JPH02287261A - Automatic analyser - Google Patents

Abstract

PURPOSE:To measure a specimen under a plurality of measuring conditions using reagents set at the same position by providing a part inputting the reagent position corresponding to each of the first reagent the n-th reagent at every measuring item to store the same. CONSTITUTION:A specimen to be measured is received in specimen cups 8 to be arranged on a specimen disc 9 and, when each of the cups 8 reaches a predetermined specimen collecting position, a definite amount of the specimen is sucked by a specimen pipetter 12 to be emitted to a reaction container 11. A reagent is added to the container 11 by a reagent pipetter 12 to advance chemical reaction. The container 11 is immersed in the water always received in a reaction tack 19 to be subjected to temp. control and optical measurement is also performed by the direct photometry of the reaction container through the water subjected to temp. control. The light emitted from a light source is transmitted through the specimen solution to be examined in the container 11 to be spectrally diffracted by a grating 21 and measured as absorbancy of a specific wavelength by a detector 22 to be converted to a digital signal which is, in turn, converted to concn. by a CPU 3 to be outputted.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to an automatic analyzer, and more particularly, to an automatic analyzer that can perform measurements under different analysis conditions using reagents set at the same position. The automatic analyzer of the present invention can be applied to samples other than clinical analysis samples, and its range of use is wide.

[Conventional technology]

In the conventional apparatus, only one analysis condition can be set for a reagent set in one reagent position as described in Japanese Utility Model Application Publication No. 56-31358. Utility Model Application No. 56-31358 identifies a reagent holder, reads out the analysis conditions corresponding to that reagent holder, and performs automatic analysis of multiple desired analysis items.
(reagent holder) and analysis conditions correspond to 1=1. Also, even with Hitachi's conventional equipment, there is only one analysis condition corresponding to the channel in which the reagent bottle is set (two only in special cases).
Therefore, it was not possible to perform measurements simultaneously under multiple different analysis conditions using reagents set at the same position. In order to simultaneously measure multiple items with different analysis conditions using the same reagent using conventional equipment, it is necessary to divide the reagent into multiple bottles and set the analysis conditions for each bottle before measuring. However, in this case, it may take time to prepare the reagents and open the bottle. Problems arise, such as the dead volume of each bottle, which requires extra reagents, which is uneconomical, and the limited number of channels in which reagent bottles can be set, which reduces the number of items that can be set at the same time. On the other hand, it is not possible to simultaneously measure multiple items under different analysis conditions using reagents set in the same position, so if you stick to the same position, you will have to change the analysis conditions each time you finish measuring under one analysis condition. This resulted in a huge loss of 7 hours, and in any case, there was a big problem in practical use.

[Problem to be solved by the invention]

The above-mentioned conventional technology does not take into consideration the possibility of performing measurements under a plurality of different measurement conditions using reagents set at the same position.

In recent years, there has been an increase in requests for urine as a target sample to be analyzed using automatic analyzers, and serum and urine are often measured using the same device, and the concentration of components in urine is generally several times to several times the concentration of components in serum. 100 times, and significantly different concentration ranges. Therefore, in order to measure the same item with the same reagent, either pre-dilute the sample or set the same reagent in small portions according to the analysis conditions.

It was necessary to set analysis conditions according to each specimen type before performing measurements.

Also, when considering the optimal analysis conditions for a reagent with an automatic analyzer, only one analysis condition can be set for one reagent, and when measuring the same reagent under multiple analysis conditions at the same time. , reagents had to be divided into small portions for the number of analytical conditions and then measured.

Furthermore, there was no consideration given to conducting basic experiments such as determining the Michaelis constant (Km) of enzymatic reactions. The reagents must be prepared in multiple reagent bottles and measured under different analysis conditions, or one of the methods must be used, which is the ``conventional technique'' described above.
There was a problem as mentioned above.

An object of the present invention is to solve the above problems and provide an automatic analyzer that can perform measurements under a plurality of different measurement conditions using reagents set at the same position.

[Means to solve the problem]

In order to achieve the above purpose, the automatic analyzer is equipped with a section for inputting and storing the reagent positions corresponding to the first reagent...nth reagent for each measurement item, and stores the stored input information and requested items. By comparing names, it is possible to define reagents set in the same reagent position for multiple analysis items.

[Effect]

By providing a section for inputting and storing reagent positions corresponding to the first reagent to the nth reagent for each measurement item, the reagent position measurement items can correspond one to many. Thereby, measurements can be performed under a plurality of different measurement conditions using reagents set at the same position.

〔Example〕

An embodiment of the present invention will be described below with reference to FIG. 1st
The figure shows the overall configuration of an automatic analyzer based on the present invention.

The automatic analyzer has main mechanical components housed in a main body 1, and each mechanical component is controlled by a central control unit (CPU) 3 via an interface 2. When trying to analyze a sample 1. The analysis conditions for each sample are as follows:
The results measured according to the zero analysis conditions inputted from the keyboard 4 and stored in the magnetic storage section (floppy disk) 5 are displayed on the display device (CRT) 6 and printed out by the printer 7 for reporting the results.

Samples to be measured are placed in a sample cup 8 and placed side by side on a sample disk 9. When the sample cup reaches a predetermined sample collection position, a certain amount of sample is aspirated by the sample pipetter 10 and discharged into the reaction container 11 . A reagent is added to the reaction container by a reagent pipetter 12, and a chemical reaction proceeds. Reagents are stored in a reagent bottle 13 and arranged on a reagent disk 14 via a reagent holder 15. The inside of the reagent holder is hollow, and the reagent reaches the lower part of the reagent nozzle 16 through the cavity. The reagent pipettor has a reagent nozzle installed at its tip, then aspirates a certain amount of reagent.
Discharge into reaction vessel. The reaction vessels are arranged around the outer periphery of the reaction disk 17, and the reaction progresses by one reaction vessel at a given time.
During this time, sample addition, reagent addition, and stirring by the mixer 18 are performed.

The reaction vessel is always immersed in temperature-controlled water filled in the reaction tank 19, and optical measurements are also performed by direct photometry of the reaction vessel through the temperature-controlled water. After the light emitted from the light source passes through the test sample liquid in the reaction container, it is separated into spectra by the grating 21, measured as absorbance at a specific wavelength by the detector 22, converted into a digital signal, and converted into concentration by the CPU. It will be output later.

The present invention measures the above-mentioned different analysis items one after another (adding the ability to the conventional automatic analyzer to perform measurements under a plurality of different measurement conditions using reagents set at the same position). be. FIG. 2 is a flowchart for measuring a plurality of items under different analysis conditions using the same reagent when the present invention is implemented using an automatic analyzer. A column is provided to set the reagent position as one of the analysis conditions, and in addition to the conventional sample pipetting amount, photometric wavelength, data calculation method, etc., it is necessary to input the reagent pipetting amount and reagent position. In the case of a two-reagent system,
It is necessary to input the reagent pipetting amount and reagent position for each of the first reagent and the second reagent, and for each reagent in the case of an n-reagent system. When an analysis item is requested and the analysis is started, the sample is aspirated and discharged according to the above analysis conditions, and the first reagent, second reagent, etc.
A specified amount of reagent is aspirated and discharged from a specified reagent position, and analysis is performed as described above, so it is possible to measure multiple items with different analysis conditions using the same reagent. Furthermore, if necessary, it is also possible to print the remaining amount of reagent according to the amount of reagent for the analysis conditions of each measurement item. This determines whether multiple items are to be analyzed at a designated reagent position, and if multiple items are to be measured, the remaining amount of reagent is calculated and output according to the analysis conditions for each item.

According to this embodiment, when measuring samples with significantly different concentration ranges of components, such as serum and urine, it is possible to register serum and Fukagawa as separate items in advance and set the analysis conditions for each. , it becomes possible to perform measurements under two different analytical conditions using the same reagent. Furthermore, when examining optimal analysis conditions for reagents using an automatic analyzer, it becomes possible to set a plurality of analysis conditions for the same reagent in the same bottle. This eliminates the need to divide prepared reagents into reagent bottles, which was conventionally required for the above-mentioned measurements, leading to labor savings, as well as eliminating the possibility of opening the bottle, ensuring accurate measurements. Now I can do it. Furthermore, you can save on reagents,
The number of items that can be analyzed simultaneously has also increased.

Honzuke can also be applied to basic experiments on enzyme reactions.

The Michaelis constant Km value of an enzymatic reaction can be determined by measuring the reaction rate at a substrate concentration. For example, in a two-reagent system, the first reagent is a buffer, the second
When the reagent is a substrate solution (dissolved in the buffer of the first reagent),
As shown in Table 1, all analysis conditions other than the first reagent pipetting amount and the second reagent pipetting amount are set equal, and the pipetting amount of the second reagent (substrate solution) is adjusted in stages, and the pipetting amount of the first reagent is Enter the amounts so that the total amount of reagents is equal. For a certain sample, the Km value is automatically calculated and output by the least squares method from the absorbance (or absorbance change rate) of items 1 to 6 and the substrate amount under the analysis conditions. FIG. 3 shows the results of an experiment to determine the Km value of amylase when paranitrophenyl α.D-gelcondo was used as a substrate. In Figure 3, the horizontal axis shows the substrate concentration, and the vertical axis shows the substrate concentration/initial velocity, and the K
The m value is the output Km value 1. It was consistent with 1X10-3M.

〔Effect of the invention〕

According to the present invention, since it is possible to measure a plurality of items under different analysis conditions using reagents set at the same position, it is possible to easily measure a plurality of samples containing different concentrations of the analyte. In addition, studies on optimal conditions for reagents and basic experiments on enzyme reactions can be performed quickly, efficiently, accurately, and economically. Furthermore, since multiple items can be measured using reagents in one position, the number of items that can be analyzed simultaneously has expanded more than ever before.

[Brief explanation of drawings]

Fig. 1 is an overall configuration diagram of an embodiment of the present invention, Fig. 2 is a processing flowchart 1-1 for each analysis when performing measurements according to an embodiment, and Fig. 3 is a Michaelis diagram of an enzyme reaction in an embodiment. It is a diagram for finding constants. 1...Device body, 2...Interface, 3...
CPU, 4...keyboard, 5...storage device, 6...
・Display section, 7... Printer, 8... Sample container, 9.
...Sample disk, 10...Sample pipettor, 11...
- Reaction container, 12... Reagent pipetter, 13... Reagent bottle, 14... Reagent disk, 15... Reagent holder, 16... Reagent chip, 17... Reaction disk, 1
8...Mixer, 19...Tsubaki constant temperature, 20...Light source,
21... Spectrometer, 22... Detector. Moku 2-ku Ichireisan Nora (TrLM, )

Claims (1)

[Claims] 1. An automatic analysis device having a reagent position in which one or more reagents can be set, characterized in that measurements can be performed under a plurality of different measurement conditions using reagents set in the same position. Device. 2. The automatic analyzer according to claim 1, which is capable of measuring a plurality of samples containing different concentrations of the target to be analyzed under optimal conditions according to the concentrations. 3. The automatic analyzer according to claim 1, which is capable of measuring the same sample under a plurality of different measurement conditions using the same reagent.