JPH0372083B2 - - Google Patents
Info
- Publication number
- JPH0372083B2 JPH0372083B2 JP20129286A JP20129286A JPH0372083B2 JP H0372083 B2 JPH0372083 B2 JP H0372083B2 JP 20129286 A JP20129286 A JP 20129286A JP 20129286 A JP20129286 A JP 20129286A JP H0372083 B2 JPH0372083 B2 JP H0372083B2
- Authority
- JP
- Japan
- Prior art keywords
- amylase
- units
- enzyme
- polymer powder
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 36
- 239000000126 substance Substances 0.000 claims description 27
- 239000000463 material Substances 0.000 claims description 19
- 238000006243 chemical reaction Methods 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 238000004132 cross linking Methods 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 7
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 claims description 6
- 238000006116 polymerization reaction Methods 0.000 claims description 5
- 239000003431 cross linking reagent Substances 0.000 claims description 4
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 description 63
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 61
- 102000004190 Enzymes Human genes 0.000 description 55
- 108090000790 Enzymes Proteins 0.000 description 55
- 229940088598 enzyme Drugs 0.000 description 55
- 108010019077 beta-Amylase Proteins 0.000 description 54
- 229920000642 polymer Polymers 0.000 description 45
- 108090000637 alpha-Amylases Proteins 0.000 description 41
- 102000004139 alpha-Amylases Human genes 0.000 description 41
- 229940024171 alpha-amylase Drugs 0.000 description 41
- 239000007788 liquid Substances 0.000 description 36
- 239000000706 filtrate Substances 0.000 description 35
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 27
- 230000000694 effects Effects 0.000 description 26
- 239000000243 solution Substances 0.000 description 20
- 239000004382 Amylase Substances 0.000 description 15
- 102000013142 Amylases Human genes 0.000 description 15
- 108010065511 Amylases Proteins 0.000 description 15
- 235000019418 amylase Nutrition 0.000 description 15
- 239000006228 supernatant Substances 0.000 description 11
- 108091005804 Peptidases Proteins 0.000 description 10
- 238000003795 desorption Methods 0.000 description 10
- 238000000034 method Methods 0.000 description 10
- 238000003756 stirring Methods 0.000 description 10
- 239000011550 stock solution Substances 0.000 description 10
- 238000005406 washing Methods 0.000 description 10
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 9
- 239000004365 Protease Substances 0.000 description 9
- 235000011121 sodium hydroxide Nutrition 0.000 description 9
- 239000003463 adsorbent Substances 0.000 description 8
- 229920001542 oligosaccharide Polymers 0.000 description 8
- 150000002482 oligosaccharides Chemical class 0.000 description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 7
- 229920000856 Amylose Polymers 0.000 description 6
- 240000006439 Aspergillus oryzae Species 0.000 description 6
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 238000011084 recovery Methods 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 238000004040 coloring Methods 0.000 description 5
- 230000003247 decreasing effect Effects 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 239000005416 organic matter Substances 0.000 description 5
- 238000010992 reflux Methods 0.000 description 5
- 238000001179 sorption measurement Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 229920006037 cross link polymer Polymers 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 239000002861 polymer material Substances 0.000 description 3
- 235000011118 potassium hydroxide Nutrition 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 241000228212 Aspergillus Species 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000235527 Rhizopus Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000005185 salting out Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- LUEWUZLMQUOBSB-UHFFFAOYSA-N UNPD55895 Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(OC3C(OC(O)C(O)C3O)CO)C(O)C2O)CO)C(O)C1O LUEWUZLMQUOBSB-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006103 coloring component Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000003262 industrial enzyme Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- UYQJCPNSAVWAFU-UHFFFAOYSA-N malto-tetraose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(O)C(CO)O2)O)C(CO)O1 UYQJCPNSAVWAFU-UHFFFAOYSA-N 0.000 description 1
- LUEWUZLMQUOBSB-OUBHKODOSA-N maltotetraose Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O[C@@H]3[C@@H](O[C@@H](O)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-OUBHKODOSA-N 0.000 description 1
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 238000002336 sorption--desorption measurement Methods 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1
Landscapes
- Polyethers (AREA)
- Enzymes And Modification Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20129286A JPS6357601A (ja) | 1986-08-29 | 1986-08-29 | 三次元架橋化高分子物質及びその製造方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20129286A JPS6357601A (ja) | 1986-08-29 | 1986-08-29 | 三次元架橋化高分子物質及びその製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6357601A JPS6357601A (ja) | 1988-03-12 |
| JPH0372083B2 true JPH0372083B2 (enrdf_load_stackoverflow) | 1991-11-15 |
Family
ID=16438561
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20129286A Granted JPS6357601A (ja) | 1986-08-29 | 1986-08-29 | 三次元架橋化高分子物質及びその製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6357601A (enrdf_load_stackoverflow) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3204143B2 (ja) * | 1997-01-06 | 2001-09-04 | 日本電気株式会社 | ディスクキャッシュの制御方法 |
| DE10022095B4 (de) * | 2000-05-08 | 2005-07-14 | Südzucker AG Mannheim/Ochsenfurt | Gel aus einem Poly-α-1,4-Glucan und Stärke |
-
1986
- 1986-08-29 JP JP20129286A patent/JPS6357601A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6357601A (ja) | 1988-03-12 |
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