JPH0272874A - イネモミガラパーオキシターゼの製造方法 - Google Patents
イネモミガラパーオキシターゼの製造方法Info
- Publication number
- JPH0272874A JPH0272874A JP22251688A JP22251688A JPH0272874A JP H0272874 A JPH0272874 A JP H0272874A JP 22251688 A JP22251688 A JP 22251688A JP 22251688 A JP22251688 A JP 22251688A JP H0272874 A JPH0272874 A JP H0272874A
- Authority
- JP
- Japan
- Prior art keywords
- peroxidase
- water
- rice hulls
- isopropanol
- crude enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000003992 Peroxidases Human genes 0.000 title claims abstract description 32
- 108040007629 peroxidase activity proteins Proteins 0.000 title claims abstract description 32
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 31
- 235000009566 rice Nutrition 0.000 title claims abstract description 31
- 238000004519 manufacturing process Methods 0.000 title claims description 7
- 240000007594 Oryza sativa Species 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 claims abstract description 41
- 108090000790 Enzymes Proteins 0.000 claims abstract description 41
- 241000209094 Oryza Species 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 18
- 238000005119 centrifugation Methods 0.000 claims abstract description 11
- 239000003960 organic solvent Substances 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims description 4
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 238000000108 ultra-filtration Methods 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 abstract description 5
- 238000000151 deposition Methods 0.000 abstract 1
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 54
- 239000000243 solution Substances 0.000 description 19
- 230000000694 effects Effects 0.000 description 15
- 239000002244 precipitate Substances 0.000 description 14
- 238000000034 method Methods 0.000 description 11
- 238000000605 extraction Methods 0.000 description 8
- 102000004316 Oxidoreductases Human genes 0.000 description 6
- 108090000854 Oxidoreductases Proteins 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000008363 phosphate buffer Substances 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 238000005194 fractionation Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000012510 hollow fiber Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000003365 glass fiber Substances 0.000 description 2
- 239000010903 husk Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- -1 for example Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 150000007530 organic bases Chemical class 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22251688A JPH0272874A (ja) | 1988-09-07 | 1988-09-07 | イネモミガラパーオキシターゼの製造方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22251688A JPH0272874A (ja) | 1988-09-07 | 1988-09-07 | イネモミガラパーオキシターゼの製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0272874A true JPH0272874A (ja) | 1990-03-13 |
| JPH0522510B2 JPH0522510B2 (enrdf_load_stackoverflow) | 1993-03-29 |
Family
ID=16783653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22251688A Granted JPH0272874A (ja) | 1988-09-07 | 1988-09-07 | イネモミガラパーオキシターゼの製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0272874A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5147793A (en) * | 1990-10-18 | 1992-09-15 | The Mead Corporation | Biocatalytic oxidation using soybean peroxidases |
| US5391488A (en) * | 1993-04-19 | 1995-02-21 | Enzymol International, Inc. | Biocatalytic oxidation of a reactive primary alcohol using soybean peroxidase |
| US5491085A (en) * | 1991-05-14 | 1996-02-13 | Enzymol International, Inc. | Method of recovering peroxidase from seed hulls using a freeze-thaw technique |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0724921U (ja) * | 1993-10-04 | 1995-05-12 | 株式会社サンビ | レインウエア |
-
1988
- 1988-09-07 JP JP22251688A patent/JPH0272874A/ja active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5147793A (en) * | 1990-10-18 | 1992-09-15 | The Mead Corporation | Biocatalytic oxidation using soybean peroxidases |
| US5491085A (en) * | 1991-05-14 | 1996-02-13 | Enzymol International, Inc. | Method of recovering peroxidase from seed hulls using a freeze-thaw technique |
| US5391488A (en) * | 1993-04-19 | 1995-02-21 | Enzymol International, Inc. | Biocatalytic oxidation of a reactive primary alcohol using soybean peroxidase |
| US5508180A (en) * | 1993-04-19 | 1996-04-16 | Enzymol International, Inc. | Biocatalytic oxidation using soybean peroxidase |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0522510B2 (enrdf_load_stackoverflow) | 1993-03-29 |
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