CN2236493Y - Biologic reactor for cell culture - Google Patents
Biologic reactor for cell culture Download PDFInfo
- Publication number
- CN2236493Y CN2236493Y CN 95232664 CN95232664U CN2236493Y CN 2236493 Y CN2236493 Y CN 2236493Y CN 95232664 CN95232664 CN 95232664 CN 95232664 U CN95232664 U CN 95232664U CN 2236493 Y CN2236493 Y CN 2236493Y
- Authority
- CN
- China
- Prior art keywords
- cell culture
- reactor
- barrel body
- culture
- cylindrical shell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
- C12M29/08—Air lift
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/16—Screw conveyor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Abstract
The utility model relates to a biologic reactor for cell culture. The biologic reactor comprises a barrel body with a jacket, and a mixer assembled in the barrel body, wherein, the spiral mixer and a microporous film permeation device are arranged in the guide barrel of the barrel body; a viewing mirror is arranged at the lower part position of the barrel body mutually corresponding to the top of the guide barrel. The biologic reactor effectively overcomes the defect of the prior art, and has the characteristics of circulating current mixing, notable decrease of mixing shearing force, infiltration oxygen supply, enhanced cell culture effect and wide range of application.
Description
The utility model relates to a kind of bio-reactor that is used for microorganism, animal and plant cell cultures.
At present, the bio-reactor for microorganism, animal and plant cell cultures are used especially for the bio-reactor in the laboratory, mainly adopts the mechanical agitation type structure.Mechanical stirring device in the reactor flat oar single-blade generally commonly used or many paddle stirrers, the stirring shearing force of utilizing its high speed rotating to produce drives culture and flows.Because of its shearing force in the process of mixing is bigger, fragile by cultured cells; The fragmentation of the formed air pocket of high speed rotating also can produce damaging action to sensitive cells, has both made to reduce speed of rotation also the culture effect of pair cell is influential.In addition, the sealing leak of mechanical drive and the pollution that produces also is the important factor in order that cell cultures can not be ignored.For addressing the above problem, magnetic agitation bio-reactor and airlift bioreactor have appearred again.Though the former can avoid the sealing leak pollution problem, stir the disadvantage that shearing force is big, damage culturing cell but still exist; The latter has overcome mechanical agitation type structure inherent defective, and it mainly is applicable to the cell culture system bigger to oxygen requirement, as to cultivation of high aerobism microorganism cells etc.Yet this reactor still is unsuitable for some oxygen requirements and little and to the cultivation of the more sensitive animal and plant cell of bubble breaking, the cultivation of above-mentioned several reactor pair cells does not all reach the ideal culture effect.Current laboratory is to the tetchy animal cell culture of shearing force, and general adopt " rolling bottle " cultivated, and the reaction conditions that this " rolling bottle " pair cell is cultivated is difficult to realize strict control, more can't be used for large-scale cell cultures.
The purpose of this utility model provides a kind of biological reactor for cell culture of unique properties, it has effectively overcome the shortcoming of prior art, has the advantages that circulation mixes, significantly reduces to stir shearing force, infiltration oxygen supply, raising cell cultures effect and widen range of application.
The purpose of this utility model is achieved in that this device comprises the cylindrical shell that has chuck and organizes its interior agitator, the ventilative device of screw mixer and microporous membrane is set in the guide shell of its middle cylinder body, visor is installed on cylindrical shell top and guide shell top opposite position.
Owing in the guide shell of the present utility model screw mixer is set, minimum speed when culture mixes is reduced to 18 rev/mins for 84 rev/mins by existing flat paddle stirrer, so fundamentally avoided the destruction of the bigger stirring shearing force of single-blade or many paddle stirrers generation, utilize the effect of spiral impellent, force culture in guide shell, to flow downward, driving between cylindrical shell and the guide shell culture in the annular space simultaneously flows from bottom to top, thereby in reactor, realize circulating mixing in order also 14 seconds when mixing of required average shear rate when mixing
-1Reduce to 3 seconds
-1, can reach the purpose of cell suspension culture, loss cell is minimum, helps cell and grows fast.Adopt the ventilative device infiltration of microporous membrane oxygen system in addition in the guide shell, avoided the formation of air pocket and consequent bubble breaking damaging action sensitivity cell.Therefore, adopt new-type bioreactor, both made tetchy animal cell culture of shearing force and large scale culturing, also all very suitable, significantly to widen its range of application and improve the cell cultures effect, this device is very easily applied.
The utility model is described in further detail below in conjunction with accompanying drawing.
Fig. 1 is a kind of concrete structure synoptic diagram of the utility model.
Describe concrete structure of the present utility model in detail according to Fig. 1.This device comprises parts such as screw mixer 1 in the cylindrical shell 9 that has chuck 8, assembling cylindrical shell 9 guide shells 3, the ventilative device 2 of microporous membranes.Wherein cover in advance to be provided with on 6 and add mouth 5, discharge outlet 4, the cylindrical shell 9 that the bottom is provided with thief hole 13 is assembled on the bearing 11.The recirculated water of constant temperature system control is housed in the chuck 8,10 enters by upper outlet and flow out from entering the mouth, to adjust the control culture temperature.Screw mixer 1 drives by transmission mechanism 12 and rotates, and the bottom or the top of its assembling guide shell 3 can determine according to actual needs that the spiral stirrer 1 of present embodiment is positioned at the bottom of guide shell 3.Generally, place the top or the bottom of guide shell 3 with the demand of the ventilative device 2 of microporous membrane by the infiltration oxygen supply.The ventilative device 2 of the microporous membrane of present embodiment places the top of guide shell 3.Be liquid level in the observing response device and culture mobility status, visor 7 be installed on cylindrical shell 9 tops and guide shell 3 top opposite positions.Also can on cylindrical shell top cover 6, visor be set for observing conveniently.
During operation, start transmission mechanism 12 and drive screw mixer 1 rotation, under its rotary impetus effect, the culture that modulates in advance flows downward in the guide shell 3 of cylindrical shell 9, flow out from guide shell 3 bottoms again, enter in the annular space between cylindrical shell 9 and the guide shell 3 and upwards flow, form and circulate mixing.Minimum speed when its culture mixes can reach 18 rev/mins, and the average shear rate when mixing is 3 seconds
-1, and the existing minimum speed of flat oar stirred bioreactor (10L) when mixing is 84 rev/mins, the average shear rate when mixing is 14 seconds
-1.Mobile mixed effect under same reaction conditions, this device obviously are better than existing like product.If cell is aerobic, the aseptic gas that infiltrates from the ventilative device 2 of the microporous membrane on guide shell 3 tops enters oxygen supply in the above-mentioned circulation blended culture with diameter less than the microbubble of 0.1mm.Its air demand can be regulated, and tail gas is discharged from discharge outlet 4.The required reaction conditions of culture can by each regulation system control of reactor, handle getting final product routinely according to actual requirement.
Claims (3)
1, a kind of biological reactor for cell culture comprises the cylindrical shell that has chuck and organizes agitator in it, it is characterized in that in the guide shell of cylindrical shell screw mixer and the ventilative device of microporous membrane being set, and on cylindrical shell top and guide shell top opposite position visor is installed.
2, bio-reactor according to claim 1 is characterized in that the ventilative device of microporous membrane places guide shell top.
3, bio-reactor according to claim 1 is characterized in that the cylindrical shell top cover is provided with visor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95232664 CN2236493Y (en) | 1995-11-07 | 1995-11-07 | Biologic reactor for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95232664 CN2236493Y (en) | 1995-11-07 | 1995-11-07 | Biologic reactor for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2236493Y true CN2236493Y (en) | 1996-10-02 |
Family
ID=33876975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95232664 Expired - Fee Related CN2236493Y (en) | 1995-11-07 | 1995-11-07 | Biologic reactor for cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2236493Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100408670C (en) * | 2001-03-09 | 2008-08-06 | 天然植入物公司 | Bio-reactor for tissue cultivated in the form of a thin layer and uses thereof |
| CN101182458B (en) * | 2007-11-28 | 2011-04-20 | 南京工业大学 | Dynamic heat-pipe mixing bioreactor used for multiple biological reactions |
| CN103060189A (en) * | 2013-01-29 | 2013-04-24 | 南京工业大学 | Stirring type reactor with built-in plate-fin heat exchanger and temperature control method |
| CN111662826A (en) * | 2020-06-23 | 2020-09-15 | 肇庆大华农生物药品有限公司 | Bioreactor for high-density large-scale cell culture |
| CN111676136A (en) * | 2020-06-23 | 2020-09-18 | 肇庆大华农生物药品有限公司 | Bioreactor for improving antigen yield |
-
1995
- 1995-11-07 CN CN 95232664 patent/CN2236493Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100408670C (en) * | 2001-03-09 | 2008-08-06 | 天然植入物公司 | Bio-reactor for tissue cultivated in the form of a thin layer and uses thereof |
| CN101182458B (en) * | 2007-11-28 | 2011-04-20 | 南京工业大学 | Dynamic heat-pipe mixing bioreactor used for multiple biological reactions |
| CN103060189A (en) * | 2013-01-29 | 2013-04-24 | 南京工业大学 | Stirring type reactor with built-in plate-fin heat exchanger and temperature control method |
| CN111662826A (en) * | 2020-06-23 | 2020-09-15 | 肇庆大华农生物药品有限公司 | Bioreactor for high-density large-scale cell culture |
| CN111676136A (en) * | 2020-06-23 | 2020-09-18 | 肇庆大华农生物药品有限公司 | Bioreactor for improving antigen yield |
| CN111662826B (en) * | 2020-06-23 | 2023-07-28 | 肇庆大华农生物药品有限公司 | Bioreactor for high-density large-scale cell culture |
| CN111676136B (en) * | 2020-06-23 | 2023-08-25 | 肇庆大华农生物药品有限公司 | Bioreactor for improving antigen yield |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |