JP2654931B2 - Method for controlling plant diseases using symbiotic microorganisms and novel microorganisms used for implementing the method - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for controlling plant diseases utilizing the antagonism of symbiotic microorganisms, and a novel microorganism used for carrying out the method.

[0002]

2. Description of the Related Art To the best of our knowledge, microorganisms that coexist in symptomatic tissues of sweet potato are isolated and cultured, and inoculated into seedlings exhibiting affinity. Techniques for controlling plant diseases that have been made possible have not been proposed or implemented.

[0003]

SUMMARY OF THE INVENTION The inventors of the present invention, at the stage of researching aseptic culture of sweet potato and cyclamen, confirmed that bacteria and filamentous fungi coexist in the asymptomatic tissue of sweet potato, and that the symbiotic site is Roots, tubers, petiole, leaf blade, stalks, sepals, but not found in seeds, aseptic cultivation of sweet potato by aseptically cultivating the seeds (successful experiment), and When exposed to sterile conditions, the seedlings of plants that had been aseptically cultured returned to the original symbiotic state of bacteria and fungi (sweet potatoes always have symbiotic microorganisms in their tissues).

[0004] Since aseptic culture seedlings propagate a single individual, they have the same genetic traits. This same trait is necessary in tissue culture, but as a disadvantage, because it shows the same resistance response to disease, once the occurrence of the disease is encountered, the genetic trait is the same as described above, In some cases, it will be annihilated. Therefore, it is essential to impart disease resistance to such sterile cultured seedlings.

[0005] The present inventors have further researched from the above-mentioned circumstances, and focused on the fact that sweet potatoes always have symbiotic microorganisms in tissues as described above, and isolated useful microorganisms that coexist in symptomatic tissues. The inventor of the present invention has conceived and realized the concept of inoculating this into a sterile cultured seedling to impart resistance. Further, it has been clarified that all useful microorganisms involved in this are novel microorganisms.

[0006]

[Means for Solving the Problems]

(Regarding the control method) That is, the present invention separates microorganisms coexisting in the asymptomatic tissue of sweet potato separately, and singly or plurally isolates and cultures of the microorganisms into a general cultivated seedling of sweet potato or This is a method for controlling plant diseases using a symbiotic microorganism which inoculates aseptic cultured seedlings and enables the elimination of pathogenic bacteria by antagonism due to their growth.

Among the above, microorganisms symbiotic in the asymptomatic tissue of sweet potato are Pseudomonas (Pseudomon).
onas) A method for controlling plant diseases using a symbiotic microorganism which is a strain BP-084 (Microorganism No. 11627 shown in the attached accession number). And again,
Control of plant diseases using symbiotic microorganisms in which the microorganisms symbiotic in the asymptomatic tissue of the sweet potato are Bacillus BB-145 (Microorganisms No. 11626 No. 11626 indicated on the attached accession certificate) Is the way. Further, among the above, a microorganism symbiotic in the asymptomatic tissue of sweet potato is Xanthomonas B
This is a method for controlling plant diseases using a symbiotic microorganism which is a strain X-146 (No. 11628, a microbiological laboratory strain indicated in the attached deposit certificate).

(Symbiotic microorganisms) Bacteria which coexist in healthy sweet potato asymptomatic tissues, which can be easily separated and cultured by a conventional method such as an NA medium, and the cultured bacteria can be used for general cultivation of sweet potato seedlings. Alternatively, when inoculated into a sterile cultured seedling, it shows affinity in the tissues of the sweet potato, is capable of colonization, and is non-pathogenic to the sweet potato. The reduction of nitrate is positive, the production of hydrogen sulfide, Pseudomonas BP-084 (Microtechnical Laboratories No. 11627) strain which is negative for the production of arginine, the hydrolysis of arginine, the activity of oxidase, and the production of fluorescent dye is a novel microorganism As

Also, it is a bacterium that coexists in the disease-free tissue of healthy sweet potato, which can be easily separated and cultured by a conventional method such as NA medium, and the cultivated bacteria can be used as a general cultivated seedling or aseptically cultured seedling of sweet potato. When inoculated in the potato, it shows affinity in the tissues of the sweet potato and can be established,
Bacillus BB-145, which is positive for nitrate reduction and non-pathogenic to sweet potato, and negative for hydrogen sulfide production, indole production, decarboxylase activity and urease activity. Symbiotic microorganisms that are strains

[0010] Furthermore, it is a bacterium that coexists in the disease-free tissue of healthy sweet potato, which can be easily separated and cultured by a conventional method such as NA medium, and the cultivated bacteria can be used for general cultivated seedlings or aseptically cultured seedlings of sweet potato. When inoculated, it shows affinity in the tissues of the sweet potato and can be established,
The production of yellow pigment, reduction of nitrate and production of hydrogen sulfide, which are nonpathogenic to sweet potato, are positive, and
The present invention also claims, as a novel microorganism, a symbiotic microorganism which is a strain of Xanthomonas BX-146 (No. 11628), which is negative for the hydrolysis of casein.

The following Tables 1 and 2 show the bacteria (symbiotic microorganisms) isolated from the asymptomatic tissues of each part of the sweet potato.

[0012]

Table 1 Bacteria isolated from sweet potatoes (1 to 5)

[0013]

[Table 2] Bacterial species isolated from each part of sweet potato

The microorganism isolated as described above is Pse
udomonas, Xanthomonas, Baci
lpus, which is given resistance by inoculating sweet potatoes, as Pseudomo
For nas, as shown in Table 3 below, BP-084 (No. 11627), and for Xanthomonas, as shown in Table 4 below, BX-146 (No. 116).
No. 28), Bacillus selected BB-145 (No. 11626) from Bacillus, as shown in Table 5 below, and all of them had the bacteriological properties described separately, indicating that they were novel microorganisms. It became.

[0015]

[Table 3]

[0016]

[Table 4]

[0017]

[Table 5]

[0018]

Effects of the Invention As a result of carrying out the present invention using these isolated microorganisms, the effect of disease control was fully exhibited. That is, most of these strains showed affinity for sweet potatoes in general cultivation and sterile tissue culture, and did not show pathogenicity. Inoculation of sweet potatoes with these showed resistance to wilt and reduced the incidence of subsequently vaccinated wilt. The results of the control test are shown in Table 6 below.

[0019]

[Table 6] As described above, the present invention is of great significance in that the indispensable problem of imparting disease resistance to sterile cultured seedlings is solved by a biological method of isolating and inoculating symbiotic microorganisms.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification number Agency reference number FI Technical display location C12R 1:07) (C12N 1/20 C12R 1:64)

Claims (7)

(57) [Claims] 1. Microorganisms symbiotic in the disease-free tissue of sweet potato are separately isolated, and one or more of the microorganisms isolated and cultured are inoculated to a general cultivated seedling or a sterile cultured seedling of sweet potato exhibiting an affinity for them. And a method for controlling plant diseases using a symbiotic microorganism which can control pathogenic bacteria by antagonizing the growth. 2. The microorganism symbiotic in the asymptomatic tissue of sweet potato is Pseudomonas B.
The method for controlling a plant disease using a symbiotic microorganism according to claim 1, which is a strain P-084 (Micro-organism No. 11627). 3. The microorganism symbiotic in the asymptomatic tissue of sweet potato is Bacillus BB-145.
The method for controlling a plant disease using the symbiotic microorganism according to claim 1, which is a strain (Piercian Laboratories No. 11626). 4. The microorganism symbiotic in the asymptomatic tissue of sweet potato is Xanthomonas B.
The method for controlling a plant disease using a symbiotic microorganism according to claim 1, which is a strain X-146 (Microtechnological Laboratory No. 11628). 5. A bacterium that coexists in the asymptomatic tissue of healthy sweet potato, which can be easily separated and cultured by an ordinary method such as NA medium, and the cultivated bacterium is used as a general cultivated seedling or aseptically cultured seedling of sweet potato. When inoculated, the potatoes show affinity in the tissues of the sweet potato, are capable of colonization, and are nonpathogenic to the sweet potato, are positive for the reduction of nitrate, produce hydrogen sulfide, produce indole, Pseudomonas B negative for arginine hydrolysis, oxidase activity and fluorescent dye production
A novel microorganism which is P-084 (Microbial Lab. No. 11627) strain. 6. Bacteria that coexist in the disease-free tissues of healthy sweet potatoes, which can be easily separated and cultured by a conventional method such as NA medium, and the cultured bacteria can be used for general cultivation of sweet potato or aseptic culture. When seedlings are inoculated, they show affinity in the tissues of the sweet potato, are capable of colonization, and are nonpathogenic to the sweet potato, positive for nitrate reduction, hydrogen sulfide production, indole production A new microorganism which is a Bacillus BB-145 strain (No. 11626, Bacillus Bacillus) having negative carboxylase and urease activities. 7. A bacterium which coexists in the asymptomatic tissue of healthy sweet potato, which can be easily separated and cultured by a conventional method such as NA medium, and the cultivated bacteria can be used as a general cultivated seedling or aseptically cultured seedling of sweet potato. When inoculated into potatoes, it shows affinity in the tissues of the sweet potato, is capable of colonization, and is non-pathogenic to the sweet potato, positive for yellow pigment production, nitrate reduction and hydrogen sulfide production, and A novel microorganism which is a strain of Xanthomonas BX-146 (No. 11628, manufactured by B.I.K.) which is negative for the hydrolysis of casein.