JPH07108848B2 - Method for controlling plant diseases using symbiotic microorganisms and novel microorganisms used for implementing the method - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION Object of the Invention (Field of Industrial Application) The present invention relates to a method for controlling plant diseases utilizing the antagonism of symbiotic microorganisms and a novel microorganism used for carrying out the method.

(Prior Art) To the best knowledge of the present inventors, a microorganism symbiotic in the disease-free tissue of cyclamen is separately cultivated, and seedlings showing affinity are inoculated, and the pathogenic bacteria are eradicated by its antagonistic action by the growth. No possible technology for controlling plant diseases has been proposed or implemented so far.

(Problems to be Solved by the Invention) The present inventors, in the stage of advancing research on aseptic culture of cyclamen and sweet potato, that bacteria and filamentous fungi coexist in the disease-free tissue of cyclamen, its symbiotic site is Roots, tubers,
Petiole, leaf blade, inflorescence, sepal, not found in seeds, aseptic culture of cyclamen enables aseptic culture (successful experiment), and It was found that when seedlings of different plants were exposed to natural conditions, they returned to the original symbiotic state of bacteria and filamentous fungi (cyclamen always have symbiotic microorganisms in their tissues).

By the way, since the sterile culture seedlings propagate a single individual, the genetic traits are also the same. This same trait is necessary in tissue culture, but as a disadvantage, it shows the same resistance reaction to disease, so once the occurrence of disease is encountered, the genetic trait is the same as described above, In some cases, it will be wiped out.

Therefore, it is an indispensable subject to impart disease resistance to such sterile culture seedlings.

The present inventors proceeded further research from the above circumstances, focusing on the fact that cyclamen always have a symbiotic microorganism in the tissue as described above, separating useful microorganisms symbiotic in the disease-free tissue, and It was conceived and realized to inoculate a sterile culture seedling to impart resistance.

Further, it has been clarified that the useful microorganisms involved in this are all novel microorganisms.

[Means for Solving the Problem] (Regarding Control Method) That is, the present invention is to separate microorganisms that co-exist in the disease-free tissue of cyclamen separately, and to separate one or more of the microorganisms that have been separately cultivated. A method for controlling plant diseases using a symbiotic microorganism, which is capable of controlling pathogens by inoculating general cultivated seedlings or aseptic cultivated seedlings of sexually active organisms and antagonizing the growth of the cyclamen.

And, in the above, a method for controlling plant diseases using a symbiotic microorganism, which is a symbiotic microorganism in the asymptomatic tissue of cyclamen, is Erwinia CE-057 (Microtechnology Research Institute No. 11630) strain.

And also in the above, the microorganisms symbiotic in the asymptomatic tissue of cyclamen are Pseudomonas CP-098.
(Ministry of Microbiological Research, No. 11629) A method for controlling plant diseases using a symbiotic microorganism that is a strain.

Further, in the above, a method for controlling plant diseases using a symbiotic microorganism, which is a Bacillus CB-131 (Microtechnological Research Institute No. 11625) strain, is a symbiotic microorganism in the symptom-free tissue of cyclamen.

(About symbiotic microorganisms) And it is a symbiotic bacterium in healthy cyclamen's asymptomatic tissues.
A yellow pigment that can be cultivated and that when the cultivated fungus is inoculated into a general cultivated seedling of cyclamen or a sterile cultivated seedling, it shows affinity in the tissue of cyclamen and can settle, and is non-pathogenic to cyclamen. Of Escherichia coli (Er) (Er), which is negative for the production of N, nitrate reduction and hydrogen sulfide, and negative for indole production and decarboxylase activity.
winia) CE-057 (Microtechnology Research Institute No. 11630) strain is a symbiotic bacterium which is a symbiotic bacterium that is symbiotic as a novel bacterium and in healthy cyclamen's disease-free tissues. It can be easily separated and cultivated, and when the cultivated fungus is inoculated into a general cultivated seedling of Cyclamen or a sterile cultivated seedling
Nitrate reduction, which has an affinity and colonization in cyclamen tissues and is non-pathogenic to cyclamen, is positive, hydrogen sulfide production, indole production, arginine hydrolysis, oxidase (Pseudomona (Pseudomona), which is negative for the activity of Pseudomonas and the production of fluorescent dye
s) CP-098 (Ministry of Industrial Science and Technology No. 11629) is a bacterium that is a symbiotic bacterium in a healthy cyclamen-free symptom-free tissue as a novel microbial organism. It can be easily separated and cultivated, and when the cultivated bacteria are inoculated into cyclamen's general cultivated seedlings or aseptic cultivated seedlings, they show an affinity in the tissue of cyclamen and can settle, and it is nonpathogenic to cyclamen. There is a positive reduction of nitrate, hydrogen sulfide production, indole production,
The activity of decarboxylase and the hydrolysis of casein are negative. Bacillus CB-131
No. 1625), a symbiotic microorganism as a novel microorganism, is also claimed.

Looking at the bacteria (symbiotic microorganisms) isolated from within the asymptomatic tissues of each part of cyclamen in terms of seedlings and clones,
The results are shown in Tables 1 and 2 below.

Microorganisms isolated as described above are Ps in cyclamen.
As Pseudomonas, which belongs to eudomonas, Erwinia, and Bacillus, and whose resistance is imparted by inoculating cyclamen, CP-098
No.), and in Erwinia CE-057 (Microtechnical Research Institute No. 11630), B
In acillus, CB-131 (Ministry of Industrial Science, No. 11625) was selected, and it was revealed that they are novel microorganisms because they all have the bacteriological properties described separately.

[Effects of the Invention] As a result of carrying out the present invention using these isolated microorganisms, the effects of disease control were fully exhibited.

That is, most of these strains showed an affinity for general-cultivated and aseptic tissue culture cyclamen and no pathogenicity. When these were inoculated into cyclamen, they showed resistance to anthracnose and suppressed the onset of anthracnose subsequently inoculated.

The results of the pest control test are shown in the table below.

<Results of control test for anthracnose> As described above, the present invention is of great significance in that the indispensable problem of imparting disease resistance to aseptic cultured seedlings is solved by the biological method of separating and inoculating symbiotic microorganisms.

Continuation of the front page (51) Int.Cl. ⁶ Identification code Office reference number FI Technical display part (C12N 1/20 C12R 1:38) (C12N 1/20 C12R 1:07) (C12N 1/20 C12R 1: 64)

Claims (7)

[Claims] 1. A cyclamen cultivated seedling or a cultivated seedling of a cyclamen which has an affinity for these microorganisms is inoculated with the microorganisms coexisting in the symptom-free tissue of cyclamen, which are individually separated. Then, a method for controlling plant diseases using a symbiotic microorganism capable of exterminating pathogenic bacteria by antagonizing its growth. 2. A symbiotic microorganism of cyclamen in the asymptomatic tissue is Erwinia CE-057
No. 11630), a method for controlling plant diseases using the symbiotic microorganism according to claim 1. 3. The symbiotic microorganism according to claim 1, wherein the symbiotic microorganism in the asymptomatic tissue of cyclamen is Pseudomonas CP-098 (Microtechnical Research Institute No. 11629) strain. For controlling plant diseases. 4. The microorganism symbiotic in the asymptomatic tissue of cyclamen is Bacillus CB-131 (Microtechnical Research Institute
No. 25) strain, a method for controlling plant diseases using the symbiotic microorganism according to claim 1. 5. A symbiotic bacterium that coexists in healthy cyclamen's disease-free tissue, which can be easily separated and cultivated by a conventional method such as NA medium, and the cultivated bacterium can be a general cyclamen cultivated seedling or a sterile cultivated seedling. When inoculated into, cyclamen shows an affinity within the tissue, is capable of colonization, and is nonpathogenic to cyclamen, positive for the production of yellow pigment, reduction of nitrate and production of hydrogen sulfide, and indole. , A new microorganism which is Erwinia CE-057 (Microtechnology Research Institute No. 11630) strain, which is negative for the production of Escherichia coli and decarboxylase activity. 6. A bacterium that co-exists in healthy cyclamen's asymptomatic tissue, and can be easily separated and cultured from this by an ordinary method such as NA medium, and the cultivated bacterium can be a general cyclamen cultivated seedling or a sterile culture seedling. When inoculated into, cyclamen shows an affinity within the tissue, is capable of colonization, and is nonpathogenic to cyclamen, the reduction of nitrate is positive,
A novel microorganism, which is Pseudomonas CP-098 (Microtechnology Research Institute No. 11629) strain, which is negative in hydrogen sulfide production, indole production, arginine hydrolysis, oxidase activity, and fluorescent dye production. 7. A bacterium that co-exists in healthy cyclamen's asymptomatic tissue, and can be easily separated and cultured from this by an ordinary method such as NA medium, and the cultivated bacterium can be a general cyclamen cultivated seedling or a sterile cultivated seedling. When inoculated into, cyclamen shows an affinity within the tissue, is capable of colonization, and is nonpathogenic to cyclamen, the reduction of nitrate is positive,
A novel microorganism, which is Bacillus CB-131 (Microtechnical Research Institute No. 11625) strain, which is negative in hydrogen sulfide production, indole production, decarboxylase activity and casein hydrolysis.