JPH04187610A - Cosmetics - Google Patents

Abstract

PURPOSE:To obtain a cosmetic preventing aging of skin and stabilizing protein in cosmetic by blending a cosmetic base with carnosine, anserine, balenine or an acid addition salt thereof having excellent antioxidation effect against oxidative reaction of protein. CONSTITUTION:A cosmetic base is blended with carnosine (beta-alanine-L-histidine), anserine (beta-alanyl-L-1-methylhistidine), balenine (beta-alanyl-L-3-methylhistidine) or an acid addition salt thereof to give the cosmetic. A basic cosmetic such as toilet lotion, milky lotion, cream, lotion or powder and the like and make-up cosmetic such as lipstick, hair tonic, hair spray, hair cream, face washing cream, shampoo may be cited as the cosmetic. The cosmetic increases elasticity and flexibility of skin and is effective for preventing chapped skin, fine wrinkles, fishskin, stain, freckle.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a cosmetic containing carnosine, anserine, balenine, or an acid adduct thereof as an active ingredient, which has an antioxidant effect.

[Conventional technology] Japan's average life expectancy has shown remarkable growth year by year, and has now become one of the top in the world. Now that we have an aging society, there is a desire to stay healthy and young. However, as people grow older, their organs and organs begin to age in some way.

The skin is the outermost layer of the body, and it protects the living body from foreign substances and stimuli from the outside, and at the same time has various physiological functions such as regulating body temperature and sensory functions.However, like other organs, it begins to age at the age of one year. progresses, loses elasticity and flexibility,
It becomes hard and eventually wrinkled. However, women have a strong desire to maintain beautiful skin.

The skin has an epidermis on the outside and a dermis on the inside of the epidermis. Epidermal cells form the multilayered structure of the epidermis to survive, proliferate,
The dermis is necessary for differentiation. The dermis consists primarily of collagen and contains fibroblasts that synthesize collagen.

Age-related changes in connective tissue such as the skin are said to be due to changes in collagen, a central component of connective tissue, especially changes in its cross-links. When we are young, we can form Schiff base crosslinks and pyridinoline crosslinks, which are important for our bodies, but as we get older,
Unfavorable cross-links such as histidinoalanine are formed, and this is thought to be the cause of connective tissue becoming stiffer with aging.

Such loss links are thought to be generated by protein oxidation reactions. The process by which Schiff base-type cross-links change to stable or loss-links such as pyridinoline is also an oxidation reaction, so antioxidants are said to be effective in preventing this change.

On the other hand, when ascorbate is added to a proteinaceous material such as collagen or a multilayer material such as hyaluronic acid in cosmetics, ascorbic acid, which should have been added to prevent oxidation, is present in the presence of trace amounts of metals. This generates hydrogen peroxide and causes oxidation. This oxidation effect may cause the polymer to become lower in molecular weight, resulting in a significant deterioration in quality. Synthetic antioxidants are used to prevent this, but there are many safety issues. Therefore, alternatives to these substances are desired.

When a protein undergoes oxidation, its molecules break down or
On the contrary, it causes polymerization. It has also been reported that among its constituent amino acids, histidine and tryptophan are specifically damaged, resulting in an increase in the amount of aldehyde groups within the protein molecule. It is believed that damage caused by such oxidative reactions accelerates or triggers aging in cells and tissues. Therefore, if we can prevent such protein oxidation reactions, we can prevent skin aging, increase skin elasticity and flexibility, and reduce rough skin, fine lines, shark skin, age spots, and freckles. It is possible to provide cosmetics that are also effective for prevention, which is extremely meaningful.

[The invention attempts to solve the problem] D Based on these actual circumstances, the inventors have developed a highly safe and
As a result of extensive research aimed at providing highly effective cosmetics, we discovered that carnosine, anserine, and balenine have excellent antioxidant effects against protein oxidation reactions. By using these compounds, we have found a method useful for preventing skin aging and stabilizing proteins in cosmetics, and have completed the present invention.

[Means for Solving the Problems] The gist of the present invention is to use carnosine, anserine, balenine, or an acid addition salt thereof as an active ingredient.

Here, the acid addition salts include pharmaceutically acceptable salts such as hydrochloride, sulfate, nitrate! ! Preferred examples include salts, succinates, citrates, tartrates, and the like.

Carnosine (β-alanyl-L-histidine), anserine (β-alanyl-L-1-methylhistidine), and balenine (β-azinyl-L-3-methylhistidine) are found in m+ mammals, birds, reptiles, and amphibians. It is a dipeptide that exists in muscle tissue such as, and is already a known substance. Since the discovery of these peptides at the beginning of this century, many studies have been conducted, and it has been reported that carnosine and anserine exist in the skeletal muscle of vertebrates at a concentration range of 1 to 20mM, and the content varies depending on the type of muscle. and changes with the age of the animal. Substances such as carnosine, anserine, and balenine are thought to play some physiological role in muscles and the brain, but there is still no theory that can fully explain their role. These substances are said to act as neurotransmitters and as buffers to neutralize lactic acid produced in muscles through anaerobic glycolysis, but this is not clear. do not have.

However, when the inventors conducted experiments on the antioxidant effects of carnosine, anserine, and valenine on proteins, they discovered that they had unexpectedly excellent effects.

Since these substances originally exist in living organisms, they are considered to be of low toxicity and high safety, and have good solubility in water, so they are considered to have great significance as antioxidants.

Carnosine, anserine, and balenine used in the present invention are extracted from natural products 1, such as the broth discharged during the production of bonito flakes or dried sardines, the broth discharged during the production of canned tuna, or extracted from inexpensive raw materials such as chicken meat. A chemically synthesized product or an enzymatically synthesized product can also be used.

In the present invention, when carnosine, anserine, and balenine are used in cosmetics for antioxidant purposes, carnosine, anserine, and balenine may be used alone or in combination, but other substances known to have antioxidant effects may also be used. May be used in combination with antioxidants and synergists.

As antioxidants, antioxidants such as BHA and BHT are also used.
Examples of synergists include ascorbic acid, citric acid, phosphate, and phytic acid.

In addition, carnosine, anserine, and balenine are used in commonly accepted carriers (excipients, lubricants, binders, colorants, emulsifiers,
It is blended into cosmetics according to conventional methods along with fragrances, etc.).

The form of cosmetics in the present invention includes powder, semi-solid,
This includes all forms of cosmetics, such as paste or liquid.

Cosmetics include basic cosmetics such as lotions, milky lotions, creams, lotions, and powders, makeup amplifier cosmetics such as lipsticks, hair tonics, hair sprays, hair creams, facial cleansing creams, shampoos, and the like.

As a method for producing these cosmetics, conventional methods for producing cosmetics can be used. In addition, the blending amount varies depending on the morphological properties of the cosmetic, but generally o, 0
It is preferably 1 to 50%, but is not particularly limited.

[Examples] The present invention will be explained below using experimental examples and examples, but these do not limit the present invention.

Experimental example 1 β-carotene 40mg + linoleic acid 40mg,
After dissolving Twin 40400B in a small amount of chloroform, remove the chloroform using a rotary evaporator.
Dry under reduced pressure. This is water with oxygen gas aerated in it for 10 minutes.
0ml was added to prepare an emulsion solution for 1s.

To this emulsion solution 9i1, 1 ml of sample solutions of various concentrations were added to prepare a test solution.

The test solution was left in a constant temperature bath at 40°C, and 0.5ml of the solution was taken out at regular intervals, 9.5ml of ethanol was added, and the mixture was thoroughly mixed.The absorbance at a wavelength of 450nm was measured to determine the antioxidant effect of carnosine. I experimented. The results are shown in the table below.

0 minutes 60 minutes 120 minutes 240 minutes control group 0
．． 495 0.2g4 0.205 0.1331mM
O, 4950, 2980, 2270, 1805mM
O, 4950, 3410, 3010, 23710m
M O, 4950, 3600, 3250, 25650
mMO, 4950, 3730, 3330, 2991
00+nM O, 4950, 3440, 3170, 2
As the amount of 84 carnosine increased, the decomposition of β-carotene was suppressed, and an antioxidant effect was observed.

Experimental Example 2 Bovine serum albumin 0.0 was added to a mixture of 17.5 ml of 0.1 M phosphate buffer and 2.5 ml of 0.1 M steel sulfate solution.
Add 1g to 20ml of protein sample solution prepared by dissolving various amino acids etc. to a final concentration of 10mM.
After adding 5 ml of 0 mM sodium ascorbate solution and mixing well, ascorbic acid was reacted with bovine serum albumin at room temperature in the presence of copper for 24 hours. After reaction 1 reaction solution 4.
5ml was collected, and 0.5Ill of 0.4mM tetrasodium ethylenediaminetetrabutyrate solution was added as a reaction stopper to stop one reaction.

Next, 5 ml of electrophoresis sample preparation solution was added to this solution, and then 5 ml of 8M urea was added to prepare a sample for electrophoresis. This sample solution was electrophoresed using a gradient gel manufactured by Daiichi Kagaku ■. After electrophoresis, staining was performed using Kumasi Brilliant Blue R-250. In order to examine the effect of carnosine on protein oxidation, the concentration of the main band of bovine serum albumin was measured using a chromato scanner C8-9000 manufactured by Shinsei Seisakusho.

The antioxidant effect was compared by comparing the area on the chart and the maximum absorbance. The results are shown in the table below.

Area Absorbance control area 159441 0.88 Carnosine 206723 1J3 Histidine
204437 1.69 Threonine
157626 1.12 Methionine
150894 1.15 Phenylalanine 167
301 1.21 Tryptophan 170084
1.29 serine 171440 1
．． 40 Lysine 166665 1.3
5 Arginine 185583 1.13 Cysteine Hydrochloride 196959 1.35 Carnosine and histidine were found to have excellent antioxidant effects, but since histidine causes significant coloring of the reaction solution, carnosine is preferable to histidine.

Experimental Example 3 An experiment similar to Experimental Example 2 was conducted by changing the concentration of carnosine in one reaction solution, and changes in the antioxidant effect were investigated.

Area Absorbance Unreacted area 100616 1.43 No addition area 60060 0.81 Carnosine 1mM 85631 1.19 Carnosine 5mM 90237 1.17 Carnosine 110l11 110192 1.44 Lunosine 50mM 112233 1.46
Force Lunosine 100mM 107079 1.
The oxidation was suppressed as the amount of 44 carnosine added increased, but the effect was almost the same at 10 mM or more.

Experimental Example 4 Among the protein sample solutions of Experimental Example 2, the protein was replaced with β-lactoglobulin from bovine serum albumin, and
A similar experiment was conducted using a peptide concentration of 100N. The results are shown in the table below.

Absorbance diagram without additives 0.22 Carnosine 1.08 Anserine 1.16 Histidine 1.14 GlrGly-His 1.06 β-alanine 0.77 Example 1 2.0 parts of propylene glycol, 0.5 parts of polyoxyethylene castor oil derivative , carnosine 0.1 part, oxalic acid 0
．． A lotion was obtained by mixing 97.09 parts of purified water with 0.01 part of sand, 0.01 part of methyl paraoxybenzoate, and 0.2 part of methyl paraoxybenzoate.

Example 2 Seranol 5.0 parts, lanolin 2.5 parts, solid paraffin 4.0 parts, liquid paraffin 22.0 parts, lipophilic surfactant 2.0 parts, hydrophilic surfactant 3.5 parts, preservative Agent 0.
5 parts of carnosine, 0.3 parts of BHA O, 1 part of BHA O, 10.0 parts of glycerin, and 49.9 parts of purified water were heated and dissolved at 80°C, mixed and emulsified, and 0.2 parts of fragrance was added while cooling. The mixture was added and uniformly dispersed to obtain a cream.

Example 8 3.0 parts of beeswax, 5.0 parts of isopropyl myristate, 13.0 parts of liquid paraffin, 1.5 parts of lipophilic surfactant
part, hydrophilic surfactant 3.0 parts, carnosine 0.2 parts,
0.1 part of anserine, 0.3 part of preservative, 7.0 parts of propylene glycol, and 66.7 parts of 1fiU water were heated and dissolved at 80°C, mixed and emulsified, and 0.2 part of fragrance was added while cooling. The mixture was uniformly dispersed to obtain a milky lotion.

Claims (1)

[Claims] (1) A cosmetic comprising carnosine, anserine, balenine, or an acid addition salt thereof in a cosmetic base.