of applying VA mycorrhizal fungi in a layer under seeds or seedlings,
There is a method of applying VA mycorrhizal fungi to the planting hole when transplanting seedlings, etc. In the present invention, it is important to sow plants after 5 days or more have passed after applying the VA mycorrhizal fungi and germination of the VA mycorrhizal fungus spores has been sufficiently observed.
【0007】VA菌根菌の植物への感染は、既知の手法
により行えばよく、例えば温度5〜60℃、好ましくは
10〜45℃、土壌pH3〜9.5、好ましくは4〜7
.5の条件で灌水を十分に行うことが望ましい。次に、
本発明の対象とされる植物については特に制限はないが
、ナス,トマト,ピーマン,シシトウなどのナス科植物
が好適である。[0007] Infection of plants with VA mycorrhizal fungi may be carried out by known methods, for example, at a temperature of 5 to 60°C, preferably 10 to 45°C, and a soil pH of 3 to 9.5, preferably 4 to 7.
.. It is desirable to perform sufficient irrigation under the conditions described in 5. next,
There are no particular limitations on the plants that are the targets of the present invention, but solanaceous plants such as eggplants, tomatoes, green peppers, and shishito peppers are suitable.
【0008】[0008]
【実施例】以下に、本発明を実施例により詳しく説明す
る。
実施例1
パーライト,バーミキュライトまたは黒ボク土をクロル
ピクリンで消毒後、ガス抜きを十分に行った。これら3
種の用土を50×35×4cmの育苗箱にそれぞれ7リ
ットル入れた。しかる後、VA菌根菌グロムス・モセア
エの胞子3200個を加え、十分に混合した。さらに灌
水を十分に行ったのち、直ちにナス(品種:黒陽)の種
を播いた区と、3日,5日,9日または12日経過後に
ナス(品種:黒陽)の種を播いた区を用意した。育苗箱
は温度20℃の人工気象器に入れ、用土が乾燥しないよ
うに灌水を行い、4000ルックス,明12時間,暗1
2時間で管理した。播種後21日目に各育苗箱より5本
の苗を取り、下記の方法でVA菌根菌の感染率を算出し
、平均値を求めた。結果を表1に示す。[Examples] The present invention will be explained in detail below using examples. Example 1 After disinfecting pearlite, vermiculite, or black earth with chloropicrin, the gas was sufficiently degassed. These 3
Seven liters of seed soil was placed in each 50 x 35 x 4 cm seedling raising box. Thereafter, 3,200 spores of the VA mycorrhizal fungus Glomus moceae were added and thoroughly mixed. Furthermore, after sufficient watering, eggplant (variety: Kuroyo) seeds were sown immediately in some plots, and eggplant (variety: Kuroyo) seeds were sown after 3, 5, 9, or 12 days. We have prepared a district. The seedling box was placed in an artificial climate chamber at a temperature of 20℃, watered to prevent the soil from drying out, and kept at 4000 lux, 12 hours of light, and 1 hour of darkness.
I managed it in 2 hours. On the 21st day after sowing, five seedlings were taken from each nursery box, and the infection rate of VA mycorrhizal fungi was calculated using the method described below, and the average value was determined. The results are shown in Table 1.
【0009】感染率の測定法(トリパンブルーによる染
色)
(ア)感染させた植物の根を水で十分に洗う。
(イ)水切りした根をビーカーに入れ、10%KOHで
1時間弱く沸騰させながら煮る。
(ウ)根が着色している場合、10%KOH ですすい
だ後、10倍に薄めたH2O2 (約3%)で5分間脱
色し、次いで水洗する。
(エ)ラクトフェノール−トリパンプルー染色液に根を
浸し5〜30分間弱く沸騰させる。なお、ラクトフェノ
ール−トリパンプルー染色液(1リットル)の組成は以
下の通りである。
フェノール 200ml
蒸留水 200ml
乳酸 200
ml グリセリン 400m
l トリパンプルー 1g
────────────────
1000ml, 0.1
%トリパンブルー(オ)根を水洗し、顕微鏡で観察する
。
(カ)1cm間隔のグリットの上に染色したサンプルを
載せ、顕鏡により確認し100個程度顕鏡し、感染数を
測定し、感染数を全計測数で除して百分率にて感染率を
求める。Method for measuring infection rate (staining with trypan blue) (a) Wash roots of infected plants thoroughly with water. (b) Place the drained roots in a beaker and boil in 10% KOH for 1 hour while gently boiling. (c) If the roots are colored, rinse with 10% KOH, decolorize with 10 times diluted H2O2 (approximately 3%) for 5 minutes, and then wash with water. (d) Immerse the roots in lactophenol-trypan blue staining solution and boil gently for 5 to 30 minutes. The composition of the lactophenol-trypan blue staining solution (1 liter) is as follows. phenol 200ml
Distilled water 200ml
lactic acid 200
ml glycerin 400m
l trypan blue 1g
──────────────────
1000ml, 0.1
%Trypan Blue (O) Wash the roots with water and observe them under a microscope. (f) Place the stained sample on grids spaced at 1 cm intervals, check using a microscope, measure about 100 samples, measure the number of infections, and calculate the infection rate as a percentage by dividing the number of infections by the total number of measurements. demand.
【0010】0010
【表1】
表 1 用 土
VA菌根菌添加後播 感染率
種までの期間(日)
(%) ─────────────
──────────── パーライト
0
1.8
3
2.6
5 15
9 31
12
26 バーミキュライト
0 0
.1
3 0.3
5 10
9 14
12
21 黒ボク土
0
0
3 0.2
5 8
9 13
12
10 ────────────
─────────────[Table 1]
Table 1 Soil
Infection rate after adding VA mycorrhizal fungi
Time to seed (days)
(%) ──────────────
──────────── Perlite
0
1.8
3
2.6
5 15
9 31
12
26 Vermiculite
0 0
.. 1
3 0.3
5 10
9 14
12
21 Kurobokuchi
0
0
3 0.2
5 8
9 13
12
10 ────────────
──────────────
【0011】実施例2
腐葉土を5mm目の篩にかけ、臭化メチルで殺菌後、十
分にガス抜きを行った。これを用土として50×35×
4cmの育苗箱に7リットル入れた。そこに、VA菌根
菌グロムス・カレドニウムおよびグロムス・モセアエの
胞子をそれぞれ3200個,8700個加え、十分に混
合した。次いで、水を十分に与えたのち直ちにトマト(
品種:ファーストメモリー)の種を播いた区、播種の3
日前,5日前,7日前または10日前に同様の処理をし
た後トマト(品種:ファーストメモリ)の種を播いた区
を用意した。なお、種は80粒播いた。播種より25日
後に各育苗箱より20本の苗を取り、実施例1と同様の
方法で感染率を測定した。20本の平均値を表2に示す
。
また、各区より20本の苗を取り、草丈を測定し、平均
値を表3に示した。Example 2 Leaf humus was passed through a 5 mm sieve, sterilized with methyl bromide, and then thoroughly degassed. Use this as soil for 50 x 35 x
I put 7 liters into a 4cm seedling box. 3,200 and 8,700 spores of the VA mycorrhizal fungi Glomus caledonium and Glomus moceae were added thereto and thoroughly mixed. Next, add plenty of water and immediately add tomatoes (
Variety: First Memory), sowing area, sowing 3
A plot was prepared in which tomato (variety: First Memory) seeds were sown after the same treatment had been performed 1 day, 5 days, 7 days, or 10 days before. In addition, 80 seeds were sown. Twenty-five days after sowing, 20 seedlings were taken from each nursery box, and the infection rate was measured in the same manner as in Example 1. Table 2 shows the average value of the 20 pieces. In addition, 20 seedlings were taken from each plot and the plant heights were measured, and the average values are shown in Table 3.
【0012】0012
【表2】[Table 2]
【0013】[0013]
【表3】[Table 3]
【0014】実施例3
実施例2において用いたVA菌根菌無添加の殺菌腐葉土
で満たした播種箱(50×35×4cm)にナス(品種
:千両二号),ピーマン(品種:鈴みどり)およびシシ
トウ(品種:翆山)を各150粒播いた。一方、上記と
同じ殺菌腐葉土1リットル当りVA菌根菌グロムス・モ
セアエの胞子を1300個加えた用土をビニールカップ
(φ7.5cm)に詰め、水を十分に与え25℃で3日
,5日,7日または10日放置したものと、1日も放置
しなかったものを用意し、これに播種後22日を経た各
種苗を20本づつ植えた。移植13日後に各種苗5本づ
づを取り、実施例1と同様の方法で感染率を測定した。
結果を表4に示す。また、残りの苗は、さらに栽培を続
け、20日後の草丈を測定し、平均値を表5に示した。
なお、対照としてVA菌根菌を全く使用しなかったこと
以外は実施例と同様に行ったものの結果も示した。Example 3 In a seeding box (50 x 35 x 4 cm) filled with the sterilized leaf mold without the addition of VA mycorrhizal fungi used in Example 2, eggplants (variety: Senryo Nigo) and green peppers (variety: Suzumidori) were placed. and Shishito (variety: Suiyama) were sown at 150 seeds each. On the other hand, fill a plastic cup (φ7.5 cm) with soil containing 1,300 spores of the VA mycorrhizal fungus Glomus moceae per liter of sterilized leaf humus as above, water thoroughly, and store at 25°C for 3 or 5 days. One that had been left for 7 or 10 days, and one that had not been left for one day were prepared, and 20 seedlings of each type were planted 22 days after sowing. Thirteen days after transplanting, five seedlings of each type were taken and the infection rate was measured in the same manner as in Example 1. The results are shown in Table 4. In addition, the remaining seedlings were further cultivated, and the plant heights were measured after 20 days, and the average values are shown in Table 5. As a control, the results were also shown in the same manner as in the example except that no VA mycorrhizal fungi were used.
【0015】[0015]
【表4】
表
4 放置日数
感染率(%) (日)
ナ ス ピーマン シシト
ウ ────────────────────
───── 0
4 1
3 3 6
5 9
5 20
17 18
7 27 20
30 10
10 11 16
─────────────────────
────[Table 4] Table 4 Number of days left unused
Infection rate (%) (days)
Eggplant Green Pepper Shishito ────────────────────
────── 0
4 1
3 3 6
5 9
5 20
17 18
7 27 20
30 10
10 11 16
──────────────────────
────
【0016】[0016]
【表5】
表
5 放置日数
草 丈(cm) (日)
ナ ス ピーマン シ
シトウ ──────────────────
─────── 0
11.9 8.3
9.8 3
12.6 9.0
10.1 5
14.9 11.2
12.5 7
16.1 12.9 1
2.7 10 1
5.2 10.7 11.
6 ────────────────────
─────[Table 5] Table 5 Number of days left unused
Plant height (cm) (day)
Eggplant Green Pepper Shishito ──────────────────
─────── 0
11.9 8.3
9.8 3
12.6 9.0
10.1 5
14.9 11.2
12.5 7
16.1 12.9 1
2.7 10 1
5.2 10.7 11.
6 ────────────────────
──────
【0017】[0017]
【発明の効果】本発明の方法によれば、植物に対するV
A菌根菌の感染率を著しく高めることができ、植物の成
長を向上させることが可能である。とりわけ、立枯れし
易いナス科植物に対する効果が大きく、実用性に富む方
法である。Effect of the invention: According to the method of the present invention, V
It is possible to significantly increase the infection rate of A mycorrhizal fungi and improve plant growth. This method is especially effective against plants of the Solanaceae family, which are prone to withering, and is highly practical.
Claims (1)
菌根菌を接種して5日以上経過した後、該用土に植物を
播種,挿し芽もしくは苗移植することを特徴とする植物
の栽培方法。[Claim 1] When cultivating plants, VA is added to the soil.
A method for cultivating plants, which comprises sowing, cuttings, or transplanting seedlings into the soil after 5 days or more have elapsed since inoculation with mycorrhizal fungi.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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JP3099840A JP3014794B2 (en) | 1991-04-05 | 1991-04-05 | How to grow plants |
Applications Claiming Priority (1)
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JP3099840A JP3014794B2 (en) | 1991-04-05 | 1991-04-05 | How to grow plants |
Publications (2)
Publication Number | Publication Date |
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JPH04311322A true JPH04311322A (en) | 1992-11-04 |
JP3014794B2 JP3014794B2 (en) | 2000-02-28 |
Family
ID=14258000
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JP3099840A Expired - Fee Related JP3014794B2 (en) | 1991-04-05 | 1991-04-05 | How to grow plants |
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JP (1) | JP3014794B2 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100838687B1 (en) * | 2007-05-11 | 2008-06-16 | 충북대학교 산학협력단 | Arbuscular mycorrhizal fungi for capsicum annuum |
KR100852771B1 (en) * | 2008-05-13 | 2008-08-18 | 충북대학교 산학협력단 | Arbuscular mycorrhizal fungi for capsicum annuum |
CN104938227A (en) * | 2015-06-27 | 2015-09-30 | 蚌埠市双墩农业生物科技开发有限责任公司 | Method for increasing survival rate of seedlings after tomatoes are transplanted |
CN107493861A (en) * | 2017-08-09 | 2017-12-22 | 李晓枫 | A kind of capsicum method for non-polluted cultivation |
CN107787773A (en) * | 2017-10-30 | 2018-03-13 | 务川自治县九叶青花椒有限责任公司 | A kind of breeding method of Chinese prickly ash seedling |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH04128742U (en) * | 1991-05-14 | 1992-11-25 | 徳治郎 平沼 | Pressure mats on the soles of the feet |
-
1991
- 1991-04-05 JP JP3099840A patent/JP3014794B2/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH04128742U (en) * | 1991-05-14 | 1992-11-25 | 徳治郎 平沼 | Pressure mats on the soles of the feet |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100838687B1 (en) * | 2007-05-11 | 2008-06-16 | 충북대학교 산학협력단 | Arbuscular mycorrhizal fungi for capsicum annuum |
KR100852771B1 (en) * | 2008-05-13 | 2008-08-18 | 충북대학교 산학협력단 | Arbuscular mycorrhizal fungi for capsicum annuum |
CN104938227A (en) * | 2015-06-27 | 2015-09-30 | 蚌埠市双墩农业生物科技开发有限责任公司 | Method for increasing survival rate of seedlings after tomatoes are transplanted |
CN107493861A (en) * | 2017-08-09 | 2017-12-22 | 李晓枫 | A kind of capsicum method for non-polluted cultivation |
CN107787773A (en) * | 2017-10-30 | 2018-03-13 | 务川自治县九叶青花椒有限责任公司 | A kind of breeding method of Chinese prickly ash seedling |
Also Published As
Publication number | Publication date |
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JP3014794B2 (en) | 2000-02-28 |
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