JPH0242475B2 - - Google Patents
Info
- Publication number
- JPH0242475B2 JPH0242475B2 JP24549984A JP24549984A JPH0242475B2 JP H0242475 B2 JPH0242475 B2 JP H0242475B2 JP 24549984 A JP24549984 A JP 24549984A JP 24549984 A JP24549984 A JP 24549984A JP H0242475 B2 JPH0242475 B2 JP H0242475B2
- Authority
- JP
- Japan
- Prior art keywords
- microorganisms
- solution
- acrylamide monomer
- aqueous solution
- mixed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 244000005700 microbiome Species 0.000 claims description 33
- 239000000243 solution Substances 0.000 claims description 24
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical group NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 19
- 239000007864 aqueous solution Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 13
- 239000003505 polymerization initiator Substances 0.000 claims description 10
- 235000010413 sodium alginate Nutrition 0.000 claims description 9
- 239000000661 sodium alginate Substances 0.000 claims description 9
- 229940005550 sodium alginate Drugs 0.000 claims description 9
- 239000000725 suspension Substances 0.000 claims description 9
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 8
- 239000001110 calcium chloride Substances 0.000 claims description 8
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 8
- 238000006116 polymerization reaction Methods 0.000 claims description 8
- 230000003100 immobilizing effect Effects 0.000 claims description 4
- 239000002245 particle Substances 0.000 description 15
- 239000007788 liquid Substances 0.000 description 14
- 239000000203 mixture Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 7
- 230000002209 hydrophobic effect Effects 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 4
- 229920002401 polyacrylamide Polymers 0.000 description 4
- 230000000379 polymerizing effect Effects 0.000 description 4
- 230000000241 respiratory effect Effects 0.000 description 4
- MTPJEFOSTIKRSS-UHFFFAOYSA-N 3-(dimethylamino)propanenitrile Chemical compound CN(C)CCC#N MTPJEFOSTIKRSS-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 235000010410 calcium alginate Nutrition 0.000 description 3
- 239000000648 calcium alginate Substances 0.000 description 3
- 229960002681 calcium alginate Drugs 0.000 description 3
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 3
- 239000003431 cross linking reagent Substances 0.000 description 3
- 229910001873 dinitrogen Inorganic materials 0.000 description 3
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000005445 natural material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 241000589565 Flavobacterium Species 0.000 description 1
- 241000589564 Flavobacterium sp. Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- LQEJKDNALLXRCT-UHFFFAOYSA-N chloroform;toluene Chemical compound ClC(Cl)Cl.CC1=CC=CC=C1 LQEJKDNALLXRCT-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- OJURWUUOVGOHJZ-UHFFFAOYSA-N methyl 2-[(2-acetyloxyphenyl)methyl-[2-[(2-acetyloxyphenyl)methyl-(2-methoxy-2-oxoethyl)amino]ethyl]amino]acetate Chemical compound C=1C=CC=C(OC(C)=O)C=1CN(CC(=O)OC)CCN(CC(=O)OC)CC1=CC=CC=C1OC(C)=O OJURWUUOVGOHJZ-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- UBTYFVJZTZYJHZ-UHFFFAOYSA-N n-[2-(prop-2-enoylamino)propyl]prop-2-enamide Chemical compound C=CC(=O)NC(C)CNC(=O)C=C UBTYFVJZTZYJHZ-UHFFFAOYSA-N 0.000 description 1
- CHDKQNHKDMEASZ-UHFFFAOYSA-N n-prop-2-enoylprop-2-enamide Chemical compound C=CC(=O)NC(=O)C=C CHDKQNHKDMEASZ-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- PVGBHEUCHKGFQP-UHFFFAOYSA-N sodium;n-[5-amino-2-(4-aminophenyl)sulfonylphenyl]sulfonylacetamide Chemical compound [Na+].CC(=O)NS(=O)(=O)C1=CC(N)=CC=C1S(=O)(=O)C1=CC=C(N)C=C1 PVGBHEUCHKGFQP-UHFFFAOYSA-N 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Description
【発明の詳細な説明】
産業上の利用分野
本発明は、微生物を活性状態でポリアクリルア
ミドゲルに包括固定する方法に関する。DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention relates to a method for entrapment of microorganisms in an active state in a polyacrylamide gel.
従来の技術
微生物を包括固定にする場合、担体しては、カ
ラギーナン、アルギン酸等の天然に存在する物質
及びポリアクリルアミド等の合成高分子物質が提
案されている。天然物質を担体として使用した場
合には、天然物質は微生物に対する毒性が低いの
で、固定化した微生物の活性低下は少ないが、物
理的強度が小さく、耐久性に問題がある。一方、
ポリアクリルアミドの内部に微生物を固定する場
合、得られる固定微生物の物理的強度は大きく、
耐久性は良いが、固定化する際に使用するアクリ
ルアミドモノマー、架橋剤、重合開始剤等が微生
物に毒性作用を及ぼすので、活性低下を招きやす
い。BACKGROUND TECHNOLOGY When entrapping and immobilizing microorganisms, naturally occurring substances such as carrageenan and alginic acid, and synthetic polymeric substances such as polyacrylamide have been proposed as carriers. When a natural substance is used as a carrier, since the natural substance has low toxicity to microorganisms, the activity of the immobilized microorganisms is less likely to decrease, but the carrier has low physical strength and has problems in durability. on the other hand,
When immobilizing microorganisms inside polyacrylamide, the physical strength of the immobilized microorganisms obtained is large;
Although it has good durability, the acrylamide monomer, crosslinking agent, polymerization initiator, etc. used for immobilization have a toxic effect on microorganisms, which tends to lead to a decrease in activity.
担体としてポリアクリルアミドを使用し、粒状
の固定化微生物を製造する場合、微生物懸濁液を
混合したアクリルアミドモノマー水溶液を撹拌し
た疎水性液体中に粒子状に分散させて重合させる
方法、或いは疎水性液体中に液滴下して重合させ
る方法が行われている。この方法では、疎水性液
体としては、水溶液の分散が均一に安定して行わ
れるトルエン−クロロホルム混合液が一般に用い
られる。 When using polyacrylamide as a carrier to produce granular immobilized microorganisms, a method in which an aqueous acrylamide monomer solution mixed with a microbial suspension is dispersed into particles in a stirred hydrophobic liquid and polymerized, or a hydrophobic liquid A method of polymerizing by dropping droplets into the liquid is used. In this method, a toluene-chloroform mixture is generally used as the hydrophobic liquid, since the aqueous solution can be dispersed uniformly and stably.
発明が解決しようとする問題点
上記の方法で粒状ポリアクリルアミド固定化微
生物を製造する場合には、使用するアクリルアミ
ドモノマー、重合開始剤、架橋剤等の毒性に加え
て、上記のような疎水性液体の毒性により微生物
の活性が大きく低下する場合が多く、また、疎水
性液体や固定化微生物の表面に付着するため、重
合操作の後、これを除去しなければならない欠点
があつた。Problems to be Solved by the Invention When producing granular polyacrylamide-immobilized microorganisms by the above method, in addition to the toxicity of the acrylamide monomer, polymerization initiator, crosslinking agent, etc. used, the hydrophobic liquid In many cases, the activity of microorganisms is greatly reduced due to the toxicity of the polymer, and since it adheres to the surface of hydrophobic liquids and immobilized microorganisms, it has the disadvantage that it must be removed after the polymerization operation.
従つて、本発明は疎水性液体を使用せずに、ア
クリルアミドを粒子状に重合させ、高い活性を有
する固定化微生物を製造しうる方法を提供するこ
とを目的とする。 Therefore, an object of the present invention is to provide a method for producing immobilized microorganisms with high activity by polymerizing acrylamide into particles without using a hydrophobic liquid.
問題点を解決するための手段及び作用
本発明は、微生物を懸濁したアクリルアミドモ
ノマー水溶液に更にアルギン酸ナトリウムを混合
しておき、塩化カルシウムと重合開始剤との混合
水溶液中に滴下して、微生物を包括したアルギン
酸カルシウムの粒子を形成させると同時に、粒子
内部に保持されたアクリルアミドモノマーを重合
させることによつて前記の問題点を解決したもの
である。Means and Effects for Solving the Problems The present invention is directed to the present invention, in which sodium alginate is further mixed with an aqueous acrylamide monomer solution in which microorganisms are suspended, and the mixture is dropped into a mixed aqueous solution of calcium chloride and a polymerization initiator to eliminate microorganisms. The above-mentioned problem is solved by forming particles of calcium alginate that are enclosed in the particles and at the same time polymerizing the acrylamide monomer held inside the particles.
即ち、本発明による微生物の固定方法は、微生
物懸濁液及びアルギン酸ナトリウムを混合したア
クリルアミドモノマー水溶液を混合した後、塩化
カルシウム及び重合開始剤を含む水溶液中に滴下
し、アクリルアミドモノマーを重合させることを
特徴とする。 That is, the method for immobilizing microorganisms according to the present invention involves mixing a microorganism suspension and an aqueous acrylamide monomer solution containing sodium alginate, and then dropping the mixture into an aqueous solution containing calcium chloride and a polymerization initiator to polymerize the acrylamide monomer. Features.
本発明方法では、予め塩化カルシウム及び重合
開始剤を含む水溶液に窒素ガス等の酸素不含ガス
を充分通気して、重合を阻害する溶存酸素をなく
し、嫌気性雰囲気下に操作することにより、滴下
した粒子内部に保持されたアクリルアミドモノマ
ーを短時間で確実に重合させることができる。 In the method of the present invention, an aqueous solution containing calcium chloride and a polymerization initiator is thoroughly aerated with an oxygen-free gas such as nitrogen gas in advance to eliminate dissolved oxygen that inhibits polymerization, and the solution is operated under an anaerobic atmosphere. The acrylamide monomer held inside the particles can be reliably polymerized in a short time.
本発明方法において、モノマー水溶液には、通
常、架橋剤としてＮ，N′−メチレンビスアクリ
ルアミド、Ｎ，N′−プロピレンビスアクリルア
ミド、ジアクリルアミドジメチルエステル等を溶
解し、更に必要に応じて重合促進剤、例えばβ−
ジメチルアミノプロピオニトリル、Ｎ，Ｎ，N′，
N′−テトラメチルエチレンジアミン等を溶解し
ておく。 In the method of the present invention, N,N'-methylenebisacrylamide, N,N'-propylenebisacrylamide, diacrylamide dimethyl ester, etc. are usually dissolved in the monomer aqueous solution as a crosslinking agent, and if necessary, a polymerization accelerator is also added. , for example β-
Dimethylaminopropionitrile, N, N, N',
Dissolve N'-tetramethylethylenediamine, etc.
重合開始剤としては、通常、ベルオクソ二硫酸
カリウムを使用する。 As a polymerization initiator, potassium beroxodisulfate is usually used.
次に、図面に基づいて本発明を説明する。 Next, the present invention will be explained based on the drawings.
図面は、本発明方法を実施する装置の一実施態
様を示す。図面に示した装置においては、まず、
ラインミキサー１とポンプ２及びポンプ３を用い
て、微生物懸濁液４とアルギン酸ナトリウム水溶
液５及びアクリルアミドモノマー水溶液６を混合
する。更に、この混合液をラインミキサー１に接
続したノズル７から塩化カルシウム・重合開始剤
水溶液８中に滴下すると、アルギン酸カルシウム
の粒子が形成すると同時に粒子内部でアクリルア
ミドモノマーの重合が開始し、３〜15分程度て粒
子状の固定化微生物９が完成する。粒径は、滴下
する混合液の粘度、ノズル７の口径等の調節によ
り変えることができ、１〜８mmのものが得られ
る。ノズル７の部分には、窒素ガス等の酸素不含
ガス１０を通気し、嫌気性雰囲気にすることがで
きる。塩化カルシウム・重合開始剤水溶液８は、
重合時の発熱による温度上昇を防ぐ必要があり、
ウオータージヤケツト１１を用いて20〜35℃で保
たれる。 The drawing shows an embodiment of a device for carrying out the method of the invention. In the device shown in the drawing, first,
Using line mixer 1, pump 2, and pump 3, microorganism suspension 4, sodium alginate aqueous solution 5, and acrylamide monomer aqueous solution 6 are mixed. Furthermore, when this mixed solution is dropped into the calcium chloride/polymerization initiator aqueous solution 8 from the nozzle 7 connected to the line mixer 1, the acrylamide monomer starts polymerizing inside the particles at the same time as calcium alginate particles are formed. Particulate immobilized microorganisms 9 are completed in about a minute. The particle size can be changed by adjusting the viscosity of the liquid mixture to be dropped, the diameter of the nozzle 7, etc., and a particle size of 1 to 8 mm can be obtained. An oxygen-free gas 10 such as nitrogen gas can be passed through the nozzle 7 to create an anaerobic atmosphere. Calcium chloride/polymerization initiator aqueous solution 8 is
It is necessary to prevent temperature rise due to heat generation during polymerization.
It is maintained at 20-35°C using a water jacket 11.
ラインミキサー１内で混合するアルギン酸ナト
リウム及びアクリルアミドモノマーの濃度は、適
宜変化させることができ、通常、混合液中でアル
ギン酸ナトリウム濃度が0.1〜２％、アクリルア
ミドモノマー濃度が５〜30％になるように混合す
る。 The concentrations of sodium alginate and acrylamide monomer mixed in the line mixer 1 can be changed as appropriate, and usually the concentration of sodium alginate is 0.1 to 2% and the concentration of acrylamide monomer is 5 to 30% in the mixed liquid. Mix.
実施例
次に、実施例に基づいて本発明を詳述するが、
本発明はこれに限定されるものではない。Examples Next, the present invention will be described in detail based on examples.
The present invention is not limited to this.
実施例 １ まず、下記の組成のＡ〜Ｄ液を調製した。Example 1 First, solutions A to D having the following compositions were prepared.
Ａ液：シユードモナス属菌（Pseudomonas sp.）
6.5×107細胞／cm3懸濁液
Ｂ液：1.5％アルギン酸ナトリウム水溶液
Ｃ液：アクリルアミドモノマー54％、Ｎ，N′−
メチレンビスアクリルアミド３％、β−ジメチ
ルアミノプロピオニトリル1.5％の混合水溶液
Ｄ液：塩化カルシウム１％、ペルオクソ二硫酸カ
リウム0.5％の混合水溶液
図面に示した装置を用いて、Ａ液、Ｂ液及びＣ
液を等量ずつ混合し、予め窒素ガスを充分に通気
したＤ液中に滴下した。ノズルの口径は1.5mmと
した。アルギン酸カルシウムが生成し、アクリル
アミドの重合が開始した。Ｄ液の温度を30℃に保
持し、粒子が互いに付着するのを防ぐため、Ｄ液
を緩やかに撹拌し続けた。数分で重合が終了し、
固定化微生物が得られた。Solution A: Pseudomonas sp.
6.5×10 7 cells/cm 3 Suspension B solution: 1.5% sodium alginate aqueous solution C solution: Acrylamide monomer 54%, N,N'-
A mixed aqueous solution of 3% methylenebisacrylamide and 1.5% β-dimethylaminopropionitrile Solution D: A mixed aqueous solution of 1% calcium chloride and 0.5% potassium peroxodisulfate. C
The liquids were mixed in equal amounts and dropped into liquid D, which had been sufficiently aerated with nitrogen gas in advance. The diameter of the nozzle was 1.5 mm. Calcium alginate was produced and acrylamide polymerization started. The temperature of Solution D was maintained at 30° C., and Solution D was continued to be gently stirred to prevent particles from adhering to each other. Polymerization is completed in a few minutes,
Immobilized microorganisms were obtained.
固定微生物の粒径は３〜４mmであり、呼吸活性
はフリーの微生物の35％であつた。 The particle size of the immobilized microorganisms was 3-4 mm, and the respiratory activity was 35% of that of the free microorganisms.
実施例 ２ まず、下記の組成のＡ〜Ｄ液を調製した。Example 2 First, solutions A to D having the following compositions were prepared.
Ａ液：フラボバクテリウム属菌
（Flavobacterium sp.）2.3×107細胞／cm3懸濁
液
Ｂ液：３％アルギン酸ナトリウム水溶液
Ｃ液及びＤ液：実施例１に同じ
実施例１と同様な方法で固定化微生物を製造し
た。粒径は４〜５mmであり、呼吸活性はフリーの
微生物の78％であつた。Solution A: Flavobacterium sp. 2.3×10 7 cells/cm 3 suspension Solution B: 3% aqueous sodium alginate solution Solutions C and D: Same as Example 1 Same method as Example 1 Immobilized microorganisms were produced. The particle size was 4-5 mm and the respiratory activity was 78% of free microorganisms.
比較例 １ 下記の組成のＡ〜Ｃ液を調製した。Comparative example 1 Solutions A to C having the following compositions were prepared.
Ａ液：シユードモナス属菌4.3×108細胞／cm3懸濁
液
Ｂ液：アクリルアミドモノマー36％、Ｎ，N′−
メチレンビスアクリルアミド２％、β−ジメチ
ルアミノプロピオニトリル１％の混合水溶液
Ｃ液：トルエン（73％Ｖ／Ｖ）＋クロロホルム
（27％Ｖ／Ｖ）
Ａ液とＢ液を等量ずつ混合し、口径1.5mmのノ
ズルを用いて、緩やかに撹拌したＣ液中に滴下し
た。Ｃ液の温度は30℃に保持した。数分で重合が
終了し、粒径３〜４mmの固定化微生物が得られ
た。呼吸活性はフリーの微生物の５％であつた。Solution A: Pseudomonas 4.3 x 10 8 cells/cm 3 suspension Solution B: 36% acrylamide monomer, N,N'-
Mixed aqueous solution of 2% methylenebisacrylamide and 1% β-dimethylaminopropionitrile Solution C: Toluene (73% V/V) + Chloroform (27% V/V) Mix equal amounts of Solutions A and B, Using a nozzle with a diameter of 1.5 mm, it was dropped into the gently stirred liquid C. The temperature of liquid C was maintained at 30°C. Polymerization was completed in a few minutes, and immobilized microorganisms with a particle size of 3 to 4 mm were obtained. Respiratory activity was 5% of free microorganisms.
比較例 ２ 下記の組成のＡ〜Ｃ液を調製した。Comparative example 2 Solutions A to C having the following compositions were prepared.
Ａ液：フラボバクテリウム属菌1.5×107細胞／cm3
懸濁液
Ｂ液及びＣ液：比較例１に同じ
比較例１と同様な方法で固定化微生物を製造し
た。粒径は４〜５mmであり、呼吸活性はフリーの
微生物の11％であつた。Solution A: Flavobacterium 1.5 x 107 cells/ cm3
Suspensions B and C: Same as Comparative Example 1 Immobilized microorganisms were produced in the same manner as in Comparative Example 1. The particle size was 4-5 mm and the respiratory activity was 11% of free microorganisms.
発明の効果
本発明によれば、疎水性液体を使用することな
く粒状の固定化微生物を得ることができ、その固
定化微生物は従来法で製造されるものに比べて著
しく高い活性を保有する。Effects of the Invention According to the present invention, granular immobilized microorganisms can be obtained without using a hydrophobic liquid, and the immobilized microorganisms have significantly higher activity than those produced by conventional methods.
図面は本発明方法を実施する装置の一実施態様
を示す略示系統図である。
１……ラインミキサー、４……微生物懸濁液、
５……アルギン酸ナトリウム水溶液、６……アク
リルアミドモノマー水溶液、８……塩化カルシウ
ム・重合開始剤水溶液。
The drawing is a schematic system diagram showing one embodiment of an apparatus for carrying out the method of the invention. 1... line mixer, 4... microorganism suspension,
5... Sodium alginate aqueous solution, 6... Acrylamide monomer aqueous solution, 8... Calcium chloride/polymerization initiator aqueous solution.
Claims (1)
合したアクリルアミドモノマー水溶液を混合した
後、塩化カルシウム及び重合開始剤を含む水溶液
中に滴下し、アクリルアミドモノマーを重合させ
ることを特徴とする微生物の固定方法。 ２ 滴下及び重合操作を嫌気性雰囲気下に行う特
許請求の範囲第１項記載の微生物の固定方法。[Scope of Claims] 1. A microorganism characterized in that a microorganism suspension and an aqueous acrylamide monomer solution containing sodium alginate are mixed and then dropped into an aqueous solution containing calcium chloride and a polymerization initiator to polymerize the acrylamide monomer. fixing method. 2. The method for immobilizing microorganisms according to claim 1, wherein the dropping and polymerization operations are performed in an anaerobic atmosphere.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24549984A JPS61124385A (en) | 1984-11-20 | 1984-11-20 | Immobilization of microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24549984A JPS61124385A (en) | 1984-11-20 | 1984-11-20 | Immobilization of microorganism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61124385A JPS61124385A (en) | 1986-06-12 |
| JPH0242475B2 true JPH0242475B2 (en) | 1990-09-21 |
Family
ID=17134572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24549984A Granted JPS61124385A (en) | 1984-11-20 | 1984-11-20 | Immobilization of microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61124385A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04154192A (en) * | 1990-10-17 | 1992-05-27 | Fujitsu Ltd | Plating of printed wiring board |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5331066A (en) * | 1988-06-24 | 1994-07-19 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Process for producing polyester ether copolymer |
-
1984
- 1984-11-20 JP JP24549984A patent/JPS61124385A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04154192A (en) * | 1990-10-17 | 1992-05-27 | Fujitsu Ltd | Plating of printed wiring board |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61124385A (en) | 1986-06-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0048109B1 (en) | Improvements in or relating to composite materials | |
| CN107746841B (en) | Zwitterion magnetic composite hydrogel immobilized enzyme carrier and preparation method thereof | |
| US4194066A (en) | Immobilization of enzymes or bacteria cells | |
| US4927761A (en) | Immobilization of cells with alginate and agarose | |
| JPH0242475B2 (en) | ||
| CN112980826A (en) | Lipase/polyacrylamide hydrogel microsphere catalytic material and preparation method and application thereof | |
| JP3361570B2 (en) | Microorganism immobilization method using polymer hydrogel granules | |
| JPS649073B2 (en) | ||
| Nilsson et al. | [19] Entrapment of microbial and plant cells in beaded polymers | |
| JPH0827214A (en) | Production of hydrous gel, heavy metallic ion adsorbent, coloring matter adsorbent, microbial carrier and carrier for immobilizing enzyme | |
| JPH0417631B2 (en) | ||
| JP3265981B2 (en) | Entrapping immobilization carrier for nitrogen removal and method for forming the same | |
| JP4384338B2 (en) | Enzyme or microorganism-immobilized carrier composition | |
| JPS6219083A (en) | Immobilization of microorganism | |
| JP3687292B2 (en) | Method for entrapping immobilization of nitrifying bacteria and method for producing entrapping immobilization support | |
| JPH0716585A (en) | Water-containing granulated carrier for organism treatment apparatus and method for manufacturing the carrier | |
| JPH0528107B2 (en) | ||
| Mertens et al. | HLAD immobilization in polyacrylamide gels | |
| JPH0745593B2 (en) | Reinforcing method of gel polymer | |
| JPH0135637B2 (en) | ||
| JPS63276488A (en) | Method for immobilizing microbial cell | |
| JPH0265782A (en) | Production of microorganism-immobilized granular gel | |
| JPH01137975A (en) | Immobilization of microorganism, enzyme and chemical substance | |
| JPH0427837B2 (en) | ||
| JPH0327197B2 (en) |