JP2834787B2 - Dyeing apparatus and dyeing tray - Google Patents

Dyeing apparatus and dyeing tray

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JP2834787B2
JP2834787B2 JP1246510A JP24651089A JP2834787B2 JP 2834787 B2 JP2834787 B2 JP 2834787B2 JP 1246510 A JP1246510 A JP 1246510A JP 24651089 A JP24651089 A JP 24651089A JP 2834787 B2 JP2834787 B2 JP 2834787B2
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dyeing
upper surface
liquid
portion
liquid supply
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JPH03111035A (en
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松巳 戸谷
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株式会社千代田製作所
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【発明の詳細な説明】 (産業上の利用分野) この発明に係る染色装置と染色用トレーは、病院等で癌の診断を行なう場合等に於いて、生体から取り出した組織片の免疫反応を観察する為、この組織片を染色する場合等に利用する。 BACKGROUND OF THE INVENTION (FIELD OF THE INVENTION) dyeing apparatus and dyeing tray according to the invention, in the case for performing a diagnosis of cancer in a hospital or the like, the immune response of a tissue piece taken from a living body order to observe, to use in the case, such as the dyeing of this piece of tissue.

(従来の技術) 抗原抗体反応等の免疫反応を利用して、癌等の病気診断を行なう事が行なわれている。 Using the (prior art) immune response of the antigen-antibody reaction or the like, it has been made possible to perform the diagnosis of diseases such as cancer.

この様に免疫反応を利用して病気診断を行なう場合、 If this way in using the immune reaction is carried out for the diagnosis of diseases,
スライドガラスに生体から取り出した組織片を貼付し、 The slides were affixed tissue pieces taken out from the living body,
この組織片を所定の処理液に順番に接触させて、組織片の染色を行なう。 The tissue pieces are contacted sequentially to a predetermined processing liquid, performs staining of tissue specimens.

ところで、上述の様な目的で免疫反応の染色処理を行なう場合に於いて、従来は、処理液をビーカ状の容器に入れ、組織片を貼付したスライドガラスをこの容器内の処理液中に浸漬して、組織片と処理液とを接触させる様にしているが、使用する処理液の量が多くなる事が避けられない。 Incidentally, in the case of the dyeing process of the immune reaction in the above such purpose, conventionally, put the processing liquid on the beaker-like container, soaked slides was stuck pieces of tissue in the treatment liquid in the container to, but in the manner contacting the tissue pieces with the treatment liquid inevitably be increased the amount of the processing solution used.

免疫反応観察の為に使用する処理液は高価なものが多い為、この様に処理液の使用量が多くなる事は好ましくない。 Since the immune response observed treatment liquid used for the often expensive, it is not preferable to becomes large amount of such a processing solution.

この為従来は、処理コスト低減の為、手作業によって組織片の染色処理を行なう事が多いが、染色処理には長い時間を要し、場合によっては徹夜の作業になる為、作業員の労働環境の面から見て、やはり好ましくない。 For this reason the prior art, because of the processing cost reduction, but it is often performed the dyeing process of the tissue pieces by hand, it takes a long time to dyeing process, in some cases to become a work of staying up all night is, the labor of workers as seen from the surface of the environment, also undesirable.

この様な問題を解決する為、本発明者は先に、少ない処理液で、組織片の染色処理を確実に行なえる免疫反応染色装置を発明した(特開平1−155269号)。 To solve such problems, the present inventors have previously with less processing solution, invented reliably performed immunoreactive staining apparatus dyeing pieces of tissue (JP 1-155269).

この先発明に係る免疫反応染色装置は、第10〜12図に示す様に構成されている。 Immunoreactive staining apparatus according to the prior invention is configured as shown in 10-12 FIG.

1はアルミニウム等により造られた染色ブロックで、 1 is a stain block, made of aluminum or the like,
この染色ブロック1の上面中央部には、平坦な台座部2、2が形成されており、この台座部2、2の一端には、僅かな高さを有する土手状部3、3を、この台座部2、2の全幅に亙って形成している。 The upper central portion of the dyeing block 1 is formed with a flat base portion 2, 2, the one end of the base portion 2, 2, the bank-shaped portions 3, 3 having a small height, this It is formed over the entire width of the base portion 2, 2. 上記台座部2、2 The base portion 2, 2
は、下面に組織片を貼付したスライドガラス4、4の幅wよりも大きな幅Wを有し、スライドガラス4、4の中間部を台座部2、2の上方に被せた場合に、台座部2、 Has a greater width W than the width w of the slide glass 4, 4 attached tissue pieces on the lower surface, an intermediate portion of the slide glass 4,4 when overlaid above the pedestal 2, 2, the base portion 2,
2の幅方向両端部が、スライドガラス4、4の両側縁から露出する様にしている。 Both widthwise end portions of the two has the manner exposed from both side edges of the slide glass 4,4.

染色ブロック1の一端縁部で、上記土手状部3、3と排液溝5を介して対向する壁部6の内側面には、上記スライドガラス4、4の一端をがたつきなく嵌合自在な凹部7、7を形成して、スライドガラス4、4を台座部2、2の上方に被せる場合に於ける位置決めを図っている。 In one end edge portion of the dyeing block 1, the inner surface of the wall portion 6 which faces via the bank-like portions 3,3 and drainage ditch 5, without play an end of the slide glass 4,4 fitting forming a freely recess 7, 7, thereby achieving an in positioning when covering the slides 4,4 to the upper side of the pedestal 2, 2.

一方、台座部2、2の他端側、即ち、土手状部3、3 On the other hand, the other end of the base portion 2, 2, i.e., bank-shaped portions 3, 3
を設けていない側と排液溝8を介して対向して設けられ、台座部2、2に被せたスライドガラス4、4の他端部下面を支持する支持板部9は、上記土手状部3、3よりも僅かに高くして、両端を土手状部3、3と支持板部9とで支持されたスライドガラス4、4が、土手状部3、3から支持板部9に向かうに従って次第に高くなる様に、僅かに(例えば1度程度)傾斜する様にしている。 The through side and drainage ditch 8 is not provided disposed opposite the support plate 9 for supporting the other end lower surface of the slide glass 4, 4 over the base portion 2 and 2, said bank-like portion and slightly higher than 3,3, the slide glass 4, 4 is supported at both ends by the bank-like portions 3, 3 and the support plate portion 9, toward the bank-shaped portion 3, 3 supporting plate portion 9 as increasingly high, and the manner slightly (eg about 1 °) slope.

各台座部2、2の上面で、土手状部3、3に近い位置には、吐出口10、10と排出口11、11とが開口している。 In the upper surface of the base portion 2, 2, the position close to the bank-shaped portions 3, 3, the discharge port 10, 10 and outlet 11 and 11 is opened.
この内の吐出口10、10は、途中にロータリ式切換弁12と送液ポンプ13とを設けた送り込み管14を介して、洗浄液貯溜タンク15に通じさせる事で、洗浄液を吐出する洗浄液供給手段を構成している。 Discharge ports 10, 10 of this via the infeed tube 14 provided with a rotary selector valve 12 and the liquid supply pump 13 on the way, by providing communication to the cleaning liquid storage tank 15, the cleaning liquid supply means for discharging the washing liquid constitute a. この内のロータリ式切換弁 Rotary selector valve of this
12は、複数枚のスライドガラス4、4に貼付した異なる種類の組織片の染色処理を同時に行なう場合に、洗浄液を送る先を選択する為のものである。 12, when carried out was attached on a plurality of glass slides 4,4 different types of tissue piece dyeing simultaneously, it is for selecting a destination of sending the cleaning liquid.

又、上記排出口11は、途中にロータリ式切換弁16と送液ポンプ17とを設けた排出管18を介して、排液タンク19 Further, the discharge port 11 via the discharge pipe 18 provided with a rotary selector valve 16 and the liquid supply pump 17 on the way, drainage tank 19
に通じさせる事により、洗浄液を含む処理液を排出する処理液排出手段を構成している。 By providing communication to constitute a treatment liquid discharge means for discharging the processing liquid containing the cleaning liquid.

台座部2の上面で、スライドガラス4の両側縁からはみ出した部分に染色用処理液を滴下する滴下手段は、処理液容器収納部20に収納した処理液容器21を、図示しない処理液容器取り出し腕により取り出して、所定の台座部2の上方に迄移動させ、処理液の滴下を行なわせる様に構成している。 In the upper surface of the base portion 2, a dropping means for dropping the dyeing treatment liquid surplus part from both side edges of the slide glass 4, the treatment liquid container 21 accommodating the treatment liquid container holder 20, the eject process liquid container (not shown) removed by the arm moves up above the predetermined pedestal 2 is configured so as to perform the dropping of the processing solution.

上述の様に構成される先発明の免疫反応染色装置により、生体から取り出した組織片の染色処理を行なう場合、染色すべき組織片を貼付したスライドガラス4の一端を壁部6に形成した凹部7に内嵌し、スライドガラス中央の組織片の貼付部分を台座部2の上面に対向させた状態で、このスライドガラス4を、台座部2の端部に設けた土手状部3の上縁と支持板部9の上面とで支持する。 The immunoreactive staining apparatus configured destination invention as described above, when performing the dyeing treatment of tissue pieces taken from a living body, and the one end of the slide glass 4 attached tissue fragments to be dyed is formed on the wall portion 6 recess was fitted to 7, the sticking portion of the slide glass central piece of tissue while being opposed to the upper surface of the base portion 2, the slide glass 4, the upper edge of the bank-shaped portion 3 provided on an end portion of the pedestal 2 It is supported by the upper surface of the support plate 9 and. この状態で、スライドガラス4の下面と台座部2の上面との間には、土手状部3から離れるに従って次第に広くなる、くさび状の薄い隙間が形成される。 In this state, between the lower surface and the upper surface of the base portion 2 of the slide glass 4 is progressively wider with distance from the bank-shaped portion 3, the wedge-shaped thin gap is formed.

この様にスライドガラス4を土手状部3と支持板部9 Bank-like portion 3 slides 4 Thus the support plate 9
とに掛け渡す様に載置したならば、滴下手段を構成する処理液容器取り出し腕により処理液容器21を、処理液と接触させるべき組織片を貼着したスライドガラス4の上方に移動させて、上記処理液容器21内に貯溜してある処理液を、スライドガラス4の側縁から側方に露出した台座部2の上面に滴下する。 Once placed as to bridge the bets, the processing liquid container 21 by the treatment liquid container retrieving arm constituting a dropping means, is moved over the glass slide 4 which is stuck a piece of tissue to be contacted with the treatment liquid It is added dropwise processing solution which had been accumulated in the processing solution container 21, the upper surface of the base portion 2 exposed laterally from the side edge of the slide glass 4.

台座部2に被さり、土手状部3の上縁と支持板部9の上面との間に掛け渡される様に支持されたスライドガラス4の下面と台座部2の上面との間には、上述の様に、 It overlies the base portion 2, between the supported lower surface and the upper surface of the base portion 2 of the slide glass 4 as to be stretched between the upper edge of the bank-like portion 3 and the upper surface of the support plate 9, above as of,
土手状部3から離れるに従って次第に広くなり、くさび状の薄い隙間が形成されている為、台座部2の上面に滴下された処理液は、毛細管現象によって、この隙間の内側に行き亙り、スライドガラス4の下面に貼付された組織片と接触する。 Gradually widens with distance from the bank-shaped portion 3, since the wedge-shaped thin gap is formed, the treatment liquid that has been dropped on the upper surface of the base portion 2, by capillary action, over go inside this gap, glass slides attached to the lower surface of 4 was in contact with the tissue piece.

この様にして、スライドガラス4の下面に貼付した組織片と処理液とを、所定時間接触させたならば、排出口 In this way, the a piece of tissue was adhered to the lower surface of the slide glass 4 and the treatment liquid, if contacted predetermined time, the discharge port
11と排液タンク19とが互いに連通する状態にロータリ式切換弁16を切り換えると共に、それ迄停止していた処理液排出手段を構成する送液ポンプ17を起動して、土手状部3に近接した位置に開口した排出口11から、排液タンク19に向けて処理液を排出する。 11 and switches the rotary switching valve 16 to the state in which the drainage tank 19 communicate with each other, it until start the liquid feed pump 17 constituting the processing solution discharge means that has been stopped, close to the bank-shaped portion 3 from the discharge port 11 which opens to a position to discharge the treated liquid toward the drainage tank 19.

処理液の排出完了後、処理液排出手段を構成する送液ポンプ17を停止させ、代って吐出口10と洗浄液貯溜タンク15とが互いに連通する状態にロータリ式切換弁12を切り換えると共に、それ迄停止していた洗浄液供給手段を構成する送液ポンプ13を所定時間だけ運転して、土手状部3に近接して設けた吐出口10から洗浄液を吐出する。 After completion discharge of the process liquid, the liquid feed pump 17 constituting the processing solution discharge unit is stopped, switches the rotary switching valve 12 in a state where a discharge port 10 and the washing liquid storage tank 15 in place are communicated with each other, it until the liquid feed pump 13 constituting the cleaning liquid supply means that has been stopped by operating a predetermined time, and discharges the cleaning liquid from the discharge ports 10 provided in proximity to the bank-shaped portion 3.

吐出口10から吐出した洗浄液は、毛細管現象によってスライドガラス4の下面と台座部2の上面との間の隙間に行き亙り、スライドガラス4の下面に貼付された組織片に付着した染色処理液の洗浄処理を行なう。 Cleaning liquid discharged from the discharge port 10, over go into the gap between the lower surface and the upper surface of the base portion 2 of the slide glass 4 by the capillary phenomenon, the dyeing treatment liquid attached to the tissue pieces affixed to the lower surface of the slide glass 4 carry out the cleaning process.

所定時間の洗浄処理を終了したならば、再び処理液排出手段を構成する送液ポンプ17を起動して、スライドガラス4の下面と台座部2の上面との間の隙間内の洗浄液を排出した後、次の染色処理液の滴下を行なう。 Once completed the cleaning process for a predetermined time, to start the liquid feed pump 17 constituting the processing solution discharge unit again, and discharged cleaning liquid in the gap between the lower surface and the upper surface of the base portion 2 of the slide glass 4 after performs dropping of the next dyeing treatment liquid.

以下、上述の作業を繰り返し行なって、所定の染色処理を行なう。 Hereinafter, repeatedly performed operations described above, it performs a predetermined dyeing treatment.

(発明が解決しようとする課題) ところが、上述の様に構成され作用する、先発明に係る免疫反応染色装置の場合、依然として次に述べる様な問題が存在する。 (Problems to be Solved) However, it configured to act as described above, when the immune response dyeing apparatus according to the prior invention, there is still described below such problems.

先ず第一に、ロータリ式切換弁12、16を設けた配管系統は複雑で、製作費が嵩む事が避けられない。 First of all, the piping system provided with a rotary switching valve 12, 16 is complex, it is inevitable that the production costs increase.

又、洗浄液を給排する為の吐出口10と排出口11とが、 Further, the discharge port 10 for supplying and discharging a cleaning liquid and an outlet 11,
染色ブロック1の下側に開口している為、この染色ブロック1を使用して、手作業により各種染色処理を行なおうとした場合にも、これを行なう事が出来ない。 Because you are open to the lower side of the dyeing block 1, use this staining block 1, even when the attempt is made various dyeing processing by hand, not be able to do this.

本発明の染色装置と染色用トレーは、上述の様な不都合を何れも解消するものである。 Dyeing apparatus and dyeing tray of the present invention is to eliminate any inconvenience such as described above.

(課題を解決する為の手段) 本発明の染色装置と染色用トレーとの内、染色用トレーは、スライドガラスの下面に貼付した組織片と対向する台座部と、この台座部の一端に形成された浅い凹部と、上記台座部の他端側に設けられた、上端縁の高さが台座部上面の高さよりも僅かに高い支持部と、上記台座部の一端部上面で、上記凹部の一方の側に開口した吐出口と、同じく上記台座部の上面で、上記凹部の他方の側に開口した排出口とを有し、一端部上面に、上記吐出口に通じる給液口と、上記排出口に通じる排液口とを、互いに一定の間隔を開けて開口させる事で構成されている。 Among the dyeing apparatus and dyeing tray of the present invention (means for solving the problem), staining tray, a pedestal portion facing the tissue piece was attached to the lower surface of the slide glass, formed at one end of the pedestal portion shallow recess which is, provided on the other side of the base portion, and a slightly higher support portion than the height of the pedestal upper surface height of the upper edge, at one end the upper surface of the base portion, of the recess a discharge port opened to one side, also in the upper surface of the base portion, and a discharge port opened in the other side of the recess, on one end top surface, a liquid supply ports communicating with said discharge port, said a drain port communicating with the discharge port, and a thing to be opened at regular intervals from each other.

そして、本発明の染色装置は、上述の様に構成され、 The dyeing apparatus of the present invention is constructed as described above,
基台の所定位置に保持された染色用トレーと、この染色用トレーの上面に設けられた凹部に、染色用の処理液を滴下する為の滴下手段と、この滴下手段を上記凹部の上方に移動させる第一の移動手段と、上記染色用トレーの一端部上面に開口した給液口と排液口との間隔と同じ間隔で配置され、下端部をそれぞれ上記給液口又は排液口に接続自在とされた給液管及び排液管を有し、給液管を給液手段に、排液管を排液手段に、それぞれ接続した洗浄手段と、この洗浄手段を構成する上記給液管と排液管とを、上記給液口と排液口との上方位置に移動させると共に、この上方位置で昇降させる第二の移動手段とから構成されている。 A staining tray which is held in place of the base, the recess provided on the upper surface of the dyeing tray, a dropping means for dropping the treatment liquid for dyeing, the dropping means above the recess a first moving means for moving the, are arranged at the same intervals as those of the liquid supply ports which is open at one end the upper surface of the dyeing tray and drain port, a lower end portion, each said liquid supply port or drain port has freely connected and have been the liquid supply pipe and drain pipe, a liquid supply pipe into liquid supply means, the draining means drain pipe, a wash means connected respectively, the liquid supply constituting the cleaning means a tube and drainage tube, is moved to the upper position of the liquid supply port and drain port, and a second moving means for lifting at the upper position.

(作用) 上述の様に構成される本発明の染色装置と染色用トレーとを使用して、スライドガラスに貼付された組織片等の試料の染色処理を行なう場合の作用は、次の通りである。 (Working) using a staining tray and above dyeing apparatus of the present invention constructed as the action in the case of performing dyeing samples of tissue pieces or the like which is attached to a slide glass, as follows is there.

染色処理すべき試料を下面に貼付したスライドガラスは、その一端部下面を台座部の一端部上面に当接させ、 Glass slide affixed stained to be treated sample on the lower surface is brought into contact with one end lower surface on one end top surface of the base portion,
他端部寄り下面を支持部の上縁に当接させる事で、上記台座部の上方に支持する。 By are brought into contact with the other end closer underside on the upper edge of the support portion is supported above the pedestal.

この状態で台座部の一端部上面に形成された凹部は、 Recess formed in one end portion upper face of the pedestal portion in this state,
その一部がスライドガラスの一端縁から露出し、台座部の上面とスライドガラスの下面との間には、上記凹部から離れるに従って次第に広くなる、くさび状の隙間が形成される。 Partially exposed from the one edge of the slide glass, between the lower surface of the upper surface and the slide glass pedestal gradually becomes wider as the distance from the recess, the wedge-shaped gap is formed.

そこで、第一の移動手段により、上記凹部の上方に滴下手段を移動させると共に、この滴下手段により染色用の処理液を、上記凹部に滴下する。 Therefore, the first moving means moves the dropping means above the recess, the processing liquid for dyeing by the dropwise addition means, is added dropwise to the recess. 滴下された処理液は、毛細管現象により上記くさび状の隙間に広がり、スライドガラスの下面で、この隙間に対向する部分に貼付された試料を染色処理する。 The dropped treating liquid spreads to the wedge-shaped gap by capillary action, the lower surface of the glass slide, stained handle affixed sample in a portion opposed to the gap.

染色用の処理液による、所定時間の染色処理を終了したならば、洗浄手段により、上記隙間に存在する処理液を洗い流す。 With a processing solution for dyeing, if you complete the dyeing process of a predetermined time, the cleaning means, washing out the processing liquid present in the gap. 尚、この洗い流しによる洗浄処理作業に先立ち、第二の移動手段により、上記洗浄手段を構成する給液管の端部を給液口に、排液管の端部を排液口に、それぞれ接続しておく。 Incidentally, prior to the cleaning process operation by the rinse, the second moving means, the end of the liquid supply pipe constituting the cleaning means to the liquid supply port, the end of the drain pipe to drain port, respectively connected keep.

洗い流し作業は、上記洗浄手段を構成する給液手段を作動させる事により、給液管を通じて洗浄液を上記隙間に送り込んでから、所定時間経過後に、排液手段によりこの洗浄液を、排液管を通じ排出する事で行なう。 Rinse operation, by actuating the liquid supply means constituting the cleaning means, a cleaning liquid from by feeding into the gap through the liquid supply pipe, after a predetermined time has elapsed, the washing solution by drainage means, discharged through the drain pipe to that carried out at.

所定の洗浄処理作業を行なった後に於いては、再び滴下手段により染色用の処理液を、前記凹部に滴下し、この処理液を台座部の上面とスライドガラスの下面との間の隙間に進入させて、スライドガラス下面に貼付された試料の染色処理を行なう。 Is In the after a predetermined cleaning work, the treatment liquid for dyeing again by dropping means is dropped in the recess, enters the processing solution to the gap between the lower surface of the upper surface and the slide glass pedestal It is allowed to, perform dyeing process affixed sample on the slide glass underside.

以下、上述の作業を、所定回数繰り返し行なう事で、 Hereinafter, the operations described above, by performing a predetermined number of iterations,
所定の染色処理を行なう。 It performs a predetermined staining process.

更に、本発明の染色用トレーは、上述の様に、染色装置に組み込んで、自動的に試料の染色処理を行なう他、 Furthermore, dyeing tray of the present invention, as described above, incorporated into dyeing apparatus, automatic addition performing dyeing process of the sample,
手作業による染色を行なう場合にも使用する事が出来る。 It can be also be used in the case of the staining by hand.

但し、この場合は、凹部への処理液の滴下や給液口への洗浄液の送り込み、或は排液口からの洗浄液の取り出しは、スポイトやピペット等を用いて行なう。 However, in this case, delivery of the cleaning liquid into the dripping and the liquid supply mouth of the processing liquid to the recess, or taken out of the cleaning liquid from the drain port is performed by using a dropper or pipette.

(実施例) 次に、図示の実施例を説明しつつ、本発明を更に詳しく説明する。 (Example) Next, while describing the embodiment shown, the present invention will be described in more detail.

第1〜9図は本発明の染色装置と染色用トレーの実施例を示しており、第1図は染色用トレーの斜視図、第2 First to ninth figure shows an example of the dyeing tray and dyeing apparatus of the present invention, FIG. 1 is a perspective view of a staining tray, a second
図は同じく平面図、第3図は同じく底面図、第4図は第2図のA−A断面図、第5図は同B−B断面図、第6図は第5図のC部拡大断面図、第7図は第1図のD−D断面図、第8図は第7図のE部拡大断面図、第9図は洗浄手段の正面図である。 Figure plan view of the same, FIG. 3 is also a bottom view, FIG. 4 is a sectional view taken along A-A of FIG. 2, FIG. 5 is the sectional view taken along line B-B, Fig. 6 is an enlarged C part of FIG. 5 sectional view, FIG. 7 is D-D sectional view of FIG. 1, FIG. 8 is E enlarged sectional view of FIG. 7, FIG. 9 is a front view of the cleaning means.

先発明に於ける染色ブロックに相当する、本発明の染色用トレー22は、繊維強化プラスチック等により一体に造られている。 Corresponds to in dyeing block above invention, the dyeing tray 22 of the present invention are made together by fiber-reinforced plastic or the like. 全体を矩形とされた、この染色用トレー Whole is rectangular, the dyeing tray
22の四周は、壁部23により囲まれており、この壁部23の内側に、スライドガラス4(第10〜11図参照)の下部に貼付した組織片と対向する、複数の(図示の実施例の場合、5個の)台座部24、24を設け、染色用トレー22の下面で、各台座部24、24と整合する部分は、凹部31、31としている。 Four sides 22 is surrounded by a wall portion 23, inside the wall portion 23, facing the tissue piece was attached to the bottom of the slide glass 4 (see 10-11 Figure), the implementation of a plurality of (illustrated for example, five) pedestal portions 24 provided on the lower surface of the dyeing tray 22, portions aligned with the respective pedestal portions 24, 24 is in the recess 31.

各台座部24、24の一端(第2図の下端)には、それぞれ浅い摺鉢状の凹部25、25が、各台座部24、24の他端側には、各台座部24、24と間隔を開けて、立壁状の支持部 At one end (lower end of FIG. 2) of the pedestal portion 24, 24, shallow sliding pot-shaped recess 25, 25 respectively, to the other end of the base portion 24, 24, each pedestal 24, 24 spaced, vertical wall-shaped support portion
26、26が、それぞれ設けられている。 26, 26, are provided. 尚、上記各支持部 Incidentally, each of the support portions
26、26の上端縁の高さは、それぞれ台座部24、24の上面の高さよりも僅かに高くしている。 The upper end height of the edge of 26 and 26 is slightly greater than the height of the upper surface of each pedestal portion 24, 24.

上記各台座部24、24の一端部上面で、上記各凹部25、 Said one end portion upper face of the pedestal portion 24, 24, the respective recesses 25,
25の一方の側(第2図の右側)には吐出口27、27を、同じく上記台座部24、24の一端部上面で、上記各凹部25、 Discharge ports 27, 27 on one side of the 25 (right of FIG. 2), also at one end an upper surface of the base portion 24 and 24, above each recess 25,
25の他方の側(第2図の左側)には排出口28、28を、それぞれ開口させている。 An outlet 28, 28 to 25 the other side (on the left of FIG. 2), respectively are opened.

又、前記壁部23の一部で、染色用トレー22の一端縁(第2図の下端縁)に存在する部分の上面には、上記吐出口27、27に通じる給液口29、29と、上記排出口28、28 Further, a part of the wall portion 23, the upper surface of the portion present at one end edge of the dyeing tray 22 (the lower edge of FIG. 2) is provided with liquid supply ports 29, 29 communicating with the discharge port 27 , and the discharge port 28, 28
に通じる排液口30、30とを、互いに一定の間隔dを開けた状態で開口させている。 A drain port 30, 30 leading to, and is opened in a state in which at regular intervals d from each other.

尚、図示の実施例に於いては、隣り合う台座部24、24 Note that In the embodiment shown, the adjacent pedestal portions 24, 24
の間に立壁32、32を形成しているが、隣り合う立壁32、 Although forming a vertical wall 32, 32 between the upright wall adjacent 32,
32同士の間隔D、並びに両端部に存在する立壁32、32と染色用トレー22の周縁部の壁部23との間隔Dは、染色処理すべき組織片等の試料を貼付したスライドガラス4の幅w(第10図)よりも少しだけ大きく(D>w)している。 32 distance D between, as well as the distance D between the wall portion 23 of the peripheral portion of the vertical wall 32 and the dyeing tray 22 existing in the both ends, the slide glass 4 was stuck sample of tissue pieces or the like to be dyed It is slightly larger (D> w) than the width w (FIG. 10).

上述の様に構成される染色用トレー22は、下面に形成された凹部31、31に、染色装置を構成する基台(図示せず)の上面に形成された凸部を嵌合させる事で、上記基台上面の所定位置に保持されるが、この染色用トレー22 Staining tray 22 constructed as described above is in the recess 31, 31 formed on the lower surface, a base constituting a dyeing apparatus that fitting the convex portion formed on the upper surface (not shown) , are held at a predetermined position of the upper base, the dyeing tray 22
の上面に設けられた凹部25、25に、染色用の処理液を滴下する滴下手段は、前述した先発明に係る染色装置と同様に、染色処理液を充填した処理液容器21(第9、12 The recess 25, 25 provided in the upper surface, dropping means for dropping the treatment liquid for dyeing, as in the dyeing device according to the prior invention described above, the process was filled with dyed liquid fluid container 21 (No. 9, 12
図)と、この処理液容器21を所定の染色用トレー22の上方に迄移動させ、処理液を滴下させる、第一の移動手段である処理液容器取り出し腕とから構成されている。 And FIG.), The processing liquid container 21 is moved up above the predetermined dyeing tray 22, thereby dropping the treatment liquid, and a treatment liquid container retrieving arm is the first moving means.

尚、処理液容器21は、上端に設けた釦41を下方に押す事により、吐出管42を通じて、内部に貯溜した処理液を吐出自在としたものであり、上記釦41を下方に押圧自在とする為、第一のモータ43により回転駆動される第一の螺子杆44に螺合した第一のナット片45に押圧片46の基端部を固定し、この押圧片46の先端部を、上記釦41の上面に対向させている。 Note that the processing liquid container 21, by pressing the button 41 provided on the upper end downwards, through the discharge pipe 42 is obtained by freely discharge the processing liquid that has accumulated in the interior, freely pressing the button 41 downwardly to order, a base end portion of the pressing piece 46 to the first nut piece 45 screwed to the first screw rod 44 is rotationally driven by the first motor 43 is fixed, the distal end portion of the pressing piece 46, It is made to face the upper surface of the button 41.

又、各台座部24、24の上面とスライドガラス4、4の下面との間に送り込まれた染色用の処理液を洗い流す為の洗浄手段は、第9図に示す様に、給液管33及び排液管 Further, the cleaning means for washing away the treatment solution for staining was fed between the upper and lower surfaces of the slide glass 4,4 for each pedestal portion 24, 24 as shown in FIG. 9, the liquid supply pipe 33 and the drainage tube
34を有し、給液管33を給液手段である図示しない洗浄液容器に、排液管34を図示しない排液手段に、それぞれ接続している。 Has 34, the liquid supply pipe 33 to the cleaning liquid container (not shown) which is liquid supply means, the draining means (not shown) drainage tubes 34 are connected, respectively. 尚、上記給液管33と排液管34との下端開口は、前記染色用トレー22の一端部上面に開口した給液口 Incidentally, the lower end opening of the drain pipe 34 and the liquid feed line 33, liquid supply ports which is open at one end an upper surface of the dyeing tray 22
29と排液口30との間隔dと同じ間隔dで配置されている。 They are arranged at the same distance d as the distance d between 29 and drain port 30.

更に詳しく説明すると、上記給液管33と排液管34とは、前記処理液容器取り出し腕と同期して水平方向に移動する部分に設けられた、第二の移動手段である単一の昇降ブラケット36に対して若干の昇降を自在として支持されており、それぞれ圧縮ばね37、37の弾力によって、 In more detail, the above liquid supply pipe 33 and drain pipe 34, provided in the moving parts in the horizontal direction in synchronization with the processing liquid container retrieving arm, single lifting a second moving means is supported as freely a slight elevation relative to the bracket 36 by a respective resilient compression spring 37,
下方に向かう弾力を付与されている。 The elasticity directed downward have been granted. 上記昇降ブラケット36に固定の第二のナット片38は、第二のモータ39により正転、逆転自在な、第二の螺子杆40と螺合しており、 The lift bracket 36 on the fixed second nut piece 38, forward by a second motor 39, freely reverse, and screwed into the second screw rod 40,
上記第二のモータ39により、上記昇降ブラケット36を昇降駆動自在としている。 By the second motor 39, and a vertically movable driving the lift bracket 36.

給液管33の上端部は、途中に送りポンプを設けた、図示しないチューブにより、図示しない洗浄容器に通じさせており、上記送りポンプの運転に伴ない、前記給液口 The upper end of the liquid supply pipe 33, provided with a feed pump in the middle, by a not-shown tube, and vented to the cleaning container (not shown), In conjunction with operation of the feed pump, the liquid supply port
29に洗浄液を送り込める様にしている。 And in the manner Okurikomeru a cleaning liquid to 29. 一方、排液管34 Meanwhile, drain pipe 34
の上端部は、やはり図示しないチューブにより、排液容器等の、図示しない排液手段に通じさせている。 The upper end of the also by not-shown tube, such as a drainage container is vented to the draining means (not shown).

上述の様に構成される本発明の染色装置と染色用トレーとを使用して、スライドガラスに貼付された試料の染色処理を行なう場合、次の様にして行なう。 Using a dyeing apparatus and dyeing tray of the present invention constructed as described above, when performing the dyeing process of the patch is a sample on a slide glass, performed in the following manner.

染色処理すべき試料を下面に貼付したスライドガラスは、その一端部下面を各台座部24、24の一端部(第2図の下端部)上面に当接させ、他端部寄り下面を、支持部 Glass slide affixed stained to be treated sample on the lower surface is brought into contact with one end lower surface to the upper surface (lower end of FIG. 2) one end of each pedestal 24, 24, the other end closer underside, supporting part
26、26の上縁に当接させる事で、上記各台座部24、24の上方に支持する。 By abut the upper edge of 26 and 26, supported above the respective base portions 24, 24.

この状態で各台座部24、24の一端部上面に形成された凹部25、25は、その一部がスライドガラスの一端縁から露出し、各台座部24、24の上面とスライドガラスの下面との間には、上記凹部25、25から離れるに従って次第に広くなる、くさび状の隙間が形成される。 Recesses 25 and 25 formed at one end portion upper face of the pedestal portion 24, 24 in this state is partially exposed from the one edge of the slide glass, and the lower surface of the upper surface and the slide glass of each pedestal 24, 24 between gradually becomes wider as the distance from the recess 25, the wedge-shaped gap is formed. 又、各スライドガラスの両側縁は、染色用トレー22の周縁部に形成した壁部23の内側縁、或は隣り合う台座部24、24の間に形成した立壁32、32の側縁に近接した状態となる。 Further, both side edges of each slide is proximate to the inner edge, or side edges of the vertical wall 32 formed between the base portion 24, 24 adjacent the wall portion 23 formed on the periphery of the staining tray 22 the state.

上述の様にスライドガラスをセットした状態で、滴下手段を構成する処理液容器21により染色用の処理液を、 At setting the slide glass as described above, the processing liquid for dyeing by the processing liquid container 21 constituting a dropping means,
上記各台座部24、24の一端に形成した凹部25、25に滴下すれば、毛細管現象によりこの処理液が、上記スライドガラスの下面と台座部24、24の上面との間に存在するくさび状の隙間に広がり、スライドガラスの下面で、この隙間に対向する部分に貼付された試料を染色処理する。 If dropped into the recess 25 formed in one end of the respective pedestals 24 and 24, the treatment liquid by capillary action, like a wedge that exists between the upper surface of the lower surface and the base portion 24, 24 of the slide glass spread the gap at the lower surface of the glass slide, stained treating a sample affixed to a portion facing the gap.
この際、スライドガラスの両側縁が立壁32、32の側面(或は壁部23の内側面)に近接している為、上記隙間内に送り込まれた処理液が、短い時間の間に蒸発し、減少する事が防止される。 In this case, since the both side edges of the slide glass is close to vertical wall 32, 32 side of the (or the inner surface of the wall portion 23), processing liquid fed into the said gap, evaporated for a short time , that decrease can be prevented.

染色処理液の滴下作業を行なう場合には、前記第一のモータ43により第一の螺子杆44を回転させ、第一のナット片45、押圧片46を介して、処理液容器21の釦41を下方に押圧し、吐出管42から処理液を吐出させる。 When performing the dropping operations of dyeing liquid, said by the first motor 43 to rotate the first screw rod 44, the first nut member 45 through the pressing piece 46, button 41 of the processing liquid container 21 pressing the downwardly to eject the processing liquid from the discharge pipe 42.

染色用の処理液による、所定時間の染色処理を終了したならば、洗浄手段により、上記隙間に存在する処理液を洗い流すが、この洗い流しによる洗浄処理作業に先立ち、上記洗浄手段を構成する給液管33の下端部を給液口 With a processing solution for dyeing, if you complete the dyeing process of a predetermined time, the cleaning means, liquid supply wash away the processing solution present in the gap, but that prior to the cleaning process operation by this wash, constituting the cleaning means the lower end of the tube 33 liquid supply ports
29に、排液管34の下端部を排液口30に、それぞれ接続しておく。 29, the lower end of the drain pipe 34 to drain port 30, keep connected.

即ち、図示しない二次元駆動機構により、第9図に示した洗浄手段の給液管33及び排液管34を、所定の給液口 That is, the two-dimensional driving mechanism (not shown), the liquid supply pipe 33 and drain pipe 34 of the washing means shown in FIG. 9, a predetermined liquid supply ports
29及び排液口30の上方に移動させたならば、第二のモータ39により第二の螺子杆40を回転させる事で、それ迄上昇していた昇降ブラケット36を下降させ、上記給液管33 Once it moved 29 and above the liquid discharge port 30, the second motor 39 by rotating the second screw rod 40 lowers the lift bracket 36 which has been increased to it, the liquid supply pipe 33
及び排液管34を、上記所定の給液口29及び排液口30に押し付ける。 And drainage tubes 34, pressed to the predetermined liquid supply ports 29 and drain port 30.

上記給液管33及び排液管34は、それぞれ昇降ブラケット36に対して昇降自在とされると共に、圧縮ばね37、37 The liquid supply pipe 33 and drain pipe 34, while being freely move in the vertical direction with respect to each lift bracket 36, compression springs 37 and 37
の弾力により下方に押圧されており、且つ、各管33、34 Of it is pressed downward by the elastic force, and, each tube 33, 34
の下端開口周縁部には、ゴムリング等のシール材が装着されている為、昇降ブラケット36の下降に伴ない、給液管33及び排液管34を、上記所定の給液口29及び排液口30 Of the lower end opening peripheral edge, since the sealing member of rubber ring or the like is mounted, not accompanied the descent of the lift bracket 36, the liquid supply pipe 33 and drain pipe 34, the predetermined liquid supply ports 29 and discharge liquid port 30
に押し付ける事により、洗浄手段を構成する給液管33の下端部と給液口29とが、排液管34の下端部と排液口30とが、それぞれ液密に接続される。 By pressing the lower end of the liquid supply pipe 33 constituting the cleaning means and the liquid supply ports 29, the lower end of the drain pipe 34 and the drain port 30 are connected to respective liquid-tight.

洗浄処理作業を行なう場合には、図示しないチューブの途中に設けた送りポンプを運転する事により、やはり図示しない洗浄液タンク内に貯溜された洗浄液を、給液管33、給液口29を通じて、台座部24の上面とスライドガラスの下面との間に存在する隙間に送り込む。 When performing a cleaning process operation, by operating the feed pump provided in the middle of the tube, not shown, also the reservoir cleaning liquid to the cleaning liquid tank (not shown), the liquid supply pipe 33, through the liquid supply ports 29, the pedestal It fed into the gap that exists between the upper and lower surfaces of the slide glass parts 24.

この様にして上記隙間に洗浄液を送り込んでから所定時間経過後に、排液手段に通じる、図示しない別のポンプを運転する事により、この洗浄液を、排液口30、排液管34を通じ排出する事で、洗浄処理作業を行なう。 Such a manner of by feeding a cleaning liquid to the gap after a predetermined time has elapsed, leading to the draining means, by operating the separate pump (not shown), the cleaning liquid drainage port 30, is discharged through the drain pipe 34 thing in, carry out the cleaning process work.

所定の洗浄処理作業を行なった後に於いては、再び滴下手段を構成する処理液容器21により染色用の処理液を、前記台座部24の一端に形成した凹部25に滴下し、この処理液を台座部24の上面とスライドガラスの下面との間の隙間に進入させて、スライドガラス下面に貼付された試料の染色処理を行なう。 Is In the after a predetermined cleaning operations, dropwise treatment liquid for dyeing, in a recess 25 formed at one end of the base portion 24 by the processing liquid container 21 constituting a dropping unit again, the processing solution by entering into the gap between the upper and lower surfaces of the slide glass of the pedestal portion 24, it performs the dyeing process of the patch is a sample on a slide glass underside.

以下、上述の作業を、所定回数繰り返し行なう事で、 Hereinafter, the operations described above, by performing a predetermined number of iterations,
所定の染色処理を行なう。 It performs a predetermined staining process.

更に、本発明の染色用トレーは、上述の様に、染色装置に組み込んで、自動的に試料の染色処理を行なう他、 Furthermore, dyeing tray of the present invention, as described above, incorporated into dyeing apparatus, automatic addition performing dyeing process of the sample,
手作業による染色を行なう場合にも使用する事が出来る。 It can be also be used in the case of the staining by hand.

但し、この場合は、凹部25、25への処理液の滴下や給液口29、29への洗浄液の送り込み、或は排液口30、30からの洗浄液の取り出しは、スポイトやピペット等を用いて行なう。 However, in this case, delivery of the cleaning liquid into the dripping or liquid supply ports 29, 29 of the processing liquid into the recess 25, 25, or taken out of the cleaning liquid from the drain port 30 and 30, using a dropper or pipette and conduct.

この場合に於いて、本発明の染色用トレー22は、上面に透明樹脂板等の蓋を被せる事が可能である為、十分な保湿効果が得られ、染色作業が長時間に及ぶ場合にも、 In this case, the dyeing tray 22 of the present invention, since it is possible to put the lid of the transparent resin plate or the like on the upper surface, sufficient moisturizing effect can be obtained even if the dyeing work spans a long period of time ,
試料の乾燥防止を確実に図る事が出来る。 Can be achieved with certainty prevent drying of the sample. 更に、染色用トレー22を重ね合わせておく事も出来る為、多数の試料の染色処理を行なう場合にも、作業に要する面積が狭くて済む。 Furthermore, since it is also possible to have superimposed staining tray 22, even when performing the dyeing treatment of a large number of samples, it requires only a narrow area required for the work.

(発明の効果) 本発明の染色装置と染色用トレーは、以上に述べた通り構成され作用する為、少ない処理液で、免疫反応を観察する為の組織片等の試料の染色処理を自動的に行なう事が出来、免疫反応観察等、染色処理に要する費用の低減化、省力化に寄与する効果が大きいと言った。 Dyeing apparatus and dyeing tray of the present invention (the effect of the invention), to act configured as described above, with less processing solution, automatically staining treatment of the sample of the tissue pieces or the like for observing the immune response can be carried out, the immune response observation or the like, reduction of the cost required for the dyeing process, said that a large effect of contributing to labor saving. 先発明の有する効果をそのままにして、染色作業の開始時に空気を追い出す作業を行なう必要が不要となり、染色作業の準備行程を簡略化する事が出来るだけなく、ロータリ式切換弁を設けた複雑な配管系統が不要となる為、装置製作費の低廉化を図れる。 And the effects possessed by the prior invention as it is, dyeing at the start of the work necessary to perform the task of expelling the air is not necessary, not only the provisional step of dyeing work can be simplified, complex in which a rotary selector valve since the piping system is not required, thereby the low cost of the device manufacturing cost.

更に、本発明の染色用トレーは、染色装置に組み込んで、自動染色を行なう場合だけでなく、手作業により各種染色処理を行なう場合にも使用出来る為、多種の染色作業を行なう様な研究所等に於いても、用具管理の簡略化を図る事が可能となる。 In addition, staining for the tray of the present invention, incorporated into the dyeing apparatus, not only when performing automated staining, because that can also be used to perform various types of dyeing processing by hand, such laboratories perform a variety of staining work also in the etc., it is possible to simplify the tool management.

【図面の簡単な説明】 BRIEF DESCRIPTION OF THE DRAWINGS

第1〜9図は本発明の染色装置と染色用トレーの実施例を示しており、第1図は染色用トレーの斜視図、第2図は同じく平面図、第3図は同じく底面図、第4図は第2 First to ninth figure shows an example of the dyeing tray and dyeing apparatus of the present invention, FIG. 1 is a perspective view of a staining tray, Figure 2 is plan view of the same, FIG. 3 is also a bottom view, Figure 4 is a second
図のA−A断面図、第5図は同B−B断面図、第6図は第5図のC部拡大断面図、第7図は第1図のD−D断面図、第8図は第7図のE部拡大断面図、第9図は洗浄手段の正面図、第10〜12図は先発明に係る染色装置を示しており、第10図は滴下手段を省略して示す斜視図、第11 A-A sectional view of FIG, FIG. 5 is the sectional view taken along line B-B, Fig. 6 is C part enlarged sectional view of FIG. 5, FIG. 7 is D-D sectional view of FIG. 1, FIG. 8 E enlarged sectional view of FIG. 7 is a front view of FIG. 9 is cleaning means, 10-12 drawing shows a dyeing apparatus according to the prior invention, perspective Fig. 10 showing by omitting the dropped means FIG, 11
図は第10図のF−F断面図、第12図は滴下手段を構成する処理液容器収納部の斜視図である。 Figure F-F sectional view of FIG. 10, FIG. 12 is a perspective view of the processing liquid container holder which constitutes the dropping means. 1:染色ブロック、2:台座部、3:土手状部、4:スライドガラス、5:排液溝、6:壁部、7:凹部、8:排液溝、9:支持板部、10:吐出口、11:排出口、12:ロータリ式切換弁、13: 1: Staining Block, 2: pedestal, 3: bank-shaped portion, 4: slide glass, 5: drainage ditch, 6: wall, 7: recess, 8: drainage ditch, 9: support plate, 10: discharge port, 11: outlet, 12: rotary switching valve, 13:
送液ポンプ、14:送り込み管、15:洗浄液貯溜タンク、1 Liquid transfer pump, 14: feed pipe, 15: cleaning liquid storage tank, 1
6:ロータリ式切換弁、17:送液ポンプ、18:排出管、19: 6: rotary switching valve, 17: liquid feed pump, 18: exhaust pipe, 19:
排液タンク、20:処理液容器収納部、21:処理液容器、2 Drainage tank, 20: processing liquid container holder, 21: processing liquid vessel, 2
2:染色用トレー、23:壁部、24:台座部、25:凹部、26:支持部、27:吐出口、28:排出口、29:給液口、30:排液口、 2: staining tray, 23: wall portion, 24: pedestal, 25: recess, 26: support, 27: discharge port 28: outlet, 29: liquid supply ports, 30: drain port,
31:凹部、32:立壁、33:給液管、34:排液管、36:昇降ブラケット、37:圧縮ばね、38:第二のナット片、39:第二のモータ、40:第二の螺子杆、41:釦、42:吐出管、43:第一のモータ、44:第一の螺子杆、45:第一のナット片、4 31: recess, 32: vertical wall, 33: liquid supply tube, 34: drain pipe, 36: lift bracket, 37: compression spring, 38: a second nut pieces, 39: second motor, 40: second screw rod, 41: button, 42: discharge pipe, 43: first motor, 44: first screw rod, 45: first nut pieces, 4
6:押圧片。 6: pressing piece.

Claims (4)

    (57)【特許請求の範囲】 (57) [the claims]
  1. 【請求項1】スライドガラスの下面に貼付した組織片と対向する台座部と、この台座部の一端に形成された浅い凹部と、上記台座部の他端側に設けられた、上端縁の高さが台座部上面の高さよりも僅かに高い支持部と、上記台座部の一端部上面で、上記凹部の一方の側に開口した吐出口と、同じく上記台座部の上面で、上記凹部の他方の側に開口した排出口とを有し、一端部上面に、上記吐出口に通じる給液口と、上記排出口に通じる排液口とを、互いに一定の間隔を開けて開口させて成り、基台の所定位置に保持された染色用トレーと、この染色用トレーの上面に設けられた上記凹部に、染色用の処理液を滴下する為の滴下手段と、この滴下手段を上記凹部の上方に移動させる第一の移動手段と、上記染色用トレーの一端部上面に開口した And 1. A tissue section was attached to the lower surface of the slide glass facing the base portion, and a shallow recess formed in one end of the base portion, provided on the other side of the base portion, the upper edge high a slightly higher support portion than the height of Saga pedestal upper surface, at one end the upper surface of the pedestal portion, a discharge port opened to one side of the recess, also in the upper surface of the base portion, the other of the recess side and an open ended outlet, the one end portion upper surface, and the liquid supply ports communicating with said discharge port, and a drain port leading to the discharge port, made by opening at regular intervals from each other, a staining tray which is held in place of the base, in the recess provided on the upper surface of the dyeing tray, a dropping means for dropping the treatment liquid for dyeing, upward the dropping means of the recess a first moving means for moving, the opened one end portion upper surface of the dyeing tray 液口と排液口との間隔と同じ間隔で配置され、下端部をそれぞれ上記給液口又は排液口に接続自在とされた給液管及び排液管を有し、給液管を給液手段に、排液管を排液手段に、それぞれ接続した洗浄手段と、この洗浄手段を構成する上記給液管と排液管とを、上記給液口と排液口との上方位置に移動させると共に、この上方位置で昇降させる第二の移動手段とから成る、染色装置。 Are arranged at the same intervals as those of the liquid inlet and the liquid discharge port, the lower end portion respectively have a liquid supply pipe and drain pipe are freely connected to the liquid supply port or drain port, supplying the liquid supply pipe the liquid means, the drainage means drainage pipes, a cleaning means connected respectively to the said liquid supply tube constituting the cleaning means and drain pipe, the upper position of the liquid supply port and drain port causes moved, comprising a second moving means for lifting at this upper position, dyeing apparatus.
  2. 【請求項2】第一の移動手段と第二の移動手段とが同期して水平方向に移動自在とされている、請求項1に記載の染色装置。 Wherein the first moving means and second moving means are movable in a horizontal direction synchronously, dyeing apparatus according to claim 1.
  3. 【請求項3】スライドガラスの下面に貼付した組織片と対向する台座部と、この台座部の一端に形成された浅い凹部と、上記台座部の他端側に設けられた、上端縁の高さが台座部上面の高さよりも僅かに高い支持部と、上記台座部の一端部上面で、上記凹部の一方の側に開口した吐出口と、同じく上記台座部の上面で、上記凹部の他方の側に開口した排出口とを有し、一端部上面に、上記吐出口に通じる給液口と、上記排出口に通じる排液口とを、互いに一定の間隔を開けて開口させて成る染色用トレー。 3. A piece of tissue was adhered to the lower surface of the slide glass facing the base portion, and a shallow recess formed in one end of the base portion, provided on the other side of the base portion, the upper edge high a slightly higher support portion than the height of Saga pedestal upper surface, at one end the upper surface of the pedestal portion, a discharge port opened to one side of the recess, also in the upper surface of the base portion, the other of the recess and a discharge port that opens to the side of, at one end an upper surface, a liquid supply ports communicating with said discharge port, and a drain port communicating with the discharge port, and by opening at regular intervals from each other staining use tray.
  4. 【請求項4】台座部の両側に、スライドガラスの幅よりも僅かに大きな間隔を開けて立壁を形成した、請求項3 On both sides of 4. A pedestal to form a vertical wall spaced a greater distance slightly larger than the width of the slide glass, claim 3
    に記載の染色用トレー。 Staining tray as set forth in.
JP1246510A 1989-09-25 1989-09-25 Dyeing apparatus and dyeing tray Expired - Lifetime JP2834787B2 (en)

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Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
JP1246510A JP2834787B2 (en) 1989-09-25 1989-09-25 Dyeing apparatus and dyeing tray

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JPH03111035A JPH03111035A (en) 1991-05-10
JP2834787B2 true JP2834787B2 (en) 1998-12-14

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Publication number Priority date Publication date Assignee Title
JP3320444B2 (en) * 1992-04-06 2002-09-03 株式会社千代田製作所 Nozzle cleaning equipment for dyeing equipment
CN110243653A (en) 2012-11-01 2019-09-17 莱卡生物系统墨尔本私人有限公司 Fluid delivery system

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