JP2990786B2 - Antimicrobial membrane - Google Patents

Antimicrobial membrane

Info

Publication number
JP2990786B2
JP2990786B2 JP2293976A JP29397690A JP2990786B2 JP 2990786 B2 JP2990786 B2 JP 2990786B2 JP 2293976 A JP2293976 A JP 2293976A JP 29397690 A JP29397690 A JP 29397690A JP 2990786 B2 JP2990786 B2 JP 2990786B2
Authority
JP
Japan
Prior art keywords
membrane
avidin
microorganisms
immobilized
filtration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2293976A
Other languages
Japanese (ja)
Other versions
JPH04166221A (en
Inventor
正男 後藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nok Corp
Original Assignee
Nok Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nok Corp filed Critical Nok Corp
Priority to JP2293976A priority Critical patent/JP2990786B2/en
Publication of JPH04166221A publication Critical patent/JPH04166221A/en
Application granted granted Critical
Publication of JP2990786B2 publication Critical patent/JP2990786B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3823—Affinity chromatography of other types, e.g. avidin, streptavidin, biotin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/12—Composite membranes; Ultra-thin membranes
    • B01D69/125—In situ manufacturing by polymerisation, polycondensation, cross-linking or chemical reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/48—Antimicrobial properties

Description

【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、抗微生物膜に関する。更に詳しくは、ろ過
膜に抗微生物特性を付与した抗微生物膜に関する。The present invention relates to an antimicrobial membrane. More specifically, the present invention relates to an antimicrobial membrane having antimicrobial properties imparted to a filtration membrane.

〔従来の技術〕および〔発明が解決しようとする課題〕 酢酸セルロース多孔質膜によって代表される、孔径約
0.2μｍ以下の精密ろ過膜を用い、大気中および溶液中
の微生物をろ過し、浄化する方法が従来から採用されて
いる。しかしながら、この方法では、100％微生物を阻
止することは技術的にも困難であった。それは、阻止さ
れた微生物が膜表面で増殖し、遂には膜の片側に抜けて
しまうことが一因とされている。そこで、ろ過膜表面で
の微生物の増殖を抑制することが強く要望されている。[Prior Art] and [Problems to be Solved by the Invention] Pore size represented by a cellulose acetate porous membrane
A method of filtering and purifying microorganisms in the atmosphere and in a solution using a microfiltration membrane of 0.2 μm or less has been conventionally employed. However, in this method, it was technically difficult to control 100% of microorganisms. It is attributed in part to the arrested microorganisms growing on the membrane surface and eventually escaping to one side of the membrane. Therefore, there is a strong demand for suppressing the growth of microorganisms on the surface of the filtration membrane.

その対策としては、ろ過膜表面に抗生物質を固定化す
る方法もあるが、抗生物質は分子量が小さいため、膜か
ら抜け落ちてしまう欠点がみられる。As a countermeasure, there is a method of immobilizing an antibiotic on the surface of a filtration membrane. However, since the antibiotic has a small molecular weight, there is a drawback that the antibiotic falls off the membrane.

本発明の目的は、ろ過膜面上における微生物の増殖を
阻止せしめる抗微生物膜を提供することにある。An object of the present invention is to provide an antimicrobial membrane that inhibits the growth of microorganisms on the surface of a filtration membrane.

〔課題を解決するための手段〕および〔作用〕 かかる本発明の目的は、ろ過膜面上にアビジンを固定
化せしめた抗微生物膜によって達成される。[Means for Solving the Problems] and [Action] The object of the present invention is achieved by an antimicrobial membrane having avidin immobilized on a filtration membrane surface.

アビジンは、鶏卵の卵白中に含まれ、ビオチンと特異
的に結合する分子量約6600の糖たん白質である。そし
て、微生物の増殖に必要なビオチン（ビタミンＨ、補酵
素）の２モルと選択的に結合し、これを不活性化するこ
とにより、微生物の増殖を阻害する作用を有している。
即ち、アビジン−ビオチン複合体はきわめて安定（解離
定数は10-15M）で、ビオチンはビタミンとしての活性な
らびにビオチン酵素の補酵素としての活性を失うように
なる。Avidin is a glycoprotein with a molecular weight of about 6600 that is specifically contained in egg white of chicken eggs and specifically binds to biotin. Then, it selectively binds to 2 moles of biotin (vitamin H, coenzyme) necessary for the growth of microorganisms and inactivates them, thereby inhibiting the growth of microorganisms.
That is, the avidin-biotin complex is extremely stable (dissociation constant is 10 −15 M), and biotin loses its activity as a vitamin and as a coenzyme of a biotin enzyme.

本発明においては、かかる作用を有するアビジンをろ
過膜の表面および内部に固定化せしめることにより、膜
面上における微生物の増殖を阻止せんとするものであ
る。In the present invention, the growth of microorganisms on the membrane surface is prevented by immobilizing avidin having such an action on the surface and inside of the filtration membrane.

ろ過膜としては、酢酸セルロース、ポリスルホン、ポ
リウレタン、ポリテトラフルオロエチレン、ポリカーボ
ネート、キトサンなどから成形された精密ろ過膜、限外
ろ過膜、更には孔径がÅオーダーの気体分離膜などが用
いられる。As the filtration membrane, a microfiltration membrane formed from cellulose acetate, polysulfone, polyurethane, polytetrafluoroethylene, polycarbonate, chitosan, or the like, an ultrafiltration membrane, and a gas separation membrane having a pore size on the order of Å are used.

ろ過膜面上へのアビジンの固定化は、一般には水溶性
光架橋性樹脂の水溶液中にアビジンを混合し、それをろ
過膜面上に塗布し、乾燥させた後、紫外線照射して光架
橋膜を形成させることにより行われる。Avidin is generally immobilized on the filtration membrane surface by mixing avidin in an aqueous solution of a water-soluble photocrosslinkable resin, applying the mixture on the filtration membrane surface, drying, and then irradiating with ultraviolet light to perform photocrosslinking. This is performed by forming a film.

水溶性光架橋性樹脂としては、例えば分子中に光架橋
性基としてスチルバゾリウム基、ジアゾ基などの感光性
基、好ましくはスチルバゾリウム基を有するポリビニル
アルコール、ポリエチレングリコールなどが用いられ
る。As the water-soluble photocrosslinkable resin, for example, polyvinyl alcohol, polyethylene glycol, or the like having a photosensitive group such as a stilbazolium group or a diazo group as a photocrosslinkable group in the molecule, preferably a stilbazolium group is used.

アビジンは、このような水溶性光架橋性樹脂の約0.1
〜５重量％水溶液中に約0.05〜15重量％の濃度で混入
し、この溶液がろ過膜に塗布される。塗布方法には特に
制限がないが、一般的には浸漬法が用いられる。その
後、室温下で約１〜３時間程乾燥し、次いで波長3000〜
4500Åの紫外線を約５〜60秒間照射し、形成された光架
橋膜中にアビジンを固定化させる。Avidin is about 0.1% of such a water-soluble photocrosslinkable resin.
It is mixed in a ５5% by weight aqueous solution at a concentration of about 0.05-15% by weight, and this solution is applied to a filtration membrane. Although there is no particular limitation on the application method, an immersion method is generally used. After that, it is dried at room temperature for about 1 to 3 hours,
Irradiation with ultraviolet rays at 4500 ° for about 5 to 60 seconds immobilizes avidin in the formed photocrosslinked film.

こうした包括法による固定化法以外にも、例えばアミ
ノ基を有するキトサンろ過膜などの場合には、グルタル
アルデヒドなどを用いる共有結合法によってもアビジン
を固定化することができる。In addition to the immobilization method by such an entrapment method, for example, in the case of a chitosan filtration membrane having an amino group, avidin can be immobilized by a covalent bonding method using glutaraldehyde or the like.

〔発明の効果〕〔The invention's effect〕

このようにしてろ過膜面上に固定化されたアビジン
は、微生物に対して膜の表面および内部でのそれの増殖
を有効に阻止する働きを有している。Avidin immobilized on the filtration membrane surface in this manner has a function of effectively preventing microorganisms from growing on and around the membrane.

〔実施例〕〔Example〕

次に、実施例について本発明を説明する。 Next, the present invention will be described with reference to examples.

実施例１ 酢酸セルロース製精密ろ過膜（孔径0.2μｍ、厚さ0.2
mm）を、水溶性光架橋性ポリビニルアルコール（光架橋
性スチルバゾリウム基含有量1.4モル％、けん化度88
％、重合度1400）の１重量％水溶液中にアビジン（シグ
マ社製品）を0.5重量％混入した溶液中に１分間浸漬
し、室温下で２時間乾燥させた後、波長4000Åの紫外線
を各面30秒間ずつ表裏両面に照射した。Example 1 Cellulose acetate microfiltration membrane (pore size 0.2 μm, thickness 0.2
mm) with a water-soluble photocrosslinkable polyvinyl alcohol (photocrosslinkable stilbazolium group content 1.4 mol%, saponification degree 88
%, Polymerization degree 1400) in a 1% by weight aqueous solution containing 0.5% by weight of avidin (Sigma) and dried for 2 hours at room temperature. Irradiation was performed on both front and back sides for 30 seconds.

このようにして得られたアビジン固定化精密ろ過膜
（直径4cm）を、クリーンベンチ内に設置したビニル管
の途中にセルを介して固定し、ビニル管の一端側を大気
中に開放とし、他端側をクリーンベンチ外に設置したポ
ンプに連結して吸引した。The avidin-immobilized microfiltration membrane (4 cm in diameter) obtained in this way was fixed via a cell in the middle of a vinyl tube installed in a clean bench, and one end of the vinyl tube was opened to the atmosphere. The end was connected to a pump installed outside the clean bench and suctioned.

吸引を１時間続けた後、アビジン固定化精密ろ過膜を
はずし、それをシャーレに入れ、その上側からブイヨン
培地（リービーヒ肉エキス7.5g、ペプトン10g、NaCl5g
および寒天15gを溶解;pH7.0）を重層し、37℃で48時間
培養したが、微生物のコロニーは見られなかった。な
お、使用した試薬、器具などは、ポンプ以外すべて滅菌
処理済みのものを使用した。After the suction was continued for 1 hour, the avidin-immobilized microfiltration membrane was removed, and the membrane was placed in a petri dish, and a bouillon medium (7.5 g of Liebi meat extract, 10 g of peptone, 5 g of NaCl) was placed from above.
And 15 g of agar were dissolved; pH 7.0) was overlaid and cultured at 37 ° C. for 48 hours, but no colonies of microorganisms were found. The reagents and instruments used were all sterilized except for the pump.

比較例 上記実施例１において、アビジンを用いずに、単なる
光架橋膜を精密ろ過膜表面に形成させたものについて培
養を行ったところ、無数のコロニーが観察された。Comparative Example In Example 1, when a simple photocrosslinking membrane was formed on the surface of the microfiltration membrane without using avidin, the culture was performed. As a result, countless colonies were observed.

実施例２ 実施例１のアビジン固定化精密ろ過膜を、クリーンベ
ンチ内で、水道水を１ろ過した後、実施例１と同様の
方法で培養したが、微生物のコロニーはみられなかっ
た。Example 2 The avidin-immobilized microfiltration membrane of Example 1 was subjected to one filtration of tap water in a clean bench, and then cultured in the same manner as in Example 1, but no colonies of microorganisms were observed.

実施例３ 実施例１のアビジン固定化精密ろ過膜を、クリーンベ
ンチ内で、１日１回水道水を１ろ過する操作を５日間
くり返した後、実施例１と同様の方法で培養したが、微
生物のコロニーはみられなかった。Example 3 The avidin-immobilized microfiltration membrane of Example 1 was cultured in the same manner as in Example 1 after repeating the operation of once filtering tap water once a day for 5 days in a clean bench. No microbial colonies were found.

実施例４ キトサン製精密ろ過膜（孔径0.1μｍ、厚さ0.2mm）
を、0.5％アビジン水溶液中に、４℃で１時間浸漬した
後、引き上げて25℃で１時間風乾した。次に、これを２
％グルタルアルデヒド水溶液中に10分間浸漬し、水洗し
た。Example 4 Chitosan microfiltration membrane (pore diameter 0.1 μm, thickness 0.2 mm)
Was immersed in a 0.5% avidin aqueous solution at 4 ° C. for 1 hour, pulled up, and air-dried at 25 ° C. for 1 hour. Next, this is 2
% Glutaraldehyde aqueous solution for 10 minutes and washed with water.

このようにしてアビジンを固定化させた精密ろ過膜に
ついて、実施例１と同様の大気透過および実施例２と同
様の水道水ろ過をそれぞれ実施し、培養評価したが、い
ずれも微生物のコロニーはみられなかった。The microfiltration membrane on which avidin was immobilized in this manner was subjected to the same atmospheric permeation as in Example 1 and the same tap water filtration as in Example 2, and the culture was evaluated. I couldn't.

───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.6，ＤＢ名) B01D 61/00 - 71/82 ＷＰＩ（ＤＩＡＬＯＧ)──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int. Cl. 6 , DB name) B01D 61/00-71/82 WPI (DIALOG)

Claims (1)

(57)【特許請求の範囲】(57) [Claims] 【請求項１】ろ過膜面上にアビジンを固定化してなる抗
微生物膜。1. An antimicrobial membrane comprising avidin immobilized on a filtration membrane surface.
JP2293976A 1990-10-31 1990-10-31 Antimicrobial membrane Expired - Fee Related JP2990786B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2293976A JP2990786B2 (en) 1990-10-31 1990-10-31 Antimicrobial membrane

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2293976A JP2990786B2 (en) 1990-10-31 1990-10-31 Antimicrobial membrane

Publications (2)

Publication Number Publication Date
JPH04166221A JPH04166221A (en) 1992-06-12
JP2990786B2 true JP2990786B2 (en) 1999-12-13

Family

ID=17801633

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2293976A Expired - Fee Related JP2990786B2 (en) 1990-10-31 1990-10-31 Antimicrobial membrane

Country Status (1)

Country Link
JP (1) JP2990786B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170001580A (en) * 2015-06-26 2017-01-04 고려대학교 산학협력단 3D-mesh structure and impeller having 3D-mesh structure

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5855788A (en) * 1996-02-07 1999-01-05 Kimberly-Clark Worldwide, Inc. Chemically charged-modified filter for removing particles from a liquid and method thereof
EP2576029A4 (en) * 2010-06-01 2015-10-28 3M Innovative Properties Co Coated porous materials
CN108310983B (en) * 2018-02-02 2021-02-02 山西大学 Preparation and regeneration method of antibacterial and anti-pollution PVDF ultrafiltration membrane
CN114377554B (en) * 2022-01-11 2023-04-28 浙江工业大学 Preparation method of antibacterial polytetrafluoroethylene air filtering membrane

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170001580A (en) * 2015-06-26 2017-01-04 고려대학교 산학협력단 3D-mesh structure and impeller having 3D-mesh structure
KR101869350B1 (en) * 2015-06-26 2018-07-23 고려대학교 산학협력단 3D-mesh structure and impeller having 3D-mesh structure

Also Published As

Publication number Publication date
JPH04166221A (en) 1992-06-12

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