JPH0413655B2 - - Google Patents
Info
- Publication number
- JPH0413655B2 JPH0413655B2 JP59085533A JP8553384A JPH0413655B2 JP H0413655 B2 JPH0413655 B2 JP H0413655B2 JP 59085533 A JP59085533 A JP 59085533A JP 8553384 A JP8553384 A JP 8553384A JP H0413655 B2 JPH0413655 B2 JP H0413655B2
- Authority
- JP
- Japan
- Prior art keywords
- methane
- wavelength
- fluorescence
- bacteria
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 172
- 241000894006 Bacteria Species 0.000 claims description 61
- 230000005284 excitation Effects 0.000 claims description 30
- 230000000694 effects Effects 0.000 claims description 22
- 238000012360 testing method Methods 0.000 claims description 7
- 230000001678 irradiating effect Effects 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 3
- 239000012491 analyte Substances 0.000 claims 1
- 230000001747 exhibiting effect Effects 0.000 claims 1
- 239000000126 substance Substances 0.000 description 25
- 230000004151 fermentation Effects 0.000 description 18
- 238000000855 fermentation Methods 0.000 description 18
- 244000005700 microbiome Species 0.000 description 17
- 238000000034 method Methods 0.000 description 15
- 238000002189 fluorescence spectrum Methods 0.000 description 14
- 238000005259 measurement Methods 0.000 description 12
- 238000012937 correction Methods 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 11
- 238000010586 diagram Methods 0.000 description 10
- 238000001228 spectrum Methods 0.000 description 10
- 238000000695 excitation spectrum Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 238000001514 detection method Methods 0.000 description 6
- 238000000691 measurement method Methods 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- 239000005515 coenzyme Substances 0.000 description 5
- 239000010802 sludge Substances 0.000 description 5
- 230000003595 spectral effect Effects 0.000 description 5
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 4
- 241001074903 Methanobacteria Species 0.000 description 4
- 230000000696 methanogenic effect Effects 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000037149 energy metabolism Effects 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 239000010865 sewage Substances 0.000 description 3
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 2
- 230000002238 attenuated effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 229950006238 nadide Drugs 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 1
- 108091006149 Electron carriers Proteins 0.000 description 1
- 241000205275 Methanosarcina barkeri Species 0.000 description 1
- XJLXINKUBYWONI-NNYOXOHSSA-N NADP zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000027721 electron transport chain Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000013214 routine measurement Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59085533A JPS60230040A (ja) | 1984-04-27 | 1984-04-27 | メタン菌の菌数またはメタン生成活性の測定方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59085533A JPS60230040A (ja) | 1984-04-27 | 1984-04-27 | メタン菌の菌数またはメタン生成活性の測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60230040A JPS60230040A (ja) | 1985-11-15 |
| JPH0413655B2 true JPH0413655B2 (fr) | 1992-03-10 |
Family
ID=13861520
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59085533A Granted JPS60230040A (ja) | 1984-04-27 | 1984-04-27 | メタン菌の菌数またはメタン生成活性の測定方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60230040A (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0762652B2 (ja) * | 1986-08-22 | 1995-07-05 | 株式会社日立製作所 | けい光分析方法およびその装置 |
| JPS63247643A (ja) * | 1987-04-02 | 1988-10-14 | Kondo Kogyo Kk | 浮遊細菌の計測方法 |
| JP5148387B2 (ja) * | 2008-06-30 | 2013-02-20 | 浜松ホトニクス株式会社 | 分光測定装置、分光測定方法、及び分光測定プログラム |
| US20130078730A1 (en) * | 2011-09-23 | 2013-03-28 | Michael J. Murcia | Method for monitoring and control of a wastewater process stream |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5194886A (fr) * | 1975-02-19 | 1976-08-19 | ||
| JPS5543461A (en) * | 1978-09-22 | 1980-03-27 | Gunze Ltd | Corrected turbidity measuring method for treating agent concentration containing in suspension |
-
1984
- 1984-04-27 JP JP59085533A patent/JPS60230040A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5194886A (fr) * | 1975-02-19 | 1976-08-19 | ||
| JPS5543461A (en) * | 1978-09-22 | 1980-03-27 | Gunze Ltd | Corrected turbidity measuring method for treating agent concentration containing in suspension |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60230040A (ja) | 1985-11-15 |
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