JPH0416218A - Bactericidal filtration membrane - Google Patents
Bactericidal filtration membraneInfo
- Publication number
- JPH0416218A JPH0416218A JP12036390A JP12036390A JPH0416218A JP H0416218 A JPH0416218 A JP H0416218A JP 12036390 A JP12036390 A JP 12036390A JP 12036390 A JP12036390 A JP 12036390A JP H0416218 A JPH0416218 A JP H0416218A
- Authority
- JP
- Japan
- Prior art keywords
- membrane
- cephalosporin
- precision
- bacteria
- filtration membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012528 membrane Substances 0.000 title claims abstract description 34
- 230000000844 anti-bacterial effect Effects 0.000 title claims description 7
- 238000001914 filtration Methods 0.000 title claims 3
- HOKIDJSKDBPKTQ-GLXFQSAKSA-N cephalosporin C Chemical compound S1CC(COC(=O)C)=C(C(O)=O)N2C(=O)[C@@H](NC(=O)CCC[C@@H](N)C(O)=O)[C@@H]12 HOKIDJSKDBPKTQ-GLXFQSAKSA-N 0.000 claims abstract description 30
- 239000011347 resin Substances 0.000 claims abstract description 6
- 229920005989 resin Polymers 0.000 claims abstract description 6
- 241000894006 Bacteria Species 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 3
- 238000001471 micro-filtration Methods 0.000 abstract 3
- YVXDRFYHWWPSOA-BQYQJAHWSA-N 1-methyl-4-[(e)-2-phenylethenyl]pyridin-1-ium Chemical group C1=C[N+](C)=CC=C1\C=C\C1=CC=CC=C1 YVXDRFYHWWPSOA-BQYQJAHWSA-N 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 229930186147 Cephalosporin Natural products 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 229920002301 cellulose acetate Polymers 0.000 description 2
- 229940124587 cephalosporin Drugs 0.000 description 2
- 150000001780 cephalosporins Chemical class 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 2
- 229920002554 vinyl polymer Polymers 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- -1 for example Polymers 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000004260 plant-type cell wall biogenesis Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、殺菌性口過膜に関する。更に詳しくは、精密
口過膜表面における細菌の増殖を阻止せしめる殺菌性口
過膜に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a bactericidal oral membrane. More specifically, the present invention relates to a bactericidal oral membrane that inhibits the growth of bacteria on the surface of a precision oral membrane.
〔従来の技術〕および〔発明が解決しようとする課題〕
酢酸セルロース多孔質膜によって代表される、孔径的０
．２μ−以下の精密口過膜を用い、大気中の細菌を口過
し、浄化する方法が従来から採用されている。しかしな
がら、この方法では、１００％細菌を阻止することは技
術的にも困難であった。それは、阻止された細菌が膜表
面で増殖し、遂には膜の片側に抜けてしまうことが一因
とされている。[Prior art] and [Problem to be solved by the invention]
Pore size 0 represented by cellulose acetate porous membrane
．． Conventionally, a method has been adopted in which bacteria in the atmosphere are filtered and purified using a precision filter membrane with a diameter of 2μ or less. However, it was technically difficult to inhibit bacteria 100% with this method. One reason for this is thought to be that the blocked bacteria proliferate on the membrane surface and eventually escape to one side of the membrane.
そこで、精密口過膜表面での細菌の増殖を抑制すること
が強く要望されている。Therefore, it is strongly desired to suppress the growth of bacteria on the surface of the precision membrane.
本発明の目的は、精密口過膜表面における細菌の増殖を
阻止せしめる殺菌性口過膜を提供することにある。An object of the present invention is to provide a bactericidal oral membrane that inhibits the growth of bacteria on the surface of the precision oral membrane.
〔課題を解決するための手段〕および〔作用〕かかる本
発明の目的は、精密口過膜表面にセファロスポリンＣ含
有水溶性光架橋性樹脂の光架橋膜を形成せしめた殺菌性
口過膜によって達成される。[Means for Solving the Problem] and [Action] The object of the present invention is to provide a bactericidal oral membrane in which a photocrosslinked film of a water-soluble photocrosslinkable resin containing cephalosporin C is formed on the surface of a precision oral membrane. achieved by
セファロスポリンＣは、 Ｃｅｐｈａｌｏｓｐｏｒｉｕ
ｍの産生する抗菌性抗生物質であり、ペニシリンと同し
く抗菌作用は細菌細胞の細胞壁合成の阻害にあるとされ
ており、本発明においては、かかる作用を有するセファ
ロスポリンＣを精密口過膜表面に固定化せしめることに
より、膜表面における細菌の増殖を阻止せんとするもの
である。Cephalosporin C is Cephalosporin C.
Like penicillin, its antibacterial effect is said to be due to the inhibition of cell wall synthesis of bacterial cells.In the present invention, cephalosporin C, which has such an effect, is applied to a precision oral membrane. By immobilizing it on the surface, it is intended to prevent the growth of bacteria on the membrane surface.
精密口過膜表面へのセファロスポリンＣの固定化は、水
溶性光架橋性樹脂の水溶液中にセファロスポリンＣを混
合し、それを精密口過膜表面に塗布し、乾燥させた後、
紫外線照射して光架橋膜を形成させることにより行われ
る。Immobilization of cephalosporin C on the surface of the precision membrane is carried out by mixing cephalosporin C in an aqueous solution of a water-soluble photocrosslinkable resin, applying it to the surface of the precision membrane, and drying it.
This is done by irradiating ultraviolet rays to form a photocrosslinked film.
水溶性光架橋性樹脂としては５例えば分子中に光架橋性
基としてスチルバゾリウム基、ジアゾ基などの感光性基
、好ましくはスチルバゾリウム基を有するポリビニルア
ルコール、ポリエチレングリコールなどが用いられる。As the water-soluble photocrosslinkable resin, for example, polyvinyl alcohol, polyethylene glycol, and the like having a photosensitive group such as a stilbazolium group or a diazo group as a photocrosslinkable group in the molecule, preferably a stilbazolium group, are used.
セファロスポリンＣは、このような水溶性光架橋性樹脂
の約０．１〜５重量％水溶液中に約０．０５〜１５重量
石の濃度で混入し、この溶液が精密口過膜に塗布される
。塗布方法には特に制限がないが、般的には浸漬法が用
いられる。その後、室温下で約１〜３時間程乾燥し、次
いで波長３０００〜４５００人の紫外線を約５〜６０秒
間照射し、形成された光架橋膜中にセファロスポリンＣ
を固定化させる。Cephalosporin C is mixed into an aqueous solution of about 0.1 to 5% by weight of such water-soluble photocrosslinkable resin at a concentration of about 0.05 to 15% by weight, and this solution is applied to the precision oral membrane. be done. Although there are no particular restrictions on the coating method, a dipping method is generally used. Thereafter, it is dried at room temperature for about 1 to 3 hours, and then irradiated with ultraviolet rays with a wavelength of 3,000 to 4,500 people for about 5 to 60 seconds to form a photocrosslinked film containing cephalosporin C.
to be fixed.
〔発明の効果〕
このようにして精密口過膜の表面上に光架橋膜を介して
包括固定化されたセファロスポリンＣは、細菌に対して
膜表面でのそれの増殖を有効に阻止する働きを有してい
る。[Effects of the Invention] Cephalosporin C, which is thus comprehensively immobilized on the surface of the precision oral membrane via the photocrosslinked membrane, effectively inhibits bacteria from multiplying on the membrane surface. It has a function.
次に、実施例について本発明を説明する。 Next, the present invention will be explained with reference to examples.
実施例
酢酸セルロース製精密口過膜（孔径０．２μＬ厚さ０．
２ｍｍ）を、水溶性光架橋性ポリビニルアルコール（光
架橋性スチルバゾリウム基含有量１．４モル％。Example Precision membrane made of cellulose acetate (pore size: 0.2 μL, thickness: 0.2 μL)
2 mm) and water-soluble photocrosslinkable polyvinyl alcohol (photocrosslinkable stilbazolium group content: 1.4 mol%).
けん化度８８％、重合度１４００）の１重量％水溶液中
にセファロスポリンＣを０．５重量％混入した溶液中に
１分間浸漬し、室温下で２時間乾燥させた後、波長４０
００人の紫外線を各面３０秒間ずつ表裏両面に照射した
。After soaking for 1 minute in a solution containing 0.5% by weight of cephalosporin C in a 1% by weight aqueous solution with a degree of saponification of 88% and a degree of polymerization of 1400), and drying at room temperature for 2 hours,
Both the front and back sides were irradiated with ultraviolet light from 00 people for 30 seconds on each side.
このようにして得られたセファロスポリンＣ固定化精密
口過膜（直径４ｃｍ）を、クリーンベンチ内に設置した
ビニル管の途中にセルを介して固定し、ビニル管の一端
側を大気中に開放とし、他端側をクリーンベンチ外に設
置したポンプに連結して吸引した。The thus obtained cephalosporin C-immobilized precision membrane (diameter 4 cm) was fixed through a cell in the middle of a vinyl tube installed in a clean bench, and one end of the vinyl tube was exposed to the atmosphere. It was left open, and the other end was connected to a pump installed outside the clean bench for suction.
吸引を１時間続けた後、セファロスポリンＣ固定化精密
口過膜をはずし、それをシャーレに入れ。After continuing suction for 1 hour, the cephalosporin C-immobilized precision membrane was removed and placed in a petri dish.
その上側からブイヨン培地（リービーヒ肉エキス７．５
ｇ、ペプトン（Ｏｇ、 Ｖ；＋ＣＱ ５ｇち゛よび寒
天１５ｇ 全溶解；ρ１１７ 、０ ）を重層し、３７
℃で４８時間培養したが、細菌のコロニーは見られなか
った。なお、使用した試薬、器具なとは、ポンプ以外す
べて滅菌処理済みのものを使用した。From the top, bouillon medium (Liebee meat extract 7.5
g, peptone (Og, V; +CQ 5g and agar 15g, completely dissolved; ρ117,0) were layered, 37
Although cultured at ℃ for 48 hours, no bacterial colonies were observed. All reagents and instruments used, except for the pump, were sterilized.
比較例
上記実施例において、セファロスポリンＣを用いずに、
単なる光架橋膜を精密口過膜表面に形成させたものにつ
いて培養を行ったところ、無数のコロニーが観察された
。Comparative Example In the above example, without using cephalosporin C,
When a simple photo-crosslinked film was formed on the surface of a precision oral membrane and cultured, numerous colonies were observed.
Claims (1)
光架橋性樹脂の光架橋膜を形成せしめてなる殺菌性ロ過
膜。1. A bactericidal filtration membrane formed by forming a photocrosslinked film of a water-soluble photocrosslinkable resin containing cephalosporin C on the surface of the precision filtration membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12036390A JPH0416218A (en) | 1990-05-10 | 1990-05-10 | Bactericidal filtration membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12036390A JPH0416218A (en) | 1990-05-10 | 1990-05-10 | Bactericidal filtration membrane |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0416218A true JPH0416218A (en) | 1992-01-21 |
Family
ID=14784346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12036390A Pending JPH0416218A (en) | 1990-05-10 | 1990-05-10 | Bactericidal filtration membrane |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0416218A (en) |
-
1990
- 1990-05-10 JP JP12036390A patent/JPH0416218A/en active Pending
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