JPH03218302A - Reproducing agent for seaweeds - Google Patents

Abstract

PURPOSE:To obtain a reproducing agent for seaweeds containing at least a species of organic acid selected from the group of citric acid, isocitric acid, oxaloacetic acid, malic acid, succinic acid, ketoglutaric acid, glutamic acid and glyoxylic acid and/or salt of said organic acid. CONSTITUTION:At least a species of organic acid selected from the group of citric acid, isocitric acid, oxaloacetic acid, malic acid, succinic acid, ketoglutaric aoid, glutamic acid and glyoxylic acid and/or salt of said organic acid is contained in the objective reproducing agent for seaweeds accelerating the growth of seaweeds constructing sea weed bed or seaweed field useful as a place of living, spawning and growing of seaweeds usable for food such as ear-shell, top shell, sea urchin, small snails and fishes, etc. Said essential ingredient is mixed with a resin, etc., and preferably used in applying on a base material. Seaweeds are effectively propagated with said application.

Description

Detailed Description of the Invention (Field of Industrial Application) The present invention is directed to a seaweed bed useful as a habitat, spawning, and growing place for edible seaweed, abalone, turban shell, sea urchin, small snails, fish, etc. This invention relates to a seaweed propagation agent that promotes the growth of seaweeds that make up underwater forests.

(Prior art) In general, for cultivating edible seaweed and creating seaweed beds and underwater forests, a method is used to produce seedlings of young seaweed, kelp, arame, sargassum, sargassum, etc., and transplant them to the seabed. However, it takes a long time to cultivate seaweed seeds. For this reason, common nutritional substances, such as phosphates, nitrates, ammonium salts, vitamins, fishmeal, and oil scum, are added to seawater and used as nutritional supplements in the cultivation of seeds and seedlings. However, a satisfactory effect has not been obtained.

(Problems to be Solved by the Invention) The present invention aims to improve seaweeds and seedlings that constitute seaweed beds, underwater forests, etc. for the purpose of maintaining seaweeds that are useful as marine resources and useful marine resources in coastal waters. It is a composition that promotes proliferation and growth, and can be added to seaweed cultivation equipment, such as culture tanks, or mixed with resin, etc., to serve as a substrate for seaweed growth, such as films, vinyl chloride boards, rocks, concrete blocks, concrete plates, etc. By applying it to artificial reefs, artificial fish reefs, etc., it promotes the growth of seaweed and solves the conventional problems.

(Means for solving the problem) The present invention provides at least one organic acid and/or organic acid selected from the group consisting of citric acid, isocitric acid, oxaloacetic acid, malic acid, succinic acid, ketoglutaric acid, glutamic acid, and glyoxylic acid. The present invention relates to a seaweed growth agent characterized by containing salt.

Examples of organic acids that can be used in the present invention include citric acid, isocitric acid, oxaloacetic acid, malic acid,
At least one selected from the group consisting of succinic acid, ketoglutaric acid, glutamic acid, and glyoxylic acid can be mentioned.

The organic acid salts that can be used in the present invention include sodium salts of at least one organic acid selected from the group consisting of citric acid, isocitric acid, oxaloacetic acid, malic acid, succinic acid, ketoglutaric acid, glutamic acid, and glyoxylic acid; potassium salt, calcium salt, magnesium salt,
It is at least one selected from the group consisting of iron salts, ammonium salts, manganese salts, molybdenum salts, and cobalt salts.

In addition, common nutritional substances such as amino acids, phosphates, silicates, nitrates, ammonium salts, vitamins,
Minerals, fishmeal, oil and the like may be used in combination.

The essential components of the present invention can be used by blending them into a resin or the like and applying them to a base material. The resin used may be any resin that can dissolve or disperse the essential components, and examples thereof include vinyl chloride resins, acrylic resins, polypropylene resins, polyester resins, and the like. These resins are appropriately selected depending on their adhesion to the base material, compatibility with essential components, and the like.

0.0 when adding the essential components of the present invention to the culture tank
1 to 5000 ppm, preferably 0, 1 to 500 ppm
Add to a concentration of . If the concentration is less than 0.001 ppm, no growth effect will be obtained, and if the concentration exceeds 5000 ppm, the algae growth rate will be slowed down, which is not preferable.

When the essential components of the present invention are blended into a resin or the like, the blending composition is in the range of 0.01 to 100 parts by weight, preferably 0.01 to 10 parts by weight, per 1 part by weight of the resin. Furthermore, when adding other general nutritional substances, it is preferably in the range of 0.1 to 10 parts by weight per 1 part by weight of the essential ingredients.

The composition blended with the resin etc. of the present invention is a fishing reef installed in the sea for the purpose of forming a seaweed bed or underwater forest that is useful as a habitat, spawning, and growing place for abalone, turban shell, sea urchin, small snails, fish, etc. For example, it can be applied to concrete, iron, steel, plastic, rubber, rock, etc.

Examples of the seaweeds used in the present invention include green algae of the order Ulva, Corinthidae, Myriale, red algae of the order Apocalyptales, Cyzomenales, Amanitales, Cucleatales, Urinariales, Agiformes, and brown algae of the Order Nagamidae. Cyperoptera,
Examples include Laminariaformes, Laminariales, etc. Examples of green algae include the order Ulva, such as Algae, Ulva, Urticaria, and Iwa5. Orders such as Amanita, Amanita, and Agaricales, such as Amanita, Agarina, and Agaricales, such as Amanita, Agarina, and Agarina, and brown algae such as Amanita, Agarina, Mozuku, Amaranth, Amaranth, Amaranth, Amaranth, Amaranth, Amaranth, Amaranth, Amaranth, etc. , kelp, wakame, and members of the order Fucus, such as porcupine, porphyry, sargassum, sargassum, Japanese strawberry, Japanese staghorn, Japanese trifoliate, Japanese porphyry, and sawfish.

(Function) In the present invention, organic acids and/or organic acid salts are added directly to aquaculture equipment for marine useful algae, such as culture tanks, or are incorporated into resins, etc., and applied to concrete blocks, rocks, artificial reefs, etc. This allows algae to grow efficiently. In other words, it is effective as a seaweed propagation agent that can promote the growth of useful algae and maintain useful marine resources in coastal waters.

(Example) Hereinafter, embodiments of the present invention will be specifically illustrated and described with reference to Examples. The present invention is not limited to these examples.

Example 1 200 [In a 111-volume culture flask, 500 μg/sodium malate was added as a growth agent to basic culture water and seawater.
Pour 200 ml of the culture solution prepared by adding 200 ml of culture solution, add 3 individuals of Arame algae (stem cut into 31 TIIn, leaf cut into 15+w+n size) into the solution, and place in a constant temperature chamber at 15°C. Aerated culture was carried out for 10 days under conditions of illuminance of 5,500 Lx and 12 hours of illumination per day.

Each culture solution was replaced every two days, and the wet weight after culture was measured.
The weight at the start of culture was taken as 1, and the weight ratio was determined and the growth was compared. The results are shown in Table-1.

Comparative Example 1 Add 200ml of basic culture water to a 200ml culture flask.
0 ml, and put 3 algal bodies similar to those in Example 1 into this, and in a constant temperature area of 15°C, illuminance of 5500LX, once a day.
Aerated culture was performed for 10 days under the condition of 2 hours of illumination. The culture solution was exchanged every two days, the wet weight after culture was measured, and the weight ratio was calculated with the weight at the start of culture as 1 to compare the growth. The results are shown in Table-1.

Table 1 Example 2 Into a 200 ml culture flask, put 200 ml of a culture solution prepared by adding monosodium glutamate as a growth agent to 300 μg/m+ to basic culture super-seawater, and add wakame algae (stems) to the culture flask. The leaves were cut into 3 pieces and the leaves were cut into 15 mm pieces), and 3 individuals were cultured for 10 days in a constant temperature room at 15°C under conditions of illumination of 5500 Lx and 12 hours a day.

Each culture solution was replaced every two days, and the wet weight after culture was measured.
The weight at the start of culture was taken as 1, and the weight ratio was determined and the growth was compared. The results are shown in Table-2.

Comparative Example 2 Put 200 ml of basic culture water in a 200 ml culture flask, add 3 alga bodies similar to those in Example 2, and heat at a constant temperature of 15°C with an illuminance of 5500 LX.
Aerated culture was performed for 10 days under the condition of 12 hours of light per day. The culture solution was replaced every two days, and the wet weight after culture was measured.
The weight at the start of culture was taken as 1, and the weight ratio was determined and the growth was compared. The results are shown in Table-29.

10 Demonstration Example 3 In a 200 ml culture flask, add 100 μg/ml of sodium malate as a growth agent to the basic culture water and seawater.
Pour 200 + nl of a culture solution prepared by adding 10 μg/rnl of oxalo#acid and 50 μg/ml of sodium citrate, and add the alga bodies (leaves cut into 101 m long pieces) of A. ) Five individuals were placed in a constant temperature zone at 15°C, and aerated culture was carried out for 10 days under the conditions of illumination intensity of 5500Lx and lighting for 12 hours a day. Each culture solution was exchanged every two days, the area of the thallus after culture was measured, and the area ratio was determined with the area at the start of culture being 1, and the growth was compared. The results are shown in Table-3.

Comparative Example 3 200 ml of basic cultured seawater was put into a 200 ml culture flask, and 5 alga bodies similar to those in Example 3 were placed therein at a constant temperature of 15°C, with an illuminance of 5500 LX, once a day.
Aerated culture was performed for 10 days under the condition of 2 hours of illumination. The culture solution was exchanged every two days, and the area of 11 leaflets after culture was measured, and the area ratio was determined with the area at the start of culture as 1, and the growth was compared.

Display results Shown in 3.

Table 3 12 Examples 4 to 6 5, 50, 500 μg of citric acid was added as a growth agent to the basic culture solution and seawater in 200 ml culture flasks, respectively.
/ ml of culture solution prepared by adding 20
0 ml, and put 3 individuals of Akamoku algae (stem including the apical part where the growth point is cut into 15 body size pieces) in this, and keep it in a constant temperature area at 15℃ with illuminance of 5500Lx.
Aerated culture was carried out for 10 days under the condition of 12 hours of illumination per day. Each culture solution was exchanged every two days, the wet weight after culture was measured, the weight at the start of culture was taken as 1, the weight ratio was determined, and the growth was compared. The results are shown in Table 4.

Comparative Example 4 200 ml of basic cultured seawater was placed in a 200 ml culture flask, 3 algae similar to that in Example 4 were placed therein, and the temperature was kept constant at 15°C with an illuminance of 5500 LX.
Aerated culture was carried out for 10 days under the condition of 12 hours of illumination per day. The culture solution was replaced every two days, and the wet weight after culture was measured.
Taking the weight at the start of culture as 1, the weight ratio was determined and the growth was compared.

The results are shown in Table 9. Table 4 14 Examples 7 to 9 200 ml of the same culture solution as in Examples 4 to 6 as a propagation agent was placed in each 200 cal culture flask, and Three individuals of Yatsumatamoku algae (stem including the nuchal end where the growth point is cut into 15-inch pieces) were placed in a constant temperature area of 15°C with an illuminance of 5500LX for 12 days a day.
Aerated culture was performed for 10 days under the condition of timed lighting. Each culture solution was exchanged every two days, the wet weight after culture was measured, the weight at the start of culture was taken as 1, the weight ratio was determined, and the growth was compared. The results are shown in Table 5.

Comparative Example 5 200 ml of basic culture r supersea water was put into a 200 ml culture flask, and 3 algae similar to those in Example 7 were placed therein.
Aerated culture was carried out for 10 days under the condition of 12 hours of illumination per day. The culture solution was replaced every two days, and the wet weight after culture was measured.
The weight at the start of culture was taken as 1, and the weight ratio was determined and the growth was compared. The results are shown in Table-515.

Table 5 1 6 Example 10 Into a 200 ml culture flask, put 200 ml of a culture solution prepared by adding citric acid as a growth agent to 10 μg/ml and sodium malate to 100 μg/ml of basic culture seawater. Five alga bodies (leaves cut into pieces of approximately 1 d in size) were placed inside the container, and aerated culture was carried out for 10 days in a constant temperature zone at 15°C under conditions of illuminance of 5500 Lx and 12 hours a day. Ta. Each culture solution was exchanged every two days, the area of the thallus after culture was measured, and the area ratio was determined with the area at the start of culture being 1, and the growth was compared.

The results are shown in Table-6.

Comparative Example 6 200 ml of basic cultured seawater was placed in a 200 ml culture flask, and 5 algae similar to those in Example 10 were placed therein at a constant temperature of 15°C with an illuminance of 5500 Lx.
Aerated culture was carried out for 10 days under the condition of 12 hours of illumination per day. The culture solution was exchanged every two days, and the area of the thallus after culture was measured.The area at the start of culture was taken as 1, and the area ratio was calculated to compare the growth.

Display results 6.

Table 6 18 Example 11 In a petri dish with a diameter of 60III and a depth of 30, 100μ of sodium malate was added to the basic culture and seawater as a growth agent.
30 g/ml of culture solution prepared by adding
ml, and 3 pieces of glass (approximately 1a+Il) with zoospores of Arame attached were placed in this, and cultured for 14 days in a constant temperature zone at 15°C under illuminance of 5500LX and lighting for 12 hours a day. Ta. Each culture solution was exchanged every two days, and the number of cells in the male gametophytes of 30 individuals was counted to determine the average cell number and growth was compared. The results are shown in Table-7.

Comparative Example 7 Put 30 ml of seawater for basic culture in a Petri dish with a diameter of 60 nm + m and a depth of 30 m, and place 3 pieces of glass (approximately 1-) with zoospores attached thereto similar to those in Example 11, 15
Culture was carried out for 14 days in a constant temperature zone at 5500 LX of illuminance and 12 hours of illumination per day. Each culture solution was exchanged every two days, and the number of cells in the male gametophytes of 30 individuals was counted to determine the average number of cells, and each growth was compared.

Display results 7.

Table 7 20 (Effects of the Invention) From the results of Examples and Comparative Examples, the seaweed growth agent composition of the present invention has the effect of promoting the growth of algae and is effective as a seaweed growth agent.

Claims (1)

[Claims] (1) It is characterized by containing at least one organic acid and/or organic acid salt selected from the group consisting of citric acid, isocitric acid, oxaloacetic acid, malic acid, succinic acid, ketoglutaric acid, glutamic acid, and glyoxylic acid. Growth agent for seaweed.