JPH02215706A - Antimicrobial composition and cosmetic - Google Patents

Abstract

PURPOSE:To obtain an antimicrobial composition suitable for cosmetic compounding a reduced amount of paraben, having low irritation and safety without lowering antiseptic, antimicrobial and sterilizing effects by blending paraben with pionin. CONSTITUTION:An antimicrobial composition and cosmetic having low irritation containing pionin and paraben. The blending ratio is preferably 0.001-0.005wt.% pionin and 0.02-0.1wt.% paraben. The amount of paraben blended can be reduced to about 1/10, the composition or cosmetic has no problems of solubility and yet has antimicrobial power. Use of both the components shows sufficiently antimicrobial and sterilizing effects on cosmetic contaminating microorganisms.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to hypoallergenic antimicrobial compositions and cosmetics containing pionin and parabens.

BACKGROUND OF THE INVENTION Cosmetics used for the skin, such as cosmetics and quasi-drugs, are often designed to be easily blended into the skin. Therefore, microorganisms are likely to grow, and damage caused by cosmetics contaminated with microorganisms has been reported. Such cosmetics are composite agents of many compounds, and as a recent phenomenon, natural extracts from many plants and animals are included as useful ingredients, and as a base, they provide a favorable environment for the growth of microorganisms. ing.

Cosmetics usually contain preservatives and antibacterial agents to prevent this contamination. Preservatives and antibacterial agents have cytotoxic effects and exhibit the effect of suppressing the proliferation of microorganisms (bacteriostatic effect) or killing them (sterilizing effect). However, these preservatives and antibacterial agents have problems such as inducing side effects such as contact dermatitis, photocontact dermatitis, allergies, and photoallergies on the skin. Of course, there are concerns about side effects not only in preservatives and antibacterial agents, but also in other cosmetic ingredients, and as one of the safety measures for cosmetics, the Ministry of Health and Welfare recommends that ingredients with potential side effects be labeled in the product container. There is an obligation to display the information. (
(Ministry of Health and Welfare Public Notice No. 125 of 1980) This list includes preservatives and antibacterial agents used in cosmetics such as parabens, sodium benzoate, isopropylmethylphenol, undecylenic acid, benzalkonium chloride, dehydroacetic acid, and resorcinol. are all listed.

In particular, parabens, which are typical preservatives and antibacterial agents widely used in cosmetics and quasi-drugs, are 02-2.
Although it has the expected effects at 0%, many drawbacks have been reported, such as poor solubility in the base, paraben allergy, and skin damage caused by parabens. Reducing the amount added would ensure safety and solubility, but the antibacterial effect would be extremely reduced, so currently we have no choice but to use it at the current concentration.

For these reasons, there is currently no ideal preservative/antibacterial agent, and as described above, the maximum allowable amount of the preservative/antibacterial agent is often used.

Problems to be Solved by the Invention However, cosmetics such as cosmetics and quasi-drugs are economically viable for long-term use. Furthermore, since cosmetics are composite agents of various compounds, their antiseptic and antibacterial effects are often significantly reduced by various factors. For example, there are effects of pH, oil/water partition ratio, adsorption to surfactants and polymer compounds, etc. In addition, microorganisms and carbon sources, nitrogen sources, salts, vitamins, etc. used as growth factors, etc. Provides optimal conditions.

For these reasons, it is essential to select antiseptic and antibacterial agents that are safe in small amounts and effective against a wide range of microorganisms. Therefore, instead of relying on a single compound, efforts are being made to reduce the amount by combining two to three types according to the base conditions and to satisfy safety and effectiveness.

The ideal antiseptic/antibacterial agent should (1) have a broad antibacterial spectrum, (2) be harmful in small amounts, (3) be sufficiently soluble, and (4) be as safe as possible, non-toxic and non-irritating to living organisms. Examples include things that are expensive.

A major challenge in the industry is to create safer formulations by reducing the amount of parabens, which are the most commonly used substances, without sacrificing their antiseptic and antibacterial effects.

Means for Solving the Problems In order to solve this problem, the present inventors reduced the amount of parabens blended and tested substances that exhibit additive or synergistic effects with parabens. Cosmetics and other substances that come into direct contact with the skin must be safe and must be approved for inclusion in cosmetics.

Therefore, a lot of research has been conducted not only in terms of effectiveness but also in terms of safety.
We focused on pionin, which is no longer required to be labeled as a compounded substance in cosmetics due to its safety, and attempted to use it in combination.

Pionin (Photosensor No. 201) is widely used as a special ingredient in cosmetics at 0.002% or less (0.005% or less as a quasi-drug), and is highly safe for the skin.
It is a raw material that is not required to display its ingredients. Pionin is used as a preservative aid to prevent microbial contamination (primary contamination) during the production process of cosmetics, but it is not used as a disinfectant during cosmetic use (secondary contamination).

The present inventors added 0.001 to 0.005% by weight of pionin.
The present invention was developed based on the discovery that by adding parabens, the concentration of parabens used in combination can be greatly reduced to 1/10 of the conventional amount without reducing the antiseptic and antibacterial effects during storage and use. completed.

The present invention has made it possible to reduce the amount of parabens, which has been an issue in the industry.

Here, "cosmetics" refers to those stipulated in Class 4 of the Trademark Law Enforcement Regulations (soaps, toothpaste, cosmetics, etc.).

That is, the present invention relates to hypoallergenic antimicrobial compositions and cosmetics characterized by containing pionin and parabens.

The hypoallergenic composition for microorganisms according to the present invention can be used in makeup cosmetics. ointment. cream. lotion. Emulsion. pack. Basic cosmetics such as soap, contact lens cleaning agents. Same preservative. It can be used as an antiperspirant spray.

Function The present invention is a two-drug combination product containing 0.001 to 0.005% by weight of pionin and 0.02 to 0.1% of paraben.

Pionio dissolves in most bases at this extremely small concentration, does not cause any abnormalities due to contact sensitization or photosensitization, and causes very slight irritation to the mucous membranes of the eye.The pH range is from weakly acidic to mildly acidic, which has no effect on living organisms. It is stable in alkaline conditions and stable in various surfactants, none of which show any loss of efficacy. It also has a broad antibacterial spectrum against both Gram-positive and Gram-negative bacteria. (Journal of the Japanese Cosmetic Science Society 8 (No. 1)
). P43.1984) On the other hand, parabens are used in the currently commonly used amount of 0.1 to 1.
At a concentration of 0%, it may be difficult to dissolve or even insoluble in cosmetic bases.

Therefore, attempts have been made to tilt the oil-water distribution ratio toward the oil side or to use surfactants to form and dissolve micelles. With fins, the original antiseptic and antibacterial effects cannot be expected, and the formulation of the base requires some ingenuity or two.

Furthermore, parabens are transdermal absorbable (transdermal absorbable) and can easily cause sensitization and contact dermatitis, which suggests the dangers of fat-soluble formulations.

By reducing the amount of paraben added to about 1/10, there is no problem with solubility and antibacterial activity can be expected depending on the added concentration.

As shown in the attached chart, the combined use of these two products provides sufficient protection against Staphylococcus, Bacillus subtilis, Streptococcus, Pseudomonas aeruginosa, Escherichia coli, etc., which are commonly detected as cosmetic contaminants. Sterilizing power was obtained.

In addition, abnormalities in the bacterial flora on the skin, such as resident bacteria and temporary bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, etc.), are the most likely to cause skin damage, and if the injury is exceeded, secondary infections (Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, etc.) (Pseudomonas aeruginosa, P. acnes, etc.).

It is important for cosmetics to cleanse the skin and to prevent abnormalities in the skin flora. By the way, pionin has antibacterial properties against acne-causing bacteria, P. acnes, and may also have an acne-healing effect (
Japan Cosmetic Science Society Poetry Journal 7 (NO.3) P250.198
3) Incorporation into cosmetics is also useful in this sense.

Next, embodiments and effects of the present invention will be described in more detail.

Examples Example 1 20ml of lotion of method 1 in a sterilized sample bottle
1 of Pseudomonas aeruginosa and pre-cultured cosmetic-derived Pseudomonas aeruginosa solution.
．． 2 ml (107/ml) was added, thoroughly mixed with a vortex mixer, and stored in a thermostat at 30°C. A certain amount of this sample was collected every day, and a surfactant (Twee×n8
0) After diluting with phosphate buffer, spread the diluted solution on a heart infusion agar medium and incubate at 37℃ for 2 hours.
Culture was carried out for 4 hours, and the resulting colonies were counted and weighed 1 g (1
The effectiveness was determined based on the number of viable bacteria per ml.

As shown in Figure 1, when pionin and paraben are added alone, the antibacterial effect is weak or almost non-existent, but when the combined addition composition of pionin and paraben at the same concentration according to the present invention is used, the antibacterial effect is observed after 24 hours. The flora showed excellent antibacterial activity, which was not observed at all.

This shows that the two agents have an excellent synergistic effect against a large number of Pseudomonas aeruginosa bacteria (107 starting bacteria) at concentrations that do not exhibit antibacterial activity when used alone. It means to have.

Example 2 Pre-cultured cosmetic-derived Escherichia coli solution was prepared into lotion (emulsion) according to method 2 so that the number of starting bacteria was 106/ml.
In addition, tests were conducted in the same manner as in Experimental Example 1. As shown in Table 1, the combination composition according to the present invention also showed excellent effects against E. coli.

Example 3 A pre-cultured cosmetic-derived Pseudomonas aeruginosa liquid was added to the cream of Preparation 3 so that the number of starting seedlings was 107/ml,
A static culture was performed and tested in the same manner as in Example 1. The conclusion is shown in Figure 2.

Even with this base composition, the effectiveness of the combination of the two drugs was sufficiently observed.

Example 4 A pre-cultured Pseudomonas aeruginosa liquid derived from cosmetics was prepared to have an initial bacterial count of 107/ml, added to the lotion in Step 4, and subjected to static culture in the same manner as in Example 1. . The results are shown in FIG.

This lotion is mostly water, but natural extracts are added as humectants.

Is this an effect, or is it a solubility problem because most of the base is water?
Although the efficacy tends to be slightly lower than that of Examples 1 to 3, it shows the additive effect of the two drugs of the present invention.

Example 5 Pre-cultivated Bacillus subtilis solution derived from cosmetics was made into a lotion (emulsion) according to Preparation 5 so that the number of starting bacteria was 106/ml.
In addition, tests were conducted in the same manner as in Example 1.

Here, Bacillus subtilis is widely distributed in the natural world such as dried grass, soil, and dust, and therefore exists in large numbers as an airborne bacterium.

Moreover, it is said to be a highly resistant bacterium that has spores.

As a result of this example, paraben alone has weak antibacterial activity and does not exhibit antibacterial effects at the low concentrations used in the present invention, but pionin alone exhibits antibacterial effects at concentrations of approximately 0.003 to 0.005%. . Furthermore, in the two-drug combination according to the present invention, 0.001 to 0.002% of pionin and 0.03 to 0.0% of paraben are used.
It showed sufficient antibacterial activity at extremely low concentrations of 2% (Figure 4).

Example 6 Exposure test for airborne bacteria When examining the general susceptibility of preservatives and antibacterial agents, a method is used to observe the growth and development of fallen bacteria on a flat plate to which the target agent has been added.

Regarding the product of Method 6, 20g (ml) of it was placed in a sterilized petri dish, exposed indoors for 5 hours, loaded with airborne bacteria, and then placed in a 30°C incubator for one week (7 days).
A certain amount was taken and cultured on a heart infusion agar plate medium.

As a result, bacterial colonies were observed in all the additive-free groups that did not contain the composition of the present invention. The breakdown was spore-forming bacteria, gram-negative bacteria, gram-positive bacteria, and molds. In contrast, no bacterial colonies were observed in any of the test products containing pionin and paraben of the present invention.

The treatment method will be explained below.

Recipe 1 Rosin Oil-based component Microcrystalline wax 1.0% by weight Beeswax 2.0 Lanolin
2.0 Liquid paraffin 30.0 Emulsifier Sorbitan sesquioleate 4.0 Polyoxyethylene sorbitan monooleate (20E.O.
) 1.0 Aluminum stearate 0.2 Fragrance 0.4 Preservative/antibacterial agent Composition of the present invention Humectant Glycerin 8.0 Purified water 51.4 Preparation method 2 Lotion Oily component Stearic acid 2.0
% Setanol 1.5 Vaseline 3.0 Lanolin alcohol 2.0 Liquid paraffin 10.0 Emulsifier Polyoxyethylene monooleate (10
E. O. ) 2.0 Fragrance 0.5 Preservative/antibacterial agent
Composition of the present invention Moisturizer Glycerin 3.0 Propylene glycol 5.0 Alkali Triethanolamine 1.0 Purified water 70.0 Method 3 Cold cream Oily component Beonyl alcohol 3.0% Beeswax 4.0 Paraffin wax 2.5 Liquid paraffin 48.0 Fatty acid glyceride 2.0 Emulsifier Polyethylene glycol fatty acid ester 0.5 Glycerin fatty acid ester 1.
0 Polyoxyethylene sorbitan monolaurate (20E.O.) 2.5 Polyoxyethylene alkyl ether 1.0 Borax 0.3 Fragrance 0.
2 Preservative/antibacterial agent Composition of the present invention Moisturizer Hyaluronic extract 0.5 Purified water 34.5 Prescription 4 Lotion
Ethanol 3.0 Detergent Polyoxyethylene lauryl ether 0.3 Humectant Glycerin 0.5
Sodium pyrrolidone carboxylate 1.0 Buffer
Citric acid 0.1 Sodium citrate 0.2 Flavoring agent Appropriate amount Wetting agent Bifidobacteria extract 1.0 Hyaluronic extract 0.5 Preservative/antibacterial agent Composition of the present invention Purified water 93.4 Prescription 5 Lotion Oily component Squalane 5. 0%
Vaseline 2.0 Beeswax 0.5 Lanolin alcohol 1.5 Stearic acid 0.3 Emulsifier Sorbitan sesquioleate 0.8 Polyoxyethylene oleyl ether (20E.O.) 1.2
Fragrance 0.2 Preservative/antibacterial agent Composition of the present invention Humectant Glycerin 2.0 Probylene glycol 3.0
Thickener Carboxyvinyl polymer 0.0 Purified water 83.4 Prescription 6 Contact lens cleaning solution Pullulan 2.0%
Polyoxyethylene lauryl ether 0.5 Sodium chloride 0.6 Preservative/antibacterial agent Composition of the present invention
Purified water 96.9 Effects Parabens are currently most commonly used as preservatives and antibacterial agents in the cosmetics field, but as mentioned above, they have many drawbacks in the amounts currently used, such as dissolution, skin irritation, and There are many problems with transdermal absorption into living organisms.

Pionin is a very small amount of 0.002 to 0.005%, has an antibacterial effect, is a low toxicity and highly safe compound.

The present invention uses these two compounds in combination to significantly reduce the amount of parabens added, greatly increasing the safety of cosmetics and other products, and improving anti-microbial measures during the manufacturing process and storage of cosmetics. This has made it possible to greatly contribute to improving product stability.

Brief Description of the Drawings Figure 1 Antibacterial effect of the composition of the present invention and the comparative agent against Pseudomonas aeruginosa in the lotion of Preparation 1 A; Composition of the present invention (0.005% pionin, 0.05% paraben) B ;
Composition of the present invention (pionin 0.004%, paraben 0.0
7%) C: Composition of the present invention (Pionin 0.003%, Paraben 0.1%) D: Comparative agent (no addition) E: Comparative agent (paraben 0.05%) F: Comparative agent (Pionin 0.0
05%) G: Comparative agent (paraben 0.1%) Figure 2 Antibacterial effects of the composition of the present invention and the comparative agent against Pseudomonas aeruginosa in the cream of Prescription 3 A: Composition of the present invention (paraben 0.1%)
．． 005%, paraben 0.02%) B; Composition of the present invention (pionin 0.002%, paraben 0.05%) C;
Composition of the present invention (pionin 0.002%, paraben 0.0
7%) D: Composition of the present invention (Pionin 0.002%, Paraben 0.03%) E: Comparative agent (no addition) F: Comparative agent (paraben 0.1%) G: Comparative agent (Pionin 0.0
05%) Fig. 3 Antibacterial effects of the composition of the present invention and comparative agents against Pseudomonas aeruginosa in cosmetics of Method 4 A: Composition of the present invention (0.002% pionin, 0.1% paraben) B: Main Invention composition (0.005% pionin, 0.07% paraben)
C: Composition of the present invention (0.003% pionin, 0.07% paraben) D: Comparative agent (no addition) E: Comparative agent (0.002% pionin) F: Comparative agent (0.05 paraben
%) G: Comparative agent (0.005% pionin) Figure 4 Antibacterial effects of the composition of the present invention and the comparative agent against Pseudomonas aeruginosa in the lotion of Prescription 5 A: Composition of the present invention (0.002% pionin) , paraben 0.02%) B; Composition of the present invention (pionin 0.001%, paraben 0.03%) C
; Comparative agent (Pionin 0.003%) D; Comparative agent (no additive) E; Comparative agent (paraben 0.05%) F; Comparative agent (paraben 0.1%)

Claims (3)

[Claims] (1) Hypoallergenic antimicrobial composition characterized by containing pionin and parabens (2) The composition according to claim 1, which contains 0.001 to 0.005% by weight of pionin and 0.002 to 0.1% by weight of parabens. (3) Cosmetics characterized by containing pionin and parabens