JPH01261499A - Detergent composition - Google Patents

Abstract

PURPOSE:To improve detergency against an oil stain and to suppress a lowering in the lipase activity during storage for a long period of time, by blending an anionic surfactant with a lipase in a predetermined ratio. CONSTITUTION:10-30wt.% salt of a straight-chain (10-14C) alkylbenzenesulfonic acid (a) is blended with 5-15wt.% salt of 10-18C alkylsulfuric acid (b) wherein 50wt.% or more of the alkyl group has 12-14C, in an (a) to (b)wt. ratio of 5-1/1 to prepare an anionic surfactant. This surfactant is blended with an alkali lipase in an amount of 1000-1000000 units per kg of the detergent and having such a lipase activity that the lipase activity at a pH value of 9 is 30% or more of that at a pH value of 7.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a cleaning composition containing lipase as an enzyme.

In recent years, due to the problem of eutrophication of environmental waters, the amount of condensed phosphates contained in heavy household detergents has been significantly reduced or eliminated, and now phosphorus-free detergents that use zeolite as a builder are mainstream. It has reached the point where it now occupies the majority. This zeolite removes calcium ions, which are the hardness component in the washing liquid, by ion exchange, and prevents the anionic surfactant from turning into a poorly soluble calcium salt and reducing the washing power.

In addition, so-called polymer builders such as polyacrylates and copolymers of maleic anhydride and olefin are used together with zeolites as phosphorus-free calcium ion-trapping builders other than zeolites.

However, it is desired to further improve the detergency, and studies have been conducted from the viewpoint of surfactants, builders, and additives such as enzymes.

Sulfonic acid salts of lower alkyl esters of saturated fatty acids are α
-Also called sulfo fatty acid ester salt, it is a surfactant with excellent detergency, especially hard water resistance, and many detergent compositions have been proposed using it as a main active ingredient. Also, α−
A high bulk density detergent composition containing a sulfo fatty acid ester salt has also been reported (Japanese Unexamined Patent Publication No. 62-599).

Research is also being conducted focusing on various dirt components based on cleaning theory, and among the dirt components, proteins, lipids,
In particular, triglycerides are said to be difficult to wash. Among these, the use of protein-degrading enzymes (proteases) has been considered for some time, and there are many examples of their use in detergents. Furthermore, it is possible to decompose lipids with a lipolytic enzyme (lipase) and remove them as fatty acids.

However, in terms of cleaning power, especially oil stain cleaning power, there was still further improvement.

It is also important to prevent the lipase activity from decreasing over time and to stably incorporate the lipase into the detergent composition.

DISCLOSURE OF THE INVENTION The present invention provides a cleaning composition with excellent oil stain cleaning ability.

The present invention provides an enzyme-containing detergent that suppresses a decrease in lipase activity due to long-term storage.

Structure of the Invention The present invention significantly improves oil stain cleaning power by using a specific surfactant system and lipase in combination. Specifically, the following components (A) and (B) are used together. This is a cleaning composition (LAS/AS/alkaline lipase) used in combination.

(A) At least the following (A”) and (A2) as anionic surfactants [However, the weight ratio is (A1)/(
A”) = 5/1 to 1/1).

(A″) Linear alkylbenzene sulfonate: 10~
30% by weight.

(A2) Alkyl sulfate in which the number of carbon atoms in the alkyl group is C1°-1, of which C12-14 accounts for 50% by weight or more: 5 to 15% by weight.

(B) an alkaline lipase whose lipase activity at pH 9 is 30% or more of the lipase activity at p)+7, and the following (a) and (b) in place of component (A);
A detergent composition with excellent oil stain cleaning power can also be achieved by combining this with component (c) [same as component (B) above] (α-8F/nonionic/alkaline rehabilitation). Se).

(a) Sulfonic acid salt of lower alkyl ester of saturated fatty acid having 8 to 22 carbon atoms as fatty acid residue: 5 to 30% by weight.

(b) Alcohol ethoxylate in which an average of 10 to 30 moles of ethylene oxide is added to a primary or secondary alcohol having 8 to 18 carbon atoms: 1 to 15% by weight The invention further provides (α) an anionic surfactant and (γ)
It also includes cleaning compositions (anion/nonion, PEG/alkaline lipase) in which the following component (β) is added to a cleaning composition containing alkaline lipase particles to prevent a decrease in lipase activity during long-term storage. .

(β) Alcohol ethoxylate with an average of 7 to 30 moles of ethylene oxide added to a primary or secondary alcohol having 8 to 18 carbon atoms; and/or a molecular weight of 2000 to
20000 polyethylene glycol: 2-10% by weight
.

The present invention will be explained in detail below.

i LAS/AS/alkaline lipase'' group (A1
) Linear alkylbenzene sulfonate (LAS) (A2) Alkyl sulfate (AS) (B) Alkaline lipase (A1) Linear alkylbenzene sulfonate includes:
An alkyl group having 10 to 14 carbon atoms is suitable, and the salt is preferably sodium or potassium. . (A
1) The linear alkylbenzene sulfonate is present in the detergent composition in an amount of 10 to 30% by weight, or 15 to 25% by weight.
It is blended. If this amount is less than 10% by weight, the detergency will be low, while if it exceeds 30% by weight, production will be repeated.

(A2) As the alkyl sulfate, the number of carbon atoms in the alkyl group is C□. ~□. And among them, C□2~1. occupies 50% or more, preferably 60% or more. salt(
As the counter ion, sodium and potassium are preferable. Alkaline lipase loses its activity in linear alkylbenzene sulfonic acid and becomes less effective in improving its detergency, but by using the alkyl sulfate with the above-mentioned specific carbon chain length in a specific amount ratio, the detergency of oil stains can be improved. improves.

(A2) The amount of alkyl sulfate in the cleaning composition is 5 to 15%.
% by weight is added.

Furthermore, (A1) linear alkylbenzene sulfonate and (A2) alkyl sulfate have a weight ratio of (A1)/(A
”) = 5/1 to 1/1. It is preferably blended in the range of 4/1 to 2/1. If this ratio is larger than 571, the effect of improving oil stain detergency cannot be obtained; /J% from /1
It has low foaming and is undesirable.

(B) As for the alkaline lipase component, the lipase activity at pH=9 is 30% higher than the lipase activity at pl=7.
% or more, preferably 50% or more.

Lipase activity at pH 7 and pH 9 can be measured as follows.

(mon) Lipase activity measurement method Using olive oil as a substrate, the fatty acids liberated by lipase action are quantified by alkaline titration, and the lipase activity is determined from this value.

One enzyme unit is an enzyme that releases 1 MM of fatty acid per minute from the substrate olive oil at 37°C.

Olive oil emulsion 4IIIQ and 0.1M phosphate buffer (p
Accurately place 4 m 12 of H7,0) in a 50 m A-capacity stoppered Erlenmeyer flask, mix well, and preheat for 10 minutes using a 37°C constant temperature water bath. Add sample solution IIIQ accurately to this, mix well, and titrate with acetone/ethanol mixture after exactly 20 minutes.

Separately, 5 fflρ of olive oil emulsion and 4 rrr Q of 0.1 M phosphate buffer (pH 7,0) were accurately placed in a 50 m Q volume stoppered Erlenmeyer flask, and after heating at 37°C for 30 minutes, 20 m Q of acetone/ethanol mixture was poured into the flask.

Next, add sample solution IIIQ accurately, use 5 drops of phenolphthalene test solution as an indicator, and titrate with 0.05N sodium hydroxide test solution to use as a control solution.

Calculation of enzyme unit Lipase activity (unit/g) = (sample solution titration value - control solution titration value) When measuring at pH = 9, use 0.2M Tris buffer to set pH = 9, The rest was carried out in accordance with the above method.

As the lipase suitable for the present invention, Candidacy
lindracea, Humicola lanug
inosa.

Psdomonas, Mucor sp,, Ch.
romobacterium viscosum, Rh
There are those of microbial origin such as 1zopus japonics, lipase OF, lipase OF (Candida cylindracea, Meito Sangyo Kyusei),
Lipase P, Lipase CES (manufactured by Amano Pharmaceutical,
origin Pseudomonas), lipase SP (manufactured by Novo, origin Mucor sp, ) - lipase (manufactured by Toyo Jozo ■, origin Chromobacterium visc)
osum), Lipase LPL (manufactured by Oriental Yeast ■), Olivase (Osaka Bacteriological Research Institute, origin Rh1zop)
japonics), Lipase B (manufactured by Sarapolo Beer, origin: Pseudomonas), etc. In addition, Japanese Patent Publication No. 53-45394 describes the method of culturing lipase-producing filamentous fungi belonging to the genus Humicola (including the genus Thermomyces) and collecting lipase from the culture; Orchid Inosus strain 5-38 (Feikoken Bacterial Serial No. 1045
(No.) has been reported, but such lipases can also be used.

Furthermore, lipases produced from genetically modified microorganisms can also be used. As an example of this, JP-A-62-27
In No. 2988, Aspergillus oryza (Aspergillus oryzae)
gillus oryzae) as a host, and the recombinant DNA
It has been reported that lipase can be produced by transforming cells using the A technique and culturing them in a culture medium.

The amount of alkaline lipase (B) component is 1 kg of detergent.
It is preferable to mix in an amount of 10,000 to 1,000,000 lipase units (LU) per lipase unit.

Various optional components can be added to the above-mentioned cleaning composition as necessary. Optional ingredients include surfactants other than component (A) such as anionic surfactants, nonionic surfactants, and cationic surfactants, zeolite, and water-soluble phosphorus-free calcium-trapping chelate builders; other than the alkaline lipase of the present invention. enzymes; bleaching agents such as sodium percarbonate and sodium perborate, reducing agents such as sulfites, recontamination prevention agents such as carboxymethyl semerose and polyethylene glycol, soaps, optical brighteners, bentonite, fragrances, etc. Can be used as needed.

Examples of anionic surfactants other than component (A) include olefin sulfonate salts, alkyl (or alkenyl) sulfate ester salts, and α-sulfo fatty acid ester salts.

Examples of nonionic surfactants include the following.

(1) An EO-added nonionic surfactant in which an average of 7 to 30 moles of ethylene oxide (EO) is added to a primary or secondary alcohol having 8 to 18 carbon atoms.

(2) EO-P○ addition type nonionic interface in which an average of 7 to 20 moles of ethylene oxide (EO) and an average of 3 to 15 moles of propylene oxide (po) are added to a primary or secondary alcohol having 8 to 18 carbon atoms. Activator.

As the zeolite, crystalline or amorphous aluminosilicate represented by the following general formula (n), or a mixture thereof is suitable.

x (M, x○ or M'○) - AQ, ○, -y (sio
, )・w(H,o)-(n) (In the formula, M is an alkali metal atom, M' is an alkaline earth metal atom exchangeable with calcium, and X+3' and 2 are the number of moles of each component. represents, generally or 0.7 to 1.5. y is 1 to 3
, W is an arbitrary number 6) The average particle size of zeolite is 5μ or less in terms of detergency,
The thickness is preferably 1 μm or less.

Examples of water-soluble phosphorus-free calcium ion scavenging builders include aminopolyacetates such as nitrilotriacetate, ethylenediaminetetraacetate, and diethylenetriaminepentaacetate; polyvalent carboxylates such as citrate; polyacrylates; Hydroxy polyacrylates, polyitaconates, polyacetal carboxylates, salts of copolymers of acrylic acid and maleic anhydride, salts of copolymers of maleic anhydride and methyl vinyl ether, copolymers of maleic anhydride and olefins Polyelectrolytes such as polymer salts and copolymer salts of acrylic acid and methacrylic acid are included.

The above chelate builders may be used alone or in combination of two or more.

In addition to the alkaline lipase of the present invention, other enzymes such as protease, cellulase, amylase, etc. can be added as enzymes.

The cleaning compositions of the present invention can take a variety of forms, such as granular detergents in the form of hollow beads, such as conventional spray-dried detergents. Also, JP-A-60-9
As shown in Japanese Patent No. 6698, it is also possible to produce a high bulk density granular detergent in which the detergent components are packed even inside the detergent particles.

In the case of a high bulk density cleaning composition, the bulk density is 0.5 to
1.2 g/cc is suitable, and such compositions are disclosed, for example, in Japanese Patent Application Laid-open No. 60-96698 and No. 60-96698.
As described in Publication No. 2-597, each detergent component is kneaded and mixed in a kneader, then crushed and granulated in a cutter mill type crusher, and then coated with water-insoluble fine powder. It can be manufactured by The detergent ingredients can also be supplied in the form of a spray-dried product and kneaded.

Components (A1), (A'') and (B) of the present invention can coexist in various forms, but (A1) and (A2
It is preferable that component ) and component (B) are separately granulated together with other additive components to form detergent particles and lipase particles, respectively, and these are mixed into granules to form a cleaning composition. ) Alkaline lipase can be granulated using conventional techniques.

...α-SF/nonionic/alkaline lipase°° 1 pair.

(a) Sulfonate of a-Japanese fatty acid lower alkyl ester (α-5F) (b) Alcohol ethoxylate (nonionic) (Q
) The sulfonate of saturated fatty acid lower alkyl ester of alkaline lipase (a) component is typically represented by the following formula (I).

R1-CHOOR” SO□M (1) (R1: C, ~2° alkyl group R”: C, between lower alkyl groups: counter ion) Among these, fatty acid residues with carbon numbers of 12 to 22 (R1:
C1,2,) are preferred, and also.

Preferred counterions are alkali metals, especially sodium salts.

(a) The sulfonate of a lower alkyl ester of a fatty acid is prepared by converting a fatty acid having 8 to 22 carbon atoms into a lower alkyl ester, and then reacting it with sulfuric anhydride using an ordinary sulfonation device, and aging it if necessary. Obtained by bleaching and then neutralizing. It can also be obtained by converting a sulfonated saturated fatty acid into a lower alkyl ester.

(a) Sulfonate of saturated fatty acid lower alkyl ester (α-5F) is blended in the cleaning composition in an amount of 5 to 30% by weight, preferably 10 to 20% by weight.

If this amount is less than 5% by weight, the performance of α-8F cannot be fully exhibited, while if it exceeds 30% by weight, the rinsability will deteriorate.

Moreover, in addition to α-3F, other anionic surfactants can also be used in combination, and specific examples thereof include the following.

1) Straight-chain alkylbenzene sulfonate having an alkyl group having an average carbon number of 8 to 16.

2) an olefin sulfonate having an average carbon number of 10 to 20; 3) an alkyl sulfate having an average carbon number of 10 to 20; 4) having a linear or branched alkyl group or alkenyl group having an average carbon number of 10 to 20. , an alkyl ether sulfate or an alkenyl ether sulfate to which an average of 0.5 to 8 moles of ethylene oxide has been added. As counter ions in these anionic surfactants, sodium, potassium, ethanolamine, ammonium, etc. can be used.

(b') Alcohol ethoxylate (nonionic surfactant) has 8 to 18 carbon atoms, preferably 10 to 16 carbon atoms.
Ethylene oxide (E
O) is added on average from 10 to 30 moles, preferably from 10 to 20 moles.

(b) Alcohol ethoxylate is blended in the cleaning composition in an amount of 1 to 15% by weight, preferably 3 to 7% by weight.

If this amount is less than 1% by weight, the detergency against oil stains cannot be sufficiently improved, while if it exceeds 15% by weight, the detergent tends to harden.

(c) Alkaline lipase is the same as that already explained as component (B).

The optional components, the form of the detergent, and the coexistence of each component are the same as those already described. (a), (b) circumferential components, and (c)
It is preferable to separately granulate the components together with other additive components to form detergent particles and lipase particles, respectively.

Ω round − Otsu 2 Otsu 4 N = G minute j Su 4 Shibu J Nakabe mutual panisato (
α) Anionic surfactant (β) Alcohol ethoxylate (nonionic) and/or polyethylene glycol (PEG) (γ) Alkaline lipase particles When lipase particles are added to detergent particles containing an anionic surfactant, lipase particles can be reduced by long-term storage. Although a decrease in lipase activity is observed, it has been found that the decrease in lipase activity can be suppressed by incorporating a specific nonionic surfactant or polyethylene glycol.

(α) Anionic surfactants are those used as cleaning active ingredients, and include linear alkylbenzene sulfonates, olefin sulfonates, alkyl sulfates,
Alkyl (or alkenyl) sulfates, α-sulfo fatty acid ester salts, and the like are used. (α) The anionic surfactant is blended in the cleaning composition in an amount of 10 to 50% by weight, preferably 20 to 50% by weight.

Among the (β) components, the nonionic surfactant is a primary or secondary alcohol having 8 to 18 carbon atoms (preferably 10 to 16 carbon atoms), and an average of 7 to 3 ethylene oxide (EO).
An EO-added nonionic surfactant (alcohol ethoxylate) with 0 mol (preferably 10 to 20) added is used. Also, polyethylene glycol having a molecular weight of 2,000 to 20,000 is used.

These (β) components are blended alone or in combination in the detergent composition in an amount of 2 to 10% by weight, preferably 3 to 7% by weight. If this amount is less than 2% by weight, a decrease in lipase activity during long-term storage cannot be prevented. On the other hand, if it exceeds 10% by weight, it will be disadvantageous in terms of cost.

(γ) Alkaline lipase is granulated and added to detergents due to problems with stability and decreased activity during production. The granulation method of enzyme particles has been described in many documents, and
It is described in detail in 0-262900 and 61-34097.

For example, an inorganic salt such as Ca5O and NaCQ is added to enzyme powder, a binder such as water is added, mixed and granulated,
Furthermore, by coating the surface with polyethylene glycol, titanium oxide, etc., the average particle size is 500 to 100.
Lipase enzyme particles of 0 μm can be obtained.

As alkaline lipase in the present invention.

The same components as those already explained as component (B) can be used.

(γ) As alkaline lipase particles, the activity is 1
,000~100,000 units/g, 0.05
-5% by weight, preferably 0.1-1% by weight in the detergent. If this amount is less than 0.05% by weight, the effect of improving detergency is poor, and if it exceeds 5% by weight, it is disadvantageous in terms of cost.

According to the present invention, (A1) linear alkylbenzene sulfonate and (A2) alkyl sulfate and (B
) By using it in combination with alkaline lipase, the detergency against oil stains can be significantly improved.

In addition, in place of the above components (A') and (A2), (a) a salt of a sulfonated fatty acid lower alkyl ester and (b)
By using a specific nonionic surfactant in combination, the ability to clean oil stains can be significantly improved as well.

Furthermore, by incorporating (γ) alkaline lipase particles into detergent particles containing (α) anionic surfactant and β) specific nonionic surfactant or polyethylene glycol, it is effective to reduce lipase activity during long-term storage. Hereinafter, the present invention will be described in more detail with reference to Examples. Prior to this, the evaluation method used in the Examples will be explained.

(Left below) <Oil-based stain cleaning power> (i) Preparation method for oil-based stains 30 g of soybean oil (pharmacopoeia) and 0.25 g of carbon black are ultrasonically dispersed in IQ benzene for 5 minutes to form a stable dispersion. Create a filth bath.

A predetermined clean cloth (Loan x 20 cm) (cotton cloth No. 60 designated by Japan Oil Chemists Association) is inseminated in this dirt bath and then air-dried. After drying this soiled cloth at 105° C. for 30 minutes, both sides of the soiled cloth were rubbed 25 times on each side. This was cut into 5cm x 5CI11 pieces with a reflectance in the range of 42±2%, which was used as a contaminated cloth.

(ii) Cleaning method U, S, Terg-0-Tomet from Testing
Using an ER, add 10 pieces of oil-stained cloth and knitted cloth, adjust the bath ratio to 30 times, and heat at 120 rpm and 25°C.
Wash for 0 minutes.

The cleaning solution is 900m with a cleaning agent concentration of 0.083% 12
Rinse with 900 ml of water for 3 minutes.

The water used is 3' DH.

(iii) Calculation method of detergency R is the reflectance (%) measured by a Cari Zeiss ELREpHO reflectance meter.

Note that the detergency was evaluated using the average value of 10 sample oil-stained cloths.

<Evaluation method of lipase residual activity> Lipase-containing detergents produced under various conditions were placed in cartons (
22■X 15.50×50, moisture permeability 30g/Bo・24
hrs) and stored in a humidity box at 35°C and 85% RH for one month. After storage, the lipase activity is measured by the method described above, and the survival rate is calculated from the value before storage. In addition, in the measurement, in order to exclude the influence of detergent components, only enzyme particles were selected and used as a sample.

Example 1 (LAS/AS/Lipase) (1) Preparation of detergent particles A detergent slurry with a solid content of 45% was prepared using each component obtained by removing the nonionic surfactant from the high bulk detergent particles shown in Table 1 below. Prepared. This detergent slurry was dried using a countercurrent spray drying tower at a hot air temperature of 380° C. to a moisture content of 5 ml to obtain a spray-dried product.

This spray-dried product has an average particle size of 350 μm and a bulk density of 0.3.
The fluidity was also good, with an angle of repose of 5 g/cc and 45 degrees.

Next, the above dried product, nonionic surfactant and water were added,
The mixture was introduced into a continuous kneader (manufactured by Kurimoto Iron Works, KRC kneader #2 type) to obtain a dense and uniform kneaded product.

A perforated plate (thickness: 10 rm) with 80 holes of 5 aaφ was installed at the outlet of this kneader, and the kneaded material was heated to about 5 mφ.
It was made into a cylindrical pellet of x10 am.

The pellets were crushed using a crusher (Speed Mill ND-10 model) together with twice the amount (weight ratio) of 15°C cooling air.

It was introduced into Okada Seiko■). At the same time, 4 parts by weight of pulverized sodium carbonate (average particle size: 30 μm) was added as a grinding aid per 100 parts by weight of pellets.

The crusher has cutters with a length of 15 ann in four cross stages, rotates at 300 rpm, and has a screen made of 360 degree punching metal.

Three stages of this crusher are connected in succession, and the hole diameter of the screen at each stage is 3.5 rrnφ in the first stage and 2 m in the second stage.

φ, 3rd stage: 1.5m+oφ. After separating the particles from the cooling air after passing through three stages of the crusher, the average particle size of -1
The pulverized material is coated with 2% zeolite of μm, and has the composition shown in Table 1 below, and has a bulk density of 0.8 g.
/cc of detergent particles were obtained.

(2) Preparation of lipase particles Add 500 g of NaC and Ca to 100 g of various lipases.
Adding 300 g of S 0 ・2 H, and adding 100 g of a 1% aqueous solution of carboxymethyl cellulose, 2
Granulation was carried out at 00 rpm. After drying these particles in a fluidized bed to reduce the moisture content to 3%, 30g of PEG #600 and 5g of TiO□ were added.
0g was added to coat the surface.

After cooling, it was sieved to obtain lipase particles with granulation of 250 to 1000 μm and an average particle size of 500 μm.

(3) Preparation of detergent composition (high bulk density) As shown in Table-1, 100 x 1
A detergent composition was prepared by powder-mixing 0'LU/kg (to detergent) of lipase A, B, or C as lipase particles.

These were evaluated for oil stain cleaning power and the results are shown in the same table.

Details of the components used in the table are shown below.

LAS-Na: Straight-chain sodium alkylbenzenesulfonate whose alkyl group has 10 to 14 carbon atoms AS-Na: Sodium alkyl sulfate with C12/C14=7/3 Nonionic surfactant ■ Nidopanol 23 (C made by Mitsubishi Yuka Co., Ltd.)
Engineering! EOp = 20 mole adduct of Softanol 200 (Nippon Shokubai ■, 0.2-.42 alcohol); EOr3 = 20 mole adduct soap; Beef tallow soap PEG; polyethylene glycol, MV = 6000 Zeolite; Jilton B (manufactured by Mizusawa Chemical ■) A type synthetic acid zeolite carbonate-Na; Na2Go, (Reagent) Silicic acid L-
Na: JISI No. Sodium silicate sulfite - Nai sodium sulfite (reagent) PAS: Sodium polyacrylate, average molecular weight io, oo.

Protease; Savinase 4. OT (manufactured by Novo) Cellulase; Cellzyme 2400T (manufactured by Novo) Lipase A;
Lipase P (Manufactured by Jinko Pharmaceutical) Activity ratio = 100% (p)l =
9/pH=7) Lipase B; Humicala l
Lipase activity ratio = 70% (pH = 9/pH = 7) Lipase C produced from P. anuginosa Activity ratio = 20% (pH = 9/
pH=7) (blank below) Table 1 Example 2 (LAS/AS/Lipase) Detergent particles having the composition shown in Clothing-2 below were prepared by a spray drying method.

Lipase particles were powder-mixed with each detergent particle in the same manner as in Example 1 to prepare a detergent composition, and the oil stain cleaning power was evaluated.

(The following is a blank space) Table 2 Example 3 (α-3F/Nonion/Lipase) In the same manner as in Example 1, high bulk density detergent particles having the composition shown in Table 3 were prepared.

Lipase particles prepared in the same manner as in Example 1 were mixed with the powder in the amount shown in the table to obtain a cleaning composition.The oil stain cleaning power of the composition was evaluated and shown in the table.

The nonionic surfactant (■) in the table is as follows, and the other components are the same as those in Table-1.

Nonionic surfactant ■: EOβ = 20 mole adduct of Diadol 13 (Mitsubishi Kasei 0031 primary alcohol) (blank below) Table 3 ×1) Unit: 103LU/kg Detergent Example 4 (Nonionic, PEG/Lipase) High bulk density detergent particles having the composition shown in Table 4 were prepared in the same manner as in Example 1.

Lipase particles were obtained in the same manner as in Example 1 using lipase derived from Humicola 1anu (inosa).

The lipase activity of these particles was 1,900 LU/g.

Lipase particles were powder-mixed with detergent particles in the amount shown in Table 4, and the residual rate of lipase activity after long-term storage was evaluated and shown in the same table.

(Margin below) Table-4

Claims (1)

[Claims] 1. (a) Anionic surfactant: 10 to 50% by weight;
(b) A cleaning composition characterized by containing lipase. 2. (A) At least the following (A^1) and (A^2) as anionic surfactants [however, (A^1 in weight ratio)
)/(A^2)=5/1 to 1/1] and (A^1) Straight-chain alkylbenzene sulfonate: 10 to 30% by weight (A^2) The number of carbon atoms in the alkyl group is C_1_0_ to_1_
8, of which C_1_2_ to_1_4 account for 50% by weight or more of alkyl sulfates: 5 to 15% by weight (B) Alkaline lipase whose lipase activity at pH 9 is 30% or more of the lipase activity at pH 7. A cleaning composition characterized by: 3. (a) Sulfonic acid salt of a saturated fatty acid lower alkyl ester having 8 to 22 carbon atoms as a fatty acid residue: 5 to 30% by weight, and (b) ethylene oxide to a primary or secondary alcohol having 8 to 18 carbon atoms. (c) alkaline lipase whose lipase activity at pH 9 is 30% or more of the lipase activity at pH 7. Composition. 4, (α) anionic surfactant: 10 to 50% by weight,
(β) Alcohol ethoxylate prepared by adding an average of 7 to 30 moles of ethylene oxide to a primary or secondary alcohol having 8 to 18 carbon atoms; and/or polyethylene glycol having a molecular weight of 2,000 to 20,000: 2 to 10% by weight; γ) A detergent composition comprising: 0.05 to 5% by weight of alkaline lipase particles whose lipase activity at pH 9 is 30% or more of the lipase activity at pH 7.