JPH02219900A - Detergent composition containing enzyme and use of said composition - Google Patents

Abstract

PURPOSE: To obtain an oxygenous composition used for washing clothes, wherein the activity of the added lipase is relatively lowly inhibited, by mixing a surfactant (system) containing a prim. alcohol sulfate salt with a specified amount of a lipase and common detergent additives.

CONSTITUTION: This composition comprises 1-60 wt.% surfactant (system) containing desirably at least 30 wt.% prim. alcohol sulfate salt (e.g. coconut oil/alkyl prim. alcohol sulfate salt), lipase enzyme in an amount of 50-30,000 (LU) lipase unit per g of the surfactant system or the detergent composition and common detergent additives.

COPYRIGHT: (C)1990,JPO

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to detergent compositions and their uses, particularly lipase-containing compositions and their uses.

ni 1 good 1 Δ 1 fly Detergent compositions containing lipases are known.

For example, British Patent No. 1372034 (υn1lever
) has introduced Pseudomonas in detergent compositions containing certain nonionic surfactants to keep clothes soaked.
discloses formulating lipase from.

U.S. Patent No. 3,950,277 (Procter a G
Amble) describes garment soaking compositions containing lipases and lipase activators, and lists a number of lipases from microbial and other sources. Particularly suitable examples are ^1Ilano C
E, ^nano M-^P, Takeda 1969
-4-9 and Meito MY-30 lipase, but the production or usage form of the lipase is not pointed out.

US Patent No. 4011169/Netherlands 740876
No. 3 (ProcLer & Gamble) describes the use of a similar range of enzymes in the production of additives for cleaning agents (detergent compositions).

An example of a known lipase-containing detergent composition is PsNuore
scens, P gliidioli and Chro+
European Patent Nos. 0205208 and 0206390 (Llni 1ever) relate to the incorporation of lipases derived from P. nobacter into detergent compositions.

European Patent No. 0214761 (NOVO) and European Patent No. 0258068 (Novo) each describe in detail lipases from certain microorganisms and the use of the described enzymes in detergent additives and detergent compositions. is also disclosed. European Patent No. 0214761 is based on I'seudomonas ce
Lipases derived from microorganisms of the Pacia species and their uses are described in detail. European special [
No. 0258068 is Thermomyces/1lu
lipase derived from microorganisms of iaicolafi;
and its use is described in detail.

Additionally, in some fields, about 37% detergent actives, including 5% non-ionic detergents and the remainder substantially anionic detergents, about 16% zeolites, about 60 LIJ/liter of lipases, proteases and others. The use of lipase-containing granular detergent compositions containing the usual detergent additives is warranted.

Another example of a known lipase-containing detergent composition is Pseud
JP 63-078000 (1988) (Lion Corp.) disclosing the properties and uses of omonas lipase.
/K Mukoyama et al.), whose use is based on 10-40% surfactant (e.g. C12-
18, e.g. sodium C14-C18 alpha-olefin sulfonate) and other conventional detergent ingredients.

Furthermore, as a prior art, European Patent No. 026845
No. 8 (C1orox), and this document is No. 10(b)
The experimental wash solution containing lipase and approximately 1 μg 7 ml sodium dodecyl sulfate is shown in the table.

Furthermore, as prior art, European Patent No. 0179533
Re5ea, June 1988, disclosing lipases in connection with detergents (Procter & Gamble).
rcl+ Disclosure29056.

One problem with conventional lipase-containing detergent compositions is that
The point is that many detergent active substances have a strong tendency to inhibit the action of lipases.

An object of the present invention is to provide a detergent composition in which the activity of added lipase is relatively less inhibited.

According to the present invention, there is provided an enzyme-containing detergent composition for cleaning clothes, which composition comprises, in particular, primary alcohols, alone or in mixtures, having a chain length of C128, e.g. C14 to C18. 1 to 60% by weight of a surfactant or surfactant system containing a sulfate ester salt and 50 to 300% by weight per g of surfactant system or detergent composition.
00 (LIJ) lipase units.

As used herein, the lipase unit has the same meaning as in Yoguchi-Soba Patent No. 0258068 (Novo).

In most embodiments, the ingredients of the detergent composition further contain one or more conventional detergent additives, such as those listed below.

In some preferred embodiments, particularly in certain detergent powder or granular formulations, the surfactant system in the detergent composition consists essentially of a large proportion, especially 50% or more, of the primary alcohol sulfate esters mentioned above. It may consist of salt. In certain other useful embodiments, the proportion of primary alcohol sulfate salt in the surfactant system may be lower, such as about 2
5-30% or more, for example about 33% or more, for example about 40%
That's all.

It has been found that lipase enzymes exhibit surprisingly low inhibition in these systems. One reason for the surprising good performance of lipase in this system is that at least one member of the class of primary alcohol sulfate salts, namely sodium dodecyl sulfate, is well known and used in laboratory studies as a denaturing agent for proteins, including enzymes. It is used in α−
Compared to lipase-containing surfactants based on olefin sulfonates, the compositions of the invention have a strong cleaning action and a sufficiently low level of lipase inhibition.

Even more surprisingly, primary alcohol sulfate salts have a (known) builder effect, despite their high calcium affinity compared to the highly inhibiting LAS surfactants. The degree of lipase inhibition does not increase significantly.

The primary alcohol sulfate salts used in these compositions are derived, for example, from coconut oil-primary alcohol sulfate salts or tallow-primary alcohol sulfate salts or mixtures of these with each other and/or from pond sources. may be based on analogs with alkyl chains.

Minor or minor amounts, e.g. up to 10% of the surfactant system,
Optionally up to 20% or 35%, even up to 50%, of other surfactant types, especially anionic, nonionic or cationic detergents, can also be incorporated into the composition, e.g. Linear alkylbenzene sulfonates (LAS) or (branched f/A) alkylbenzene sulfonates (80S), which have been found to exhibit unacceptably high degrees of lipase inhibition, can be included.

Some examples of compositions of the invention, in particular the following implementation ρ11
In the examples shown in 1 to 13, the amount of alkylbenzene sulfonate (LAS or ^BS) is about 66% of the surfactant system containing the primary alcohol sulfate salt (PAS).
%, in some cases up to about 70% or even 75%. For example, some formulations may contain a surfactant system consisting essentially of a mixture of PAS and BS and/or LAS. In this case, the proportion of ^BS or LAS in such a mixture may be, for example, up to about 75%, for example up to about 70%, and often slightly lower, for example up to about 66 or 60% by weight.

A preferred surfactant system for use in the present invention is C12-C
Based on a mixture of 14 coconut oil-alkyl and C1B-C18 taro-alkyl sulfates (e.g. about 1+1 iv/w).

The lipolytic enzyme is usefully added as a granular composition of the lipolytic enzyme along with a carrier material.

The amount of lipolytic enzyme added can be selected within a wide range, for example from 50 to 30,000 L per g of granular detergent composition.
U, e.g. often at least 100 LU/g, very usefully at least 500 LII/)7, sometimes preferably 1000 or more, 2000 LU/g or more, or 40001.179 or more, i.e. very often is 50-4000LLl/g, in some cases 2
00~1000L [I/l? It is.

The lipolytic enzyme can be selected from a wide range of lipases, in particular those described in European Patent No. 0214761 (
Novo), European Patent No. 0258068 (No.
vo), in particular as described in European Patent No. 0205208 (Unilever) and European Patent No. 0206390 (Uni 1ever).
Thermonyces l as described in )
A lipase that shows immunological cross-reactivity with the antiserum against lipase from Chromoba Cter viscos, C. anuginosus ATC, C22070.
unwar Iipolyticum NRRL B-
Lipase or ^lcaligene from 3673
s PL-679, ^TCC31371 and FERN-
Lipases showing immunological cross-reactivity with antisera against lipases from P 3783 and -0871008
59 (Gist-Brocades) and European Patent No. 0204284 (Sapporo Brewer
For example, the commercially available lipase preparation Novo Lip
olase, ^mano lipase CE, P, B, ^P
, To^P,^ML and CES, Meito Lipase M
Y-30, OF and PL, also esterase MM, L
ipozyn, 5P225.5P285.5aikeu
Lipase, Enzec.

Lipase, Toyo Jozosu ° Barse and Dio
sytiLh lipase (both registered trademarks) is particularly suitable.

Enzyme gene set +a is a suitable lipase gene, e.g.
Extraction of lipase gene from hermomyces lanugInosus or its mutant strain 5 and Δ
This can be carried out by introducing and expressing the gene or a derivative thereof in a suitable production organism such as 5ppr8il1us. H088102775 (Novo), European Patent No. 0243338 (Labor 1na) and European Patent No. 0268452 (Cenenco)
The method described under r) can be applied.

One preferred non-limiting example of a granular or powdered detergent composition of the present invention is 9% primary Al A. Sourium sulfate, 6.7% sodium carbonate, 3.4% sodium chloride, 6.5% C-sodium silicate, 30.2% zeolite^ (anhydrous), 4.5% 5okolan C
F2 (registered trademark), 4% Synperonic A4 (
registered trademark, Cl2-E4 polyethoxylated alcohol), 1% lipase (Lipo!ass from Novo)
30TWi grains) or Pseudoeionasglad
Contains lipase from Ioli.

The detergent composition may further contain conventional detergent ingredients in conventional amounts, such as those listed below. The detergent composition may be built or unbuilt, and may be phosphorus-free (ie, free of phosphorus-containing building blocks). Thus, the composition may contain from 1 to 45%, for example from 5 to 30% by weight, of one or more organic and/or inorganic building blocks. Typical examples of such virgoos include alkali metal ortho, pyro and tripolyphosphates, alkali metal carbonates alone or in mixtures with calcite, alkali metal citrates, alkali metal nitrilotriacetate-1, carboxymethyloxy Examples include succinate, zeolite, and polyacetal carboxylate. Additionally, the composition may contain from 1 to 35% of a bleach, a bleach precursor, or a system containing a bleach and/or precursor and its activator. Other optional ingredients include foam enhancers, foam inhibitors, corrosion inhibitors, soil suspending agents, sequestering agents, anti-resoiling agents, fragrances, dyes, enzyme stabilizers, and the like. Examples of enzymes that may be present other than lipase are protease, amylase,
oxidase and cellulase.

The composition can be made in any desired way, eg powder, bar, base! - Can be formulated as a liquid.

The composition can be used for cleaning textile materials, but in particular, but not limited to, cotton- and polyester-based m-fibers and mixtures thereof. The cleaning process is for example about 60-65℃
It is particularly suitable to carry out the following, for example at temperatures below about 30-35°C.

Hereinafter, the present invention will be further explained in a non-limiting manner by way of examples.

It should be noted that these examples show that the test fabrics were contaminated with lipids and then washed five times with a number of different lipase-containing detergent formulations.
The performance expressed as reflectance of 111- is also shown.

In each example, a cotton test fabric was contaminated with an olive oil-based fat mixture (up to 1% by weight of the test fabric) and washed using a detergent formulation after three pre-washes. did. The detergent formulations used contained the following different surfactant systems (9% by weight each): in Example 1, coconut oil-alkyl sulfate (^
In Example 2, tallow-alkyl sulfate (5ulpl+opon from Henke 1)
T55), 50:50 coconut oil-alkyl/tallow-alkyl sulfate in run M3, 25:25:5 in example 4
0 coconut oil-alkyl-tallow alkyl-DOB113, corresponding to the formulation detailed above, except that Example 5 used DOB113 (linear alkylbenzene sulfonate alone for comparison).

In the above formulation, DOB113 refers to DOB113®, a sulfonated product of alkylbenzene from Shel 1 Company.

Examples 6 to 10 are the same as Examples 1 to 1 except that the washing temperature was changed.
Corresponds to 5.

In each example, 3 g/l of pre-dissolved detergent composition was used with or without lipase enzyme.
Isothermal cleaning was performed at 0°C or 60°C for 30 minutes. cloth/
Liquid ratio was consistently 10:1 with 50:50 contamination;
The amount of charge was assumed to be non-contaminated.

In each experiment, Lipolase from NoVO Co., Ltd., or the lipase from Pseudot*onasgladio, which was conventionally purified with a lipase activity equivalent to Lipolase, was used as the culture medium preparation P, ff, (800 Lυ/zy).

460 from 5 specimens after 5 soil/clean cycles
The results were expressed as the average reflectance obtained with old n.

Examples 1 to 5 were carried out at 60°C, and Examples 6 to 10 were carried out at 30°C.

Sitting on your back] Kei■ν Nekoe Control (no lipase) Experiment (Lipolase) (gladioli) Example 1 Example 2 Example 3 Example 4 Example 5 68.3 69.6 68.7 71.8 73. 8 72.8 74.0 71.9 73.4 73.9 73.5 74.2 72.8 Example 6 Example 7 Example 8 Example 9 Example 10 G9.2 70.6 69.6 70 .5 72.3 72.1 70.9 71.9 71.2 73.7 73.6 73.4 73.2 Results indicate, in part, increased benefit due to lipolytic activity.

- most likely, lipase in the presence of detergents of the LAS type has substantially less benefit than the average of the other examples (primary alcohol sulfate salts and mixtures thereof) (Example 5.10); These other embodiments are therefore more suitable for use.

Detergent bar formulations with a good 11% or lower ratio complement well the lipolytic action of lipase.

Primary alcohol sulfate salt (PAS) 28
15.5 12 For example, KAO's EMΔLIO1
Average chain length C10) alkylbenzene sulfonate (^B
S) -15,518 (e.g. Exxon's E
scan F, average chain length C11) Sodium birophosphate 16 10 10 Soda ash io ia
is calcite 3
2 31 31 aluminosilicate
2 2 2Lipolase
1 1 1 1 for each example
The secondary ingredients and wood formulations up to 0.00% were formulated in a manner known per se for detergent bars.

The invention is capable of variations and extends to the use of all combinations and sub-combinations of the features described herein and, where appropriate, each of the above-mentioned features incorporated herein by reference. Cited published patent Mei 11 (B
May be combined with each of the features of the calligraphy.

Claims (14)

[Claims] (1) An enzyme-containing detergent composition for cleaning clothes, comprising 1 to 60% by weight of a surfactant or surfactant system containing a primary alcohol sulfate salt, and a surfactant system or detergent composition. An enzyme-containing composition containing lipase enzyme in an amount of 50 to 30 000 (LU) lipase units per g and conventional detergent additives. (2) The primary alcohol sulfate salt forms at least 30% of the surfactant system.
The composition described in . (3) The primary alcohol sulfate salt forms at least 50% of the surfactant system.
The composition described in . (4) The primary alcohol sulfate salt is C12 to C1
4. A composition according to any one of claims 1 to 3, characterized in that it has a chain length in the range of 8. (5) The primary alcohol sulfate salt contains alkyl chains derived from coconut oil-alkyl primary alcohol sulfate salt, tallow-alkyl primary alcohol sulfate salt, mixtures of these with each other, and/or other sources. 4. A composition according to any one of claims 1 to 3, characterized in that it contains an analogue of the composition. (6) Furthermore, a surfactant-based secondary component of the detergent composition (50%
6. Composition according to claim 1, characterized in that it contains an anionic, nonionic or cationic detergent as (up to %) anionic, nonionic or cationic detergent. (7) The composition according to claim 6, which is in the form of a granular composition or a powder. (8) the surfactant system comprises or consists essentially of a mixture of a primary alcohol sulfate salt and a linear or branched alkylbenzene sulfonate, the proportion of the primary alcohol sulfate salt being a surfactant; Composition according to claim 1, characterized in that it is at least 25%, such as at least 30%, of the weight of the system. (9) The composition according to claim 8, which is in the form of a bar or paste. (10) Lipase is Thermmyces lanug
showed immunological cross-reactivity with antiserum against lipase from S. inosus ATCC22070 or Ch.
romobacter viscosum var li
10. The composition according to any one of claims 1 to 9, characterized in that it is a lipase that exhibits immunological cross-reactivity with an antiserum against a lipase from P. polyticum NRRL B-3673. 11. Composition according to claim 10, characterized in that the lipolytic enzyme is added together with the carrier material in the form of a granular composition of the lipolytic enzyme. (12) Primary alkyl sulfate, zeolite A (anhydrous),
Sodium sulfate, sodium carbonate, sodium chloride, C
- Sodium silicate, Sokolan CP5®, Synperonic A4® and L
12. Composition according to any one of claims 1 to 11, characterized in that it is in the form of a detergent powder or granules containing ipolase (Novo). (13) Primary alcohol sulfate salts (PAS) (e.g. Kao's EMAL 10, average chain length C10), alkylbenzene sulfonates (ABS) (e.g. Exx
on's Escane F, average chain length C11), sodium pyrophosphate, soda ash, calcite, aluminosilicate,
12. Composition according to any one of claims 1 to 11, characterized in that it is in the form of a detergent bar containing Lipolase, trace ingredients and water. (14) Any one of claims 1 to 8, characterized in that it contains 1 to 35% of a bleach, a bleach precursor, or a system containing a bleach and/or a precursor and an activator thereof. Compositions as described.