JPH0226320Y2 - - Google Patents
Info
- Publication number
- JPH0226320Y2 JPH0226320Y2 JP14626785U JP14626785U JPH0226320Y2 JP H0226320 Y2 JPH0226320 Y2 JP H0226320Y2 JP 14626785 U JP14626785 U JP 14626785U JP 14626785 U JP14626785 U JP 14626785U JP H0226320 Y2 JPH0226320 Y2 JP H0226320Y2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- microorganisms
- culture solution
- cultured
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 244000005700 microbiome Species 0.000 claims description 24
- 241001465754 Metazoa Species 0.000 claims description 9
- 239000006260 foam Substances 0.000 claims description 8
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000003756 stirring Methods 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 238000009423 ventilation Methods 0.000 description 5
- 239000000969 carrier Substances 0.000 description 4
- 125000005442 diisocyanate group Chemical group 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 229930182555 Penicillin Natural products 0.000 description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 229940049954 penicillin Drugs 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- 239000012533 medium component Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 229920001451 polypropylene glycol Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- -1 glycerin and alcohol Natural products 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 229930010796 primary metabolite Natural products 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【考案の詳細な説明】
〔産業上の利用分野〕
本考案は、微生物・動植物培養細胞等を液体培
地内にて培養する装置に関し、特に培養液を攬拌
させて微生物等の増殖を促進させ、微生物等を大
量に採取し得る攬拌型の培養装置に関する。[Detailed description of the invention] [Industrial field of application] The present invention relates to a device for culturing microorganisms, cultured animal and plant cells, etc. in a liquid medium, and in particular, a device for culturing microorganisms, cultured animal and plant cells, etc. , relates to an agitation-type culturing device capable of collecting large amounts of microorganisms, etc.
液体培地内で、かび・放線菌等の微生物・動植
物培養細胞を培養すると、微生物等は増殖し、グ
リセリン・アルコール等の一次代謝物、あるいは
ペニシリン・ストレプトマイシン等の二次代謝物
が生産される。
When microorganisms such as molds and actinomycetes, as well as animal and plant culture cells, are cultured in a liquid medium, the microorganisms proliferate and produce primary metabolites such as glycerin and alcohol, or secondary metabolites such as penicillin and streptomycin.
一般に、微生物が混入された液体培地を攬拌す
ると、微生物等は倍地成分との接触および酸素の
供給がよくなり、増殖が均一となるばかりか、増
殖効率も上昇する。培養される微生物がかびの場
合には、培養液の攬拌が充分であれば菌糸が分散
し、パルプ状の成長（pulpy growth）を示すが、
攬拌が不充分なときは小球状の菌糸塊（pellet）
になる。前者の場合には、菌糸が培養液内に分
散・懸濁するため、培養液の粘性が増加し、その
攬拌のために多大な動力を要するという欠点があ
る。後者の場合には、菌糸塊の内部にまで酸素・
培地成分が充分に供給されないために、増殖が充
分に行われず、例えばペニシリン生産菌を培養す
る場合には、ペニシリンの生産速度が低下してそ
の収量が著しく減少するという欠点があつた。 Generally, when a liquid medium mixed with microorganisms is stirred, the microorganisms etc. come into better contact with the medium components and are supplied with oxygen, which not only results in uniform growth but also increases the growth efficiency. When the microorganism to be cultured is mold, if the culture solution is sufficiently agitated, the hyphae will disperse and show pulpy growth.
If stirring is insufficient, small spherical mycelial masses (pellets)
become. In the former case, the hyphae are dispersed and suspended in the culture solution, which increases the viscosity of the culture solution and requires a large amount of power to stir it. In the latter case, oxygen and
Since sufficient medium components are not supplied, sufficient growth is not achieved. For example, when culturing penicillin-producing bacteria, the production rate of penicillin decreases, resulting in a significant decrease in yield.
このような問題点を解決するために、本願考案
者らが、培養液中に分散された複数の発泡担体内
でかび等の微生物・動植物培養細胞を培養する方
法（特願昭59−71515号）を開発した。この培養
方法は、例えば、ポリプロピレングリコールジイ
ソシアネートとポリエチレングリコールジイソシ
アネートから連続発泡法で製造した多孔質のウレ
タンフオームを発泡担体として、培養される微生
物等が混入された培養液内に分散させて微生物等
を培養するものであり、微生物等は発泡担体に付
着して発泡担体における細孔内で増殖する。この
培養方法によれば、微生物等は、酸素・培地成分
との接触が良くなるために、その増殖が促進さ
れ、また菌糸が培養液中に分散懸濁しないために
攬拌が円滑に行なえる。 In order to solve these problems, the inventors of the present application have developed a method of culturing cultured cells of microorganisms such as mold, animals and plants in a plurality of foamed carriers dispersed in a culture solution (Japanese Patent Application No. 59-71515). ) was developed. In this culture method, for example, a porous urethane foam produced by a continuous foaming method from polypropylene glycol diisocyanate and polyethylene glycol diisocyanate is used as a foam carrier, and the microorganisms, etc. to be cultured are dispersed in a culture solution mixed with the microorganisms. Microorganisms and the like adhere to the foamed carrier and proliferate within the pores of the foamed carrier. According to this culture method, microorganisms etc. have better contact with oxygen and culture medium components, so their growth is promoted, and since mycelia are not dispersed and suspended in the culture solution, stirring can be performed smoothly. .
この方法を実施する装置としては、培養槽内
に、発泡担体を混入した培養液を投入し、培養槽
の下側より空気等を送給して培養液を攬拌流動さ
せる流動層型の培養装置が用いられ得る。しか
し、この流動層型の培養装置は、培養液を空気等
により攬拌させなければならず、特にかび等の好
気性微生物を培養する場合には、送給すべき空気
量を非常に多くする必要があるため、経剤性が損
なわれる。これに対し、効率よく通気・攬拌が行
え得るという点で、培養液中に設けられた羽根車
を回転させて培養液を攬拌させる回転翼式の培養
装置は非常に有利である。しかし、羽根車の回転
により培養液中の発泡担体がせん断・破損される
という欠点があつた。 A device for carrying out this method is a fluidized bed type culture in which a culture solution mixed with a foamed carrier is placed in a culture tank, and air, etc. is supplied from the bottom of the culture tank to agitate and fluidize the culture solution. A device can be used. However, in this fluidized bed type culture device, the culture solution must be stirred with air, etc., and the amount of air that must be supplied is extremely large, especially when culturing aerobic microorganisms such as mold. Because it is necessary, the medicinal properties are impaired. On the other hand, a rotary vane type culture device that rotates an impeller provided in the culture solution to agitate the culture solution is very advantageous in that it can perform aeration and stirring efficiently. However, there was a drawback that the foamed carrier in the culture solution was sheared and damaged due to the rotation of the impeller.
本考案は上記従来技術の問題点を解決するため
のものであり、その目的は、例えば培養液中に分
散された発泡担体を損傷するおそれがなく、効率
よく微生物等を増殖させ得る培養装置を提供する
ことにある。
The present invention is intended to solve the above-mentioned problems of the prior art, and its purpose is to provide a culture device that can efficiently propagate microorganisms, etc. without the risk of damaging the foamed carriers dispersed in the culture solution. It is about providing.
本考案の培養装置は、培養すべき微生物、動植
物培養細胞等が混入された培養液を培養槽内に投
入し、回転体により攬拌して微生物、動植物培養
細胞等を増殖させる培養装置であつて、前記回転
体は、培養槽内の底面部に近接し、その上面に上
方に突出する突部が設けられた回転板を具備して
なり、そのことにより上記目的が達成される。
The culture device of the present invention is a culture device in which a culture solution mixed with microorganisms, cultured animal and plant cells, etc. to be cultured is put into a culture tank, and is agitated by a rotating body to proliferate microorganisms, cultured animal and plant cells, etc. The rotating body is provided with a rotating plate that is close to the bottom of the culture tank and has a protrusion projecting upwardly on the top surface of the rotating plate, thereby achieving the above object.
以下に本考案の実施例について説明する。 Examples of the present invention will be described below.
本考案の培養装置は、第１図に示すように、蓋
部１１を有する培養槽１０と、該培養槽１０内に
投入された培養液４１を攬拌するための回転体２
０とを具備する。 As shown in FIG. 1, the culture apparatus of the present invention includes a culture tank 10 having a lid part 11, and a rotating body 2 for stirring the culture solution 41 put into the culture tank 10.
0.
培養槽１０の底部には、該培養槽１０の内部と
外部とを連通する通気管３０が配設されている。
該通気管３０の培養槽１０内に位置する部分に
は、小孔３１，３１，…が形成されており各小孔
３１より培養液中に空気が供給される。 A ventilation pipe 30 is provided at the bottom of the culture tank 10 to communicate the inside and outside of the culture tank 10.
Small holes 31, 31, . . . are formed in the portion of the ventilation pipe 30 located inside the culture tank 10, and air is supplied into the culture solution from each small hole 31.
培養槽１０の底面部１２の上面中央部には、凹
状に切欠された凹部１２１が形成されており、該
凹部１２１に回転体２０における回転板２１ぎ嵌
合してい。回転板２１は円板状をしており、その
上面中央部には回転軸２４の一端が連結されてい
る。該回転軸２４の他端は蓋部１１を貫通し、モ
ーター等の適当な回転駆動手段に連結されてい
る。 A concave portion 121 is formed in the center of the upper surface of the bottom portion 12 of the culture tank 10, and the rotary plate 21 of the rotating body 20 is fitted into the concave portion 121. The rotating plate 21 has a disk shape, and one end of a rotating shaft 24 is connected to the center of the upper surface thereof. The other end of the rotating shaft 24 passes through the lid 11 and is connected to a suitable rotational drive means such as a motor.
回転板２１の上面には、底面部１２の上面より
は若干上方に突出し、第２図に示すように、それ
ぞれが回転板２１の中央部より径方向に延びる４
つの突部２２，２２，…が４等配に設けられてい
る。 On the upper surface of the rotary plate 21, there are 4 grooves projecting slightly upward from the upper surface of the bottom part 12, and each extending in the radial direction from the center of the rotary plate 21, as shown in FIG.
Four protrusions 22, 22, . . . are provided at four equal intervals.
上記実施例では培養槽の底面に凹部を設けた
が、本考案においては培養槽の底面を平面とし、
この底面との間に小間隙を有するように回転板を
設けることもできる。 In the above embodiment, a recess was provided on the bottom of the culture tank, but in the present invention, the bottom of the culture tank is flat,
A rotary plate can also be provided with a small gap between it and the bottom surface.
斯かる構成の培養装置は液体培地中に増殖させ
るべき微生物・動植物培養細胞等を添加した培養
液４１を培養槽１０内に投入し、その培養液４１
中に、例えばポリビニルアルコールを発泡させた
もの、あるいはポリエチレングリコールジイソシ
アネートとポリプロピレングリコールジイソシア
ネートから連続発泡法で製造したウレタンフオー
ム等の発泡担体４２を分散させる。このような状
態で、通気管３０の各小孔３１から空気を通流さ
せると共に、回転体２０の回転板２１を回転させ
る。回転板２１が回転すると、その上面に設けら
れた各突部２２により、培養液４１は攬拌され
る。培養液４１内の微生物等は、発泡担体４２の
細孔に入り込んで増殖するが、培養液４１が攬拌
されることにより、液体培地と各発泡担体４２と
はよく接触し、かつ通気管３０より送給される空
気が発泡担体４２とよく接触して増殖が促進され
る。
In the culture device having such a configuration, a culture solution 41 to which microorganisms, cultured animal and plant cells, etc. to be grown are added to a liquid medium is put into the culture tank 10, and the culture solution 41 is added to the culture tank 10.
A foamed carrier 42 such as foamed polyvinyl alcohol or urethane foam produced from polyethylene glycol diisocyanate and polypropylene glycol diisocyanate by a continuous foaming method is dispersed therein. In this state, air is allowed to flow through each small hole 31 of the ventilation pipe 30, and the rotating plate 21 of the rotating body 20 is rotated. When the rotary plate 21 rotates, the culture solution 41 is stirred by each protrusion 22 provided on its upper surface. Microorganisms and the like in the culture solution 41 enter the pores of the foam carrier 42 and multiply, but by stirring the culture solution 41, the liquid medium and each foam carrier 42 come into good contact, and the ventilation pipe 30 The more air supplied comes into good contact with the foamed carrier 42, promoting proliferation.
本考案の培養装置によれば、培養槽内の培養液
が確実に撹拌され、微生物等と液体培地あるいは
空気とがよく接触し、微生物等の増殖が促進され
る。培養液中に発泡担体を分散せしめて、各発泡
担体内で微生物等を増殖させる場合には、回転体
は培養液内の攬拌に際して、各発泡担体をせん
断・損傷するおそれがない。
According to the culture device of the present invention, the culture solution in the culture tank is reliably stirred, the microorganisms etc. come into good contact with the liquid medium or air, and the growth of the microorganisms etc. is promoted. When the foamed carriers are dispersed in the culture solution and microorganisms are grown in each foamed carrier, there is no risk of the rotating body shearing or damaging each of the foamed carriers when stirring the culture solution.
第１図は本考案の培養装置の一例を示す縦断面
図、第２図は回転板の一例を示す平面図である。
１０……培養槽、１１……蓋部、１２……底面
部、２０……回転体、２１……回転板、２２……
突部、３０……通気管、４１……培養液、４２…
…発泡担体。
FIG. 1 is a longitudinal sectional view showing an example of the culture apparatus of the present invention, and FIG. 2 is a plan view showing an example of a rotating plate. DESCRIPTION OF SYMBOLS 10... Culture tank, 11... Lid part, 12... Bottom part, 20... Rotating body, 21... Rotating plate, 22...
Projection, 30... Ventilation pipe, 41... Culture solution, 42...
...foam carrier.
Claims (1)
された培養液を培養槽内に投入し、回転体によ
り撹拌して微生物、動植物培養細胞等を増殖さ
せる培養装置であつて、前記回転体は、培養槽
内の底面部に近接し、その上面に上方に突出す
る突部が設けられた回転板を具備する培養装
置。 ２ 前記培養液内に発泡担体が分散されている実
用新案登録請求の範囲第１項に記載の培養装
置。 ３ 前記発泡担体は、ウレタンフオームにて形成
されている実用新案登録請求の範囲第２項に記
載の培養装置。[Scope of Claim for Utility Model Registration] 1. A culture device in which a culture solution mixed with microorganisms, cultured animal and plant cells, etc. to be cultured is put into a culture tank and stirred by a rotating body to proliferate microorganisms, cultured animal and plant cells, etc. In the culture apparatus, the rotating body is provided with a rotating plate that is close to the bottom of the culture tank and has a protrusion that projects upwardly on the top surface of the rotating plate. 2. The culture device according to claim 1, wherein a foamed carrier is dispersed in the culture solution. 3. The culture device according to claim 2, wherein the foam carrier is made of urethane foam.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14626785U JPH0226320Y2 (en) | 1985-09-24 | 1985-09-24 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14626785U JPH0226320Y2 (en) | 1985-09-24 | 1985-09-24 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6253699U JPS6253699U (en) | 1987-04-03 |
| JPH0226320Y2 true JPH0226320Y2 (en) | 1990-07-18 |
Family
ID=31058576
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14626785U Expired JPH0226320Y2 (en) | 1985-09-24 | 1985-09-24 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0226320Y2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR900005770B1 (en) * | 1988-09-20 | 1990-08-11 | 한국과학기술원 | Producing and isolations method of plant cell 2th product for using absorption substance |
-
1985
- 1985-09-24 JP JP14626785U patent/JPH0226320Y2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6253699U (en) | 1987-04-03 |
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