JP2562065B2 - Enzyme detergent composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to enzyme detergent compositions. More specifically,
The present invention relates to an enzymatic detergent composition containing a lipolytic enzyme.

PRIOR ART AND DISCLOSURE The following section discusses certain publications that have been published prior to the priority date of claiming the invention, as well as certain matters that have not been published therein.

Enzymatic detergent compositions are well known in the art. While many types of enzymes have been proposed for inclusion in detergent compositions, primarily proteases and amylases have received attention. Lipase is mentioned as an enzyme that can be used in detergent compositions. Therefore, the applicant's British patent
No. 1,372,034 describes Pseudomonas stutzeri ATCC in a clothes dipping detergent composition containing a specific nonionic detergent active ingredient and optionally a specific anionic detergent active ingredient.
Disclosed is the use of a lipase produced by a microorganism of the genus Pseudomonas such as 19,154, wherein said nonionic detergent active ingredient is nonylphenol condensed with 5 or 10 moles of ethylene oxide and 3,7 or 9 moles of oxidation. A secondary alcohol condensed with ethylene is described. However, it has been found that in any and all detergent compositions, simply adding a lipolytic enzyme does not produce a satisfactory and acceptable detergent composition (as shown) with respect to enzyme activity and cleaning efficacy. It was Various components of detergent compositions have been found to have a negative effect on lipolytic enzymes.

U.S. Pat. No. 3,950,277 (Procter & Gamble) also describes clothes dipping compositions: the compositions described include lipases and lipase activators, obtained from microorganisms and other sources. A number of lipases are mentioned: those specifically mentioned as being preferred are Am
ano CE, Amano M-AP, Takeda1969-4-9, and Meito MY
Each lipase of −30, but the form in which the lipase is prepared or used is not shown.

U.S. Pat.No. 4,011,169 / NL 74,08763 (Procter & Ga
mble) describes the use of a similar range of enzymes in the preparation of detergent (detergent composition) additives.

Specific examples of known lipase-containing detergent compositions are provided by EP 0,205,208 and 0,206,390 (Unilever), which contain Ps.fluorescens in detergent compositions.
It relates to lipases related to lipases obtained from P. gladioli and Chromobacter.

European patent 0,214,761 (Novo) and European patent 0,25
No. 8,068 (Novo) provides a detailed description of lipases obtained from certain microorganisms, respectively, and also indicates certain uses of the described enzymes in detergent additives and detergent compositions. Has been done. European Patent 0,214,761 contains P
A detailed description of lipases from the organisms of the species Seudomonas cepacia, and certain uses thereof, is provided. European Patent 0,258,068 includes Thermomyces / Humicol
A detailed description of lipases from organisms of the genus a and their certain uses is given.

It also contains 5% non-ionic detergent, the rest is about 37% detergent active ingredient which is substantially anionic detergent, about 16% zeolite, about 60 LU / g + lipase protease, and other conventional detergent additives. The contained lipase-containing granular detergent composition is considered to be used in a certain field.

Another specific example of the known lipase-containing detergent composition is described in JP-A-63-078000 (1988) (Lion Corp / K. Mukoyama).
Etc.), which is based on 10-40% surfactant (eg, C ₁₄ -C ₁₈ sodium α-olefin sulfonate) and includes uses in lipase-containing systems containing other conventional detergent ingredients. Discloses the properties and uses of Pseudomonas lipase.

European Patent No. 0,268,456 (Clorox) contains Table 10 (b).
In connection with this, an experimental wash solution containing lipase and about 1 mg / ml sodium dodecyl sulfate is described.

U.S. Pat. No. 4,707,291 describes detergent compositions containing specific types of lipases. These compositions contain a mixture of anionic and nonionic detergents as the active detergent system.

Nonionic alkoxylated detergents are often present and used, and sometimes their use has been mentioned in connection with lipases.

Therefore, another specification applicable in this connection is EP 0,258,068 (Novo), EP 0,13.
0,064 (Novo), European Patent 0,206,390 (Unileve
r), U.S. Pat. No. 3,676,340 (Berg et al.-Henkel), U.S. Pat. No. 3,676,338 (Fries et al.-Henkel), West German patent 1,
942,236 (Henkel), and the following Japanese patent specifications: 63-132998 (Lion), 63-078000 (Lion),
And 63-0777998 (Hitachi).

DISCLOSURE OF THE INVENTION We have found that in certain lipase-containing detergent compositions (eg, the compositions of US Pat. No. 4,707,291 above), certain nonionic detergents, ie, low alkoxyls, more specifically defined below. It has been found that the inclusion of an alkoxylated nonionic detergent of varying degrees improves overall detergency.

The lipase used in the present invention is, for example, U.S. Pat.
Microorganisms Pseudomonas as described in 7,291
Includes lipases that show positive immunological cross-reactivity with antibodies for lipases produced by fluorescens IAM 1057. The contents of the U.S. patent specification are incorporated herein by reference.

Specific examples of lipases suitable for use in the present invention include Amano P lipase, Pseudomonas fragi FERM P 1339.
Derived lipase (sold under the trademark Amano-B), Pseudomona
s nitroreducens var.lipolyticum FERM P 1338-derived lipase (sold under the trademark Amano-CES), Chromobacter vi
scosum, such as Chromobacter viscosum var.lipolyticum
NRRLB 3673-derived lipase (commercially available from Toyo Shuzo Co., Ltd. of Tagata, Japan), and US Biochem of the United States
Chromobacter viscosum lipase and Pseudomon sold by ical Corp. and Diosynth Co. in the Netherlands
An example is lipase derived from as gladioli.

Preferred lipases are those that show a positive immunological cross-reactivity with antibodies to one of the following lipases: Chromobacter
A lipase derived from viscosum var. lipolyticum NRRLB 3673 (commercially available from Toyo Shuzo Co., Ltd., Tagata, Japan) and a lipase derived from Pseudomonas gladioli.

Typical examples of such lipases include Amano-P and Am
no-B, Amano-CES, Chromobacter viscosum, such as Chro
Lipase from mobacter viscosum var. lipolyticum NRRLB 3673 (commercially available from Toyo Shuzo Co., Ltd., Tagata, Japan), and another Chromobacter viscosum lipase (US Biochemical Corp., USA and Diosynth, The Netherlands)
(Sold by Co.), and lipase derived from Pseudomonas gladioli.

Other preferred lipases include the rDNA method (gene is a gene encoding a lipase produced by the fungus Humicola lanuginosa, which is the host Aspergillus or
lipase produced by cloning the gene in yzae). A particularly preferred lipase is the trademark of the lipolase Lipolase, Novo I of Denmark.
It is manufactured and sold by ndustri A / S (Bio
See Technology Newswatch, March 7, 1988, page 6.)

A lipase immunologically identical or similar to such a lipase may be used in the present invention.

Further suitable lipases are for example: eg the following patent specifications, EP 0,214,761 (Novo), EP
0,258,068 (Novo), and in particular The
Lipases showing immunocrossreactivity with antisera raised against lipases derived from rmomyces lanuginosus ATCC 22070 (European Patent No. 0205,208 (Unilever) and European Patent No.
0,206,390 (Unilever)): and especially Alcaligenes P
Lipases showing immunocrossreactivity with antisera raised against lipases obtained from L-679, ATCC 31371 and FERM-P 3783, and further WO 87/00859 (Gist-Brocade
s) and EP 0,204,284 (Sapporo Breweries). Particularly suitable is, for example, another commercially available lipase preparation, for example: Amano lipase CE, AP,
M-AP, AML, and Meito lipase MY-30, OF, and PL, as well as esterase MM, lipozyme Lipozym, SP 225, SP 285, Sai
ken lipase, as well as Enzeco Lipase ™.

A suitable lipase gene, eg Thermomyces lanugino
Genetic engineering of this enzyme can be carried out by extracting a gene for lipase from sus or a mutant strain thereof, and introducing and expressing the gene or a derivative thereof into an appropriate producing organism such as Aspergillus. WO 88/02775 (Novo), E
P 0,238,023 (Novo), EP 0,243,338 (Labofin
The method described in a) and EP 0,268,452 (Genencor) can be applied and applied. Such an enzyme can be produced by the respective ancestral organisms even if the secondary characteristics of the enzymatic material, such as the degree of glycosylation, differ between the ancestral and productive products. Can be called an enzyme. All the above cited specifications are
Included herein by reference.

Particularly suitable lipases are eg those described in EP 0,305,216.
No. (Novo). The description of the European patent specification is incorporated herein by reference.

The presently preferred lipase is the lipolase Lipolase.
(Novo trademark), and lipases obtained from Pseudomonas gladioli or their rDNA-derived lipases.

The lipase of the present invention has a final detergent composition of 100-0.005 LU.
/ Mg of composition, preferably in an amount such that it has a lipolytic enzyme activity of 25-0.05 LU / mg in the detergent composition.

In certain cases, within a wide range, for example from 50 to 30,000 LU per gram of granular detergent composition, for example often at least 100 LU / g, very usually at least 500 LU / g,
Sometimes preferably 1000 LU / g or more, 2000 LU / g or more, or
Above 4000 LU / g, and therefore very often within the range of 50-4000 LU / g, and probably within the range of 200-1000 LU / g,
The addition amount of the lipolytic enzyme can be selected.

The lipase unit (LU) was measured by a pH stat per minute under the following conditions (temperature 30 ° C., pH = 9.0; substrate was 3.3 mmol in the presence of 13 mmol Ca ²⁺ and 20 mmol NaCl in 5 mmol Tris buffer).
The amount of lipase that produces 1 μmol of titratable fatty acid with an emulsion of wt% olive oil and 3.3% gum arabic).

Of course, mixtures of the above lipases can be used. The lipase can be used in unpurified form or in purified form, for example by purification by well known adsorption methods such as the phenyl sepharose packed column method. Lipase as a granular composition of a lipolytic enzyme with a carrier substance (see eg EP 0,
No. 258,068), or as a slurry, can be conveniently added.

The lipase-introduced detergent composition contains, as active detergent substance, a mixture of one or more nonionic synthetic detergent active substances and one or more anionic synthetic detergent active substances.

Anionic detergent actives are well known in the art and suitable examples are given by Schwartz, Perry and Berch, "Surfactants and Detergents", Volume I (1949) and Volume II (19).
58), and Schick, Nonionic Surfactants, Vol. 1 (1967).

Anionic detergent actives usually have about 8 to 22 carbon atoms.
Water-soluble alkali metal salts of organic sulphates and sulphonates having alkyl groups, the term alkyl being used including the alkyl part of the higher acyl groups. Examples of suitable synthetic anionic detergent compounds, alkyl sulfates, sodium and potassium, higher (C ₈ ~C ₁₈₎ generated from the particular example tallow or coconut oil as obtained by sulfating the alcohols; Alkyl (C ₉ -C ₂₀ ) benzene sulfonates of sodium and potassium, especially straight chain secondary alcohol (C ₁₀ -C ₁₅ ) benzene sulfonates of sodium; Alkyl glyceryl ether sulfate (especially ethers thereof) coconut oil fatty monoglyceride sulfates and sulfonates of sodium; higher alcohols and ethers of synthetic alcohols derived from petroleum) obtained from tallow or coconut oil is higher (C ₈ -C ₁₈₎ fatty alcohol - alkylene oxides (especially ethylene oxide Reaction product) Sulfate sodium and potassium Reaction products of fatty acids such as coconut fatty acid esterified with isethionic acid and neutralized with sodium hydroxide; sodium and potassium salts of fatty acid amides of methyl taurine; α-olefins (C ₈ -C ₂₀ ). With alkane monosulfonates such as those obtained by the reaction of bisulfite with sodium bisulfite, and as obtained by reacting paraffins with SO ₂ and Cl ₂ and then hydrolyzing with bases to form the sulfonates. Alkane monosulfonates; as well as olefin sulfonates, the term olefin sulfonate is used to react an olefin, in particular a C _{10 to} C ₂₀ α-olefin with SO ₃ and then to react the reaction product It is used to indicate the substance formed by neutralization and hydrolysis. A preferred anionic detergent composition is
(C ₁₁ ~C ₁₅₎ sodium alkylbenzenesulfonate, and (C ₁₆ ~C ₁₈₎ is a sodium alkyl sulfate.

Nonionic detergent actives are generally low alkoxylation of fatty compounds selected from fatty alcohols, fatty acids, fatty esters, fatty amides and fatty amines, to the extent of at least 30% by weight of the total nonionic detergent actives. A nonionic detergent active that is a degree alkoxylate adduct. The fatty compound contains at least 10 carbon atoms and the nonionic substance has an average of less than 5 alkylene oxide groups per molecule, eg less than 4 alkylene oxide groups per molecule, eg 3.5 per molecule, Effectively has 3 or less alkylene oxide groups,
And, friendly, it contains 0.5 or 1 or 2 alkylene oxide groups per molecule.

The alkylene oxide residue may be, for example, an ethylene or propylene oxide residue.

Accordingly, the alkylene oxide adducts of fatty alcohols useful in the present invention have the general _{formula: R-O- (C n H} 2n O) in _y H (wherein, R is at least 10 carbon atoms, and most preferably 10 to carbon atoms 22 alkyl or alkenyl groups, y is preferably about 0.5-3.5, and n is 2 or 3). As preferably and suitable examples of such materials are C ₁₃ -C ₁₅ alcohol having about 3 ethylene oxide groups per molecule Synper
onic A3 (ICI) and E, which is lauric alcohol 3EO
Examples include mpilan KB3 (Marchon).

The alkylene oxide adducts of fatty acids useful in the present invention preferably have the general formula: Where R, n and y are the same as above. A suitable embodiment is the tallow fatty acid ESONAL 0334 (Dia) which has about 2.4 ethylene oxide groups per molecule.
mond Shamrock).

Alkylene oxide adducts of fatty esters useful in the present invention include mono-, di-, or tri-esters of polyhydric alcohols having 1 to 4 carbon atoms such as coconut oil or tallow oil (triglyceride) 3EO (Stearine Dubois). Examples include adducts.

The alkylene oxide adducts of fatty amides useful in the present invention preferably have the general formula: (In the formula, R is an alkyl or alkenyl group having at least 10 carbon atoms, most preferably 10 to 22 carbon atoms, n is 2 or 3, and x and z are 4.0 or less in total, preferably about
0.5-3.5, where either x and z can be zero). Specific examples of such substances include coconut oil monoethanolamide and diethanolamide,
And the corresponding tallow and soy compounds.

The alkylene oxide adducts of fatty amines useful in the present invention preferably have the general formula: (Wherein R and n are the same as above, x and z are preferably about 4.0 or less in total, and most preferably about 0.5 to 3.5.
Is). Specific examples of such substances include:
EthomeenT 12 (tallow amine 2EO, sold by AKZO), Optamee
nPC5 (coconut oil alkylamine 5EO), and Crodamet
(1.02 (oleylamine 2EO), sold by Croda Chemical).

One useful criterion of choice for nonionic surfactants for use in certain preferred embodiments of the present invention is that the cloudy p is somewhere in the temperature range of 0 ° C to 40 ° C.
hase) (1% w / w in distilled water).

Preferably, a selection of nonionics having an HLB value of about 5-10.5, for example about 7-9.

The weight ratio of the low alkoxylation degree nonionic of the anionic detergent is preferably less than 1: 1 and usually less than 1: 2, for example 1: 1 to 1: 3 or 1: 1 to 1: 4. Range, often in the range 1: 2.4 to 1: 3.

The combined amount of the nonionic detergent active substance and the anionic detergent active substance in the detergent composition is in the range of 1 to 30% by weight,
Very often it can range up to 25% by weight, usually 2 to 20% by weight, and often 6 to 16% by weight. Some preferred examples have total surfactant in the range of 10-20% by weight.

It may also contain other cleaning substances such as soaps, cationic and zwitterionic or zwitterionic detergents, for example amine oxides.

Further, the detergent composition may include conventional detergent ingredients in conventional amounts. They may or may not contain a builder, and may be of the Zero-P type (ie containing no phosphorus-containing builder).

Therefore, the composition is 1 to 45% by weight, preferably 5
It may contain up to 30% by weight of one or more organic and / or inorganic builders. Typical examples of such builders include alkali metal ortho-, pyro-, and tri-
Examples thereof include polyphosphates, alkali metal carbonates, alone or in a mixture with calcite, alkali metal citrates, alkali metal nitrilotriacetates, carboxymethyloxysuccinates, zeolites and polyacetal carboxylates. In addition, it may contain from 1 to 35% of a bleaching agent, or a bleaching system consisting of a bleaching agent and its activator, for example sodium perborate + TAED. For example, DPDA (= diperoxydodecanedioic aci
d)), or for example European Patent 0,271,152 (Unilever)
It is also possible to use more powerful bleaching systems such as sodium perborate with bleach precursors that produce peracid faster than with TAED as described in.

Preferred for many applications, and particularly beneficial in terms of detergent performance in the presence of lipase, are phosphorus-free and substantially phosphorus-free builder compositions (eg, less than about 1% thereof). is there.

Furthermore, the composition is a foam booster, foam control agent, corrosion inhibitor, sewage suspension agent, softener, clay, sequestering agent, antifouling agent, as disclosed in our European Patent No. 0003172. Perfumes including various fragrances, dyes, stabilizers for enzymes and the like may be contained. They may contain enzymes other than lipases, such as proteases, amylases, oxidases and cellulases.

Specific examples of other components include a polymer that can be composed of a homopolymerized and / or copolymerized carboxylic acid or a sulfonate, or a sodium or potassium salt thereof, and a sodium salt is preferable. Suitable homopolymers are polyacrylic acid, polymethacrylic acid, polymaleic acid and polystyrene sulphonic acid. Suitable copolymers include copolymers of acrylic acid, methacrylic acid and maleic acid with vinyl ethers such as vinyl acetate or vinyl propionate, acrylamides, methacrylamides, and ethylene, propylene or stellene or styrene sulfonates. Is mentioned. In copolymerized acids where one of the components does not contain an acid functional group, the content of this component is below 70 mol%, preferably below 60% mol in order to obtain sufficient water solubility. For example, European Patent No. 25,551-B1, and Tenside 197.
Copolymers of acrylic acid and maleic acid, as further characterized in 9, 16 , 82-89, have been found to be particularly suitable. These are copolymers containing 40 to 90% by weight acrylic or methacrylic acid and 60 to 10% by weight maleic acid. Copolymers of this kind contain 45 to 85% by weight of maleic acid. Copolymers of this type which contain 45 to 85% by weight of acrylic acid and 55 to 15% by weight of maleic acid are particularly preferred. The molecular weight of homopolymers and copolymers is generally 1000 to 150,000, preferably 1500 to 100,000.
Is.

Other suitable polymeric materials include cellulose ethers such as carboxymethyl cellulose, methyl cellulose, hydroxyalkyl cellulose, and mixed ethers such as methyl hydroxyethyl cellulose, methyl hydroxypropyl cellulose, and methyl carboxymethyl cellulose. Mixtures of different cellulose ethers, especially mixtures of carboxymethylcellulose and methylcellulose, are suitable. Molecular weight 400-50,000,
Preferably 1,000 to 10,000 polyethylene glycol,
Also, copolymers of polyethylene oxide and polypropylene oxide are suitable, as are copolymers of polyacrylates and polyethylene glycol. Molecular weight 10,000-60,0
Suitable are 00, preferably 30,000 to 50,000 polyvinylpyrrolidone and copolymers of polyvinylpyrrolidone with other polypyrrolidones. Also suitable are polyacrylic phosphonates and related copolymers having a molecular weight of 1,000 to 100,000, in particular 3,000 to 30,000.

The compositions of the present invention may be formulated in any desired form such as powders, rods, pastes, liquids and the like. For example, when dissolved or dispersed in water to give a surfactant concentration of about 0.8 g /
Very often, the composition can produce a wash liquor at a pH of about 7-10.5, such as a pH of about 9-10.

For example, the detergents of the present invention have a bulk density of at least 600 g / m and are in the form of granules with sufficiently low or zero neutral inorganic salts (eg sodium sulfate), phosphate or aluminosilicate builders and minor amounts. When the composition is dispersed in water to make a washing liquid having a surfactant concentration of about 0.8 g /, it has an ionic strength of about 0.04 or less, for example about 0.03 or less, for example about 0.025 or 0.02 or less. Produces a wash liquor.

Further, the detergent liquid of the present invention is, for example, EP 0,266,19.
It may be formulated as a substantially anhydrous liquid detergent composition comprising a solution such as a dispersion formulated as in No. 9 (the contents of which are incorporated herein by reference).

 The invention is further described by the following examples.

A composition in which the nonionic surfactant consists of the 7EO material only is a comparative composition.

Example 1 A test was carried out with the following composition: In Compositions A and D, nonionic detergents with a degree of alkoxylation of 3 accounted for 75% of the total nonionic detergents and 25% of the total of the anionic detergents and the low alkoxylated nonionic detergents.

A washing experiment was conducted under the following conditions: Water hardness: 27 ° FH Test monitor: Prewashed cotton soiled with a mixture of inorganic pigment, protein and peanut oil Washing program: heating at 30 ° C for 5 minutes, test monitor and washing Wash with ballast load (C / L ratio 1:10) at 30 ° C for 30 minutes, then rinse 3 times with tap water.

Amount of detergent: 5g / Lipase used: Lipolase (Novo) Lipase (from Ps.gladioli) Lipase MY (Meito) Lipase AP-6 (Amano) Lipase Dose: 0.5,1,3 or 15LU / washing solution 1ml After a fourth soil / wash cycle, the reflectance of the test fabric and the percent residual fatty material on the test fabric were measured. The reflectance was measured with a reflectometer at 460 nm using a UV filter in the optical path, and the fatty substance was measured by extracting the dried test cloth with petroleum ether, distilling off the solvent, and weighing the resulting fatty substance.

The following results were obtained: Example 2 Similar to composition A, but containing 6% anionic material, 4% nonionic material (7EO) and 2.5% nonionic material (3EO), 1.5% by weight Lipolase 30 T and carbonate / sulfate. Double salt
The composition containing 8.5% produced results similar to composition A of Example 1.

In the composition of Example 2, the low alkoxylation degree (3) nonionic detergent accounted for about 38.5% of the total nonionic components, and about 2% of the total of the anionic detergent and the low alkoxylation nonionic components.
It accounts for 9.4%.

Example 3 The detergent powder composition of this example has a proportion of anionic detergent.
Similar to composition D of Example 1 except that the proportion of 12%, 7EO-nonionic detergent was 1% and the proportion of 3EO-nonionic detergent was 4%. There is 0.45% Savinase 4.0T protease. Use Lipolase 100T at a rate of 0.5%.

 Adjust the balance of neutral inorganic salts accordingly.

This embodiment is a very preferred embodiment of the present invention,
It shows unexpectedly excellent washing performance.

In this composition, 80% of the total nonionic detergent in which the 3EO-nonionic material is present, and 25% of the total of the anionic component and the low alkoxylated nonionic component.

The scope of the specification and claims extends to all modifications and variations, including combinations and subcombinations of the features.

Continuation of front page (56) References JP-A-61-285295 (JP, A) JP-A-61-272299 (JP, A) JP-A-61-252300 (JP, A) British patent 1372034 (GB, A)

Claims (10)

(57) [Claims] 1. A detergent composition comprising an anionic surfactant, a nonionic surfactant and a lipase enzyme, wherein (a) the nonionic surfactant of the composition is at least C
Low-alkoxylated nonionic interface selected from fatty alcohols, fatty acids, fatty esters, fatty amides and alkoxylate adducts of fatty amines having a chain length of ₁₀ and an average alkylene oxide content of less than 5 alkylene oxide groups per molecule. A low-alkoxylated nonionic surfactant component comprising from 30 to 80% by weight of the total nonionic surfactants of the composition; and (b) a nonionic surfactant and an anion in the composition. The total amount of the surfactant is 1 to 30% by weight; (c) the low alkoxylated nonionic surfactant component is less than 50% by weight of the total of the low alkoxylated nonionic surfactant component and the anionic surfactant. (D) The lipase enzyme is about 0.005 to 1 mg of the detergent composition.
Present in an amount of 100 LU; 2. A detergent composition according to claim 1 wherein the ratio of low alkoxylated nonionic surfactant component to anionic surfactant is in the range of 1: 1 to 1: 4. 3. The detergent composition according to claim 1, wherein the surfactant content is in the range of up to 20% by weight. 4. Lipase is used for Humicola lanuginosa, Pseudo
monas gladioli, or Chromobacter viscosum var lipol
The detergent composition according to any one of claims 1 to 3, which is selected from lipases that can be produced by yticum. 5. Lipase is used as Chromobacter viscosum var
Pseudomonas glad, a lipase that shows a positive immunological cross-reactivity with an antibody against lipolyticum NRRLB 3673-derived lipase
5. A lipase that shows a positive immunological cross-reactivity with an antibody to lipase derived from ioli, and a lipase that shows a positive immunological cross-reactivity with an antibody to a lipase derived from Humicola lanuginosa, selected from any one of claims 1 to 4. The detergent composition according to. 6. The detergent composition according to claim 5, wherein the lipase is Lipolase (registered trademark). 7. A nonionic surfactant alkoxylate adduct is a C ₁₀ -C ₂₂ fatty alkyl, alkenyl having an average degree of alkoxylation in the range of 0.5 to 3.5 ethylene oxide groups and / or propylene oxide groups per molecule. , alkanoyl and alkenoyl alkoxylates, and C ₁₀ -C ₂₂ fatty alkyl amines having 0.5 to 3.5 or an average degree of alkoxylation in the range of ethylene oxide and / or propylene oxide groups per molecule, alkenyl amines, alkanoamides and Arukenoamido 7. Detergent composition according to any one of claims 1 to 6, characterized in that it is selected from N-mono-alkoxylates and N, N-dialkoxylates. 8. A builder selected from zeolite, calcite, alkali metal carbonates, citrates, nitrilotriacetic acid salts, carboxymethyl succinic acid salts and polyacetal carboxylic acid salts in an amount of 1 to 45% by weight, and substantially The detergent composition according to any one of claims 1 to 7, which does not contain a phosphorus-containing builder compound. 9. The detergent composition according to claim 1, which is in the form of powder. 10. The detergent composition according to any one of claims 1 to 8, which is in a liquid form.