JPH02251765A - Dyeing device for immune reaction - Google Patents

Abstract

PURPOSE:To enable automation of dyeing treatment of an organism piece for observation of an immune reaction by a small quantity of treating solution, by dripping the treating solution for dyeing onto a part protruding from the lateral edge of slide glass on a pedestal element. CONSTITUTION:A pedestal element 2 has a width larger than the one of slide glass 4 on the lower surface of which an organism piece is stuck, and a bank- shaped part 3 having a very small height is formed in one end thereof. A support plate element 9 is made slightly higher than the bank-shaped part 3 provided in the other end part of said pedestal element 2. Dripping of a treating solution is conducted by a dripping means which takes out a treating solution vessel by a takeout arm and transfers it above the pedestal element 2. Besides, a washing solution is discharged from a discharging port 10 by a washing solution supply means made up of a supply pump 13 and a washing solution storage tank 15, while the treating solution containing the washing solution is exhausted from an exhaust port 11 by a treating solution exhaust means made up of a supply pump 17 and an exhaust solution tank 19.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Use) The immunoreaction staining device according to the present invention is used to analyze the immunoreaction of tissue pieces taken out from living bodies when diagnosing cancer in hospitals, etc. It is used to stain this tissue piece for observation.

(Prior Art) Immune reactions such as antigen-antibody reactions are used to diagnose diseases such as cancer.

When diagnosing a disease using immune reactions in this way, tissue pieces taken from a living body are attached to a slide glass, and the tissue pieces are sequentially brought into contact with a predetermined treatment solution to stain the tissue pieces.

(Problems to be Solved by the Invention) However, when immunoreaction staining is performed for the purpose described above, the following disadvantages occur.

That is, when staining is performed automatically, conventionally, the processing solution is placed in a beaker-shaped container, and the slide glass with the tissue piece pasted is immersed in the processing solution in this container, so that the tissue piece and the processing solution are mixed together. Although we try to keep them in contact with each other, it is unavoidable that the amount of processing liquid used increases.

Since many of the processing solutions used for immune reaction observation are expensive, it is undesirable that the amount of processing solution used increases in this way.

For this reason, in the past, tissue pieces were often dyed manually to reduce processing costs, but the dyeing process took a long time and sometimes required all-night work, so it was labor-intensive for the workers. From an environmental perspective, this is not desirable.

The immunoreaction staining apparatus of the present invention eliminates all of the above-mentioned inconveniences by ensuring that tissue pieces can be immunotreated with a small amount of processing liquid.

(Means for Solving the Problems) The immunoreaction staining device of the present invention has at least a portion thereof larger in width than the width of the slide glass on which the tissue piece is pasted on the lower surface, and has a slight height at one end. A pedestal part forming a bank-shaped part, a support plate part provided at the other end of the pedestal part and slightly higher than the bank-shaped part, and a support plate part that protrudes from the side edge of the slide glass on the upper surface of the pedestal part. a dripping means for dripping a dyeing treatment liquid onto the part; a cleaning liquid supply means for discharging a cleaning liquid from a discharge port opened at a position close to the bank-like part on the top surface of the pedestal; The treatment liquid discharging means discharges the treatment liquid from a discharge port opened in a close position.

Furthermore, in the immunoreaction staining apparatus of the present invention, the block having the above-mentioned bank-like part and having a pedestal part and a support plate part with a discharge port and a discharge port opened on the top surface is attached to the top surface of the support plate. It can be attached and detached at will.

At the same time, the end of the liquid supply path leading to the discharge port and the end of the drain path also leading to the discharge port are opened at the lower surface of the block, and the end of the cleaning liquid supply path leading to the cleaning liquid supply means is opened to the bottom of the block. , and an end of a processing liquid discharge path leading to the processing liquid discharge means are opened at the upper surface of the support plate.

When the block is placed on the upper surface of the support plate, the end of the liquid supply path and the end of the cleaning liquid supply path are the same as the end of the liquid drain path and the end of the processing liquid discharge path, respectively. It can be closely connected.

(Function) When staining a tissue piece taken out from a living body using the immunoreaction staining apparatus of the present invention configured as described above,
A slide glass with a tissue piece to be stained is placed between a bank-shaped part and a support plate provided at the end of the pedestal part, with the part to which the tissue piece is affixed facing the upper surface of the pedestal part of the block. To support. In this state, a thin wedge-shaped gap is formed between the lower surface of the slide glass and the upper surface of the pedestal, which gradually becomes wider as the distance from the bank-like portion increases.

When the slide glass is placed so as to cross over the bank-like part of the upper surface of the block and the support plate part, the dripping means is applied to the upper surface of the pedestal part exposed laterally from the side edge of the slide glass. Drop a predetermined treatment liquid.

As mentioned above, there is a space between the lower surface of the slide glass and the upper surface of the pedestal, which gradually becomes wider as it moves away from the bank.
Since a wedge-shaped thin gap is formed, the processing liquid dropped on the top surface of the pedestal spreads inside this gap due to capillary action and comes into contact with the tissue piece attached to the bottom surface of the slide glass.

In this way, when the tissue piece on the bottom surface of the slide glass was brought into contact with the processing solution for a predetermined period of time, the processing solution discharge means, which had been stopped until then, was activated and opened at a position close to the bank-shaped part. Drain the processing liquid from the outlet. After completion of discharging the processing liquid, the processing liquid discharging means is stopped, and the cleaning liquid supply means, which had been stopped until then, is operated for a predetermined period of time to discharge the cleaning liquid from the discharge port provided in the vicinity of the bank-shaped portion. do.

The cleaning liquid discharged from the discharge port reaches the gap between the lower surface of the slide glass and the upper surface of the pedestal part by capillary action, and performs the cleaning treatment of the staining treatment liquid adhering to the tissue piece pasted on the lower surface of the slide glass.

When the cleaning process for a predetermined period of time is completed, the processing liquid discharge means is started again to discharge the cleaning liquid in the gap between the bottom surface of the slide glass and the top surface of the pedestal, and then the next staining processing liquid is dripped. Do this.

Thereafter, the above-described operations are repeated to perform a predetermined dyeing process.

When a predetermined processing operation is completed, the block is removed from the upper surface of the support plate, the processing liquid adhering to the upper surface of the pedestal and the inside of the discharge port or outlet is washed away and then dried, in preparation for the next use.

When placing a block that has been rinsed and dried of the processing liquid on the top surface of the support plate, connect the end opening of the liquid supply path on the bottom of the block to the end of the cleaning liquid supply path on the top surface of the support plate, and connect the end opening of the liquid supply path on the bottom surface of the block to the drainage path on the bottom surface of the block. Align the end openings of the liquid supply path and the cleaning liquid supply path with the ends of the processing liquid discharge path on the top surface of the support plate, and align the end openings of the liquid supply path and the cleaning liquid supply path with the end opening of the drainage path and the processing liquid discharge path. The ends of each channel are connected in a fluid-tight manner.

As a result, by appropriately operating the cleaning liquid supply means and the cleaning liquid discharge means, the processing liquid can be freely supplied and discharged between the upper surface of the pedestal and the lower surface of the slide glass.

(Example) Next, the present invention will be explained in more detail while explaining the illustrated embodiment. However, before explaining the immunoreaction staining apparatus of the present invention, the prior invention (Japanese Patent Application No. 62-314192) By explaining the immunoreaction staining apparatus according to the present invention, the basic structure and operation of the immunoreaction staining apparatus according to the present invention will be explained.

Figures 1 to 7 show the immunoreaction staining device of the previous invention.
Fig. 1 is a perspective view with the dripping means omitted, and Fig. 2 is a perspective view showing the dripping means.
3 is a view similar to FIG. 2 showing the arrangement of the discharge port and the discharge port separately; FIG. 4 is a perspective view of the processing liquid container storage part constituting the dripping means; FIG. 5 is a side view of the processing liquid container, FIG. 6 is a side view of the processing liquid container take-out arm, and FIG. 7 is a plan view thereof.

A flat pedestal part 2 is formed at the center of the upper surface of the dyeing block 1 made of aluminum or the like, and at one end of this pedestal part 2 there is a bank having a slight height of about 0.2■■. A shaped portion 3 is formed over the entire width of this pedestal portion 2. The pedestal part 2 has a width W larger than the width W of the slide glass 4 on which the tissue piece is pasted on the lower surface, and when the middle part of the slide glass 4 is placed over the pedestal part 2, the pedestal part 2 Both ends in the width direction are exposed from both side edges of the slide glass 4.

At one end edge of the staining block 1, a recess 7 is provided on the inner surface of the wall 6 facing the bank-like part 3 via the drainage groove 5, into which one end of the slide glass 4 can be fitted without rattling. is formed for positioning when the slide glass 4 is placed over the pedestal portion 2.

On the other hand, the pedestal part 2 is provided opposite to the other end side of the pedestal part 2, that is, the side where the bank-shaped part 3 is not provided, via the drainage groove 8.
The support plate part 9 that supports the lower surface of the other end of the slide glass 4 placed on the slide glass is made slightly higher than the bank-like part 3, and the slide glass is supported at both ends by the bank-like part 3 and the support plate part 9. The glass 4 is slightly inclined (for example, about 1 degree) so that the height gradually increases from the bank-shaped part 3 toward the support plate part 9.

A discharge port 10 and a discharge port 11 are opened on the upper surface of the pedestal portion 2 at a position close to the bank-like portion 3.

The discharge port 10 is connected to a cleaning liquid storage tank 15 via a feed pipe 14 having a rotary switching valve 12 and a liquid pump 13 in the middle, thereby forming a cleaning liquid supply means for discharging the cleaning liquid. are doing. The rotary type switching valve 12 is used to select the destination to which the cleaning liquid is sent when staining different types of tissue pieces attached to a plurality of slide glasses 4 at the same time.

Further, the discharge port 11 is connected to a drainage tank 19 via a discharge pipe 18 having a rotary switching valve 16 and a liquid sending pump 17 in the middle, thereby discharging the processing liquid including the cleaning liquid. It constitutes a liquid discharge means.

Of the discharge ports 10 and 11 provided on the upper surface of the pedestal portion 2 near the bank-shaped portion 3, it is preferable that the discharge port 11 be provided as close to the bank-shaped portion 3 as possible. may be provided somewhat toward the center of the pedestal portion 2. In other words, the discharge port 10 and the discharge port 11 may be arranged side by side as shown in FIGS. 1 and 2, or as shown in FIG. They may also be arranged vertically.

The dripping means for dripping the staining treatment liquid onto the upper surface of the pedestal portion 2 on the portion protruding from both side edges of the slide glass 4 is a fourth dripping means.
A processing liquid container 21 as shown in FIG. 5 stored in a processing liquid container storage section 20 as shown in the figure is taken out by a processing liquid container take-out arm 22 as shown in FIGS. It is configured so that it can be moved above the section 2 and the processing liquid can be dripped thereon.

The processing liquid container 21 has a push button 23 at the upper end, and by pushing the push button 23 downward, the processing liquid stored in the container body 24 can be freely dripped downward through the hoses 25 and 26.

On the other hand, the processing liquid container storage section 20, which stores a plurality of such processing liquid containers 21 and allows them to be taken out as needed, has a plurality of U-shaped parts on the periphery of a disk 28 that is rotatable about a vertical shaft 27. Notches 29, 29 are formed at equal intervals from each other, and the container body 24 made of an elastically deformable material such as vinyl can be fitted into each of the notches 29, 29.

Also, take out the processing liquid container 21 fitted into the notch 29, 29 of the processing liquid container storage part 20, move it above the pedestal part 2 covered with a predetermined slide glass 4, and remove the processing liquid. The processing liquid container take-out arm 22 that performs dripping includes a moving arm 30 that can be horizontally moved in two dimensions by a drive mechanism (not shown), and a gripping arm 31 provided at the tip of this moving arm 30.
and a pressing arm 32 which is provided above the gripping arm 31 so as to be able to rise and fall freely, and which presses the push button 23 at the upper end of the processing liquid container 21 downward when it is lowered.

When staining a tissue piece removed from a living body using the original immunoreaction staining device configured as described above,
A slide glass 4 with a tissue piece to be stained is inserted into a recess 7 formed in the wall 6 with one end of the slide glass 4, and the part of the slide glass in the center where the tissue piece is attached faces the top surface of the pedestal 2. In this state, it is supported by the upper edge of the bank-like part 3 provided at the end of the pedestal part 2 and the upper surface of the support plate part 9. In this state, a thin wedge-shaped gap is formed between the lower surface of the slide glass 4 and the upper surface of the pedestal section 2, which gradually becomes wider as the distance from the bank-shaped section 3 increases.

After the slide glass 4 is placed on the bank-shaped part 3 and the support plate part 9 in such a manner that it is placed across the slide glass 4, the movable arm 30 of the processing liquid container take-out arm 22 constituting the dripping means is moved to the processing liquid container storage area. The processing liquid container 21 is moved toward the storage section 20 and fitted into the notch 29 of the disc 28 of the storage section 20, and is gripped by the gripping arm 31 at the tip of the moving arm 30.

In this way, once the processing liquid container 21 is gripped by the gripping arm 31 at the tip of the moving arm 3o, the processing liquid container 21 should be brought into contact with the processing liquid by moving the moving arm 3o in a two-dimensional direction. It is moved above the slide glass 4 to which the tissue piece is attached.

Once the processing liquid container 21 has been moved above the predetermined slide glass 4, the pressing arm 3 provided at the tip of the moving arm 22
2 is lowered and the push button 23 provided at the upper end of the processing liquid container 21 is pressed downward, and the processing liquid sent out through the hoses 25 and 26 is pushed to the pedestal exposed laterally from the side edge of the slide glass 4. Drop onto the top surface of part 2.

Between the lower surface of the slide glass 4 that covers the pedestal section 2 and is supported so that a hook is passed between the upper edge of the bank-shaped section 3 and the upper surface of the support plate section 9, and the upper surface of the pedestal section 2, As mentioned above, since a wedge-shaped thin gap is formed that gradually widens as it moves away from the bank-shaped part 3, the processing liquid dropped on the top surface of the pedestal part 2 flows inside this gap by capillary action. As it goes, it comes into contact with the tissue piece attached to the lower surface of the slide glass 4.

In this way, when the tissue piece attached to the bottom surface of the slide glass 4 and the processing solution are brought into contact with each other for a predetermined period of time, the discharge port 1
1 and the drain tank 19 communicate with each other, and at the same time, the liquid feed pump 17 constituting the processing liquid discharge means, which had been stopped until then, is activated.
From the discharge port 11 opened at a position close to the bank-shaped part 3,
The processing liquid is discharged toward the drainage tank 19.

After the discharge of the processing liquid is completed, the liquid feeding pump 17 constituting the processing liquid discharge means is stopped, and the rotary switching valve 1 is changed so that the discharge port 10 and the cleaning liquid storage tank 15 communicate with each other.
At the same time, the liquid feeding pump 13 constituting the cleaning liquid supply means, which had been stopped until then, is operated for a predetermined period of time, and the cleaning liquid is discharged from the discharge port 10 provided in the vicinity of the bank-shaped portion 3.

The cleaning liquid discharged from the discharge port 10 reaches the gap between the lower surface of the slide glass 4 and the upper surface of the pedestal part 2 due to capillary action, and removes the stains attached to the tissue pieces attached to the lower surface of the slide glass 4. Perform a cleaning process for the processing liquid.

After completing the cleaning process for a predetermined period of time, the liquid pump 17 constituting the process liquid discharge means was started again to discharge the cleaning liquid in the gap between the lower surface of the slide glass 4 and the upper surface of the pedestal part 2. After that, the next dyeing solution is added dropwise.

Thereafter, the above-described operations are repeated to perform a predetermined dyeing process.

The basic structure and operation of the immunoreaction staining device of the present invention are as follows:
Although it is almost the same as the immunoreaction staining apparatus according to the previous invention described above, in the case of the present invention, a block having a pedestal part and a support plate part with a discharge port and a discharge port opened on the upper surface is attached to the support plate. By providing it detachably, it is easy to clean the upper surface of the pedestal after the dyeing work is completed.

That is, in the case of the immunoreaction staining apparatus of the present invention, a plurality of blocks 33 as shown in FIGS. 8 to 11 (
In the illustrated embodiment, 12 pieces) are arranged on the upper surface of the support plate 39.

A pair of support walls 34.35 are formed at intervals on the upper surface of each block 33.33 made of aluminum alloy or the like in the shape of a rectangular thick plate.
A plurality of (in the illustrated example, five) notches 36 and 37 are formed on the upper edge of each of the notches.

The cutouts 36 and 37 are for fitting the edge of a slide glass with a sample to be stained on the bottom surface without any play, and for positioning the slide glass. The notches 36, 36 of 34 and the notches 37, 37 of the other support wall 35 are formed at positions that align with each other in the width direction of the block 33 (vertical direction in FIG. 8).

Further, between the pair of support walls 34 and 35, each support wall 3
In the open position of the notch 36.37 formed in 4.35,
They each form a pedestal part 2.2. Each pedestal part 2.2
On one end side M (the right edge of the lower end in FIG. 8), there is an extending portion 3.
By forming 8.38, the width of this part (the part where the extension part 38 is formed) is made larger than the width of the slide glass, and the staining liquid dropped on the top surface of the extension part 38 is transferred to the pedestal. It can be freely fed into the gap between the upper surface of the section 2 and the lower surface of the slide glass.

On the upper surface of each pedestal part 2.2, a bank-shaped part 3 is formed at one end edge, as in the case of the previous invention described above, and a discharge port 10 and a discharge port 11 are formed in the vicinity of this bank-shaped part 3. The top end is open.

However, in the case of the dyeing apparatus of the present invention, the discharge port 10 and the discharge port 1
The lower ends of the blocks 1 and 1 are opened at the lower surface of the block 33, respectively.

At the same time, a switching valve 16 and a liquid pump 17 are provided at the end of the cleaning liquid supply path that leads to the cleaning liquid storage tank 15 via a feed pipe 14 having a switching valve 12 and a liquid pump 13 in the middle. Drainage tank 19 via drain pipe 18
The end of the processing liquid discharge path leading to the block 33.33
An opening is provided on the upper surface of the support plate 39 for placing the.

When each block 33.33 is placed on the upper surface of the support plate 39, the end of the liquid supply path on the lower surface of the block 33 and the end of the cleaning liquid supply path on the upper surface of the support plate 39 are liquid-tightly connected. At the same time, the end of the drain channel on the lower surface of the block 33 and the support plate 3
The end portion of the processing liquid discharge path on the upper surface of 9 is connected in a liquid-tight manner.

That is, one end edge of each block 33 (lower end in FIG. 8, it)
A notch 40 for positioning is formed in the center, and when the block 33 is placed on the top surface of the support plate 39 and the notch 40 is engaged with a protrusion provided on the top surface of the support plate 39, the above-mentioned feed The end of the liquid path and the end of the cleaning liquid supply path are connected in a liquid-tight manner, and at the same time, the end of the liquid drainage path and the end of the processing liquid discharge path are connected in a liquid-tight manner.

In addition, as a structure for liquid-tightly connecting the end of the liquid supply path and the end of the cleaning liquid supply path, or liquid-tightly connecting the end of the liquid drain path and the end of the processing liquid discharge path, Various conventionally known structures can be adopted, and if necessary, the end openings of the liquid supply path and the liquid drainage path and the end openings of the cleaning liquid supply path and the processing liquid discharge path can be connected to each other. Fit the concave and convex parts, and attach an O-ring to the fitting part.

Note that the stocker 41 shown in the lower half of FIG. (including the top surface)
In the stocker 41, which is surrounded by a heat insulating material, there are provided three disk-shaped processing liquid container storage parts 20 and 20, each of which rotates around a vertical axis.
0.20 by an appropriate angle and inserting the processing liquid container takeout arm 22 into the stocker 41 through the openings 42, 42 provided at three positions on the front surface of the stocker 41 (top surface in FIG. 12) to perform the desired processing. Take out the liquid container 21, move the taken out processing liquid container 21 upwards of the pedestal part 2,
Perform dripping work of processing liquid.

The function of staining a specimen attached to a glass slide using the immunoreaction staining apparatus of the present invention configured as described above is the same as that of the staining apparatus of the previous invention described above.

However, in the case of the dyeing apparatus of the present invention, once the predetermined dyeing process is completed, each block 33, 33 is removed from the upper surface of the support plate 39, and the upper surface of the pedestal part 2 and the discharge port 10 or the discharge port 11 are removed. Rinse off the processing liquid adhering to the inside, dry it, and prepare for the next use. This cleaning and drying work can be performed easily and efficiently by removing the block 33.

Once the treatment liquid has been washed away and dried, the blocks 33.33 are placed on the upper surface of the support plate 39. When each block 33.33 is placed on the upper surface of the support plate 39, the edges of the blocks 33 The notch 40 formed in the part and the support plate 3
By engaging the protrusion provided on the top surface of block 9, block 3
3, the end opening of the liquid supply path on the lower surface of the block 33 is connected to the end of the cleaning liquid supply path on the upper surface of the support plate 39, and the end opening of the liquid drainage path on the lower surface of the block 33 is connected to the end of the processing liquid discharge path on the upper surface of the support plate 39. The end opening of the liquid supply path, the end of the cleaning liquid supply path, and the end opening of the drainage path and the end of the processing liquid discharge path are respectively aligned and connected in a fluid-tight manner.

As a result, by appropriately operating the liquid feeding pump 13 that constitutes the cleaning liquid supply means and the liquid feeding pump 17 that constitutes the processing liquid discharge means, the processing liquid is supplied between the upper surface of the pedestal section 2 and the lower surface of the slide glass. It can be freely supplied and discharged, making it possible to stain a sample attached to the bottom surface of a slide glass.

Incidentally, if a lid made of a transparent plate material such as acrylic is placed above the support plate 39 to cover the upper part of all the blocks 1.1, drying of the processing liquid can be prevented. However, if a lid is provided, a through hole is formed in a portion of the lid where the processing liquid is dropped.

(Effects of the Invention) Since the immunoreaction staining device of the present invention is configured and operates as described above, it is possible to automatically stain tissue pieces for observing immune reactions with a small amount of processing solution. , it has a great effect in contributing to reducing the cost and labor saving required for immune reaction observation.

Further, after the dyeing process, it is possible to easily wash away the processing liquid adhering to the area to be dyed and then dry it.

[Brief explanation of drawings]

′! Figures J1 to J7 show the immunoreaction staining apparatus according to the previous invention, which is the basis of the present invention, and Figure 1 is a perspective view with the dropping means omitted, and Figure 2 is a view taken along A-A in Figure 1. Cross section, 3rd
The figure is a view similar to Figure 2 showing the separate arrangement of the discharge port and the discharge port, Figure 4 is a perspective view of the processing liquid container storage part that constitutes the dripping means, and Figure 'j45 is a side view of the processing liquid container. , FIG. 6 is a side view of the processing liquid container take-out arm, FIG. 7 is a plan view thereof, FIG. 8 is a plan view of a block used by being incorporated into the immunoreaction staining apparatus of the present invention, and FIG. BB sectional view of the figure,
FIG. 10 is a sectional view taken along the line CC, FIG. 11 is a sectional view of the same, and FIG. 12 is a plan view of the dyeing apparatus of the present invention. 1: Staining block, 2: Pedestal part, 3: Bank-shaped part, 4 Ni-Ride glass, 5: Drainage groove, 6: Wall part, 7: Recessed part, 8:
Drainage groove, 9: Support plate part, 10: Discharge port, 11: Discharge port,
12: Rotary type switching valve, 13: Liquid feeding pump, 14: Feed pipe, 15: Cleaning liquid storage tank, 16: Rotary type switching valve, 17: Liquid feeding pump, 18: Discharge pipe, 19: Drainage tank, 20: IA processing liquid container storage section 21; processing liquid container, 22: processing liquid container removal arm, 23; push button, 24 container bodies, 25.26: hose, 27: vertical shaft, 28: disc, 29: notch, 30 moving arm, 31: grasping arm, 32: pressing arm, 33 nib block, 34.35: support wall, 36.3
7: notch, 38: extension, 39: support plate, 40: notch, 41: stocker, 42: opening. Patent Applicant: Chiyoda Manufacturing Co., Ltd. Managing Director: Kinzo Yama (and 1 other person)Figure 2Figure 3Figure 4Figure 4 20C1''it! Figure 1I

Claims (2)

[Claims] (1) A pedestal part, at least a part of which has a width larger than the width of the slide glass on which the tissue piece is pasted, and a bank-like part having a slight height at one end; a support plate portion provided on the end side that is slightly higher than the bank-like portion; and a dripping means for dripping a staining treatment liquid onto a portion of the upper surface of the pedestal portion that protrudes from the side edge of the slide glass;
A cleaning liquid supply means for discharging cleaning liquid from a discharge port opened at a position close to the bank on the top surface of the pedestal, and a cleaning liquid supply means for discharging the processing liquid from a discharge port opened at a position close to the bank on the top surface of the pedestal. An immunoreaction staining apparatus comprising a treatment liquid discharge means, the block having the bank-like part and having a base part and a support plate part with a discharge port and a discharge port opened on the upper surface, and a support plate part. The cleaning liquid is removably attached to the upper surface of the block, and the end of the liquid supply path leading to the discharge port and the end of the drain path leading to the discharge port are opened at the bottom surface of the block, and the cleaning liquid is connected to the cleaning liquid supply means. When the end of the supply path and the end of the processing liquid discharge path leading to the processing liquid discharge means are opened on the upper surface of the support plate, and the block is placed on the upper surface of the support plate,
An immunoreaction staining device in which an end of a liquid supply path and an end of a cleaning liquid supply path, an end of a drainage path and an end of a processing liquid discharge path can be connected to each other in a fluid-tight manner. (2) Above the support plate, in the part that covers the top of the block,
2. The immunoreaction staining apparatus according to claim 1, further comprising a transparent and removable lid, and a through hole is formed in a portion of the lid through which the processing liquid is dropped.