JPH02227068A - Proliferation of vesicular arbuscular mycorrhiza bacteria - Google Patents
Proliferation of vesicular arbuscular mycorrhiza bacteriaInfo
- Publication number
- JPH02227068A JPH02227068A JP4818489A JP4818489A JPH02227068A JP H02227068 A JPH02227068 A JP H02227068A JP 4818489 A JP4818489 A JP 4818489A JP 4818489 A JP4818489 A JP 4818489A JP H02227068 A JPH02227068 A JP H02227068A
- Authority
- JP
- Japan
- Prior art keywords
- vam
- day
- soil
- bacteria
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 28
- 230000035755 proliferation Effects 0.000 title abstract description 4
- 241000773655 Ambispora fennica Species 0.000 title abstract 2
- 239000002689 soil Substances 0.000 claims abstract description 35
- 230000004069 differentiation Effects 0.000 claims abstract description 6
- 238000004904 shortening Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 19
- 241000233866 Fungi Species 0.000 claims description 16
- 230000001902 propagating effect Effects 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 230000002538 fungal effect Effects 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 34
- 241000235502 Gigaspora margarita Species 0.000 abstract description 10
- 239000003337 fertilizer Substances 0.000 abstract description 9
- 244000124853 Perilla frutescens Species 0.000 abstract description 3
- 230000009105 vegetative growth Effects 0.000 abstract description 2
- 235000010469 Glycine max Nutrition 0.000 abstract 1
- 244000068988 Glycine max Species 0.000 abstract 1
- 235000004348 Perilla frutescens Nutrition 0.000 abstract 1
- 230000001154 acute effect Effects 0.000 abstract 1
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 4
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 4
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 3
- 241000235503 Glomus Species 0.000 description 3
- 235000011941 Tilia x europaea Nutrition 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000004571 lime Substances 0.000 description 3
- 239000000395 magnesium oxide Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- -1 Cas Mg Substances 0.000 description 2
- 235000004347 Perilla Nutrition 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000007952 growth promoter Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 2
- 229920002554 vinyl polymer Polymers 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000023308 Acca Species 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241001424309 Arita Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 235000001602 Digitaria X umfolozi Nutrition 0.000 description 1
- 235000017898 Digitaria ciliaris Nutrition 0.000 description 1
- 235000005476 Digitaria cruciata Nutrition 0.000 description 1
- 235000006830 Digitaria didactyla Nutrition 0.000 description 1
- 235000005804 Digitaria eriantha ssp. eriantha Nutrition 0.000 description 1
- 235000010823 Digitaria sanguinalis Nutrition 0.000 description 1
- 244000025670 Eleusine indica Species 0.000 description 1
- 235000014716 Eleusine indica Nutrition 0.000 description 1
- 241001123595 Entrophospora Species 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 241000235500 Gigaspora Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 241001521786 Racocetra gregaria Species 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000005539 carbonized material Substances 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、のう状体−樹枝状体菌根菌（以下、ＶＡＭ菌
と云う）の効率的な増殖方法に関し、短日植物栽培によ
る共生にＶＡＭ菌を用いることでその増殖を図るように
したものである。Detailed Description of the Invention (Industrial Application Field) The present invention relates to an efficient method for propagating vesicular-arbuscular mycorrhizal fungi (hereinafter referred to as VAM fungi) using short-day plant cultivation. By using VAM bacteria for symbiosis, its proliferation is attempted.
（従来の技術）
植物と微生物との共生関係は古くから知られており、Ｖ
ＡＭ菌（Ｖｅｓｉｃｕｌａｒ ＡｒｂｕｓｃｕｌａｒＭ
ｙｃｏｒｒｈＬｚａ）は多くの植物の根と共生して、そ
の菌糸を根の周囲の土壌中に張りめぐらし、植物の生育
に必要な燐やＣａｓ Ｍｇ、 Ｚｎ等の微量要素を植物
に供給している。また、土壌中の湿度変化や塩類濃度障
害に対する植物の抵抗性を増し、土壌病害の発生を抑制
している。(Conventional technology) The symbiotic relationship between plants and microorganisms has been known for a long time, and V
AM bacteria (Vesicular Arbuscular M
ycorrhLza) coexists with the roots of many plants and spreads its hyphae into the soil around the roots, supplying plants with trace elements such as phosphorus, Cas Mg, and Zn, which are necessary for plant growth. It also increases the resistance of plants to humidity changes and salt concentration disturbances in the soil, and suppresses the occurrence of soil diseases.
しかし、このように有益な働きをするＶＡＭ菌は、その
繁殖を妨げる多量の農薬や肥料を使用することが一般的
となった近年の農耕地には、あまり存在しない、その為
、作物とＶＡＭ菌とを共生させる為には、予め増殖した
ＶＡＭ菌を作物に接種する必要がある。ところが、ＶＡ
Ｍ菌は絶対共生菌の一種といわれており、宿生植物を介
在しない、いわゆる純粋培養による増殖ができないとい
われている。However, in recent years, it has become common to use large amounts of pesticides and fertilizers to prevent VAM bacteria from multiplying, and VAM bacteria, which have such beneficial effects, are rarely present in agricultural land. In order to coexist with bacteria, it is necessary to inoculate crops with VAM bacteria that have grown in advance. However, the V.A.
The M bacterium is said to be a type of obligate symbiotic bacterium, and is said to be incapable of propagation by so-called pure culture without intervening host plants.
しかしてこれらＶＡＭ菌の増殖については、土壌に針葉
樹、広葉樹、農産物の残材等の炭化物に肥料を添加した
炭肥料を施用する方法（特開昭６０−４９７１７号公報
）、天然産または合成品からなる無機及び有機の多孔性
吸着材を含有するＶＡＭ菌用培地を使用する方法（特開
昭６０−２３７９８７号公報）、あるいは、ＶＡＭ菌生
長促進材または、ＶＡＭ菌形酸形成促進材着させた多孔
性の両イオン交換体とを含む培土を使用する方法（特開
昭６３−８７９７３号公報）等が知られている。However, regarding the growth of these VAM bacteria, methods such as applying charcoal fertilizer, which is made by adding fertilizer to carbonized materials such as coniferous trees, broad-leaved trees, and agricultural residues, to the soil (Japanese Patent Application Laid-open No. 60-49717), natural or synthetic fertilizers, (Japanese Unexamined Patent Publication No. 60-237987), or a method using a VAM bacterial culture medium containing an inorganic and organic porous adsorbent consisting of a VAM bacterial growth promoter or a VAM bacterial acid formation promoter. A method of using a soil containing a porous amphoteric ion exchanger (Japanese Unexamined Patent Publication No. 63-87973) is known.
しかしながらかかる方法は、いずれも操作が繁雑であり
、またコスト高である。However, all of these methods require complicated operations and are expensive.
（発明が解決しようとする問題点）
絶対共生菌の一種で、あるＶＡＭ菌を増殖させるために
は、宿主となる植物を必要とする。また、ＶＡＭ菌の接
種層としては、ＶＡＭ菌の共生した根、菌糸、胞子等が
あるが、一般に胞子を用いることが多い、ＶＡＭ菌の生
活環と宿主植物の生活環は一致しており、ＶＡＭ菌の増
殖は植物の生育に支配される。そのため、ＶＡＭ菌生長
促進剤あるいは、ＶＡＭ菌形酸形成促進剤よる増殖が試
みられているが、その方法では増殖に要する期間につい
ては短縮させることができず、現在までのところ、人為
的に給水を停止して植物を枯死させることにより、強制
的に胞子を形成させることが行なわれているが、この方
法では、若干のＶＡＭ菌の増殖が認められているにすぎ
ない、そのため、ＶＡＭ菌を短期間で効率的に増殖する
方法が求められている。(Problems to be Solved by the Invention) VAM is a type of obligate symbiotic bacteria that requires a host plant in order to propagate. In addition, the inoculation layer for VAM fungi includes symbiotic roots, hyphae, spores, etc., but spores are generally used.The life cycle of VAM fungi and the life cycle of the host plant are the same. The growth of VAM bacteria is controlled by the growth of plants. Therefore, attempts have been made to propagate using VAM bacterial growth promoters or VAM bacterial acid formation promoters, but these methods have not been able to shorten the period required for proliferation, and so far, artificial water supply has not been possible. This method has been used to forcibly form spores by stopping the VAM bacteria and causing the plants to wither, but this method only allows a small amount of VAM bacteria to grow. There is a need for a method to efficiently proliferate in a short period of time.
しかしながら現在まで、ＶＡＭ菌を増殖する際に、宿主
植物として短日植物を用い、その栽培期間中の日長時間
を人為的に調節する方法は開発されていない。However, to date, no method has been developed for propagating VAM fungi using short-day plants as host plants and artificially regulating the day length during the cultivation period.
（問題点を解決するための手段）
本発明は上記諸点を考慮し種々検討した結果、ＶＡＭ菌
を含んだ培土で、１日の日長が一定の長さ以下になった
時、花芽分化のみられる短日植物を栽培し、栽培期間中
の日長時間を短縮して、宿主植物の花芽分化を促進する
ことにより、ＶＡＭ菌を短期間で効率的に増殖させるこ
とができることに着目した。(Means for Solving the Problems) As a result of various studies in consideration of the above points, the present invention was developed based on the results of various studies in which flower bud differentiation occurs only when the daily day length falls below a certain length in culture soil containing VAM bacteria. We focused on the fact that VAM fungi can be efficiently propagated in a short period of time by cultivating short-day plants, shortening the day length during the cultivation period, and promoting flower bud differentiation of the host plants.
ＶＡＭ菌としては、ＶＡＭを形成する菌であればいずれ
でもよく、例えば、ギガスポラ（Ｇｉｇａｓｐｏｒａ）
、グＯ？ス（Ｇｌｏｍｏｕｓ）、スクレロシスチス（Ｓ
ｃｌｅｒｏｃｙｓｔｉｓ）、エントロホスポラ（Ｅｎ
ｔｒｏｐｈｏｓｐｏｒａ）、アカウロスポラ（Ａｃｃａ
ｕ １ｏｓｐｏｒａ）属の菌が用いられる。具体的には
、ギガスポラマルガリタ（Ｇｉｇａｓｐｏｒａｍａｒｇ
ａｒｉｔａ）、ギガスポラグレガリア（Ｇｉｇａｓｐｏ
ｒａｇｒｅｇａｒｉａ）及び、グＯ？ス（Ｇｌｏｍｏｕ
ｓ）属があげられる。The VAM bacterium may be any bacterium that forms VAM, such as Gigaspora
, GuO? Glomous, Sclerocystis
clerocystis), Entrophospora (En
trophospora), Acaurospora (Acca
Bacteria of the genus u1ospora) are used. Specifically, Gigaspora margarita (Gigaspora margarita)
arita), Gigaspora gregaria (Gigaspo
ragregaria) and GuO? Glomou
s) The genus can be mentioned.
宿主植物としては、アブラナ科あるいは、アカザ科等の
ＶＡＭ菌が感染しにくい植物以外の短日植物であれば何
れでもよ（、具体的には、シソ、エダマメ、メヒシバ、
トウガラシ、イチゴ、キク、イモ類等が用いられるがこ
れらに限定されるものではない。As a host plant, any short-day plant other than plants that are difficult to be infected by VAM fungi, such as Brassicaceae or Chenopodiaceae, can be used (specifically, perilla, edamame, crabgrass, etc.).
Peppers, strawberries, chrysanthemums, tubers, etc. are used, but are not limited to these.
培土としては、土、砂、轢、腐葉土、又はピートモス等
の有機物、及びそれらの混合物が用いられる。As the culturing soil, organic matter such as soil, sand, road, humus, or peat moss, and mixtures thereof are used.
短日植物の栽培は、温度１５℃〜３５℃、日長時間１４
時間〜１８時間で１〜２力月間栄養生長を行ない、茎葉
部、及び根部の発達をうながす、その後、日長時間を８
時間〜１２時間に調節して人為的に花芽分化を促進させ
、１〜２力月間生植生長させ、栽培を終了する。このよ
うにして、根の周囲にＶＡＭ菌の胞子を大量に含む培土
を得るようにしたものである。Cultivation of short-day plants requires a temperature of 15°C to 35°C and a daylength of 14°C.
Vegetative growth is performed for 1 to 2 months at 18 hours to encourage the development of stems, leaves, and roots, and then the daylength is increased to 8 hours.
The flower bud differentiation is artificially promoted by adjusting the time to 12 hours, and the plants are allowed to grow for 1 to 2 months, and then the cultivation is completed. In this way, a culture soil containing a large amount of VAM fungus spores is obtained around the roots.
このように本発明におけるＶＡＭ菌の増殖法では、宿主
植物の栽培期間を短縮させるために宿主植物とＶＡＭ菌
との共生を促進させることが重要で、その共生速度を促
進させるためにはＶＡＭ菌と宿主植物の根との接触頻度
を高めることが必要である。As described above, in the method for propagating VAM fungi of the present invention, it is important to promote symbiosis between the host plant and the VAM fungus in order to shorten the cultivation period of the host plant. It is necessary to increase the frequency of contact between the plant and the roots of the host plant.
かかる接触頻度を高める方法としては、ＶＡＭ菌を含む
培土を例えば小型容器（容量ｌＯ〜２５０−）に充填し
、発根する根との接触を密にして宿主植物を育苗し、根
とＶＡＭ菌を共生させるようにした後、大型容器に移植
することでより効率的なＶＡＭ菌の増殖を図ることがで
きるものである。As a method to increase the frequency of such contact, for example, fill a small container (capacity 1O~250-) with culture soil containing VAM bacteria, bring up seedlings of host plants in close contact with the roots that will germinate, and connect the roots and VAM bacteria. By allowing VAM bacteria to coexist and then transplanting them into a large container, more efficient growth of VAM bacteria can be achieved.
なお、最初の培土中のＶＡＭ菌の胞子密度は宿主植物の
育成期間によって異なるが、−量的には培土１００ｄ当
たり少なくとも１０個以上、より好ましくは２０個以上
の胞子を含有させることが望ましく、それ以下では増殖
に長期間を要するため効率的ではない。The spore density of the VAM fungus in the initial culture soil varies depending on the growing period of the host plant, but - in terms of quantity, it is desirable to contain at least 10 or more spores, more preferably 20 or more spores per 100 d of culture soil. If it is less than that, it will not be efficient because it will take a long time to proliferate.
以下実施例により本発明を説明するが、これらによって
限定されるものではない。The present invention will be explained below with reference to Examples, but is not limited thereto.
実施例１
キュウリを栽培した跡地から採取したギガスポラ・マル
ガリタの胞子を超音波洗浄器で表面に付着した土壌粒子
等の異物を分離した後、滅菌水で水洗した。ついで直径
９ａｉからなる容量２５０ｍのビニルポットに滅菌処理
した土（粒径２ｎ以下）を充填し、エダマメの種子を２
粒ずつ播種し、第１表に示したようにギガスポラ・マル
ガリタの胞子をポット当たり、５０個、２００個、５０
０個接接種たのち、これらのポットを２５℃±２℃、１
５．０００ルツクス日長時間１６時間の恒温槽内で７週
間育苗した。接種後、１週間目から、１週間毎に根を常
法によりトリパンブルーで染色し、ＶＡＭ菌との共生状
態を調べ、交点法により感染率を算出した。その結果を
第１表に示す。Example 1 Gigaspora margarita spores collected from a cucumber cultivation site were washed with sterilized water after foreign matter such as soil particles adhering to the surface was separated using an ultrasonic cleaner. Next, a vinyl pot with a capacity of 250m and a diameter of 9ai was filled with sterilized soil (particle size of 2n or less), and 2 edamame seeds were placed in it.
Seed 50, 200, and 50 Gigaspora margarita spores per pot as shown in Table 1.
After 0 inoculation, these pots were incubated at 25℃±2℃ for 1
Seedlings were grown for 7 weeks in a thermostat at 5,000 lux and 16 hours. Starting from the first week after inoculation, the roots were stained with trypan blue in a conventional manner every week to examine the state of symbiosis with the VAM bacteria, and the infection rate was calculated by the intersection point method. The results are shown in Table 1.
第１表
第１表より、接種したギガスポラ・マルガリタの胞子の
接種量が多いほど共生速度が速く、多く増殖しているこ
とが認められる。From Table 1, it can be seen that the greater the amount of inoculated Gigaspora margarita spores, the faster the symbiosis rate and the greater the number of multiplications.
実施例２
１／１０．０００ ａワグネルポットに滅菌処理した±
（１〜４日）１．５１肥料をＮＳＰ、０．、ＸｔＯとし
て各０．５ｇ、苦土石灰を２．０ｇ混合した培土を充填
した。充填した培土の中央にあらかじめ、２５〇−の小
型ポットにギガスポラ・マルガリタの胞子をポット当た
り５０個、２００個、５００個接接種て３週間育苗した
エダマメの苗を移植した。Example 2 1/10.000 a Wagner pot sterilized ±
(1-4 days) 1.51 fertilizer with NSP, 0. , 0.5 g each of XtO and 2.0 g of magnesia lime were mixed into the pot. In the center of the filled soil, edamame seedlings were previously inoculated into small 250-sized pots with 50, 200, and 500 Gigaspora margarita spores per pot and grown for 3 weeks.
移植後、２５℃±２℃、１５．０００ルツクス、日長時
間１０時間（短日処理）および１６時間（長日処理）の
恒温槽内で５週間栽培した。栽培終了後２週間放置し、
培土を乾燥させた後、培土からウェット・シーヴイング
法によりギガスポラ・マルガリタの胞子を分離した。そ
の結果を第２表に示す。After transplantation, the plants were cultivated for 5 weeks in a constant temperature bath at 25°C ± 2°C, 15,000 lux, and a daylength of 10 hours (short day treatment) and 16 hours (long day treatment). Leave it for two weeks after cultivation,
After drying the soil, Gigaspora margarita spores were separated from the soil by a wet sieving method. The results are shown in Table 2.
第２表
第２表より、短日処理区では胞子が形成され、長日処理
区ではまだ胞子が形成されていないことが認められる。Table 2 From Table 2, it can be seen that spores were formed in the short-day treatment area, but spores were not yet formed in the long-day treatment area.
また、接種量を増やし、ＶＡＭ菌とエダマメの根との接
触頻度を高めると、生成する胞子の数も増加することが
判る。Furthermore, it was found that as the amount of inoculation was increased and the frequency of contact between the VAM bacteria and the roots of edamame was increased, the number of spores produced also increased.
実施例３
１／１０．０００ａワグネルポツトに滅菌処理した土（
１〜４１ｍ）１．５１肥料をＮ、 ｐｚｏｓ、Ｋ、０と
して各ｏ、ｓｇ、苦土石灰を２．０ｇ混合した培土を充
填した。充填した培土の中央にあらかじめ、２５〇−の
小型ポットにギガスポラ・マルガリタの胞子をポット当
たり５０個接種して２５℃±２℃、１５．０００ルツク
ス、日長時間１６時間の恒温槽内で３週間育苗した青シ
ソの苗を移植した。Example 3 Sterilized soil (
1 to 41 m) 1.51 fertilizer was filled with soil containing 2.0 g of each o, sg, and magnesia lime mixed with N, pzos, K, and 0. In advance, 50 spores of Gigaspora margarita per pot were inoculated in the center of the filled soil into a small 250-cm pot, and the spores were incubated at 25°C ± 2°C, 15,000 lux, and a daylength of 16 hours in a constant temperature bath for 3 hours. Green perilla seedlings grown for a week were transplanted.
移植後２５℃±２℃、日長時間１０時間（短日処理）お
よび１６時間（長日処理）の恒温槽内で７週間栽培した
。栽培終了後、２週間放置し、培土を乾燥させた後、培
土からウェット・シーダイング法によりギガスポラ・マ
ルガリタの胞子を分離した。その結果を第３表に示す。After transplantation, the plants were cultivated for 7 weeks in a constant temperature bath at 25°C ± 2°C with daylengths of 10 hours (short day treatment) and 16 hours (long day treatment). After the cultivation was completed, the soil was allowed to stand for two weeks to dry, and Gigaspora margarita spores were separated from the soil by wet seeding. The results are shown in Table 3.
第３表
グロマス属の胞子をポット当たり５０個接種して２５℃
±２℃、１５．０００ルツクス、日長時間１６時間の恒
温槽内で３週間育苗したメヒシバの苗を移植した。Table 3: Inoculate 50 Glomus spores per pot at 25°C.
Seaweed seedlings were grown for 3 weeks in a constant temperature bath at ±2°C, 15,000 lux, and 16 hours of daylight, and then transplanted.
移植後、２５℃±２℃、日長時間１０時間（短日処理）
および１６時間（長日処理）の恒温槽内で７週間栽培し
た。栽培終了後、２週間放置し、培土を乾燥させた後、
培土からウェット・シーヴイングに法により、グロマス
属の胞子を分離した。その結果を第４表に示す。After transplantation, 25℃±2℃, day length 10 hours (short day treatment)
and cultivated for 7 weeks in a constant temperature bath for 16 hours (long day treatment). After cultivating, leave the soil for two weeks to dry,
Glomus spores were isolated from the soil by wet sieving. The results are shown in Table 4.
第４表
第３表より、短日処理区では、長日処理区と比べて、大
量の胞子を増殖していることが判る。From Table 4 and Table 3, it can be seen that a large amount of spores proliferate in the short-day treatment area compared to the long-day treatment area.
実施例４
１／１０，０００ａワグネルボフトに滅菌処理した土（
粒径１〜４ ｕ） １．５４！、肥料をＮ、 ｈａｓ、
ＫｇＯとして各０．５ｇ、苦土石灰を２．０ｇ混合し
た培土を充填した。充填した培土の中央に、直径９Ｃ１
ｌのビニルポットに滅菌処理した土２５０ｄを充填し、
第４表より、短日処理区では長日処理区と比べて大量に
胞子を増殖していることが判る。Example 4 Sterilized soil (1/10,000a Wagnerboft)
Particle size 1-4 u) 1.54! , fertilizer N, has,
The soil was filled with a mixture of 0.5 g each of KgO and 2.0 g of magnesia lime. In the center of the filled soil, place a diameter 9C1
Fill a liter vinyl pot with 250 d of sterilized soil,
From Table 4, it can be seen that spores proliferate in large quantities in the short-day treated plots compared to the long-day treated plots.
（発明の効果）
本発明方法により、ＶＡＭ菌を短期間で効率よく大量に
増殖させることができるばかりでなく、副次的に植物施
肥量を減少せしめ、土壌病害の発生防止にも大きく寄与
するものである。(Effects of the Invention) The method of the present invention not only makes it possible to efficiently multiply VAM bacteria in large quantities in a short period of time, but also reduces the amount of fertilizer applied to plants and greatly contributes to preventing the occurrence of soil diseases. It is something.
特許出願人 セントラル硝子株式会社Patent applicant: Central Glass Co., Ltd.
Claims (3)
う）を含む培土で、１日の日長が一定の長さ以下になっ
た時に花芽分化のみられる短日植物を栽培し、該植物の
栽培期間中の日長時間を短縮することで花芽分化を促進
させ、ＶＡＭ菌を培養することを特徴とするＶＡＭ菌の
増殖方法。(1) Short-day plants that exhibit flower bud differentiation when the daily day length falls below a certain length are cultivated in culture soil containing vesicle-arbuscular mycorrhizal fungi (hereinafter referred to as VAM fungi). A method for propagating VAM fungi, which comprises culturing VAM fungi by cultivating VAM fungi and promoting flower bud differentiation by shortening the day length during the cultivation period of the plant.
を栽培し、その根とＶＡＭ菌との接触頻度を高め共生さ
せた後、大型容器に移植する請求項１記載の増殖方法。(2) The propagation method according to claim 1, wherein short-day plants are cultivated in a small container filled with culture soil containing the VAM bacteria, and after increasing the frequency of contact between the roots and the VAM bacteria to coexist, the plants are transplanted into a large container.
個以上のＶＡＭ菌胞子を含有せしめる請求項１項および
２項記載の増殖方法。(3) At least 10 per 100 ml of soil in a small container.
3. The method of propagation according to claims 1 and 2, wherein the method comprises containing at least 5 VAM fungal spores.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4818489A JPH02227068A (en) | 1989-02-28 | 1989-02-28 | Proliferation of vesicular arbuscular mycorrhiza bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4818489A JPH02227068A (en) | 1989-02-28 | 1989-02-28 | Proliferation of vesicular arbuscular mycorrhiza bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH02227068A true JPH02227068A (en) | 1990-09-10 |
Family
ID=12796301
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4818489A Pending JPH02227068A (en) | 1989-02-28 | 1989-02-28 | Proliferation of vesicular arbuscular mycorrhiza bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02227068A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386245A (en) * | 2016-08-31 | 2017-02-15 | 韦永梁 | High-yield planting method for capsicum |
-
1989
- 1989-02-28 JP JP4818489A patent/JPH02227068A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386245A (en) * | 2016-08-31 | 2017-02-15 | 韦永梁 | High-yield planting method for capsicum |
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