JPH04152827A - Grifola frondosa for high-speed culture of whole year - Google Patents
Grifola frondosa for high-speed culture of whole yearInfo
- Publication number
- JPH04152827A JPH04152827A JP2277512A JP27751290A JPH04152827A JP H04152827 A JPH04152827 A JP H04152827A JP 2277512 A JP2277512 A JP 2277512A JP 27751290 A JP27751290 A JP 27751290A JP H04152827 A JPH04152827 A JP H04152827A
- Authority
- JP
- Japan
- Prior art keywords
- strain
- maitake
- grifola frondosa
- culture
- mushrooms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000001080 Grifola frondosa Species 0.000 title claims abstract description 32
- 235000007710 Grifola frondosa Nutrition 0.000 title claims abstract description 30
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 241000233866 Fungi Species 0.000 abstract description 3
- 235000015099 wheat brans Nutrition 0.000 abstract description 3
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 229920001817 Agar Polymers 0.000 description 9
- 239000008272 agar Substances 0.000 description 9
- 239000002609 medium Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 239000006159 Sabouraud's agar Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000012225 czapek media Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000012449 sabouraud dextrose agar Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241001579016 Nanoa Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
［発明の目的１
（産業上の利用分野）
この発明は高速周年栽培用舞茸に関し、具体的には野生
の優良舞茸より抽出した優良菌株を数代にわたって純粋
培養育成した所望の舞茸から得られる舞茸の新菌株に係
り、更に詳細には、この新菌（株）によって栽培した舞
茸に関する。[Detailed Description of the Invention] [Objective of the Invention 1 (Field of Industrial Application) This invention relates to Maitake mushrooms for rapid year-round cultivation, and specifically, to pure culture of superior bacterial strains extracted from superior wild Maitake mushrooms over several generations. The present invention relates to a new strain of Maitake mushroom obtained from desired Maitake mushrooms that have been grown, and more specifically to Maitake mushrooms grown using this new strain.
（従来の技術）
従来の大ロー栽培用舞茸について略述すれば、次のとお
りである。(Prior Art) The conventional maitake mushrooms for cultivation in large rows are briefly described below.
程度のものが用いられる。なお、培養条件に対応してフ
スマの代替または添加物として、米糠、コーンプラン、
脱脂大豆粕、コーヒー抽出粕が適用される。A certain degree is used. Depending on the culture conditions, rice bran, corn plan,
Defatted soybean meal and coffee extract are applicable.
上記培養基２５〜２．８ｋｇをポリ袋に充填、殺菌後前
もって培養準備した種菌（市販の菌株）約１０ｇを接種
し、２２〜２５℃、暗所で培養する。A plastic bag is filled with 25 to 2.8 kg of the above culture medium, and after sterilization, about 10 g of a seed culture (commercially available strain) prepared in advance for culture is inoculated and cultured at 22 to 25° C. in a dark place.
次いで発生室に移し、光存在下で発芽、原基形成を行わ
せ、子実体の育成を行い、発生室移動後２０日間程度で
成熟舞茸として収穫される。The mushrooms are then transferred to a germination chamber, where they are allowed to germinate and form primordia in the presence of light, and fruiting bodies are grown.The mushrooms are harvested as mature Maitake mushrooms in about 20 days after being moved to the germination chamber.
（発明が解決しようとする課題）
この発明は、原料の菌株培養接子実体（キノコ）の収穫
までの日数を短縮する共に子実体の葉肉の厚い舞茸を得
るようにすると共にそのための新菌種を提供しようとす
るものである。(Problems to be Solved by the Invention) The present invention shortens the number of days until harvesting of the raw material strain-cultivated occidental bodies (mushrooms), makes it possible to obtain maitake mushrooms with thick mesophyll of the fruiting bodies, and develops a new fungus for this purpose. It is intended to provide seeds.
〔発明の構成］
（課題を解決するための手段）
この発明は、工業技術院微生物工業技術研究所に微工研
菌寄第 １１６５９号として寄託した舞茸菌（以下「舞
茸５Ｆ−７３菌」と云う）を用いて所望の舞茸を育成す
るようにしたものである。[Structure of the Invention] (Means for Solving the Problems) This invention is based on the Maitake fungus (hereinafter referred to as "Maitake 5F-73 fungus") deposited with the Institute of Microbial Technology, Agency of Industrial Science and Technology as FAIKEN Bacterial Submission No. 11659. '') to grow the desired mushrooms.
前記した舞茸５Ｆ−７３菌は；群馬県桐生市梅田町にあ
る通称「根本山」山中に自生していた舞茸より抽出した
野生の菌株を純粋培養して得たものである。The aforementioned Maitake mushroom 5F-73 bacterium was obtained by pure culture of a wild strain extracted from a Maitake mushroom that grows wild in the mountains commonly known as "Nemotoyama" in Umeda-cho, Kiryu City, Gunma Prefecture.
より具体的に説明すれば、前記野生株を培養して得られ
た優良株を多段階にわたってスクリーニングして得たも
のが、この発明に係る舞茸５Ｆ−７３菌である。More specifically, the Maitake 5F-73 strain according to the present invention is obtained by culturing the wild strain and screening superior strains obtained in multiple stages.
この舞茸５Ｆ−７３菌を培養して得られる舞茸は子実体
の葉肉が厚く食感が良好である。The Maitake mushroom obtained by culturing the Maitake 5F-73 bacteria has a thick mesophyll of the fruiting body and a good texture.
この発明に係る舞茸５Ｆ−７３菌の菌株を用いた場合の
舞茸と市販菌株より育生した舞茸との性状を比較して示
せば次のとおりである。The following is a comparison of the properties of Maitake mushrooms grown using the Maitake 5F-73 strain according to the present invention and Maitake mushrooms grown from commercially available strains.
培養基、５Ｆ−７３菌株についてはフスマ、市販菌株に
ついては前出のもとこれに米
糠、コーンプラン等を添加
培養温度；
５Ｆ−７３については至適温度２３℃
（菌糸伸長適温は２３〜２７℃）
（品温２４〜２５℃）
市販菌株については２２℃〜２５℃
市販菌株との市販
力ッコ内は９００ｍβポリビン使用の場合（作 用）
この発明に係る舞茸５Ｆ−７３菌の菌株は木質の分解が
速く菌糸の生長が極めて早い。従って、収穫までに要す
る期間を短くすることができる。その上培養基としては
、ふすま（麩）だけの単原料を使用するだけで良いので
市販菌株に使用している米糠、コーンプラン等の培養基
原料を減少させることができる。Culture medium, wheat bran for the 5F-73 strain, rice bran, corn plan, etc. for the commercially available strains as described above.Cultivation temperature: Optimum temperature for 5F-73 is 23℃ (optimum temperature for hyphal elongation is 23-27℃) ) (Product temperature: 24-25°C) 22°C-25°C for commercially available strains When using 900 mβ polyvinyl for commercially available strains (effect) The Maitake 5F-73 strain according to this invention is The wood decomposes quickly and the mycelium grows extremely quickly. Therefore, the period required until harvest can be shortened. Furthermore, since it is sufficient to use a single raw material such as wheat bran as a culture medium, it is possible to reduce the amount of culture medium raw materials such as rice bran and corn plan used in commercially available strains.
この発明の特性を培養番別に例示すれば次のとおりであ
る。The characteristics of this invention are illustrated by culture number as follows.
（１）麦芽エキス寒天培地を用いた場合麦芽エキス寒天
培地の内容
麦芽エキス ２０ｇ
ブドウ糖 ２０ｇ
ペプトン １ｇ
寒 天 ２５ｇ水
１ ０００ｍ ！※８日目目方ロニー径が
４４ｍｍ、中央部の径１５ｍｍ部分は菌糸が烹であるが
、周縁部はやや希薄。密な部分と希薄な部分との境界は
やや不明確。気菌糸はなく、白色。(1) When using malt extract agar medium Contents of malt extract agar medium Malt extract 20g Glucose 20g Peptone 1g Agar 25g Water
1000m! *On the 8th day, the diameter is 44mm, and the mycelium is hot in the 15mm diameter part of the center, but it is slightly thinner at the periphery. The boundary between dense and sparse areas is somewhat unclear. There are no aerial mycelium and the color is white.
（２）バレイ薯・ブドウ糖基天地（ＰＤＡ）を用いた場
合
ＰＤＡ寒天培地の内容
市販ＰＤＡ粉末 ３５ｇに水１℃
×８日目でコロニー径が４４ｍｍ、中央部がやや密で周
縁部に向かい徐々に希薄となる。気菌糸なし。(2) When using potato/glucose base (PDA) Contents of PDA agar medium 35 g of commercially available PDA powder and water at 1°C x Colony diameter was 44 mm on the 8th day, slightly dense in the center and gradually increasing towards the periphery. becomes diluted. No aerial mycelium.
（３）ツアペック寒天培地を用いた場合ツアペック培地
の内容
ＮａＮｏａ ３ ｇＫ２ＨＰ
０４ １ ｇＭｇ３０４７Ｈｉ
０ ０ 、 ５ ｇＫＣＩ
０．５ｇ
ＦｅＳＯ４７Ｈ２００，０１ｇ
シヨ糖 ３０ｇ
寒 天 １５ｇ
水 １． ０００ｍｊ２×８日目でご（
わずかな生育。１２日目方細い菌糸が極めて希薄に生育
。コロニーは不定形。気菌糸なし。白色。(3) When using Czapek agar medium Contents of Czapek medium NaNoa 3 gK2HP
04 1 gMg3047Hi
0 0, 5 gKCI
0.5g FeSO47H200.01g Cane sugar 30g Agar 15g Water 1. 000 mj 2 x 8th day (
slight growth. On the 12th day, thin mycelia grew extremely sparsely. Colonies are amorphous. No aerial mycelium. White.
（４）サブロー寒天培地を用いた場合
サブロー寒天培地の内容
マルトース ４０ｇ
ペプトン Ｌｏｇ
寒 天 １５ｇ
水 １ ０００ｍｊ２
×８日目で不定形の希薄なコロニー。１２日目方コロニ
ー径は４０ｍｍ、中央部から周縁部にかけて徐々に希薄
となる。コロニーの先端が不明瞭。気菌糸なし。白色。(4) When using Sabouraud agar medium Contents of Sabouraud agar medium Maltose 40 g Peptone Log agar 15 g Water 1 000 mj2 × Amorphous and sparse colonies on the 8th day. On the 12th day, the colony diameter was 40 mm and gradually became thinner from the center to the periphery. The tip of the colony is unclear. No aerial mycelium. White.
（５）ＹｐＳｓ寒天培地を用いた場合
ＹｐＳｓ寒天培地の内容
可溶性でんぷん １５ｇ
酵母エキス ４ｇ
Ｋ２ＨＰＯ４１ｇ
ＭｇＳ０４・７Ｈｚ０ ０ 、５ ｇ寒 天
２０ｇ
水 １． ０００ｍＩ２
×８日目で極めてわずかな生育。１２日１でコロニー径
が１６ｍｍ、中央部でやや密で周縁部にかけ徐々に希薄
となる。菌糸は細（、コロニー先端が不明瞭。気菌糸な
し。白色。(5) When using YpSs agar medium Contents of YpSs agar medium Soluble starch 15g Yeast extract 4g K2HPO41g MgS04.7Hz0 0, 5g agar
20g water 1. 000mI2 × Very little growth on the 8th day. On day 12, the colony diameter was 16 mm, slightly dense in the center and gradually becoming thinner towards the periphery. The hyphae are thin (the tip of the colony is unclear. There are no aerial hyphae. White.
（実施例）
この発明の実施例を表工および■として示したが、この
発明に係る舞茸５Ｆ−７３菌の菌株を用いて育生した舞
茸は市販菌株を用いて育生した舞茸に比較して表Ｉおよ
びＨに示すような優れた性状を有するものである。(Example) Examples of the present invention are shown as table work and ■, but Maitake mushrooms grown using the Maitake 5F-73 strain of this invention are compared to Maitake mushrooms grown using a commercially available strain. It has excellent properties as shown in Tables I and H.
［発明の効果］
上記したように、この発明に係る舞茸５Ｆ−７３菌の菌
株は；培養日数の点でサンバック方式で約１５％、ポリ
ビン方式で約１６．５％短縮でき、収穫量の声、で、サ
ンバック方式で約１４％、ポリビン方式で約２４％増収
にすることができるものであり、コストパホーマンスが
良い。[Effects of the Invention] As described above, the Maitake 5F-73 strain according to the present invention can reduce the number of cultivation days by approximately 15% using the Sunbag method and approximately 16.5% using the polyvin method, and has a high yield. The sunback method can increase sales by about 14%, and the polyvin method can increase sales by about 24%, so it has good cost performance.
また市販の舞茸に比較して特に子実体の葉肉が厚い点に
特徴を有するものである。Also, compared to commercially available Maitake mushrooms, it is characterized by the fact that the mesophyll of the fruiting body is particularly thick.
特許出願人 有限会社コンベックス 代理人 弁理士 大 塚 貞 次Patent applicant Convex Ltd. Agent: Patent Attorney Sadatsugu Otsuka
Claims (2)
の培養基で育生して舞茸を得るようにしたことを特徴と
する高速周年栽培用の菌株。(1) A strain for high-speed, year-round cultivation, characterized in that Maitake mushrooms are obtained by growing the Maitake fungus strain of Kaikoken Kyori No. 11659 in a desired culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2277512A JPH04152827A (en) | 1990-10-16 | 1990-10-16 | Grifola frondosa for high-speed culture of whole year |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2277512A JPH04152827A (en) | 1990-10-16 | 1990-10-16 | Grifola frondosa for high-speed culture of whole year |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04152827A true JPH04152827A (en) | 1992-05-26 |
Family
ID=17584634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2277512A Pending JPH04152827A (en) | 1990-10-16 | 1990-10-16 | Grifola frondosa for high-speed culture of whole year |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH04152827A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0648412A1 (en) * | 1993-09-20 | 1995-04-19 | Nihon Bayer Agrochem K.K. | Poisonous baits for controlling soil-inhabiting pests |
| CN105586269A (en) * | 2015-12-25 | 2016-05-18 | 江苏大学 | Method for producing grifola frondosa mycelium raw material from grifola frondosa strain obtained through mutagenesis |
| JP2019118278A (en) * | 2017-12-28 | 2019-07-22 | 株式会社ハイファ研究所 | Cultivation method of sparassia crispa and grifola frondosa using extract sludge of coffee and sparassia crispa in which functional component is enhanced |
-
1990
- 1990-10-16 JP JP2277512A patent/JPH04152827A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0648412A1 (en) * | 1993-09-20 | 1995-04-19 | Nihon Bayer Agrochem K.K. | Poisonous baits for controlling soil-inhabiting pests |
| US5882670A (en) * | 1993-09-20 | 1999-03-16 | Nihon Bayer Agrochem K.K. | Poisonous baits for controlling soil-inhabiting pests |
| CN105586269A (en) * | 2015-12-25 | 2016-05-18 | 江苏大学 | Method for producing grifola frondosa mycelium raw material from grifola frondosa strain obtained through mutagenesis |
| CN105586269B (en) * | 2015-12-25 | 2018-11-06 | 江苏大学 | The method for producing maitake mushroom mycelia raw material using mutagenesis Grifola frondosa strain |
| JP2019118278A (en) * | 2017-12-28 | 2019-07-22 | 株式会社ハイファ研究所 | Cultivation method of sparassia crispa and grifola frondosa using extract sludge of coffee and sparassia crispa in which functional component is enhanced |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101050856B1 (en) | Mushroom mycelium grains and their preparation method | |
| JP2007053928A (en) | New fungal strain and method for culturing new fruit body | |
| JP5124670B2 (en) | Mushroom artificial cultivation method | |
| JPH04152827A (en) | Grifola frondosa for high-speed culture of whole year | |
| KR100403411B1 (en) | The novel pleurotus ferulae-k9 and the culture method for thereof | |
| KR100395423B1 (en) | The novel pleurotus nebrodensis-k7 and the culture method for thereof | |
| JP3436768B2 (en) | Culture and cultivation method of new strain | |
| JP2004329129A (en) | Additive for culturing mushroom | |
| JP3542945B2 (en) | Artificial cultivation method of Hatake Shimeji | |
| JP2006067929A (en) | Method for culturing eryngii | |
| Nwufo et al. | Studies on the post-harvest rots of african breadfruit (Treculia africana) seeds in Nigeria | |
| Bhosale et al. | Influence of different growing media on mass production of Aschersonia aleyrodis, a fungal pathogen of citrus black fly | |
| JP3482410B2 (en) | A new basidiomycete, Bunakaritake strain | |
| JP4397047B2 (en) | A new strain of Honshimeji | |
| JP4202541B2 (en) | Artificial cultivation method of Honshimeji | |
| JPH06311827A (en) | New strain of lyophyllum decastes and culture method | |
| JP3229287B2 (en) | Mushroom artificial cultivation method | |
| JP4685821B2 (en) | Artificial cultivation method of Honshimeji | |
| JP4751409B2 (en) | Artificial cultivation method of Honshimeji | |
| JP3539953B2 (en) | New strain of lyophilum ulmarium | |
| JP4132536B2 (en) | Artificial cultivation method of Honshimeji | |
| JP3827456B2 (en) | New strain and production method | |
| JP2000300066A (en) | Culture of mycoleptodonoides aitchisonii | |
| US20200100415P1 (en) | Maitake mushroom named 'Grifon-8go' | |
| JP2006271234A (en) | New strain of lyophyllum shimeji and use of the same |