JPH04368306A - New fungus, fungus-containing herbicide and controlling weed thereby - Google Patents
New fungus, fungus-containing herbicide and controlling weed therebyInfo
- Publication number
- JPH04368306A JPH04368306A JP3242900A JP24290091A JPH04368306A JP H04368306 A JPH04368306 A JP H04368306A JP 3242900 A JP3242900 A JP 3242900A JP 24290091 A JP24290091 A JP 24290091A JP H04368306 A JPH04368306 A JP H04368306A
- Authority
- JP
- Japan
- Prior art keywords
- fungus
- water chestnut
- pathogenicity
- microorganism
- new
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000196324 Embryophyta Species 0.000 title claims abstract description 21
- 230000002363 herbicidal effect Effects 0.000 title claims abstract description 10
- 239000004009 herbicide Substances 0.000 title claims abstract description 9
- 241000233866 Fungi Species 0.000 title abstract 6
- 238000000034 method Methods 0.000 claims abstract description 11
- 244000005700 microbiome Species 0.000 claims description 35
- 230000007918 pathogenicity Effects 0.000 abstract description 10
- 235000007688 Lycopersicon esculentum Nutrition 0.000 abstract description 8
- 240000003768 Solanum lycopersicum Species 0.000 abstract description 8
- 244000061458 Solanum melongena Species 0.000 abstract description 8
- 235000002597 Solanum melongena Nutrition 0.000 abstract description 8
- 235000021307 Triticum Nutrition 0.000 abstract description 6
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 244000046052 Phaseolus vulgaris Species 0.000 abstract description 5
- 208000024891 symptom Diseases 0.000 abstract description 5
- 241000013911 Dendryphiella Species 0.000 abstract description 4
- 238000011534 incubation Methods 0.000 abstract description 3
- 238000012216 screening Methods 0.000 abstract description 2
- 235000003283 Pachira macrocarpa Nutrition 0.000 abstract 6
- 241001083492 Trapa Species 0.000 abstract 6
- 235000014364 Trapa natans Nutrition 0.000 abstract 6
- 235000009165 saligot Nutrition 0.000 abstract 6
- 240000007594 Oryza sativa Species 0.000 abstract 3
- 230000001276 controlling effect Effects 0.000 abstract 2
- 235000002848 Cyperus flabelliformis Nutrition 0.000 abstract 1
- 235000014968 Peltiphyllum peltatum Nutrition 0.000 abstract 1
- 244000104677 Peltiphyllum peltatum Species 0.000 abstract 1
- 244000098338 Triticum aestivum Species 0.000 abstract 1
- 235000005324 Typha latifolia Nutrition 0.000 abstract 1
- 244000118869 coast club rush Species 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 241000576755 Sclerotia Species 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 241001638133 Bidyanus welchi Species 0.000 description 10
- 241000209094 Oryza Species 0.000 description 9
- 235000007164 Oryza sativa Nutrition 0.000 description 9
- 235000009566 rice Nutrition 0.000 description 9
- 239000001965 potato dextrose agar Substances 0.000 description 7
- 241000218691 Cupressaceae Species 0.000 description 5
- 241000209140 Triticum Species 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 235000021332 kidney beans Nutrition 0.000 description 4
- 241000254060 Aquatica lateralis Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 244000013123 dwarf bean Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000021331 green beans Nutrition 0.000 description 3
- 241001519451 Abramis brama Species 0.000 description 2
- 244000058871 Echinochloa crus-galli Species 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- FYLGZBNNVGZPIW-UHFFFAOYSA-N 1-[4-(1,4-dioxa-8-azaspiro[4.5]decan-8-yl)phenyl]ethanone Chemical compound C1=CC(C(=O)C)=CC=C1N1CCC2(OCCO2)CC1 FYLGZBNNVGZPIW-UHFFFAOYSA-N 0.000 description 1
- 241001247278 Acanthopagrus schlegelii Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- QXJJQWWVWRCVQT-UHFFFAOYSA-K calcium;sodium;phosphate Chemical compound [Na+].[Ca+2].[O-]P([O-])([O-])=O QXJJQWWVWRCVQT-UHFFFAOYSA-K 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【０００１】0001
【産業上の利用分野】本発明は、新規微生物、デンドリ
フィエラ属に属する微生物を含有する除草剤およびそれ
らによる雑草の防除方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a new microorganism, a herbicide containing a microorganism belonging to the genus Dendriphiella, and a method for controlling weeds using the same.
【０００２】0002
【従来技術】水田の雑草を微生物を用いて防除する方法
、とくに、多年生の難防除雑草として知られるクログワ
イに対する防除方法として、ハイホミセテスの新属菌を
用いる防除方法に関する発明（特開平１−２３８５０７
号公報）が知られている。[Prior Art] An invention relating to a method of controlling weeds in rice fields using microorganisms, in particular, a method of controlling weeds using a new genus of Hyhomycetes (Japanese Unexamined Patent Publication No. 1-238507)
Publication No.) is known.
【０００３】0003
【発明が解決しようとする課題】上記従来技術にあって
は、ハイホミセテス新属菌の病徴発現までの潜伏期間が
約２週間と長く、速効性の点で解決すべき課題をもって
いた。本発明は、従来技術にみられる上記課題を解決し
ようとするものであり、デンドリフィエラ新属菌（Ｄｅ
ｎｄｒｙｐｈｉｅｌｌａ属）の新規微生物を提供するこ
とを目的とする。[Problems to be Solved by the Invention] In the above-mentioned prior art, the incubation period for the new genus Hyhomycetes until disease symptoms appear is as long as about two weeks, and there is a problem to be solved in terms of rapid efficacy. The present invention aims to solve the above-mentioned problems seen in the prior art, and aims to solve the problems described above in the prior art.
The purpose of this invention is to provide a new microorganism of the genus ndryphiella.
【０００４】また、本発明は、デンドリフィエラ属に属
する微生物を含有することを特徴とする除草剤を提供す
ることを目的とする。さらに、本発明は、それらを用い
ることを特徴とする雑草の防除方法を提供することを目
的とするものである。Another object of the present invention is to provide a herbicide characterized by containing a microorganism belonging to the genus Dendriphiella. A further object of the present invention is to provide a method for controlling weeds characterized by using these methods.
【０００５】[0005]
【課題を解決するための手段】本発明の新規微生物は、
デンドリフィエラ新属菌（Ｄｅｎｄｒｙｐｈｉｅｌ−ｌ
ａ属）Ｋ−０４１（微工研菌寄第１２２８０号）である
。上記新規微生物は、日本各地より罹病したクログワイ
を収集し、クログワイに対し病原性を有する菌株を分離
して、純粋培養し、クログワイに対する防除効果を検討
するとともに、他の作物、特に、イネに対する非感染性
を検討するスクリーニング手段によって創製したもので
ある。[Means for solving the problems] The novel microorganism of the present invention is
Dendryphiel-l
a genus) K-041 (Feikoken Bibori No. 12280). The above new microorganism was developed by collecting diseased black bream from various parts of Japan, isolating bacterial strains that are pathogenic to black bream, and culturing them in pure culture. It was created using a screening method to examine infectivity.
【０００６】上記新規微生物の菌学的性質の主なものを
列挙すると次の通りである。好気性菌であり、生育可能
なｐＨは６から８の範囲、生育温度は１５℃から３５℃
の範囲、生育適温は２５℃から３０℃の範囲である。ポ
テトデキストロース寒天培地（以下、「ＰＤＡ培地」と
略記する。）上において、集落の表面は乳白色であり、
同心円状に拡大する。また、底部に黒色の菌核が多量に
形成される。胞子は、白色または乳白色で、長さ約１０
マイクロメートル、幅約５マイクロメートルの紡錘状で
あり、鎖状に連結して、菌糸上に付着する。The main mycological properties of the above-mentioned new microorganism are listed below. It is an aerobic bacterium with a viable pH range of 6 to 8 and a growth temperature of 15°C to 35°C.
The suitable temperature for growth is in the range of 25°C to 30°C. On potato dextrose agar medium (hereinafter abbreviated as "PDA medium"), the surface of the colony is milky white,
Expand concentrically. Also, a large amount of black sclerotia is formed on the bottom. The spores are white or milky white and about 10 mm long.
They are spindle-shaped, about 5 micrometers wide, connected in a chain, and attached to the hyphae.
【０００７】菌核は、２日目に透明ガラス球状であった
ものが、３日目以降には黒色を呈し、きわめて堅い球状
をなす。その平均直径は約１７０マイクロメートルであ
るが、肉眼で識別可能な５００マイクロメートルを越す
大型のものも多く見られる。菌糸は、白色又は乳白色で
、幅約３マイクロメートル、約１００マイクロメートル
ごとに隔壁をもつ。プレート全面に集落が拡大するのに
７日から１０日で十分である。[0007] The sclerotia were transparent glass spheres on the second day, but after the third day they turned black and became extremely hard spheres. Their average diameter is about 170 micrometers, but there are many large ones exceeding 500 micrometers that can be discerned with the naked eye. The hyphae are white or milky white, about 3 micrometers wide, and have septa every 100 micrometers. Seven to ten days is sufficient for the colony to spread over the entire plate.
【０００８】また、本発明微生物のオートミル寒天培地
（以下、「ＯＭＡ培地」と略記する。）におけるコロニ
ーの形状と色調は、次の通りである。ＯＭＡ培地上にお
けるコロニーの形状と色調は、ＰＤＡ培地上と同様であ
り、裏面から観察したものも、表面から観察したものと
変化は見られない。[0008] Furthermore, the shape and color tone of colonies of the microorganism of the present invention on an oatmil agar medium (hereinafter abbreviated as "OMA medium") are as follows. The shape and color tone of the colonies on the OMA medium are the same as on the PDA medium, and no change is observed when observed from the back side and from the front side.
【０００９】これらの菌学的性質に基づき、ＤＥＭＡＴ
ＩＡＣＥＯＵＳ ＨＹＰＨＯＭＹ−ＣＥＴＥＳ（Ｍ．
Ｂ．Ｅｌｌｉｓ著、ＣＯＭＭＯＮＷＥＡＬＴＨ ＭＹ
ＣＯＬ−ＯＧＩＣＡＬ ＩＮＳＴＩＴＵＴＥ発行、１
９７１年）の記載を参照して、この微生物は、デンドリ
フィエラ属菌（Ｄｅｎｄｒｙｐｈｉｅｌｌａ属）である
と同定した。同菌の系統番号Ｋ−０４１を寄託番号：微
工研菌寄第１２２８０号として寄託した。本発明微生物
、デンドリフィエラ新属菌がクログワイから分離された
のは、世界的にはじめてである。Based on these mycological properties, DEMAT
IACEOUS HYPHOMY-CETES (M.
B. COMMONWEALTH MY by Ellis
Published by COL-OGICAL INSTITUTE, 1
This microorganism was identified as a genus Dendryphiella (Dendryphiella genus), with reference to the description in 1971). The strain number K-041 of the same bacterium was deposited as deposit number: FIKEN Bacteria No. 12280. This is the first time in the world that the microorganism of the present invention, a new genus Dendriphiella, has been isolated from Japanese black bream.
【００１０】また、この新規微生物は、水田雑草のクロ
グワイ、イヌホタルイ、タマガヤツリに病原性を有し、
イネ、コムギなどのイネ科植物、インゲンなどのマメ科
植物およびナス、トマトなどのナス科植物に病原性を示
さない。この新規微生物は、大量培養が可能であり、か
つ、容易に大量の胞子、菌核を得ることができる。[0010] In addition, this new microorganism is pathogenic to paddy field weeds such as black bream, cypress, and cypress.
It is not pathogenic to gramineous plants such as rice and wheat, legumes such as kidney beans, and solanaceous plants such as eggplants and tomatoes. This new microorganism can be cultured in large quantities, and large quantities of spores and sclerotia can be easily obtained.
【００１１】本発明の除草剤は、デンドリフィエラ属に
属する微生物を含有することを特徴とする。本発明の除
草剤は、一般に、同微生物を大量培養して得られる胞子
および菌核を水に懸濁したものを含む。The herbicide of the present invention is characterized in that it contains a microorganism belonging to the genus Dendriphiella. The herbicide of the present invention generally contains spores and sclerotia obtained by mass culturing the same microorganisms, suspended in water.
【００１２】本発明の雑草の防除方法は、前記、デンド
リフィエラ属に属する微生物または前記除草剤を用いる
ことを特徴とする。水田にクログワイが発生したときに
、同微生物をクログワイに接種するかまたは前記除草剤
を散布することによって、雑草を防除するものである。[0012] The weed control method of the present invention is characterized by using the above-mentioned microorganism belonging to the genus Dendriphiella or the above-mentioned herbicide. When black grubs occur in rice fields, the weeds are controlled by inoculating the black grubs with the microorganisms or spraying the herbicide.
【００１３】[0013]
【作用】本発明の新規微生物は、水田雑草であるクログ
ワイのほかイヌホタルイ、タマガヤツリにも病原性を有
し、潜伏期間が短く、激しい病徴を発現して、枯死させ
る。一方、イネ、コムギ、インゲン、ナス、トマトなど
の作物には、感染せず目標雑草に対する選択的な防除が
可能である。[Function] The novel microorganism of the present invention is pathogenic to the paddy field weeds, such as the Japanese black bream, as well as the Japanese cypress, and has a short incubation period, develops severe disease symptoms, and causes them to wither. On the other hand, crops such as rice, wheat, kidney beans, eggplant, and tomatoes are not infected and target weeds can be selectively controlled.
【００１４】[0014]
【実施例】１）本発明の新規微生物の創製手段水田に生
育し、かつ病徴を示すクログワイを採取し、その罹病部
分を約５ミリメートルの長さに切りとり、０．５％次亜
塩素酸ナトリウム溶液（Ｓｏｄｉｕ−ｍｈｙｐｏｃｈｌ
ｏｒｉｄｅ）に等量の０．１％ツィーン８０（Ｔｗｅｅ
ｎ８０）溶液を加えた溶液中に１分乃至３分間浸漬して
、表面殺菌を行なった。[Example] 1) Means for creating the novel microorganism of the present invention A Japanese black bream growing in a paddy field and exhibiting disease symptoms was collected, the diseased part was cut to a length of about 5 mm, and 0.5% hypochlorous acid was added to it. Sodium solution (Sodiu-mhypochl)
oride) and 0.1% Twee 80 (Twee
n80) solution was added for 1 to 3 minutes to perform surface sterilization.
【００１５】０．１％ツィーン８０溶液で１回洗浄、減
菌水で２回洗浄を行なった後、抗生物質入りポテトデキ
ストロース培地（以下、「ＡＰＤＡ」と略記する。）に
のせ、２５℃の恒温器中で菌糸を生育させ、その一部を
ＰＤＡ培地に移し、恒温器中で培養した。After washing once with 0.1% Tween 80 solution and twice with sterile water, it was placed on potato dextrose medium containing antibiotics (hereinafter abbreviated as "APDA") and incubated at 25°C. Mycelia were grown in a constant temperature chamber, a portion of which was transferred to a PDA medium, and cultured in a constant temperature chamber.
【００１６】また、水田より採取した病徴を示すクログ
ワイは、さく葉標本として保存可能であり、必要なとき
に病徴部分を切り取り、２５℃の湿室中に置くことによ
り、上記と同様に菌糸、胞子及び菌核が生育した。上記
いずれかの方法により生育した微生物を実体顕微鏡下で
単胞子分離を行ない、微生物の純粋分離を行なった。[0016] In addition, black bream showing disease symptoms collected from rice fields can be preserved as leaf specimens, and when necessary, by cutting off the diseased parts and placing them in a humid room at 25°C, they can be preserved in the same manner as above. Hyphae, spores and sclerotia grew. Microorganisms grown by any of the above methods were subjected to monospore separation under a stereomicroscope to isolate pure microorganisms.
【００１７】分離された各菌株について、クログワイに
対する病原性を再度検討するとともに、イネ、コムギ、
インゲン、ナス、トマトに対する病原性を検討し、クロ
グワイに対して優れた除草効果を発揮し、かつ、イネ、
コムギ、インゲン、ナス、トマトに病原性を示さない。
本発明微生物である系統番号Ｋ−０４１の菌株を分離し
た。[0017] Each isolated strain was reexamined for pathogenicity to black grub, and
We investigated its pathogenicity to green beans, eggplants, and tomatoes, and found that it had excellent herbicidal effects on green beans, eggplants, and tomatoes.
Not pathogenic to wheat, kidney beans, eggplants, and tomatoes. A strain of strain number K-041, which is a microorganism of the present invention, was isolated.
【００１８】２）創製した微生物の同定本発明微生物の
同定は、微生物をＰＤＡ培地で培養し、胞子、菌糸およ
び菌核の形態と生活史を観察して行なった。その結果、
前述の通り、本発明微生物はデンドリフィエラ属菌と同
定された。2) Identification of the created microorganism The microorganism of the present invention was identified by culturing the microorganism in a PDA medium and observing the morphology and life history of spores, hyphae, and sclerotia. the result,
As mentioned above, the microorganism of the present invention was identified as Dendriphiella.
【００１９】３）大量培養方法
ＰＤＡ培地上に、該微生物の胞子、菌糸または菌核を移
植し、２５℃明条件下に約１週間静置することにより、
容易に多量の胞子、菌核を得ることができる。該微生物
は、ペトリ皿１枚約１０７個の胞子すなわち約１０６個
の菌核の生産が可能であった。これらの方法により、大
量かつ効率的に生産された胞子の最高濃度は、５．２×
１０６個／ミリリットルであった。3) Mass culture method: By transplanting the spores, hyphae or sclerotia of the microorganism onto a PDA medium and leaving it for about a week under light conditions at 25°C,
A large amount of spores and sclerotia can be easily obtained. The microorganism was capable of producing about 10 7 spores or about 10 6 sclerotia per Petri dish. The highest concentration of spores produced in large quantities and efficiently by these methods was 5.2×
It was 106 pieces/ml.
【００２０】４）クログワイに対する病原力試験水田土
壌を充填した容積約１リットルのポットでクログワイを
生育させ約１か月経過したものを試験材料とした。本発
明微生物は、ＰＤＡ培地上で、生育させた胞子および菌
核を０．１％ツィーン８０溶液中に懸濁し、エアスプレ
ーを用いて、クログワイに接種を行なった後、湿室内で
２５℃に４８時間おき、２５℃の温室中で２週間後のク
ログワイの茎の発病率を調査し、その結果を表１に示し
た。4) Pathogenicity test for black-eyed bream The test material was a black-eyed bream grown in a pot with a volume of about 1 liter filled with paddy soil for about 1 month. The microorganism of the present invention is produced by suspending the spores and sclerotia grown on a PDA medium in a 0.1% Tween 80 solution, inoculating the black bream using air spray, and then heating the mixture to 25°C in a moist room. The disease incidence on the stems of the black bream was examined every 48 hours in a greenhouse at 25° C. after 2 weeks, and the results are shown in Table 1.
【００２１】[0021]
【表１】[Table 1]
【００２２】試験の結果、本発明微生物は、クログワイ
に対して優れた防除効果を示した。[0022] As a result of the test, the microorganism of the present invention showed an excellent control effect on black grub.
【００２３】５）他の雑草及び作物に対する影響他の雑
草及び作物に対する病原性試験は、クログワイに対する
病原力試験と同様の方法で行なつた。使用した接種源の
胞子濃度は１０６個／ミリリットル、菌核濃度は１０５
個／ミリリットルとした。イネは、２葉期、３葉期、４
葉期の３生育段階のものを用いた。その他コムギ、イン
ゲン、ナス、トマト、タマガヤツリ、ヒエを供試植物と
した。試験の結果を表２に示す。5) Effects on other weeds and crops Pathogenicity tests on other weeds and crops were carried out in the same manner as the pathogenicity test on black-eyed grouse. The spore concentration of the inoculum used was 106/ml, and the sclerotia concentration was 105.
units/ml. Rice has 2-leaf stage, 3-leaf stage, and 4-leaf stage.
Plants at three growth stages of the leaf stage were used. Other test plants were wheat, green beans, eggplants, tomatoes, Japanese cypress, and barnyard grass. The results of the test are shown in Table 2.
【００２４】[0024]
【表２】[Table 2]
【００２５】試験の結果、本発明微生物は、イネ、コム
ギ、インゲン、ナス、トマト、ヒエに病原性を示さなか
った。一方、クログワイ同様、主要水田雑草であるタマ
ガヤツリでは、葉がえ死し、イヌホタルイでは強度の生
育阻害が見られたことから、クログワイ、タマガヤツリ
、イヌホタルイを同時に防除することが可能である。As a result of the test, the microorganism of the present invention showed no pathogenicity to rice, wheat, kidney beans, eggplant, tomatoes, and barnyard grass. On the other hand, similar to the Japanese black bream, the leaves of the Japanese cypress, which is a major rice field weed, died and the growth of the Japanese firefly was severely inhibited. Therefore, it is possible to control the Japanese black porgy, the Japanese firefly, and the Japanese firefly at the same time.
【００２６】[0026]
【発明の効果】本発明微生物は、クログワイに対し選択
的に病原性を示し、イネなどの作物に影響を与えること
なく防除できる。また、化学合成農薬のように環境を汚
染・破壊することがない。EFFECTS OF THE INVENTION The microorganism of the present invention exhibits selective pathogenicity against black grub, and can control it without affecting crops such as rice. Also, unlike chemically synthesized pesticides, they do not pollute or destroy the environment.
Claims (3)
ｙｐｈｉｅｌｌａ新属菌）Ｋ−０４１（微工研菌寄第１
２２８０号）新規微生物。[Claim 1] Dendriphiella new genus (Dendr)
yphiella new genus) K-041
No. 2280) Novel microorganism.
含有することを特徴とする除草剤。2. A herbicide characterized by containing a microorganism belonging to the genus Dendriphiella.
用いることを特徴とする雑草の防除方法。3. A method for controlling weeds, which comprises using a microorganism belonging to the genus Dendriphiella.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3242900A JPH04368306A (en) | 1991-06-18 | 1991-06-18 | New fungus, fungus-containing herbicide and controlling weed thereby |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3242900A JPH04368306A (en) | 1991-06-18 | 1991-06-18 | New fungus, fungus-containing herbicide and controlling weed thereby |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04368306A true JPH04368306A (en) | 1992-12-21 |
Family
ID=17095891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3242900A Pending JPH04368306A (en) | 1991-06-18 | 1991-06-18 | New fungus, fungus-containing herbicide and controlling weed thereby |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH04368306A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994028723A1 (en) * | 1993-06-03 | 1994-12-22 | Japan Tobacco Inc. | Colletotrichum and use thereof |
| WO1998017780A1 (en) * | 1996-10-17 | 1998-04-30 | Japan Tobacco Inc. | Microorganism of the genus ascochyta and use thereof |
| US6750176B2 (en) | 2001-04-26 | 2004-06-15 | Kumiai Chemical Industry Co., Ltd. | Wettable compositions for use in agriculture, preparation method therefor, and storage method therefor |
| CN112955014A (en) * | 2018-11-01 | 2021-06-11 | 牧牛研究所株式会社 | Solid composition for managing plants comprising sclerotia of fungi and use thereof |
-
1991
- 1991-06-18 JP JP3242900A patent/JPH04368306A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994028723A1 (en) * | 1993-06-03 | 1994-12-22 | Japan Tobacco Inc. | Colletotrichum and use thereof |
| WO1998017780A1 (en) * | 1996-10-17 | 1998-04-30 | Japan Tobacco Inc. | Microorganism of the genus ascochyta and use thereof |
| US6750176B2 (en) | 2001-04-26 | 2004-06-15 | Kumiai Chemical Industry Co., Ltd. | Wettable compositions for use in agriculture, preparation method therefor, and storage method therefor |
| CN112955014A (en) * | 2018-11-01 | 2021-06-11 | 牧牛研究所株式会社 | Solid composition for managing plants comprising sclerotia of fungi and use thereof |
| CN112955014B (en) * | 2018-11-01 | 2023-07-28 | 牧牛研究所株式会社 | Solid composition for managing plants comprising sclerotium of a fungus and use thereof |
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