JPH02251766A - Measuring method of fructosamine - Google Patents
Measuring method of fructosamineInfo
- Publication number
- JPH02251766A JPH02251766A JP7339189A JP7339189A JPH02251766A JP H02251766 A JPH02251766 A JP H02251766A JP 7339189 A JP7339189 A JP 7339189A JP 7339189 A JP7339189 A JP 7339189A JP H02251766 A JPH02251766 A JP H02251766A
- Authority
- JP
- Japan
- Prior art keywords
- fructosamine
- measurement
- phenazinemethosulfate
- ascorbic acid
- anionic surfactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- IXZISFNWUWKBOM-ARQDHWQXSA-N fructosamine Chemical compound NC[C@@]1(O)OC[C@@H](O)[C@@H](O)[C@@H]1O IXZISFNWUWKBOM-ARQDHWQXSA-N 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000005259 measurement Methods 0.000 claims abstract description 15
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 claims abstract description 9
- 125000003831 tetrazolyl group Chemical group 0.000 claims abstract description 9
- 239000003945 anionic surfactant Substances 0.000 claims abstract description 8
- 108091006149 Electron carriers Proteins 0.000 claims description 8
- MASUWVVNWALEEM-UHFFFAOYSA-M 1-methoxy-5-methylphenazin-5-ium;methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2N=C3C(OC)=CC=CC3=[N+](C)C2=C1 MASUWVVNWALEEM-UHFFFAOYSA-M 0.000 claims description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract description 28
- 229960005070 ascorbic acid Drugs 0.000 abstract description 14
- 235000010323 ascorbic acid Nutrition 0.000 abstract description 14
- 239000011668 ascorbic acid Substances 0.000 abstract description 14
- 230000035945 sensitivity Effects 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 5
- 230000000087 stabilizing effect Effects 0.000 abstract description 2
- 230000027756 respiratory electron transport chain Effects 0.000 abstract 3
- FSVCQIDHPKZJSO-UHFFFAOYSA-L nitro blue tetrazolium dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 FSVCQIDHPKZJSO-UHFFFAOYSA-L 0.000 abstract 2
- 239000003638 chemical reducing agent Substances 0.000 abstract 1
- 229910052740 iodine Inorganic materials 0.000 abstract 1
- 239000011630 iodine Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 description 15
- -1 glycated hemoglobin Chemical compound 0.000 description 10
- ZYDGCYWJDWIJCS-UHFFFAOYSA-N 1-methoxyphenazine Chemical compound C1=CC=C2N=C3C(OC)=CC=CC3=NC2=C1 ZYDGCYWJDWIJCS-UHFFFAOYSA-N 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 238000007796 conventional method Methods 0.000 description 4
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 3
- 239000004711 α-olefin Substances 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 229940047035 ascorbic acid 20 mg Drugs 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- 102000017011 Glycated Hemoglobin A Human genes 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 150000008051 alkyl sulfates Chemical class 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 108091005995 glycated hemoglobin Proteins 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 1
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
- ODUCDPQEXGNKDN-UHFFFAOYSA-N nitroxyl Chemical compound O=N ODUCDPQEXGNKDN-UHFFFAOYSA-N 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229940104261 taurate Drugs 0.000 description 1
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、血清・血漿中のフルクトサミンの効率的な測
定法に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an efficient method for measuring fructosamine in serum/plasma.
フルクトサミンは、現在糖尿病診断のマーカとしてグリ
コヘモグロビンと同様、たいへん注目されており、最近
その検査が急速に増えてきた。グリコヘモグロビンが、
過去２力月間の血糖状態を反映すると言われるが、フル
クトサミンは１〜２週間の血糖状態を反映すると言われ
る。現在フルクトサミンの測定法として、血清中の蛋白
質がグルコースと結合して生ずるケトアミン（フルクト
サミン）が、弱アルカリ溶液中でテトラゾリウム塩を還
元して生ずるホルマザン発色を利用して測定する方法が
よく用いられている。Fructosamine, like glycated hemoglobin, is currently attracting a lot of attention as a marker for diabetes diagnosis, and the number of tests for it has increased rapidly recently. Glycohemoglobin is
It is said that it reflects blood sugar status over the past two months, but fructosamine is said to reflect blood sugar status for one to two weeks. Currently, a commonly used method for measuring fructosamine is to use formazan coloring, which is produced when ketoamine (fructosamine), which is produced when proteins in serum combine with glucose, is reduced to a tetrazolium salt in a weakly alkaline solution. There is.
上述した従来の方法は、システィンやグルタチオン等の
ＳＨ化合物、アスコルビン酸等の還元物質の影響を受け
る点が非常に問題とされている。The conventional method described above has a serious problem in that it is affected by SH compounds such as cysteine and glutathione, and reducing substances such as ascorbic acid.
特に、アスコルビン酸による影響が大きく、試薬中にア
スコルビン酸を加えただけでもかなり強く発色する。ア
スコルビン酸の影響を避けるために、通常、アスコルビ
ン酸の発色を終わらせた後測定ポイントを２点選んでそ
の吸光度差をとって測定されている。しかし、アスコル
ビン酸の発色は、完結するまで１０〜１５分かかり、さ
らにブランク値が高いレベルから測定しなければならず
、正確な測定が難しい上、最近の測定時間の短い自動分
析装置にはのりにくいという問題があった。In particular, the effect of ascorbic acid is large, and even just adding ascorbic acid to the reagent produces a fairly strong color. In order to avoid the influence of ascorbic acid, measurement is usually carried out by selecting two measurement points after the ascorbic acid has finished coloring and taking the difference in absorbance. However, the color development of ascorbic acid takes 10 to 15 minutes to complete, and furthermore, the blank value must be measured from a high level, making accurate measurement difficult, and it is difficult to use modern automated analyzers with short measurement times. The problem was that it was difficult.
さらにもう一つの大きな問題は、充分な感度が得られな
い点であった。Yet another major problem was that sufficient sensitivity could not be obtained.
また、従来の方法では、ｐＨ１・０〜１１でＮＴＢ等に
トロブルーテトラゾリウム塩）のテトラゾリウム塩に検
体中のフルクトサミンを反応させて測定を行っている。Furthermore, in the conventional method, measurement is performed by reacting fructosamine in the specimen with a tetrazolium salt such as NTB (troblue tetrazolium salt) at pH 1.0 to 11.
この反応に於て、ＰＭＳ等の電子伝達体を介せず直接Ｎ
ＴＢを還元させるので充分な感度が得られない、ＰＨＩ
Ｏ以下では、フルクトサミンはほとんどテトラゾリウム
塩を還元できず感度を上げるためには、ｐＨ１０以上に
しなければならない、しかし反面ｐＨ１０以上にした場
合アスコルビン酸等の影響を大きく受けるようになる。In this reaction, N
PHI reduces TB and does not provide sufficient sensitivity.
Below 0, fructosamine is hardly able to reduce the tetrazolium salt, and in order to increase sensitivity, the pH must be set to 10 or higher.However, if the pH is set to 10 or higher, fructosamine becomes greatly affected by ascorbic acid, etc.
本発明は、特にｐＨ１０以上にこだわらずＰＭＳ（フェ
ナジンメトスルフエイト）、ｌ−メトキシ−ＰＭＳ （
１−メトキシフェナジンメトスルフエイト）等の電子伝
達体を加えることにより従来の問題点を解決できるよう
にした方法を提供することを目的とするものである。The present invention is applicable to PMS (phenazine methosulfate), l-methoxy-PMS (
The purpose of this invention is to provide a method in which the problems of the conventional method can be solved by adding an electron carrier such as 1-methoxyphenazine methosulfate.
また、本発明は、従来のアスコルビン酸等の還元物質の
影響を回避するとともに高感度に測定できるようにした
方法を提供することを目的とするものである。Another object of the present invention is to provide a method that avoids the influence of conventional reducing substances such as ascorbic acid and enables highly sensitive measurement.
上記目的を達成するために、本発明方法においては、テ
トラゾリウム塩を用いてフルクトサミンを定量する方法
において、アニオン性界面活性剤の存在下で電子伝達体
を加えることによって測定するようにしたものである。In order to achieve the above object, in the method of the present invention, fructosamine is determined by adding an electron carrier in the presence of an anionic surfactant in the method of quantifying fructosamine using a tetrazolium salt. .
本発明で使用されるテトラゾリウム塩には、■ＮＴ （
３−（バラーヨウドフェニル）−２−（パラニトロフェ
ニル）−５−フェニル−２Ｈ−テトラゾリウムクロライ
ド）、ＮＴＢにトロブルーテトラゾリウム）などがある
。The tetrazolium salt used in the present invention includes ■NT (
3-(variodophenyl)-2-(paranitrophenyl)-5-phenyl-2H-tetrazolium chloride), and NTB (troblue tetrazolium).
本発明に用いられる電子伝達体としては、フェナジンメ
トスルフエイト、！−メトキシーフェナジンメトスルフ
エイト等が挙げられる。As the electron carrier used in the present invention, phenazine methosulfate,! -Methoxyphenazine methosulfate and the like.
また、上記フェナジンメトスルフエイトや１−メトキシ
−フェナジンメトスルフェイト等の電子伝達体を加えた
場合のブランク反応を安定させるためにアニオン性界面
活性剤が非常に効果的でありより高感度かつ精度よく測
定できるようになるものである。In addition, anionic surfactants are very effective in stabilizing the blank reaction when electron carriers such as phenazine methosulfate and 1-methoxy-phenazine methosulfate are added, resulting in higher sensitivity and precision. This allows for better measurement.
本発明にを効なアニオン性界面活性剤として次のような
ものを用いることができる。すなわちアルキル硫酸塩、
ポリオキシエチレンアルキルエーテル硫酸塩、Ｎ−アシ
ルアミノ酸とその塩、Ｎ−アシルメチルタウリン塩、ポ
リオキシエチレンアルキルエーテル酢酸塩、アルキルス
ルホカルボン酸塩、α−オレフィンスルホン酸塩、アル
キルリン酸塩、ポリオキシエチレンアルキルエーテルリ
ン酸塩等用いることができる。The following can be used as anionic surfactants effective in the present invention. i.e. alkyl sulfate,
Polyoxyethylene alkyl ether sulfate, N-acyl amino acid and its salt, N-acylmethyl taurate, polyoxyethylene alkyl ether acetate, alkyl sulfocarboxylate, α-olefin sulfonate, alkyl phosphate, poly Oxyethylene alkyl ether phosphate and the like can be used.
電子伝達体及びアニオン性界面活性剤を適宜第一試薬又
は、第二試薬に添加することによって従来のようにアス
コルビン酸の影響を避けるために特に待ち時間をおかな
いで短時間に測定が可能になり反応時間の短い最近の自
動分析装置に容易に適用できる。By adding an electron carrier and anionic surfactant to the first reagent or second reagent as appropriate, measurements can be carried out in a short time without any waiting time in order to avoid the influence of ascorbic acid as in conventional methods. It can be easily applied to modern automatic analyzers with short reaction times.
（実施例）
以下に本発明の実施例を挙げてさらに具体的に説明する
が、本発明がこれらの実施例の記載に限定されるもので
ないことは勿論である。(Examples) The present invention will be described in more detail below with reference to Examples, but it goes without saying that the present invention is not limited to the description of these Examples.
実施例１
試薬の組成
第一試薬：
トリス−塩酸緩衝液・・・・・・！ＯｍＭ、ｐＨ８，０
１−ｍ−ＰＭＳ・・・・・・・・・・・・・・・・・・
・・・０．０７５ｍＭ第二試薬：
炭酸緩衝液・・・・・・・・・・・・・・・５０ｍＭ、
ｐＨ１０，３ＮＴＢ・・・ ・・・・・・・・・・・
・・・・・・・・・・・・・・・・０．７５ｍＭα−オ
レフィンスルホン酸ナトリウム
０．５％
（ＮＴＢ：ニトロブルーテトラゾリウム）第 Ｅ薬２ｍ
ｊ！にアスコルビン酸２０ ｍ ｇ ／ ｄｌの試料を
１００μ！加え、３７°Ｃ５分間反応させたのち、第二
試薬１ｍｌ加え、３７°Ｃで反応させる＊ ５４０ ｎ
ｍで吸光度を測定し反応の経時変化を記録し、第１図に
示した。Example 1 Reagent composition First reagent: Tris-HCl buffer...! OmM, pH8.0
1-m-PMS・・・・・・・・・・・・・・・・・・
...0.075mM second reagent: carbonate buffer ......50mM,
pH10.3NTB... ・・・・・・・・・・・・
・・・・・・・・・・・・・・・・0.75mM Sodium α-olefin sulfonate 0.5% (NTB: Nitroblue Tetrazolium) Drug E 2m
j! Add a 100μ sample of ascorbic acid 20 mg/dl to it! Add and react at 37°C for 5 minutes, then add 1ml of the second reagent and react at 37°C* 540 n
The absorbance was measured at m, and the time course of the reaction was recorded, and is shown in FIG.
比較例！
試薬：
炭酸緩衝液・・・・・・・・・・・・１００ｍＭ、ｐＨ
１０，３ＮＴＢ・・・・・・ ・・ ・・・・
・・・・・・ ０． ２５ｍＭ試薬２ｍｆにアスコル
ビン酸２０ ｍ ｇ ／ ｄ ｉ、の試料を１００μ２
加え、３７°Ｃで反応させる。５４０ｎｍ吸光度を測定
し反応の経時変化を記録し、第１図に示した。Comparative example! Reagent: Carbonate buffer 100mM, pH
10,3NTB・・・・・・ ・・・・
・・・・・・ 0. 100 μ2 of a sample of ascorbic acid 20 mg/di in 2 mf of 25 mM reagent.
Add and react at 37°C. The absorbance at 540 nm was measured to record the time course of the reaction, which is shown in FIG.
第１図に示されるごとく、 比較例１に於いては、アス
コルビン酸の反応が急激に進行するが本発明の実施例１
では、アスコルビン酸の反応が著しく抑制されているこ
とがわかる。As shown in FIG. 1, in Comparative Example 1, the reaction of ascorbic acid progresses rapidly, but in Example 1 of the present invention, the reaction of ascorbic acid proceeds rapidly.
It can be seen that the reaction of ascorbic acid is significantly suppressed.
実施例２
第一試薬：
炭酸緩衝液・・・・・・・・・・・・・・・１００ｍＭ
、ｐＨ９，８ＮＴＢ ・・・ ・・・・・・・
・ ０．３１ｍＭα−オレフィンスルホン酸ナトリ
ウム
０、４％
第二試薬：
炭酸緩衝液・・・・・・・・・・・・・・・１００ｍＭ
、ｐＨ９，８１−ｍ−ＰＭＳ・・・・・・・・・・・・
・・・・・・・・・０．０７５ｍＭ第一試薬１ｍｆに検
体を５０μ！加え、３７°Ｃ５分間反応させたのち、第
二試薬０．２５ｍｆ加え、３７°Ｃで反応させる。５４
０ｎｍで１分間あたりの吸光度変化を測定した。Example 2 First reagent: Carbonate buffer・・・・・・・・・・・・100mM
, pH9,8NTB ・・・・・・・・・
・ 0.31mM α-olefin sodium sulfonate 0.4% Second reagent: Carbonate buffer・・・・・・・・・・・・100mM
, pH9,81-m-PMS・・・・・・・・・・・・
・・・・・・・・・50μ of sample in 1mf of 0.075mM first reagent! After adding and reacting at 37°C for 5 minutes, 0.25mf of the second reagent was added and reacting at 37°C. 54
The change in absorbance per minute was measured at 0 nm.
比較例２
試薬：
炭酸緩衝液・・・・・・・・・・・・１００ｍＭ、ｐＨ
１０，３ＮＴＢ ・・・ ・・・ ・・・・
・・・・・０．２５ｍＭ試薬１ｍｌに検体を５０μ！加
え、３７°Ｃで反応させる。５４０ｎｍで１分間あたり
の吸光度変化を測定する。Comparative Example 2 Reagent: Carbonate buffer 100mM, pH
10.3NTB ・・・ ・・・ ・・・・
...50μ of sample in 1ml of 0.25mM reagent! Add and react at 37°C. Measure the absorbance change per minute at 540 nm.
実施例２及び比較例２の吸光度変化を第１表に示した。Table 1 shows the absorbance changes of Example 2 and Comparative Example 2.
第１表から本発明の実施例２に於ては測定感度が大きく
改善されることがわかった。From Table 1, it was found that the measurement sensitivity was greatly improved in Example 2 of the present invention.
第１表
注）検体１（フルクトサミン値２．２ｍＭ）検体２（フ
ルクトサミン値４．４ｍＭ）〔発明の効果〕
以上のように、本発明によれば、ｐＨ１０以上にこだわ
らずＰＭＳ （フェナジンメトスルフエイト）、１−メ
トキシ−ＰＭＳ（１−メトキシフェナジンメトスルフエ
イト）等の電子伝達体を加えることにより、従来のアス
コルビン酸等の還元物質の影響を回避するとともに高感
度にフルクトサミンを測定できるという大きな効果を奏
する。Table 1 Note: Sample 1 (Fructosamine value 2.2mM) Sample 2 (Fructosamine value 4.4mM) [Effects of the invention] As described above, according to the present invention, PMS (phenazine methosulfate) ), 1-Methoxy-PMS (1-methoxyphenazine methosulfate) and other electron carriers have the great effect of avoiding the effects of conventional reducing substances such as ascorbic acid and enabling high-sensitivity measurement of fructosamine. play.
第１図は、実施例１及び比較例１における反応の経時変
化を示すグラフである。
特許出願人 三光純薬株式会社FIG. 1 is a graph showing changes in reaction over time in Example 1 and Comparative Example 1. Patent applicant: Sanko Junyaku Co., Ltd.
Claims (2)
する方法において、アニオン性界面活性剤の存在下で電
子伝達体を加えることによって測定することを特徴とす
るフルクトサミンの測定法。(1) A method for quantifying fructosamine using a tetrazolium salt, which is characterized in that the measurement is carried out by adding an electron carrier in the presence of an anionic surfactant.
メトキシ−フェナジンメトスルフェイト等であることを
特徴とする請求項（１）記載のフルクトサミンの測定法
。(2) The electron carrier is phenazine methosulfate, 1-
The method for measuring fructosamine according to claim 1, characterized in that the method is methoxy-phenazine methosulfate or the like.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1073391A JP2694004B2 (en) | 1989-03-24 | 1989-03-24 | Method for measuring fructosamine in serum or plasma |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1073391A JP2694004B2 (en) | 1989-03-24 | 1989-03-24 | Method for measuring fructosamine in serum or plasma |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02251766A true JPH02251766A (en) | 1990-10-09 |
| JP2694004B2 JP2694004B2 (en) | 1997-12-24 |
Family
ID=13516849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1073391A Expired - Lifetime JP2694004B2 (en) | 1989-03-24 | 1989-03-24 | Method for measuring fructosamine in serum or plasma |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2694004B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02297065A (en) * | 1989-05-12 | 1990-12-07 | Toyobo Co Ltd | Reagent for measuring fructosamine |
| US8002966B2 (en) * | 2006-11-24 | 2011-08-23 | Health & Life Co., Ltd. | Biosensor, biostrip, and manufacture method of determination of uric acid by a non-enzymatic reagent |
| JP4879441B2 (en) * | 2000-05-23 | 2012-02-22 | 広司 早出 | Glycated protein measurement kit |
| CN112326639A (en) * | 2020-11-25 | 2021-02-05 | 迈克生物股份有限公司 | Kit and method for detecting fructosamine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6315168A (en) * | 1986-06-21 | 1988-01-22 | ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | Method of measuring serum fructosamine content in blood or sample induced from blood |
| JPS63201567A (en) * | 1987-02-17 | 1988-08-19 | Toyobo Co Ltd | Method for removing reducing material in vital ample liquid |
-
1989
- 1989-03-24 JP JP1073391A patent/JP2694004B2/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6315168A (en) * | 1986-06-21 | 1988-01-22 | ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | Method of measuring serum fructosamine content in blood or sample induced from blood |
| JPS63201567A (en) * | 1987-02-17 | 1988-08-19 | Toyobo Co Ltd | Method for removing reducing material in vital ample liquid |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02297065A (en) * | 1989-05-12 | 1990-12-07 | Toyobo Co Ltd | Reagent for measuring fructosamine |
| JP4879441B2 (en) * | 2000-05-23 | 2012-02-22 | 広司 早出 | Glycated protein measurement kit |
| US8002966B2 (en) * | 2006-11-24 | 2011-08-23 | Health & Life Co., Ltd. | Biosensor, biostrip, and manufacture method of determination of uric acid by a non-enzymatic reagent |
| CN112326639A (en) * | 2020-11-25 | 2021-02-05 | 迈克生物股份有限公司 | Kit and method for detecting fructosamine |
| CN112326639B (en) * | 2020-11-25 | 2024-01-05 | 迈克生物股份有限公司 | Kit and method for detecting fructosamine |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2694004B2 (en) | 1997-12-24 |
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