RU2409367C2 - Prophylactic agent for skin with antituberculous effect - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to prophylactic agent for skin, having antituberculous effect, which includes the following active substances - 0.50-0.75 wt % of streptomycin and 7.0-10.0 wt % of stabilised sol of silver nanoparticles, and also polyethylene oxides (PEO) of brands 400 and 1500 as the base.

EFFECT: invention provides a synergetic effect, which shows in ability to suppress antibiotic-resistant strains of microorganisms, including mycobacteria of tuberculosis, and in improvement of bactericide properties of proposed agent.

1 tbl, 5 ex

Description

The invention relates to the field of cosmetics, and can also be used in the manufacture of a prophylactic for the skin, which, due to the combination of active substances contained in it, has an antiseptic effect on a wide range of pathogenic microorganisms, including mycobacteria that cause skin tuberculosis.

Currently, a wide variety of antiseptic agents for the skin with a diverse formulation is known. All components of such funds can be divided into the following main groups:

- basic (oil / water, higher fatty alcohols, esters of higher fatty acids, waxes, polyglycols);

- special additives (preservatives, antioxidants, perfumes, etc.);

- active substances as such and in combination with excipients.

The properties of the product are determined by a combination of various components and their quantitative ratio.

Known tool "Septustin-Antisept" (manufacturer LLC "Uralstinol Bio", Russia), intended for hygienic processing of the hands of medical personnel, workers of perfumery and cosmetic enterprises, catering, the population in everyday life. The tool "Septutin-Antisept" has antimicrobial activity against gram-negative and gram-positive bacteria (including mycobacterium tuberculosis) and is a ready-to-use solution in the form of a yellowish liquid. As active substances, the product contains alkyldimethylbenzylammonium chloride (0.10%), N, N-bis (3-aminopropyl) dodecylamine (0.10%), isopropyl alcohol (70%), the rest is water.

The disadvantages of the described product include its toxicity (danger from contact with wounded skin and mucous membranes), as well as fire hazard.

Closest to the claimed is the antiseptic cream "Bepanten Plus" (manufacturer Waueg Cons. Care AG, Germany), intended for skin disinfection. “Bepanten Plus” has a pronounced bactericidal effect, is rapidly absorbed into the skin. This cream is used as a prophylactic and contains: chlorhexidine dihydrochloride 0.5%, dexpantheol 5.0%, a mixture of polyethylene oxides as a base up to 100%. The chlorhexidine dihydrochloride included in the formulation has a pronounced antimicrobial activity against many gram-negative and some gram-positive bacteria, including mycobacterium tuberculosis. However, when using this product, allergic skin reactions are possible in case of hypersensitivity to the components of the drug.

The objective of the invention is to increase the bactericidal effect of the drug against mycobacterium tuberculosis and reduce side effects.

The problem is solved by the inclusion in the inventive product for the skin, in addition to the well-known anti-TB drugs, stabilized sol of silver nanoparticles. The essence of the invention lies in the potentiating synergistic effect of the stabilized sol of silver nanoparticles, which enhances the bactericidal properties of the drug.

The inventive skin prophylactic with anti-tuberculosis effect includes streptomycin, a potentiating component — a stabilized sol of silver nanoparticles and a mixture of polyethylene oxides (PEO) grades 400 and 1500 as a basis in the following ratio of components, wt.%:

PEO-1500 5.0-15.0 Stabilized sol 7.0-10.0 silver nanoparticles Streptomycin 0.50-0.75 PEO-400 up to 100

Despite the fact that the components of the claimed funds are part of various pharmacological compositions, however, the claimed tool itself, having the indicated combination of components in a certain quantitative ratio, was not previously known.

The inventive skin prophylactic with anti-tuberculosis effect was obtained as follows.

Example 1. Preparation of funds (based on 100 wt.%)

Under aseptic conditions, when heated to 40-55 ° C, 5 wt.% PEO-1500, 7 wt.% Stabilized silver nanoparticle sol, 0.50 wt.% Streptomycin are mixed, cooled to room temperature, brought to 100 wt.% PEO- 400 and mixed with simultaneous homogenization. The test results of the drug are shown in the table (example 1).

Example 2

While maintaining the conditions of example 1 take 10 wt.% PEO-1500, 8 wt.% Stabilized sol of silver nanoparticles, 0.60 wt.% Streptomycin.

Example 3

While maintaining the conditions of example 1 take 15 wt.% PEO-1500, 9 wt.% Stabilized sol of silver nanoparticles, 0.75 wt.% Streptomycin.

Example 4

While maintaining the conditions of example 1 take 10 wt.% PEO-1500, 10 wt.% Stabilized sol of silver nanoparticles, 0.65 wt.% Streptomycin.

Example 5

While maintaining the conditions of example 1 take 10 wt.% PEO-1500, 7 wt.% Stabilized sol of silver nanoparticles, 0.50 wt.% Streptomycin.

The study of the suppression of the growth of Mycobacterium tuberculosis (MBT) was carried out using a dense Livenstein-Jensen nutrient medium. Material for research was obtained in the bacteriological laboratory of the State Health Institution of the Tuberculosis Hospital, from 30 patients with various forms of skin tuberculosis. The evaluation of the results of the study was carried out by counting the colonies in the experimental samples (examples 1-5) in comparison with the control (example 6 - prototype "Bepanten Plus"). Three degrees of inhibition of the growth of colonies of the Office (complete, significant, moderate) and the absence of inhibition of growth were noted.

Summary results on the study of the ability of the proposed drug and the prototype drug to inhibit the growth of strains of the office are presented in the table. In all cases, revealed a large bactericidal activity of the claimed funds in comparison with the prototype.

In addition, an unexpected effect was obtained when evaluating the possibility of using an agent for the treatment of psoriasis. So, the composition was tested on 5 patients with various forms of psoriasis (plaque psoriasis, pustular psoriasis, psoriatic erythroderma). The inventive tool was applied to the affected areas 3 times a day daily. An improvement in the general condition was observed after 8-10 weeks of treatment and was manifested in a significant reduction in the area of the affected area. The skin on the body is dry, clean, without color and signs of damage.

Table The results of the study of the overwhelming ability of drugs on the growth of strains of mycobacterium tuberculosis The degree of inhibition of the growth of the office,% Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 prototype Full 29th 42 52 61 fifty 12 Significant 40 35 thirty 31 41 21 Moderate 21 16 fourteen 8 6 5 Lack of 10 7 four - 2 62 Total one hundred one hundred one hundred one hundred one hundred one hundred

Claims (1)

Preventive skin product with anti-tuberculosis effect, containing polyethylene oxides (PEO) grades 400 and 1500 as a base and active substances, characterized in that it contains streptomycin and a stabilized sol of silver nanoparticles in the following ratio of components, wt.%:
PEO-1500 5.0-15.0 Stabilized Sol of Silver Nanoparticles 7.0-10.0 Streptomycin 0.50-0.75 PEO-400 up to 100