JPH04210603A - Agent for keeping activity of plant under low illumination condition - Google Patents

Abstract

PURPOSE:To provide the subject agent containing a compound having triazole group such as paclobutrazol and uniconazole as an active component, effective for controlling the useless unproductive growth of plant under low illumination condition with insufficient sunlight and prolonging the life of the plant. CONSTITUTION:The objective plant activity keeping agent contains a triazole group-containing compound as an active component and exhibits life-prolonging action to a plant cultured in an indoor illumination environment. Examples of the compound are (2RS-3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1- yl)pentane-3-ol and (E)-(RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1- yl)-1-pentene-3-ol. Preferable concentration of the compound is 1.25-20ppm for former compound and 0.2-1.0ppm for latter compound.

Description

[Detailed description of the invention]

[00011

[Industrial Application Field] This invention is suitable for use in places where sunlight does not penetrate sufficiently, such as office buildings, hotels, conference halls, Zoshita Street, or ordinary residences, where it is difficult for plants to carry out their natural photosynthesis activities. This invention relates to an agent for maintaining plant activity under illumination conditions. [0002] [0002] [0002] Plants planted under such low light conditions should be replaced with fresh plants once or twice a month to prevent their activity from declining. , or have relied on supplementary lighting for plants through artificial lighting at great energy costs. However, all of these methods have the problem of significantly increasing management costs. [0003]

[Problems to be Solved by the Invention] Due to the above-mentioned circumstances, there is currently a strong need for a plant activity maintaining agent that is safe and easy to use, and has an excellent activity maintaining effect on plants under low light conditions. has been done. An object of the present invention is to provide a plant activity maintaining agent that can meet such demands. [0004]

[Means for solving the problem] In recent years, the number of indoor living spaces has increased rapidly, especially in urban areas, and with this, there has been a remarkable tendency to install flower pots, partition planters, or indoor atriums using various green plants. . [0005] The plant activity maintaining agent according to the present invention is intended for plants planted under such indoor low-light conditions. [0006] In general, outside sunlight (for example, on a clear day, 5
□ Factors to consider when bringing plants that have been grown under conditions (from 10,000 to 100,000 lutu Among them, the most important environmental factor is light conditions. This can be easily inferred from the fact that photosynthesis plays a major role in the metabolic process of plants. [0007] Various efforts have been made to maintain the activity of plants under low light conditions for as long as possible, but the key is how to maintain a positive carbon balance in plants. It comes down to that. This means that
It includes two contents: maximally increasing the photosynthetic rate per leaf area of the plant and minimizing the respiration rate of the plant. As a result of extensive research based on this idea, we have completed the present invention. [0008] That is, the plant activity maintaining agent according to the present invention is characterized by containing a compound having a triazole group as an active ingredient. [0009] In the present invention, a representative example of a compound having a triazole group is paclobutrazol, that is, [(2R3-3RS)-1-(4-chlorophenyl)-
4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pentan-3-ol 1, uniconazole i.e. [(E)-(RS)-1-(4-chlorophenyl)-4 ,4-dimethyl-2-(1H-1,2,4
-triazol-1-yl)-1-penten-3-ol 1 and the like. However, compounds having a triazole group are not limited to these. [00101 By applying these compounds to plants by soil irrigation or foliar spraying, simultaneously with or separately from ordinary inorganic fertilizers, a remarkable effect of maintaining plant activity under low light conditions is observed.

[0011] The preferred concentration of the compound having a triazole group is, for example, 1.2 for paclobutrazol.
5 ppm to 20 ppm, and 0.2 to 1.0 ppm for uniconazole. [0012] Regarding plant cultivation methods, as shown in the Examples below, the plant activity maintaining agent of the present invention can be used in hydroponics, soil cultivation,
Both are effective for cut branches, etc. [00131 The target plant is not limited to a specific plant. Although it has a remarkable activity-maintaining effect on gramineous plants under low illuminance conditions, target plants are not limited thereto, and may also be woody plants such as goldfrost and varietal rose. [0014] The compound of the present invention has already been registered as an agricultural chemical as a plant growth regulator that has the effect of inhibiting segmental elongation (phosphorization) or increasing the number of buds set, and the safety for animals and plants, handling methods, etc. This is a substance that has been studied in the past. [0015] The compound of the present invention exhibits its effect as an active ingredient of a plant activity maintaining agent under low illuminance plant cultivation conditions such as an indoor light environment. [0016] Since the compound of the present invention is generally poorly soluble in water, it can be used by suspending and dissolving it in water using an appropriate surfactant, etc., and can also be used as a powder, wettable powder, emulsion, etc. according to the preparation method of general agricultural chemicals. It can also be used in formulations such as granules. [0017] [Effects of the Invention] The agent for maintaining plant activity under low illumination conditions according to the present invention contains a compound having a triazole group as an active ingredient, so it can be used to maintain plant activity under low light conditions. In addition to suppressing elongation, it exerts an activity-maintaining effect, that is, a life-prolonging effect. This life-prolonging effect can extend the replacement period of plants by 1.5 to 2 times. [0018]

EXAMPLES Next, some examples will be given to demonstrate the above-mentioned effects of the present invention. [0019] Example 1 Murashigeskoog modified medium (expressed in mg/l, NH
4NO3: 825, KNO3: 950, KH2PO4
:85, CaCl2-2H20:220, MgSO4・
7H20:185, KI:0.83, FeSO4・7H
20:27.8, Mn SO4・4H20: 22.
3.CuSO4・5H20: 0. O25, ZnS
O4・7H20: 8.6, COC12・6H20: 0
．． 025, Na2MoO,+ ・2H20: 0.
25, N3 BO3: 6゜2, EDTA ・2Na
・2H20: Uniconazole was added to the aqueous solution of 37.3) at concentrations of 0.25 ppm, 1°0 ppm, and 5 ppm, respectively.
．． It was dissolved to a concentration of 0 ppm, and four test plots with different concentrations were set up, including a no-addition control (pH 5, 8). As a Uniconazole standard, the product name "Sumi Seven" manufactured by Sumika Agro Co., Ltd. (contains 0.05% Uniconazole)
It was used. [00201] On the other hand, seeds of barley (variety: feed barley Kuki No. 11) were soaked in tap water at a temperature of 20°C overnight to germinate, and then germinated in a vermiculite-water system at a temperature of 20°C according to a conventional method.
8 days at °C, illuminance s, ooo lux (12 hour photoperiod)
The seedlings were cultivated under these conditions to obtain sprouts that grew to about 12 cm. These sprouts were used as test plants for testing. [00211 That is, pour 30 ml of the above test solution into a 50 ml plastic cup (1) shown in the attached drawing,
A lid (4) having a hole (3) with a diameter of 1 cm in the center was placed over the cup (1). Insert the lower part of one of the barley sprouts (5) into the cup (1) through this person (3), opening the opening so that the roots are immersed in the nutrient solution and the upper part from the seeds is above the liquid level. Wrap the barley stalks with cotton (6) at the point marked and put the lid on (
4) was fixed. The sprouts (5) were pretreated for 12 hours at a temperature of 20°C and an illuminance of 5000 lux, and then a temperature of 25°C and an illumination of 3
It was cultivated under conditions of 0.00 lux (12 hours of photoperiod). This cultivated product was subjected to an analytical test at regular intervals. Ten test individuals were used in each section. [0022] As for the analytical test items, lodging, withering, number of erect individuals, number of individuals with second leaf development, fresh weight, and photosynthetic activity per fresh weight were measured. [0023] Photosynthetic activity was measured by an oxygen electrode method (manufactured by Electrode Rank Brothers). i.e. 10
50mM HEPES with mM NaHCO3
(N2-Hydroxyethylpiperazine-N'-2-nitanesulfonic acid) -NaOH, pH 7,2
50.0 mg of chopped barley leaves were added to 4.0 ml of the reaction solution, and pre-irradiated with a temperature of 25°C and an illumination intensity of 2000 lux for 5 minutes, and then the oxygen generation rate was measured under the same temperature and illumination conditions. [0024] Test The results of the test are shown in Table 1. [0025]

[Table 1] Effect of uniconazole on barley growth under low light conditions ” Number of bodies withered and dead ● S 6!J No additive control erect individuals lost ● O ● O Rojl211m1Ijll body lost ● / / l Average fresh weight aba Note 1》 0.1Ii4 / / / 0,llfi!゜゜/ ゜゜/ Photosynthetic activity ω time 3● !Tenth 13.1 ..One piece right S ● Withering drama body failure O O O O Upright steel body failure 1● 1●: 12180N total loss / ● / Average new alre V《Note υ OJTO / / /O. !II2 (Moaning 10? / Sewing r》 ゜゜ / ゜゜ / Photosynthetic activity Q main υ Seki 2 Tomi! Snow.! Lodging mercenary defeat 1e G ■ Withered individual defeat G ● - O O ● 1.0 erect II # late 50'' - 1● Te mouth jl21g integral peg O / ● / ..I!Eyotic senma ω (Note 1) OJI1 / / / 11.111 (#IDIO? / !7Ihr) ゜゜ / ゛ / Photosynthetic activity (water) gt 1.7 Lodging individual withered and withered body drinking O l S S log- 5.0 Upright one-piece drumming a O O / ● / Four buttons υ Average new dance llω《Note 1》 @j6B / / / ?μr#DIO■/ψli.rJ/!I, / Light ＾ formation activity (Note υ binding [0 0 2 6] Note 1) At the start of the test, the fresh weight of 10 barley sprouts in each test plot was measured, and after 20 days, the fresh weight of the remaining plants in each test plot was measured. [0 0 2 7] (Note 2) On the 10th and 20th day. Two specimens were taken from the surviving individuals in each test area, and the oxygen generation rate per fresh weight (i.e., micromole oxygen/g fresh weight) was determined using the oxygen electrode method.
time) was measured. [0 0 2 8] As is clear from these results, complete prevention of lodging cannot be expected in the uniconazole 0.25 ppm plot, but the mortality rate after 20 days was nil compared to over 60% in the control plot. A second leaf appeared in about half of the surviving individuals. In addition, the fresh weight of the plants hardly decreased or even slightly increased. Furthermore, almost no decrease in photosynthetic activity was observed from the 10th day to the 20th day. From these facts, it is clear that uniconazole not only has the effects of straightening plants and preventing growth, which have been confirmed so far, but also has an effect of maintaining plant activity under low light conditions, that is, extending the life of plants. be. [0 0 2 9] In the Uniconazole 1.0pp plot, a remarkable lodging prevention effect was observed, but growth almost stopped as seen from the decrease in fresh weight, and the photosynthetic activity of surviving individuals after 20 days was also lower than that in the control plot. It has decreased to almost the same level as the surviving individuals. In the uniconazole 5.0 ppm group, the growth inhibition effect (drug damage) was noticeable. In summary, under the present experimental conditions, the concentration of uniconazole ranged from 0.1 ppm to 1.0 ppm.
oppm, especially 0.2ppm to 0.5ppm
is considered desirable. [00301 Example 2 The effect of uniconazole on prolonging the life of turf under low light conditions was compared with a non-additive control plot using Verennial ryegrass (variety: Manhattan), a cold-season Western turf, as a target plant. As uniconazole, the same product as in Example 1 was used. [00311 First, the test plots were a uniconazole-free control plot, a 0.5 ppm plot, a 1.0 ppm plot, and a 2.5 p
Four pm wards were established. The diluted solution was prepared using deionized water, and the control plot without additives was irrigated with deionized water. Three pots were prepared for each test. [0032] Put an appropriate amount of mixed soil made by mixing equal weights of field soil and river sand into a bottomed cylindrical plastic cultivation pot with a diameter of 15 cm and a depth of 12 cm (about 9 cm deep), and add the above grass seeds per pot. 1.5 g each was sown. 100 ml of each uniconazole test solution was applied to the plants, and the plants were cultivated at a temperature of 25° C. and an illuminance of 700 lux (day length of 10 hours). After germination, leaf spray treatment (approximately 5 ml) was performed once every 3 days. Hyponex (5-10-5) 500 per pot as fertilizer
The cells were irrigated with 100 ml of the double solution once a week. The grass was cut at a height of about 2.5 cm two weeks after germination, and then used for the test. [0033] For the measurement of photosynthetic activity, the test amount of leaf pieces was 10
All procedures were carried out in the same manner as in Example 1, except that the dose was 0 mg. [0034] The test results are shown in Table 2. [0035]

[Table 2] Effect of uniconazole on the early growth of perennial ryegrass "Uniconazole concentration CDTJm" Measurement items ■ No additive control 0.5 1.0 2.5 Number of days required for germination (days) Germination rate (al) Amount of elongation (cm) Report 2) [0036] (Note 1) Visual germination rate C when the control plot is set to 10
Evaluation (Note 2) As is clear from the average elongation scale 2 on the 7th day after germination, the influence of uniconazole on the number of days required for germination and the germination rate was hardly observed at the concentration of this example. . On the other hand, there was a suppressive effect on the amount of elongation, and when considering the reduction in the number of mowing operations, the favorable effect was remarkable. Cultivation was continued under such conditions, and the results of observation every week are shown in Table 3, and the photosynthetic activity of the mouth two weeks after germination is shown in Table 4. [0037]

[Table 3] Effect of uniconazole on maintaining the activity of verrenial ryegrass under low light conditions Uniconazole concentration after germination (ppm) (a) No additive control (1,5 1,0 2,5 +++ +++ +++ Now++ + ++ +++ +++ + + +++ ++ + ++ ++ + ++ ++ [0038] Evaluation criteria: ++ Healthy, ++ Slightly impaired, obviously impaired, - Dead or not worth appreciating + [0039]

[Table 4] Photosynthetic activity of verennial ryegrass grown under low light conditions Uniconazole concentration Photosynthetic activity (ppm) (μmolO/sr*hr) No additive control 23.4 0.5 26.6 1.0 39.6 2 .5 27.1 [00401 Two weeks after germination, measurements were carried out on two different pots and the average value was calculated. [0041] As is clear from Tables 3 and 4, in the control plot without the addition of uniconazole, the length was remarkable, and after germination 2
By the time the grass was mown on a weekly basis, it had become significantly weakened and its value as grass had decreased considerably. On the other hand, in the uniconazole 1.0 ppm group, efforts were still being maintained at a healthy level even after 3 weeks, and this state continued even after 5 weeks with a slight decline. Such good results were obtained for the uniconazole 2.5ppm group, which was close to the 1.0ppm group, but 1. o
It was no match for the vitality of the ppm district. [0042] From the above, for perennial ryegrass grown under low light conditions, the concentration of uniconazole is 0.5 ppm to 2.5 ppm, especially 1.0
It is clear that foliar spraying (once a week, 5 ml per pot) at around ppm has a survival effect more than twice that of the non-additive control. [0043] Example 3 The effects of uniconazole and paclobutrazol were compared with a non-additive control using current year's main branches (10 to 13 cm long) of Goldfrest and New Rose as test plants. As uniconazole, the same product as in Example 1 was used, and as paclobutrazol, Ponzai (trade name, manufactured by Takeda Pharmaceutical Company Limited) (a hydrating powder containing 2.0% paclobutrazol) was used. Each was diluted with tap water to a predetermined concentration to obtain a test solution. [0044] 200 ml of a test solution of a predetermined concentration was placed in each 500 ml glass beaker, and 5 cuttings of Goldfrest and New Rose were inserted into the beakers. ), the number of days until the tree loses its vigor, including withering or defoliation, was visually tracked. On the way, 10
Every day, the test solution was replaced with a fresh solution of the same concentration. The test results are shown in Table 5. [00451 [Table 5] Plant activity maintenance effect of triazole-based acclimation agent under low seismic intensity conditions r■■■ Plant drug concentration (%) Ratio of days until death or loss of vigor (%) No additive control 100 (Note 1) ) 0.5 Gold Uniconazole 1.0 Fresto 2.0 ? 2 (JiiF) 1.25 Paclobutrazol-free control 0.5 Neuvalauuniconazole 1.0 2.0 50 (iiiF) 1.25 Paclobutrazol [0046] (Note 1) Average days ratio of 5 repeated individuals . The control without drug addition was set at 100. [0047] As is clear from Table 5, for Goldfrest, uniconazole 0.5 ppm to 1.0
A life-prolonging effect was observed at a concentration of paclobutrazol of 5 ppm to 20 ppm. [00481 Regarding Noibara, Uniconazole has a survival effect at a concentration of 0.5 ppm to 1.0 ppm, and Paclobutrazol has a survival effect at a concentration of 1.25 ppm to 20 ppm.
Similar effects were observed across the experimental plots. [00491 From the above events, uniconazole and paclobutrazol should be used at different drug concentrations,
Although the optimal conditions may differ for control plants, such as the timing of application or the route of drug absorption (e.g., potassium absorbed by foliar spray or roots), it is clear from the results of the above examples that at least triazole-based It has been confirmed that all of the acclimatizers have a remarkable life-extending effect on plants placed under low light conditions, such as 500 lux to 1000 lux or lower, such as in indoor greening.

[Brief explanation of the drawing]

FIG. 1 is a vertical sectional view showing the cultivation state of barley in a cultivation cup.

[Procedural amendment]

[Submission date] January 29, 1991

[Procedural amendment 1]

[Name of document to be amended] Specification

[Correction target item name] 0023 [Correction method] Change

[Correction details]

[0023] Photosynthetic activity was measured by an oxygen electrode method (electrode: manufactured by Rank Brothers). i.e. 50mM HEPE containing 10mM NaHCO3
S (N-2-hydroxyethylpiperazine-N-2
-ethanesulfonic acid)-NaOH, 50.0 mg of chopped barley leaves in 4.0 ml of pH 7°2 reaction solution
After pre-irradiation for 5 minutes at a temperature of 25° C. and an illumination intensity of 20,000 lux, the oxygen generation rate was measured under the same temperature and illuminance conditions.

[Procedural amendment 2]

[Name of document to be amended] Specification

[Correction target item name] 0024 [Correction method] Change

[Correction details]

[00241 The results of the analytical test are shown in Table 1.

[Procedural amendment 3] [Name of document to be amended] Specification

[Correction target item name] 0029 [Correction method] Change

[Correction details]

[0029] In the uniconazole 1.0 ppm plot, a remarkable lodging prevention effect is observed, but as can be seen from the decrease in fresh type, growth almost ceases, and the photosynthetic activity of surviving individuals after 20 days is almost the same as that of surviving individuals in the control plot. decreased to the same level. In the uniconazole 5.0 ppm group, the growth inhibition effect (drug damage) was noticeable. In summary, under the present experimental conditions, the concentration of uniconazole was 0. lppm to 1
．． 0 ppm, especially 0.2 ppm to 0.5 ppm is considered desirable.

[Procedural amendment 4]

[Name of document to be amended] Specification

[Correction target item name] 0041 [Correction method] Change

[Correction details]

[0041] As is clear from Tables 3 and 4,
In the control plot without the addition of uniconazole, the growth was remarkable, and when pruned 2 weeks after germination, there was significant weakness.
Its value as grass had decreased considerably. On the other hand, in the uniconazole 1.0 ppm group, the animals still maintained healthy vitality even after 3 weeks, and this state continued even after 5 weeks with a slight decline. Such good results were obtained for Uniconazole 2.5ppm in a state close to that of 1.0ppm;
It was no match for the vitality of the ppm district.

Claims (2)

[Claims] 1. An agent for maintaining plant activity under low light conditions, which contains a compound having a triazole group as an active ingredient. Claim 2: Paclobutrazol [(2RS-3RS)
-1-(4-chlorophenyl)-4,4-dimethyl-2
-(1H-1,2,4-triazol-1-yl)pentan-3-ol] or uniconazole [(E)-(
RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)-1-penten-3-ol] as an active ingredient. An agent for maintaining plant activity under low light conditions.