JPH04117278A - Method for controlling plant disease damage using symbiotic microorganism and new microorganism used in practice of the method - Google Patents

Method for controlling plant disease damage using symbiotic microorganism and new microorganism used in practice of the method

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Publication number
JPH04117278A
JPH04117278A JP2236392A JP23639290A JPH04117278A JP H04117278 A JPH04117278 A JP H04117278A JP 2236392 A JP2236392 A JP 2236392A JP 23639290 A JP23639290 A JP 23639290A JP H04117278 A JPH04117278 A JP H04117278A
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Japan
Prior art keywords
microorganism
microorganism according
novel
disease
cultured
Prior art date
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Granted
Application number
JP2236392A
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Japanese (ja)
Other versions
JPH07108848B2 (en
Inventor
Toshio Kijima
木嶋 利男
Sadao Yoneuchi
米内 貞夫
Kazuo Ohashi
一夫 大橋
Masayuki Amaya
天谷 正行
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TOCHIGI PREF GOV
Tochigi Prefecture
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TOCHIGI PREF GOV
Tochigi Prefecture
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Priority to JP2236392A priority Critical patent/JPH07108848B2/en
Publication of JPH04117278A publication Critical patent/JPH04117278A/en
Publication of JPH07108848B2 publication Critical patent/JPH07108848B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

PURPOSE:To control a pathogenic fungus by antagonism by proliferation of a microorganism and provide resistance to disease damage to a germ-free culture seedling by separating the microorganism symbiosed in a disease-free tissue of a plant and inoculating a seedling having affinity with the microorganism with the microorganism. CONSTITUTION:A microorganism symbiosed in a disease-free tissue of a plant is separated and the separated and cultured microorganism is inoculated into an ordinary culture seedling or germ-free culture seedling capable of exhibiting affinity with the microorganism. As a result, a pathogenic fungus is controlled by antagonism by proliferation of the microorganism. The above-mentioned microorganism includes e.g. Pseudomonas CP-098 (FERM-11629) providing resistance to disease of cyclamen.

Description

【発明の詳細な説明】 [発明の目的コ (産業上の利用分野) 本発明は、共生微生物の拮抗作用を利用した植物病害の
防除方法およびその方法の実施に用いる新規微生物に関
する。
DETAILED DESCRIPTION OF THE INVENTION [Purpose of the Invention (Field of Industrial Application) The present invention relates to a method for controlling plant diseases that utilizes the antagonistic action of symbiotic microorganisms, and a novel microorganism used to carry out the method.

(従来技術) 本発明者らの知る限りにおいて、共生微生物を分離培養
し、これを親和性を示す苗に接種して、その増殖による
拮抗作用によって病原菌の駆除を可能とする植物病害の
防除技術は、これまでに提案、実施されていない。
(Prior art) To the best of the knowledge of the present inventors, a plant disease control technology that enables the extermination of pathogenic bacteria by the antagonistic action of the multiplication of symbiotic microorganisms by isolating and culturing them and inoculating them into seedlings that exhibit affinity for them. has not been proposed or implemented to date.

(発明が解決しようとする課題) 本発明者らは、シクラメンとサツマイモの無菌培養の研
究を進める段階で、これら植物の無病徴組織内に細菌や
糸状菌が共生していること、その共生部位が根、塊茎、
葉柄、葉身、花便、がくてあり、種子には認められない
こと、その種子を無菌培養することでこれら植物の無菌
培養が可能であることく実験に成功)、そしてこのよう
にして無菌培養された植物の苗を自然条件下にさらすと
、またもとの細菌及び糸状菌の共生状態に戻ることくシ
クラメン及びサツマイモは常に組織内に共生微生物を持
っている)、などを知見した。
(Problems to be Solved by the Invention) The present inventors discovered that bacteria and filamentous fungi coexist within the disease-free tissues of these plants, and that the symbiotic sites are roots, tubers,
(The experiment was successful in that it was possible to cultivate these plants aseptically by culturing the seeds aseptically.) They found that when cultured plant seedlings are exposed to natural conditions, they return to the original symbiotic state of bacteria and filamentous fungi (cyclamen and sweet potatoes always have symbiotic microorganisms in their tissues).

ところで無菌培養苗は、単一個体を増殖するため、遺伝
的形質も同一なものとなる。この同一形質は組織培養で
は必要なことであるが、デメリットとして、病害に対し
ても同一抵抗性反応を示すため、−旦病害の発生に遭遇
すると、前記のように遺伝形質が同一であるため、場合
によっては全滅することにもなる。
By the way, since sterile cultured seedlings propagate as a single individual, their genetic traits are also the same. This same trait is necessary in tissue culture, but the disadvantage is that it shows the same resistance response to diseases, and once a disease outbreak occurs, the genetic traits are the same as mentioned above. , and in some cases even become extinct.

そこで、このような無菌培養苗には病害の抵抗性を付与
することが不可欠の課題となる。
Therefore, it is essential to impart disease resistance to such sterile cultured seedlings.

本発明者らは上記実情からさらに研究を進め、上記のよ
うにシクラメン及びサツマイモが常に組織内に共生微生
物を持っていることに着目し、それらの無病徴組織内に
共生する有用微生物を分離し、これを無菌培養苗に接種
して抵抗性を付与することを着想、実現し得たものであ
る。
The present inventors conducted further research based on the above circumstances, focused on the fact that cyclamen and sweet potatoes always have symbiotic microorganisms in their tissues, and isolated useful microorganisms that coexist in their non-symptomatic tissues. They came up with the idea of inoculating this into sterile cultured seedlings to impart resistance.

又これに関与する有用微生物が、いずれも新規な微生物
であることが明らかとなったものである。
It has also been revealed that the useful microorganisms involved in this are all new microorganisms.

[課題を解決するための手段] く防除方法にいて) すなわち本発明は、植物の無病徴組織内に共生する微生
物(以下「共生微生物」という)を各別に分離し、その
分離培養した微生物の単数あるいは複数を、これらと親
和性を示す一般栽培苗または無菌培養苗に接種して、そ
の増殖による拮抗作用によって病原菌の駆除を可能とす
る植物病害の防除方法である。
[Means for Solving the Problems] In other words, the present invention involves separating each microorganism that coexists in the disease-free tissue of a plant (hereinafter referred to as "symbiotic microorganism"), and culturing the isolated and cultured microorganism. This is a method for controlling plant diseases, in which one or more of these pathogens are inoculated into commonly cultivated seedlings or aseptically cultured seedlings that have an affinity for these, and the antagonistic effect of their multiplication enables the extermination of pathogenic bacteria.

そして、その植物がシクラメンであり、その共生微生物
から分離した有用微生物をシクラメンの苗に接種するこ
とにより、炭そ病を防除する方法である。
The plant is a cyclamen, and the method is to control anthracnose by inoculating cyclamen seedlings with useful microorganisms separated from the symbiotic microorganisms.

また植物がサツマイモであり、その共生微生物から分離
した有用微生物をサツマイモの苗に接種することにより
、つる割病を防除する方法である。
In addition, the plant is a sweet potato, and this is a method for controlling vine wart by inoculating sweet potato seedlings with useful microorganisms separated from the symbiotic microorganisms.

(共生微生物について) さらに、その無病徴絹織内に共生し、これより分離した
微生物、エルヴイニア(Erwinia)、シュードモ
ナス(Pseudomonas)。
(Regarding symbiotic microorganisms) Furthermore, microorganisms such as Erwinia and Pseudomonas coexist within the disease-free silk fabric and are isolated from it.

バシラス(Bac i l 1us)+キサントモナス
(Xanthomonas>に属する菌を新規な微生物
として、併せて特許請求するものである。
Bacteria belonging to Bacillus + Xanthomonas are claimed as novel microorganisms.

植物の各部位の無病徴紹織内から分離される細@(共生
微生物)を、シクラメン(実生系、クローン系)とサツ
マイモについて見ると、下表の通りである。
The table below shows the symbiotic microorganisms isolated from the disease-free tissue of each part of the plant for cyclamen (seedling type, clone type) and sweet potato.

く第1表〉 シクラメンの各部位から分離される細菌 部位        細  菌 実生系     クローン系 塊茎 1.lXl0   4.9X10芽    1.
 3X104   2. 8X104葉柄 4.6X1
0   1.5X104葉身 2.lXl0   3.
3X10”λ 葉梗 3.0XIO2,0xlO” 花弁    OO 釣     OO 〈第2表〉 シクラメンの各部位から分離される細菌の
種類 部位        細  菌 種子 E r w i n i a 、未同定塊茎 E
rwinia、Pseudomonas芽    Er
win  ia、  Pseudomonas葉柄 E
rwinia、Bac i I lus葉身 Erwi
nia、Bac i l Ius葉梗 Erwinia
、Bac i I Iusく第3表〉 サツマイモ(1
乃至5)から分離される細菌 細  菌     分離場所 葉−11,8X10    栃木県藤岡町葉柄−11,
3X10    栃木県藤岡町茎−21,2X104 
  栃木県藤岡町葉柄−27,9X10    栃本県
膝岡町茎−32,lX10    栃木県藤岡町葉柄−
34,7X10    栃木県藤岡町ジ 茎−41,lXl0    栃木県藤岡町一 葉柄−42,9X10    栃木県藤岡町? 茎−52,lXl0    栃木県岩舟町ユ 葉柄−54,0X10    栃本県岩舟町く第4表〉
 サツマイモの各部位から分離される細菌の種類 部位        細  菌 茎  Pseudomonas、  Bac  i  
l  IusXanthomonas、未同定 葉柄      〃 上記のとおり分離された微生物は、シクラメンでは、P
seudomonas、Erwinia。
Table 1 Bacterial parts isolated from each part of cyclamen Bacterial seedling system Clonal tuber system 1. lXl0 4.9X10 buds 1.
3X104 2. 8X104 petiole 4.6X1
0 1.5X104 leaf blade 2. lXl0 3.

rwinia, Pseudomonas bud Er
win ia, Pseudomonas petiole E
rwinia, Bac i I lus leaf blade Erwi
nia, Bac i l Ius Erwinia
, Bac i I Ius Table 3〉 Sweet potato (1
Bacteria isolated from 5) Isolation location Leaf-11, 8X10 Leaf stalk-11, Fujioka-cho, Tochigi Prefecture
3X10 Tochigi Prefecture Fujioka-cho Kuki-21, 2X104
Petiole, Fujioka Town, Tochigi Prefecture - 27,9X10 Stem, Kneeoka Town, Tochimoto Prefecture - 32, lX10 Petiole, Fujioka Town, Tochigi Prefecture -
34.7 Stem - 52, lXl0 Tochigi Prefecture Iwafune Town Yu Petiole - 54,0X10 Tochimoto Prefecture Iwafune Town Table 4>
Types of bacteria isolated from each part of sweet potato Part Bacterial stem Pseudomonas, Bac i
l IusXanthomonas, unidentified petiole The microorganism isolated as above is P
seudomonas, Erwinia.

Bacillusに属するものであり、シクラメンに接
種することで抵抗性が付与されるものとして、Pseu
domonasではCP−098(微工研菌寄第116
29号)、ErwiniaではCE−057(微工研菌
寄第11630号)、BacillusではCB−13
1(tfi工研菌寄第11625号)が選抜され、いず
れも別記の細菌学的性質を有することから、新規微生物
であることが明らかとなフた。またサツマイモでは、P
seudomonas、Xanthomonas。
Pseu belongs to Bacillus and can be given resistance by inoculating cyclamen.
domonas has CP-098
29), CE-057 for Erwinia (Feikoken Bacteria No. 11630), and CB-13 for Bacillus.
1 (TFI Koken Bacteria No. 11625) was selected, and since both had the bacteriological properties specified separately, it was clear that they were novel microorganisms. Also, in sweet potatoes, P
seudomonas, Xanthomonas.

Bacillusに属するものであり、サツマイモに接
種することで抵抗性が付与されるものとして、Pseu
domonasではBP−084(微工研菌寄第116
27号)、XanthomonasではBX−146(
tM工研菌寄第11628号)、Bacillusては
BB−145(微工研菌寄第11626号)が選抜され
、いずれも別記の細菌学的性質を有することから、新規
微生物であることが明らかとなった。
Pseu belongs to Bacillus and can be given resistance by inoculating sweet potatoes.
Domonas has BP-084 (Feikoken Bacterium 116
27), Xanthomonas has BX-146 (
tM Koken Bacillus No. 11628) and Bacillus BB-145 (Feikoken Bacilli No. 11626) were selected, and both have the bacteriological properties specified separately, so it is clear that they are new microorganisms. It became.

細菌学的性質(CP 098〉 m菌学的性質(CB 細菌学的性質(CE 057〉 細菌学的性@ (BP O84) 細菌学的性質(BX−146) 細菌学的性質(BB−1451 [発明の作用効果] これら分離された微生物を用い、本発明を実施した結果
、病害防除の効果を遺憾なく発揮した。
Bacteriological properties (CP 098> m Mycological properties (CB Bacteriological properties (CE 057) Bacteriological properties @ (BP O84) Bacteriological properties (BX-146) Bacteriological properties (BB-1451 [ Effects of the Invention] As a result of carrying out the present invention using these isolated microorganisms, the effect of disease control was fully demonstrated.

即ちこれらの大部分の菌株は、一般栽培および焦面組織
培養のシクラメン、サツマイモに親和性を示し、病原性
を示さなかった。シクラメンにおいては、これらを接種
すると、炭そ病に対して抵抗性を示し、その後接種した
炭そ病の発病を抑えた。またサツマイモにおいては、こ
れらを接種すると、つる割病に対して抵抗性を示し、そ
の後接種したつる割病の発病を抑えた。
That is, most of these strains showed affinity for cyclamen and sweet potato grown in conventional cultivation and focal tissue culture, and did not exhibit pathogenicity. Cyclamen showed resistance to anthracnose when inoculated with these, and suppressed the onset of anthracnose that was subsequently inoculated. In addition, when sweet potatoes were inoculated with these, they showed resistance to vine wart and suppressed the onset of vine wart that was subsequently inoculated.

その防除試験結果を示すと下表のとおりである。The results of the control tests are shown in the table below.

く炭そ病についての防除試験結果〉 処理菌株 無菌苗にお 再分離  病原菌接種ける病原
性      による発病 接種 CB−131 CP−098 CE−057 無接種 + + + + くつる割病についての防除試験結果〉 処理菌株 無菌苗にお 再分離  病原菌接種ける病原
性      による発病 接種 BX−146 BP−084 8B−145 無接種 十 + + + 本発明は以上のようで、無菌培養菌に病害の抵抗性を付
与するという不可欠の課題を、共生微生物の分離、接種
という生物的方法で解消する点で、まことに意義が大き
いものである。
Results of control test for anthracnose Treated strain Re-separation into sterile seedlings Pathogenicity caused by inoculation of pathogenic bacteria Inoculation CB-131 CP-098 CE-057 No inoculation + + + + Control test results for anthracnose 〉 Treated bacterial strain Re-separated into sterile seedlings Inoculated with pathogenic bacteria BX-146 BP-084 8B-145 Non-inoculated 10+ + + The present invention is as described above and imparts disease resistance to sterile cultured bacteria. This is of great significance in that it solves the essential problem of using the biological method of isolating and inoculating symbiotic microorganisms.

Claims (1)

【特許請求の範囲】 1)植物の無病徴組織内に共生する微生物を各別に分離
し、その分離培養した微生物の単数あるいは複数を、こ
れらと親和性を示す一般栽培苗または無菌培養苗に接種
して、その増殖による拮抗作用によって病原菌の駆除を
可能とした植物病害の防除方法。 2)植物がシクラメンであり、その無病徴組織内に共生
する微生物が、エルヴィニア(Erwinia)、シュ
ードモナス(Pseudomonas)、バシラス(B
acillus)に属する特許請求の範囲第1項記載の
共生微生物を用いた植物病害の防除方法。 3)植物がサツマイモであり、その無病徴組織内に共生
する微生物が、シュードモナス(Pseudomona
s)、バシラス(Bacillus)、キサントモナス
(Xanthomonas)に属する特許請求の範囲第
1項記載の共生微生物を用いた植物病害の防除方法。 4)分離培養した微生物の接種対象植物が、シクラメン
である特許請求の範囲第2項記載の共生微生物を用いた
植物病害の防除方法。 5)分離培養した微生物の接種対象植物が、サツマイモ
である特許請求の範囲第3項記載の共生微生物を用いた
植物病害の防除方法。 6)シクラメンの無病徴組織内に共生し、これより各別
に分離培養でき、シクラメンの一般栽培苗または無菌培
養苗に親和性を有し且つ非病原性である新規微生物。 7)エルヴィニア(Erwinia)属に属する特許請
求の範囲第6項記載の新規微生物。 8)エルヴィニア(Erwinia)CE−057(微
工研菌寄第11630号)株である特許請求の範囲第7
項記載の新規微生物。 9)シュードモナス(Pseudomonas)属に属
する特許請求の範囲第6項記載の新規微生物。 10)シュードモナス(Pseudomonas)CP
−098(微工研菌寄第11629号)株である特許請
求の範囲第9項記載の新規微生物。 11)バシラス(Bacillus)属に属する特許請
求の範囲第6項記載の新規微生物。 12)バシラス(Bacillus)CB−131(微
工研菌寄第11625号)株である特許請求の範囲第1
1項記載の新規微生物。 13)サツマイモの無病徴組織内に共生し、これより各
別に分離培養でき、サツマイモの一般栽培苗または無菌
培養苗に親和性を有し且つ非病原性である新規微生物。 14)キサントモナス(Xanthomonas)属に
属する特許請求の範囲第13項記載の新規微生物。 15)キサントモナス(Xanthomonas)BX
−146(微工研菌寄第11628号)株である特許請
求の範囲第14項記載の新規微生物。 16)シュードモナス(Pseudomonas)属に
属する特許請求の範囲第13項記載の新規微生物。 17)シュードモナス(Pseudomonas)BP
−084(微工研菌寄第11627号)株である特許請
求の範囲第16項記載の新規微生物。 18)バシラス(Bacillus)属に属する特許請
求の範囲第13項記載の新規微生物。 19)バシラス(Bacillus)BB−145(微
工研菌寄第11626号)株である特許請求の範囲18
項記載の新規微生物。
[Scope of Claims] 1) Separating each microorganism that coexists in the disease-free tissue of a plant, and inoculating one or more of the isolated and cultured microorganisms into commonly cultivated seedlings or sterile cultured seedlings that have an affinity for these microorganisms. A method for controlling plant diseases that makes it possible to exterminate pathogenic bacteria through the antagonistic effect of their multiplication. 2) The plant is a cyclamen, and the microorganisms that coexist within its disease-free tissue include Erwinia, Pseudomonas, and Bacillus.
A method for controlling plant diseases using the symbiotic microorganism according to claim 1 belonging to P. acillus. 3) The plant is a sweet potato, and the microorganism that coexists within its disease-free tissue is Pseudomonas.
2. A method for controlling plant diseases using the symbiotic microorganism according to claim 1, which belongs to the genus Bacillus s), Bacillus, and Xanthomonas. 4) A method for controlling plant diseases using a symbiotic microorganism according to claim 2, wherein the plant to be inoculated with the isolated and cultured microorganism is a cyclamen. 5) A method for controlling plant diseases using a symbiotic microorganism according to claim 3, wherein the plant to be inoculated with the isolated and cultured microorganism is a sweet potato. 6) A novel microorganism that coexists in disease-free tissues of cyclamen, can be isolated and cultured separately from these, has an affinity for commonly cultivated cyclamen seedlings or aseptically cultured seedlings, and is nonpathogenic. 7) The novel microorganism according to claim 6, which belongs to the genus Erwinia. 8) Claim 7, which is Erwinia CE-057 (Feikoken Bacteria No. 11630) strain
Novel microorganisms described in Section. 9) The novel microorganism according to claim 6, which belongs to the genus Pseudomonas. 10) Pseudomonas CP
11. The novel microorganism according to claim 9, which is strain -098 (Feikoken Bacteria No. 11629). 11) The novel microorganism according to claim 6, which belongs to the genus Bacillus. 12) Claim 1, which is Bacillus CB-131 (Feikoken Bacterial Serial No. 11625) strain
The novel microorganism described in item 1. 13) A novel microorganism that coexists in disease-free tissues of sweet potatoes, can be isolated and cultured separately from these, has an affinity for commonly cultivated sweet potato seedlings or aseptically cultured seedlings, and is nonpathogenic. 14) The novel microorganism according to claim 13, which belongs to the genus Xanthomonas. 15) Xanthomonas BX
15. The novel microorganism according to claim 14, which is strain -146 (Feikoken Bacterium No. 11628). 16) The novel microorganism according to claim 13, which belongs to the genus Pseudomonas. 17) Pseudomonas BP
17. The novel microorganism according to claim 16, which is the strain -084 (Feikoken Bacteria No. 11627). 18) The novel microorganism according to claim 13, which belongs to the genus Bacillus. 19) Claim 18 which is Bacillus BB-145 (Feikoken Bacterial Serial No. 11626) strain
Novel microorganisms described in Section.
JP2236392A 1990-09-06 1990-09-06 Method for controlling plant diseases using symbiotic microorganisms and novel microorganisms used for implementing the method Expired - Lifetime JPH07108848B2 (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935571A (en) * 1996-05-20 1999-08-10 Taki Chemical Co., Ltd. Plug mixture for raising seedlings and method for producing it, and method for raising disease tolerant seedlings
WO2008032693A1 (en) 2006-09-15 2008-03-20 Idemitsu Kosan Co., Ltd. Material for control of soil-borne disease in plant utilizing novel filamentous bacterium
JP2017002025A (en) * 2015-06-12 2017-01-05 株式会社前川製作所 Novel agricultural use for pseudomonas bacteria
WO2021012001A1 (en) * 2019-07-19 2021-01-28 Agriculture Victoria Services Pty Ltd Novel xanthomonas strains and related methods

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101038382B1 (en) * 2008-07-14 2011-06-01 안동대학교 산학협력단 An Entomopathogenic Bacterium, Serratia sp. ANU101 Isolated from an Entomopathogenic Nematode, Steinernema monticolum

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JPS63190806A (en) * 1987-02-03 1988-08-08 Sumitomo Chem Co Ltd Controlling of soil bright of vegetables by plant root plant bacteria
JPS63291578A (en) * 1986-08-09 1988-11-29 Tochigi Pref Gov Novel microorganism and control of fusarium wilt or such using said microorganism
JPH03101606A (en) * 1989-09-14 1991-04-26 Central Glass Co Ltd Control method for soft rot

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63291578A (en) * 1986-08-09 1988-11-29 Tochigi Pref Gov Novel microorganism and control of fusarium wilt or such using said microorganism
JPS63190806A (en) * 1987-02-03 1988-08-08 Sumitomo Chem Co Ltd Controlling of soil bright of vegetables by plant root plant bacteria
JPH03101606A (en) * 1989-09-14 1991-04-26 Central Glass Co Ltd Control method for soft rot

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935571A (en) * 1996-05-20 1999-08-10 Taki Chemical Co., Ltd. Plug mixture for raising seedlings and method for producing it, and method for raising disease tolerant seedlings
WO2008032693A1 (en) 2006-09-15 2008-03-20 Idemitsu Kosan Co., Ltd. Material for control of soil-borne disease in plant utilizing novel filamentous bacterium
JP2017002025A (en) * 2015-06-12 2017-01-05 株式会社前川製作所 Novel agricultural use for pseudomonas bacteria
WO2021012001A1 (en) * 2019-07-19 2021-01-28 Agriculture Victoria Services Pty Ltd Novel xanthomonas strains and related methods

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