JPH04211308A - Artificial culture of lyophyllum decastes - Google Patents

Abstract

PURPOSE:To provide an artificial raising method capable of producing Lyophyllum decastes in high grade at low cost in a short period as well as in high yield on an industrial scale in facilities. CONSTITUTION:Lyophyllum decastes strain is raised using a conventional artificial raising technique with mushroom bed. The raising method is e.g. one through bottle culture, bag culture, box culture. The present invention can also provide three kinds of novel Lyophyllum decastes strain, raising tasty Lyophyllum decastes of favorable form in high yield in a short period.

Description

[Detailed description of the invention]

0001

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for artificially cultivating Hatakeshimeji (scientific name: Lyophyllum decastes), which is useful as an edible mushroom.

0002

[Prior Art] Hatakeshimeji is a mushroom that widely grows near human residences, fields, forests, etc. from summer to autumn, and its shape is very similar to Honshimeji. The taste is very good, and the flesh is firmer and crisper than hon-shimeji mushrooms, making it a favorite edible mushroom. In recent years, an artificial fungal bed cultivation method has been established for cultivating enokitake, oyster mushroom, bunashimeji mushroom, nameko mushroom, etc. using a culture medium that is mainly a mixture of sawdust and rice bran.
Mushrooms can now be harvested consistently throughout the year, regardless of the seasons. Since Hatakeshimeji is also useful as an edible mushroom, various cultivation methods are being studied.

0003

[Problems to be Solved by the Invention] However, Hatakeshimeji is said to be difficult to cultivate using ordinary logs because of its saprophytic fungi. At the Fukushima Prefectural Forestry Experiment Station, we are considering a bag cultivation method that uses bark compost as the main medium and rice bran and bran as nutritional additives, as well as a natural cultivation method that involves burying the medium outdoors. According to a cultivation test using bag cultivation using 1 kg of a medium with a weight ratio of bark compost and rice bran of 10:1.5 and a moisture content of 65% at the time of preparation, the development period of Hatake Shimeji fruiting bodies was over a long period of time.
Looking at the total number of days since inoculation during which there was no intensive occurrence and the occurrence rate was the highest, it took a long time to cultivate, at 180 to 240 days, although there were differences among the tested bacterial strains. ing. In addition, many harmful bacteria occur during cultivation, and it has been reported that this cultivation method is inefficient (Fukushima Prefecture Forestry Experiment Station Research Report No. 19). Therefore, we have started considering a natural cultivation method that involves burying the culture medium outdoors (Fukushima Prefecture Forestry Experiment Station Research Report No. 20). Furthermore, in Japanese Patent Application Laid-Open No. 63-169913, sawdust 100
In contrast, chicken manure, humus, ash, and bran were added at 0.5 to 0.0% each.
A method for cultivating Hatakeshimeji mushrooms in bottles using a medium mixed at a weight ratio of 6:6 is described, but this cultivation method differs from the usual method of cultivating mushrooms in bottles by reversing the opening of the bottle after scraping the bacteria and pouring water. The process involves cultivating mushrooms for about a week, then returning them to their original state with the bottle mouth facing up, and cultivating them again, which is more complicated and less workable than the normal mushroom bottle cultivation method.

In view of the above-mentioned current situation, the object of the present invention is to provide a method for artificially cultivating Hatakeshimeji, which is a useful edible mushroom, by which it can be produced industrially in a facility, with high quality, at low cost, and efficiently in a short period of time. Our goal is to provide the following.

[0005]

[Means for Solving the Problems] To summarize the present invention, the present invention relates to a method for artificially cultivating Hatakeshimeji mushrooms, and is characterized in that Hatakeshimeji fungus strains are cultivated using a conventional artificial bed cultivation method.

[0006] Generally speaking, it is known that even though mushrooms belong to the same species, the growth rate of hyphae and the ability to form fruit bodies vary significantly depending on where they are collected. The present inventors have discovered that strains suitable for ordinary artificial cultivation in bacterial beds are
Based on the belief that Hatakeshimeji must exist in nature, we collected Hatakeshimeji mushrooms from various places and conducted extensive research.We found that the strains collected in Switzerland form fruiting bodies easily and in high yields. The present invention has been completed.

[0007] Examination of bacterial strains was conducted as follows. P.G.
Y liquid medium (composition: glucose 2.0%, peptone 0.
2%, yeast extract 0.2%, KH2 PO4 0.0
5% and 0.05% of MgSO4.7H2O, pH
6.0) Inoculate each strain of Hatakeshimeji into 100ml,
It was cultured at 25° C. for 10 days and used as a liquid inoculum. 50g of humus in a polypropylene wide-mouth culture bottle (850ml),
Add 350g of water to 50g of sawdust and 100g of rice bran, mix well, pressurize the wet mixture, and place it in the center with a diameter of 1cm.
A hole of approximately m diameter was made, the tube was capped, and the tube was sterilized at 120° C. for 60 minutes to prepare a solid culture medium. Add 20m of each of the above liquid starter bacteria to this.
The cells were inoculated and cultured in the dark at a temperature of 25° C. and a humidity of 55% until the culture medium was covered with apparently mycelia, and the culture was continued for an additional 30 days to ripen. Next, remove the mycelial layer of about 1 cm from the top of the culture medium by scraping the bacteria, then add tap water up to the bottle opening, let it stand for 3 hours, then drain it.
Cultivation was continued under conditions of a temperature of 15° C. and a humidity of 90% until fruiting body primordia were formed. The culture medium in which the primordium has been formed is then exposed to illuminance of 500 lux, temperature of 15°C, and humidity of 9.
Continue culturing under 0% conditions until mature fruiting bodies are obtained.
The fruiting body yield, total number of cultivation days, and fruiting body shape of each strain of Hatakeshimeji were investigated. The results are shown in Table 1.

[0008]

[Table 1]

[0009] In Table 1, "unsatisfactory" refers to a case in which fruiting bodies are not formed even after a total of 180 cultivation days. Also,
The shapes in Table 1 are as follows: ◎ means that the shape of the fruiting body is excellent;
○ indicates that the shape of the fruiting body is good, and × indicates that the shape of the fruiting body is poor.

[0010] As is clear from Table 1, among the tested strains, three strains, K-3303, K-3304, and K-3305, have a short cultivation period of about 90 days, and have a yield of about 14
It weighed more than 0g, and the cultivated fruit bodies also showed particularly excellent properties, with the cap color, stalk color, shape, etc., being the same as those of naturally collected fruit. For each strain shown in Table 1, the above-mentioned JP-A-63-
A cultivation test was conducted in accordance with the method disclosed in Publication No. 169913, adding the steps of scraping the bacteria and inverting the mouth of the bottle after pouring water, but no significant effect was observed, and rather a decrease in yield.

Among the Hatakeshimeji strains shown in Table 1, K.
- The morphological characteristics of the fruiting bodies and spores of the strains indicated by numbers are:
It is as follows.

[0012] The fruiting body grows in clusters, and the cap is approximately 5 cm in diameter, broadly convex, and almost circular. The surface is grayish brown, and the color becomes lighter in older ones. The center of the cap is especially covered with slightly powdery velvet hairs, and the edges are curled downward. The flesh is white in color and has a slightly powdery aroma. The folds are white with a slight yellow tinge and are dense. The handle is 5cm long
It is approximately 1 cm thick, the top and bottom are the same size, or slightly swollen at the bottom, light gray in color, hard and elastic, and tightly packed. Spores are smooth and spherical or nearly spherical. 5.5-7.5
×5 to 7 μm.

[0013] The above characteristics are summarized in “Illustrated Encyclopedia of New Japanese Fungi in Primary Colors (I)” edited by Rokuya Imaseki and Tsuguo Hongo, published by Yokusha (June 1, 1986).
When compared with the description in ``Hatakeshimeji'' (first edition published on June 0), it is clear that these strains are Hatakeshimeji.

[0014] Among these test bacterial strains, K-3303 strain
Lyophyllum decastes K-330
The K-3304 strain was designated as Lyophyllum de
castes K-3304, and as FERM P-11321,
K-3305 strain is Lyophyllum decas
Displayed as tes K-3305, Microtechnical Research Institute No. 113
No. 22 (FERM P-11322).

[0015] Next, the bacterial strain deposited at the Institute of Fine Technology, Hatakeshimeji K-
The mycological properties of the three strains, 3303 strain, K-3304 strain, and K-3305 strain, are shown below.

(1) Hatakeshimeji strain K-3303 ■ Growth status on malt extract agar medium (20° C.) On the 10th day, the colony diameter was 28 mm, producing white, dense hyphae and aerial hyphae. On the 15th day, the colony diameter was 48 mm, 20
On the first day, the colony diameter was 67 mm, and the hyphae were white and dense.
Stretch in a straight line. There are many aerial mycelia. The back side is uniform with no discoloration. ■ Growth status on potato-glucose agar medium (20°C) On the 10th day, the colony diameter was 28 mm, producing a large amount of white, dense hyphae and aerial hyphae. Colony diameter was 49mm on the 15th day.
On the 20th day, the colony diameter was 64 mm, and the hyphae were white, dense, and mat-like. There are a lot of aerial mycelia. The back side is uniform with no discoloration. ■ Growth status on oatmeal agar medium (20°C) On the 10th day, the colony diameter was 34 mm, and the hyphae were thin and extended radially. On the 15th day, the colony diameter was 58 mm, and on the 20th day, the colony diameter was 80 mm, and the hyphae were white and extended radially. Aerial mycelium is thin but becomes thicker on the 20th day. The back side is uniform with no discoloration. ■ Growth status in phenol oxidase assay medium [potato glucose agar medium supplemented with 0.1% gallic acid] (20°C) Growth was poor on the 10th day, colony diameter was 9 mm, hyphae were white, and there were many aerial hyphae. Browning radius is 35mm. On the 20th day, the colony diameter was 15 mm and the browning radius was 50 mm. The mycelium is white and swollen. ■ Optimum growth temperature PGY agar medium (PGY liquid medium with agar added)
Inoculum with a diameter of 6 mm was inoculated into the cells, cultured at various temperatures, and the diameter of each Corot was measured after 14 days, and the optimum growth temperature was found to be around 25°C. Moreover, it hardly grew at 5°C and did not grow at all at 30°C. (2) Optimal growth pH After sterilizing 40 ml of PGY liquid medium, it was adjusted to various pH values, inoculated with a seed culture of 6 mm in diameter, and cultured at 25° C. for 14 days. After collecting the bacteria, it was dried and weighed, and the optimum pH was found to be around 6. In addition, the growth range of this strain is from pH 4 to
The pH was between 9 and 9.

(2) Hatakeshimeji K-3304 strain ■ Growth status on malt extract agar medium (20° C.) On the 10th day, the colony diameter was 25 mm, producing white, dense hyphae and aerial hyphae. On the 15th day, the colony diameter was 44 mm, 20
On the first day, the colony diameter was 65 mm, and the hyphae were white and dense.
Stretch in a straight line. There are many aerial mycelia. The back side is uniform with no discoloration. ■ Growth status on potato-glucose agar medium (20°C) On the 10th day, the colony diameter was 32 mm, producing a large amount of white, dense hyphae and aerial hyphae. On the 15th day, the colony diameter was 53 mm.
On the 20th day, the colony diameter was 69 mm, and the hyphae were white, dense, and mat-like. There are a lot of aerial mycelia. The back side is uniform with no discoloration. ■ Growth status on oatmeal agar medium (20°C) On the 10th day, the colony diameter was 31 mm, and the hyphae were thin and extended radially. On the 15th day, the colony diameter was 55 mm, and on the 20th day, the colony diameter was 76 mm, and the hyphae were white and extended radially. Aerial mycelium is thin but becomes thicker on the 20th day. The back side is uniform with no discoloration. ■ Growth status in phenol oxidase assay medium (20°C) On the 10th day, growth was poor, colony diameter was 9 mm, hyphae were white, and there were many aerial hyphae. Browning radius is 36mm. On the 20th day, the colony diameter was 18 mm and the browning radius was 52 mm. The mycelium is white and swollen. (2) Optimal Growth Temperature A 6 mm diameter inoculum was inoculated onto a PGY agar medium, cultured at each temperature, and the Corot diameter was measured 14 days later, and the optimum growth temperature was found to be around 25°C. Also, 5℃
It hardly grew at 30°C, and it did not grow at all at 30°C. (2) Optimal growth pH After sterilizing 40 ml of PGY liquid medium, it was adjusted to various pH values, inoculated with a seed culture of 6 mm in diameter, and cultured at 25° C. for 14 days. After collecting the bacteria, it was dried and weighed, and the optimum pH was found to be around 5. In addition, the growth range of this strain is from pH 4 to
The pH was between 9 and 9.

(3) Hatakeshimeji strain K-3305 ■ Growth status on malt extract agar medium (20° C.) On the 10th day, the colony diameter was 20 mm, producing white, dense hyphae and aerial hyphae. On the 15th day, the colony diameter was 35 mm, 20
On the first day, the colony diameter was 49 mm, and the hyphae were white and dense.
Stretch in a straight line. There are many aerial mycelia. The back side has radial wrinkles in the center, but no discoloration. ■ Growth status on potato-glucose agar medium (20°C) On the 10th day, the colony diameter was 27 mm, producing a large amount of white, dense hyphae and aerial hyphae. Colony diameter was 42mm on the 15th day.
On the 20th day, the colony diameter was 57 mm, and the hyphae were white, dense, and mat-like. There are a lot of aerial mycelia. The back side has radial wrinkles in the center, but no discoloration. ■ Growth status on oatmeal agar medium (20°C) On the 10th day, the colony diameter was 33 mm, and the hyphae were thin and extended radially. On the 15th day, the colony diameter was 53 mm, and on the 20th day, the colony diameter was 73 mm, and the hyphae were white and extended radially. Aerial mycelium is thin even on the 20th day. The back side has radial wrinkles in the center, but no discoloration. ■ Growth status in phenol oxidase assay medium (20°C) On the 10th day, growth was poor, colony diameter was 11 mm, hyphae were white, and there were many aerial hyphae. The browning radius is 30mm. On the 20th day, the colony diameter was 14 mm and the radius of browning was 43 mm. The mycelium is white and swollen. (2) Optimal Growth Temperature Inoculum with a diameter of 6 mm was inoculated onto a PGY agar medium, cultured at each temperature, and the diameter of each colony was measured after 14 days. The optimum growth temperature was found to be around 25°C. Also, 5
There was almost no growth at 30°C, and no growth at all at 30°C. (2) Optimal growth pH After sterilizing 40 ml of PGY liquid medium, it was adjusted to various pH values, inoculated with a seed culture of 6 mm in diameter, and cultured at 25° C. for 14 days. After collecting the bacteria, it was dried and weighed, and the optimum pH was found to be around 5. In addition, the growth range of this strain is from pH 4 to
The pH was between 9 and 9.

Furthermore, Hatakeshimeji K-3303 strain, K-
The differences between the 3304 strain and the K-3305 strain and other Hatakeshimeji were investigated by counterculture on an agar medium. All 15 Hatakeshimeji strains shown in Table 1 were tested. The dikaryotic hyphae of the test bacterial strain were preserved on a slant (PG
A 3 mm x 3 mm x 3 mm block was cut out from a PGY agar slant medium, and each block was inoculated in the center of a PGY agar plate (2 cm apart). After culturing at 25°C for 20 days, a band was formed at the border of both colonies. It was determined whether a line appeared. The results are shown in Table 2.

[0020]

[Table 2]

As shown in Table 2, K-3303 strain, K
It is clear that the three strains, -3304 strain and K-3305 strain, are new strains because they formed bands with all the tested bacterial strains.

[0022] The Hatakeshimeji fungus strain of the present invention can be cultivated by a conventional artificial bed cultivation method.

To explain in more detail, the ordinary artificial bed cultivation method is a method used for cultivating mushrooms such as enokitake, oyster mushroom, and bunashimeji, and includes bottle cultivation,
These include bag cultivation, box cultivation, etc., but here we will talk about bottle cultivation as an example.The method usually involves the following steps: culture medium preparation, bottling, sterilization, inoculation, culturing, bacterial scraping, sprouting, growth, and harvesting. Consisting of Culture medium preparation is the process of adding water to a mixture of sawdust, rice bran, bran, crushed barley, etc., which is usually used for artificial cultivation of mushrooms, to make it moist. It is preferable to add compost, etc., and the water content is suitably 60 to 75%, preferably around 65%. The composition of the medium may be any composition that favors the formation of fruit bodies of Hatakeshimeji, and one example is a combination of sawdust, leaf humus, and rice bran. Sawdust acts as a medium base material, rice bran acts as a nutrient source, and leaf mold acts as a growth factor for saprophytic bacteria, for example. The amount of humus to be added to the culture medium may be 1% or more by weight, preferably 5% or more. Bottling means 800~
Place 450 g of the prepared medium in a 1000 ml, preferably 850 ml, polypropylene wide-mouth culture bottle.
It is a process of compressing 50g, preferably 550g, making a hole of about 1cm in the center, and plugging. Sterilization is a process in which all microorganisms in the culture medium are killed using steam. For normal pressure sterilization, the temperature is 98℃ for 4 to 5 hours, and for high pressure sterilization, the temperature is 120℃ for 30 hours.
Runs for ~90 minutes. Inoculation is the process of planting seed bacteria into a medium that has been left to cool. As the seed bacteria, Hatakeshimeji fungus strain cultured in a PGY liquid medium at 25°C for 10 to 15 days can be used, and each bottle contains about 20 ml of sterile bacteria. to plant. In addition, the culture medium inoculated with the liquid inoculum obtained through the steps described up to this point is cultured at 25°C for 30 to 40 days, and the mycelia of Hatakeshimeji are spread throughout the culture medium, which can be used as a solid inoculum. 15
Plant in a sterile manner. Cultivation is a process of spreading mycelium on the inoculated culture medium at a temperature of 20 to 25°C and a humidity of 40 to 70%, and further ripening.
It is preferably carried out for about 80 days. Scraping is the process of scraping off the inoculum and the surface of the culture medium to encourage the formation of primordia.After scraping, immediately fill the jar with water to the mouth and drain it after 3 to 5 hours. Sprouting is the process of forming fruiting body primordia, at a temperature of 10 to 20°C, preferably around 15°C, and a humidity of 80%.
If the culture is continued for 10 to 20 days at preferably 85 to 95% of the above and an illuminance of 500 lux or less, preferably 50 lux or less, Hatakeshimeji primordia are formed. What is growth?
Temperature 10 in the process of forming mature fruiting bodies from fruiting body primordia
~20°C, preferably around 15°C, humidity 80% or more, preferably 85-95%, illuminance 50 lux or more, preferably
If cultivation is continued for 5 to 15 days at 200 to 500 lux, mature fruiting bodies of Hatakeshimeji can be obtained, and the entire cultivation process is completed by harvesting. Although the bottle cultivation method has been described above, the present invention is not limited to bottle cultivation.

According to the present invention, in facility cultivation, it is possible to intensively produce well-shaped Hatakeshimeji with a yield of 100 g or more in an 850 ml culture bottle in a total cultivation period of 150 days or less.

Hatakeshimeji strains that can be used in the present invention include Hatakeshimeji K-3303 strain and Hatakeshimeji K-3 strain.
304 strain, Hatakeshimeji K-3305 strain, etc., but the present invention is not limited to these strains, and any strain having the above-mentioned properties may be used, including strains isolated from nature, hybridization, and mutation treatments. Any strain created by genetic manipulation or the like can be used.

[0026]

[Example] The method for artificially cultivating a fungus bed of Hatakeshimeji according to the present invention will be described below with examples, but the present invention is not limited to the scope of the following examples.

Example 1 PGY liquid medium (composition: 2.0% glucose, 0.2% peptone, 0.2% yeast extract, 0% KH2PO4
．． 0.05% and 0.05% of MgSO4 7H2O,
Hatakeshimeji K-3303 strain (FERM P-11320) was inoculated into 100 ml of pH 6.0) and incubated at 25°C.
It was cultured for 10 days and used as a liquid inoculum. Meanwhile, put 50 g of humus into a polypropylene wide-mouth culture bottle (850 ml).
(manufactured by Kotohira), sawdust (cedar wood) 50g, rice bran 10g
0g and 350g of water were added and mixed well to make it moist, then the mixture was compressed, a hole with a diameter of about 1cm was made in the center, and after the stopper was sealed, it was sterilized with high pressure steam at 120℃ for 60 minutes, and left to cool to form a solid culture medium. And so. This was inoculated with about 20 ml of the above liquid seed culture, and first cultured in a dark place under conditions of a temperature of 25°C and a humidity of 55% for 35 days until the hyphae appeared to cover the culture medium.
The culture was continued for another 30 days to ripen. Next, scrape the bacteria to remove about 1 cm of mycelial layer from the top of the culture medium, add tap water to the top of the bottle, let it stand for 3 hours, then drain it. 10 under conditions
Cultivation was continued for several days to form fruiting body primordium. The culture medium in which the primordium has been formed is then exposed to an illuminance of 500 lux and a temperature of 15°C.
The culture was continued for 12 days at a humidity of 90% to obtain a mature fruit body. The harvested Hatakeshimeji mushrooms were close to natural, had an excellent shape, and were extremely delicious. The weight of the resulting fruiting bodies per bottle was 147 g, and the total number of cultivation days was 87 days.

Example 2 Hatakeshimeji K-3304 in 100ml of PGY liquid medium
strain (FERM P-11321) and inoculated with 25
It was cultured at ℃ for 10 days and used as a liquid seed culture. Meanwhile, put bark compost into a polypropylene wide-mouth culture bottle (850ml).
Fujimi Kogyo Co., Ltd.] 50g, sawdust (cedar wood) 50g,
Add 100g of rice bran and 350g of water, mix well to make it moist, then pressurize the mixture, make a hole with a diameter of about 1cm in the center, and after sealing, sterilize with high-pressure steam at 120℃ for 60 minutes, and leave to cool to solidify. It was used as a culture medium. Add about 20m of the above liquid seed culture to this.
1 was inoculated and first cultured in the dark at a temperature of 25° C. and a humidity of 55% for 35 days until the culture medium was covered with mycelia, and then cultured for an additional 30 days to ripen. Next, scrape the bacteria to remove about 1 cm of mycelial layer from the top of the culture medium, add tap water to the top of the bottle, let it stand for 3 hours, then drain it. Cultivation was continued under these conditions for 11 days to form fruiting body primordia. The culture medium in which the primordium was formed was then continued to be cultured for 12 days under conditions of illuminance of 500 lux, temperature of 15° C., and humidity of 90% to obtain mature fruiting bodies. The harvested Hatakeshimeji mushrooms were close to natural, had an excellent shape, and were extremely delicious. 1 of the fruiting bodies obtained
The weight per bottle was 135 g, and the total number of cultivation days was 88 days.

Example 3 Hatakeshimeji K-3305 in 100ml of PGY liquid medium
strain (FERM P-11322) and inoculated with 25
It was cultured at ℃ for 10 days and used as a liquid seed culture. On the other hand, in a polypropylene wide-mouth culture bottle (850 ml), 50 g of leaf mold (manufactured by Kotohira), 50 g of sawdust (beech wood), and 1 ounce of rice bran.
00g and 350g of water were added and mixed well to make it moist, then the mixture was compressed, a hole with a diameter of about 1cm was made in the center, and after the stopper was sealed, it was sterilized with high-pressure steam at 120℃ for 60 minutes, and left to cool to form a solid culture medium. And so. This was inoculated with about 20 ml of the above liquid seed culture, and first cultured in a dark place under conditions of a temperature of 25°C and a humidity of 55% for 35 days until the hyphae appeared to cover the culture medium.
The culture was continued for another 30 days to ripen. Next, scrape the bacteria to remove about 1 cm of mycelial layer from the top of the culture medium, add tap water to the top of the bottle, let it stand for 3 hours, then drain it. 12 under conditions
Cultivation was continued for several days to form fruiting body primordium. The culture medium in which the primordium has been formed is then exposed to an illuminance of 500 lux and a temperature of 15°C.
The culture was continued for 13 days at a humidity of 90% to obtain a mature fruit body. The harvested Hatakeshimeji mushrooms were close to natural, had an excellent shape, and were extremely delicious. The weight of the resulting fruiting bodies per bottle was 150 g, and the total number of cultivation days was 90 days.

Example 4 350 g of water was added to 50 g of humus, 50 g of sawdust (cedar wood), and 100 g of rice bran, mixed well to make a moist state, and the mixture was compressed into a polypropylene wide-mouth culture bottle (850 ml) and placed in the center. Make a hole with a diameter of about 1 cm, and after capping, store at 120℃.
High-pressure steam sterilization was performed for 60 minutes, and the mixture was allowed to cool to obtain a solid culture medium. In addition to this, K prepared in the same manner as in Example 1 was added to the solid culture medium.
-3303 strain (FERM P-11320) was inoculated and cultured for 30 days at a temperature of 25° C. and a humidity of 55%, and a solid culture medium for the seed was obtained. Meanwhile, 50g of bark compost, 50g of sawdust (cedar wood), 100g of rice bran
A solid culture medium was prepared in the same manner as in Example 1 using 350 g of water. The inoculum from the solid culture medium for inoculum was aseptically inoculated into this, and artificial cultivation of Hatakeshimeji mushrooms was carried out as in Example 1. After a total cultivation period of 87 days, each bottle yielded 145 g of excellent shape and was very delicious. A fruiting body was obtained.

[0030]

[Effects of the Invention] As explained above in detail, according to the cultivation method of the present invention, Hatakeshimeji mushrooms, which are excellent in shape and delicious, can be cultivated in a high yield and in a short period of time.

Claims (1)

[Claims] 1. A method for artificially cultivating a Hatakeshimeji strain, which comprises cultivating the Hatakeshimeji strain using a conventional artificial bed cultivation method.