JPH0215012B2 - - Google Patents

Description

[Detailed description of the invention]

[Industrial Application Field] The present invention relates to an automatic concentration analyzer for measuring the concentration of various substances such as components in body fluids, and more specifically, to an automatic concentration analyzer for measuring the concentration of various substances such as components in body fluids.
The present invention relates to an automatic concentration analyzer that measures the concentrations of various substances by reading patterns generated by each reactant as optical densities. [Conventional technology] Conventionally, antigens obtained by immunoelectrophoresis, etc.
Image reading methods and devices are known that measure the concentration of various components contained in a sample based on the concentration of sedimentation lines generated by antibody reactions or the optical density of patterns obtained by various reactions. . [Problems to be Solved by the Invention] However, when a commercially available image pickup tube or a semiconductor image sensor is used as an image reading device, there is a drawback that there are many variations in the photoelectric conversion elements that constitute each pixel. It was hot. Particularly when the output of each element is related to the concentration of various components, differences in dark current, sensitivity curves, and saturation curves between each element appear as output differences, which can cause errors in measurement results. I had the disadvantage of giving. Therefore, the assembly of photoelectric conversion elements used in the optical reading device used in automatic analyzers must be carefully selected so that there is no variation among the elements, which naturally results in a high unit price. It had the disadvantage of being stored away. The present invention has been made in order to eliminate the above-mentioned drawbacks, and it is possible to determine the characteristics of each image sensor of an image sensor for optical reading, reduce reading errors during measurement, and to reduce the reading error during measurement. It is an object of the present invention to provide an automatic concentration analyzer having a highly accurate image density reading function even when using the present invention. [Means for Solving the Problems] In order to achieve the above object, the automatic concentration analyzer of the present invention, as shown in FIG. An XY drive unit 1 that controls the XY drive unit and an XY drive unit 2 that controls this XY drive unit.
, an image reading device 4 disposed above the XY drive base through a lens 3, an AD conversion circuit 5 that converts the output signal of this image reading device into a digital signal, and an AD conversion circuit connected to this AD conversion circuit. A first memory 6 that stores signals from the circuit; an arithmetic device 7 connected to this first memory and the XY drive device 2; and a first memory 6 that is connected to this arithmetic device and stores parameters related to correction for arithmetic processing. 2 memory 8, a third memory 9 for storing corrected data after arithmetic processing, and a recording device 10. Find the quadratic curve of the correction amount y, store each coefficient of the quadratic curve corresponding to each pixel, and calculate the output obtained by each pixel by
This is characterized in that correction calculations are performed using the following curves. [Operation] In order to match the sensitivity curve of each pixel to the average sensitivity curve of all pixels, find a quadratic curve of light intensity x versus correction amount y, store each coefficient of the quadratic curve corresponding to each pixel, The output obtained by each pixel is corrected using the quadratic curve (details will be explained in the embodiment). [Example] Hereinafter, an example of the present invention will be described based on the drawings. FIG. 1 shows an example of the configuration of an automatic concentration analyzer according to the present invention. The apparatus of the present invention includes an XY drive platform 1 that carries an object to be measured and moves in the
and an XY drive device 2 that controls this XY drive platform 1.
, an image reading device 4 disposed above the XY drive base 1 through a lens 3, an AD conversion circuit 5 that converts the output signal of the image reading device 4 into a digital signal, and an AD conversion circuit 5 that converts the output signal of the image reading device 4 into a digital signal. a first memory 6 for storing signals; an arithmetic device 7 connected to the first memory 6 and the XY drive device 2;
A second memory 8 and a third memory connected to this arithmetic device 7
Consisting of a memory 9 and a recording device 10, in order to match the sensitivity curve of each pixel to the average sensitivity curve of all pixels, a quadratic curve of light intensity x versus correction amount y is determined, and a quadratic curve corresponding to each pixel is calculated. It is configured to store each coefficient of , and perform a correction calculation on the output obtained by each pixel using the quadratic curve. Raw data from the image reading device 4 is written into the first memory 6, while corrected data after arithmetic processing is written into the third memory 9.
Parameters related to correction for arithmetic processing are written in the second memory 8. Prior to measurement, the following processing is performed to obtain parameters related to correction. First, when the image reading device 4 is composed of N elements, a uniform reflector such as ground glass is placed on the XY drive platform 1, and the amount of light is changed in stages.
At each stage, respective outputs for N elements are obtained. These are sequentially stored in one memory 6. Note that the light amount is changed in n steps. Next, in order to improve reliability and eliminate errors caused by non-uniformity such as frosted glass, dirt, and scratches, the XY drive platform 1 is moved a little, and the above-mentioned light intensity is changed in stages, and the light intensity for each of the N elements is changed. A process of obtaining the output is performed, and the output is added to the address in the first memory 6 corresponding to the amount of light at each stage and each element.
The above movement of the XY drive base 1 is performed a predetermined number of times (assumed to be m times), and the total output over m times is stored at a predetermined address in the first memory 6, Isum (i, j).
(However, i=1, 2,...N, j=1, 2,...n)
From, the output I(i, j) per time is expressed as the formula I(i,
j) = Isum (i, j)/m, and further N
the average value at the j-th time point for the elements
Mean(j) is determined from the formula Mean(j)= _N 〓 ⁱ⁼¹ I(i, j)/N (where j=1, 2,...n). Here, y _i (j) and x _i (j) are defined as y _i (j)=I(i, j)−Mean(j) x _i (j)=I(i, j), and for example, For the i-th element, the relationship between x _i (j) and y _i (j) is graphed to obtain a curve, and an equation corresponding to this curve is obtained. In general, a sufficient result can be obtained by approximating it as a quadratic equation, so y _i (j)=A _1,i +A _2,i x _i (j) +A _3,i {x _i (j)} ² ( i=1, 2,...N), and the coefficients of the quadratic equation at the i-th are A _1,i , A _2,i ,
A _3,i is required. When focusing only on a specific i-th element, the above equation represents x _i (j) and y _i (j) as continuous variables x and y, respectively, and simply becomes y = A ₁ +
It can be rewritten as A _2x + A _3x ² . i from 1 to N, and this coefficient value is stored in the second memory 8. Figure 2 is a graph showing the characteristics of the 88th element (i = 88), and Figure 3 is a graph showing the characteristics of the 88th element (i = 88).
Figure 4 is a graph showing the characteristics of the 90th (i=90) element. In other words, the horizontal axis of the graphs in Figures 2 to 4 shows the light intensity x, and the vertical axis shows the deviation y from the average value of the whole element, and as the light intensity is changed, the light intensity of the whole element changes. It can be understood from the three graphs that each element has a difference in sensitivity with respect to the average curvature. As described above, the values of A ₁ , A ₂ , and A ₃ for the 1st to Nth elements are stored in the second memory 8 as shown in the following table, and the processing for parameters related to correction is completed.

〔Effect of the invention〕

As explained above, the automatic concentration analyzer of the present invention not only simply equalizes the sensitivity variations of each element, but also averages the sensitivity curve itself, that is, the sensitivity curve of each pixel is By matching the average sensitivity curve, measurement accuracy is increased, and the correction amount also corrects deviations from the average value.
Since the value can be approximated to the average value, it has various excellent effects such as not requiring such a large correction and requiring only a small amount of correction. Note that the above correction can be applied to both a one-dimensional image sensor in which photoelectric elements are arranged in a row, and an image sensor that captures images in a two-dimensional plane.

[Brief explanation of drawings]

FIG. 1 is a systematic explanatory diagram showing one embodiment of the automatic concentration analyzer of the present invention, FIGS. 2 to 4 are graphs showing an example of characteristics of elements constituting the image reading device, and FIG. FIG. 6 is a diagram showing an example of printing out the raw output before correction, and FIG. 6 is a diagram showing an example of printing out the output after correction. 1...XY drive stand, 2...XY drive device, 3...
... Lens, 4 ... Image reading device, 5 ... AD conversion circuit, 6 ... First memory, 7 ... Arithmetic device, 8
...Second memory, 9...Third memory, 10...Storage device.

Claims (1)

[Claims] 1 XY drive stand 1 that carries the object to be measured and moves in the X- and Y-axis directions, and controls this XY drive stand
XY drive device 2 and lens 3 above the XY drive base
an image reading device 4 disposed through the image reading device, an AD conversion circuit 5 that converts the output signal of the image reading device into a digital signal, and a first memory connected to the AD conversion circuit and storing the signal from the AD conversion circuit. 6, an arithmetic device 7 connected to the first memory and the XY drive device 2, a second memory 8 connected to this arithmetic device and for storing parameters related to correction for arithmetic processing, In order to match the sensitivity curve of each pixel to the average sensitivity curve of all pixels, a quadratic curve of light intensity x versus correction amount y is determined. 2 corresponding to each pixel
An automatic concentration analyzer characterized in that each coefficient of a quadratic curve is stored, and an output obtained by each pixel is corrected using the quadratic curve.