JPS58225344A - Automatic analytical apparatus - Google Patents

Abstract

PURPOSE:To carry out the reading of image intensity in high measuring preciseness, by aligning the snesitivity curve of each image element obtained from a photoelectric conversion element to the average sensitivity curve of all image elements. CONSTITUTION:An object to be measured is placed on an XY drive stand 1 and the output of an image reading apparatus 4 is temporarily stored by first memory 6 through an AD conversion circuit 5. The relation of light intensity and output with respect to each photoelectric conversion element of an image reading apparatus 4 constituting each image element is stored by the predetermind address within said first memory 6. Succeedingly, the information in the memory 6 corresponding to each image element is successively read out and the curve of secondary degree of compensation output with respect to light intensity is calculated in order to align the sensitivity curve of each image element to the average sensitivity of all image elements. At the same time, coefficient is read out from secondary memory 8 storing each coefficient of the curve of secondary degree corresponding to each image element and subjected to compensating operation according to the aforementioned curve of secondary degree by an operation apparatus 7 to obtain the output.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides an automatic analyzer for measuring the concentration of various substances such as body fluid components. This is a book about automatic analyzers that measure the concentration of various substances by reacting various substances with substances that specifically react with them, reading the pattern produced by each reacting substance as optical density, and measuring the concentration of various substances. Therefore, in particular, the characteristics of each image sensor of the image sensor for optical reading are determined.

The purpose is to reduce reading errors during measurements.

Conventionally, M! 4 antigens.

An image reading device that measures the concentration of various components contained in a sample based on the concentration of a sedimentation line generated by an antibody reaction or the optical density of a pattern obtained by various reactions is a general commercially available image pickup tube or a semiconductor device. When using a so-called image sensor, there was a drawback that there were many variations in the photoelectric conversion elements constituting each pixel. When the output of each element is related to the concentration of various components, the difference in dark current between each element - the difference in sensitivity curve - the difference in saturation curve appears as a difference in output, resulting in an error in the measurement result. It had the disadvantage of giving , l.Therefore, an assembly of photoelectric conversion elements used in an optical reading device used in an automatic analyzer requires carefully selected materials that do not cause variations between each element17. There was a drawback that the middle part of each piece was high and Lt was high.

The present invention has been made to solve the drawbacks mentioned in 1. It is an object of the present invention to provide an automatic analyzer equipped with a highly accurate image density reading function when a commercially available general image sensor is used. It's a book.

Hereinafter, the configuration of the present invention will be explained based on the drawings.

FIG. 1 shows an example of the configuration of the σ) apparatus of the present invention. The apparatus of the present invention includes an XY drive stand 1 that carries an object to be measured and moves in the
A lens 3 is connected to one of the drive device 2 and the -xy drive stand 1.
An image reading device 4 disposed in the second image reading device 4 and an AD conversion circuit 5 for converting the output signal of the image reading device 4 into a digital signal.
It consists of a memory 6, an arithmetic device 7 connected to the first memory 6 and the xy drive device 2, a second memory 8 connected to the arithmetic device 7, a third memory 9, and a recording device 10. In order to match the sensitivity curve of a pixel to the average sensitivity curve of all pixels, a quadratic curve of one light intensity versus complementary IF output is determined, and each coefficient of the quadratic curve corresponding to each pixel is stored.11. The output obtained by each pixel is corrected by the quadratic curve.

Raw data from one image reading device 4 is written into the first memory 6, while corrected data after arithmetic processing is written into the third memory 9.

In the second memory 8, parameters related to correction of arithmetic processing errors are written. Prior to measurement, the following processing is performed in order to obtain parameters related to correction.

First, when the image reading device 4 is composed of N elements, there is a ground glass etc. on the XY drive base 1.
□ Place a uniform reflector and change the amount of light in stages.

At each stage, respective outputs for N elements are obtained.

This is stored in 1 memory B in order. Note that the light amount is changed in n steps. Next, in order to improve reliability and eliminate errors caused by non-uniformities such as frosted glass, dirt, and scratches, the -xy drive platform 1 was moved a little, and the above-mentioned light intensity was changed stepwise to obtain the results for N elements. The process of obtaining each output is performed by adding the amount of light at each stage and the address output in the first memory 6 corresponding to each element.

In the following, the movement of the xy drive platform 1 shall be 1 m times as specified)
First, the sum of m outputs stored at a predetermined address in the first memory 6 is Isum (N, n) (just I,
N=1.2. -=N, n=1.2. −=n ) and the mean value at time n for N elements
Find (n) (1. n = i, 2...n).

yN = Isum(N, n)−Mean(n)x
Since N=Isum(N, n) - for example, for the Nth element - graph 17 a curve and find the equation corresponding to this curve. In general, a sufficient result can be obtained by approximating the quadratic formula [, - yN=A,,N-4-A,,N'xN+A,l,Nx"
N (N = 1.2.', , N) and the coefficient A1. of the quadratic equation at the 17-Nth. N-At, N'-AIl, and N are obtained. N from 1 to N - set this coefficient value to the second
Store it in memory 8.

FIG. 2 is a graph showing the characteristics of the 88th element, FIG. 3 is a graph showing the characteristics of the 89th element, and FIG. 4 is a graph showing the characteristics of the 90th element. That is, the horizontal axis of the graphs in FIGS. 2 to 4 represents the light intensity. The vertical axis shows the deviation of the element from the average value of the whole element, and as the luminous intensity is changed, the average sensitivity of the whole element changes by 1. It can be understood from the three graphs that each of the seven elements has a difference in sensitivity. In addition, Figures 2 to 4
The y-axis of the graph shown in the figure is not the total output over m times, but the output for one time is displayed. As described above, each A1, A, Asσ for the −1 to Nth elements
'Iil[ is stored in the second memory 8 as shown in the following table,
The processing of parameters related to correction is completed.

In order to perform actual concentration measurement using the apparatuses 1 to 5, the object to be measured is placed on the 6, and then sequentially read out the information in the first memory 6 corresponding to each pixel, and at the same time read out the information in the second memory 8 σ)A, ~A
Read the coefficient of R i-1゜y-AItoA2x 4-A
, x' - This y becomes the corrected value.

Figure 5 is an example of printing out the raw output of supplementary iF and rail, and shows the object to be measured and 1. Using frosted glass, the vertical axis represents the light intensity and 1. This is an example. This is corrected by the wave q
, and K, a compensated output as shown in FIG. 6 is obtained. Even if the light intensity is varied, this effect changes and a good corrected output can be obtained.

As explained above, the automatic analyzer of the present invention not only equalizes the sensitivity variations of each element, but also equalizes the sensitivity curve itself. Measurement accuracy increases by matching the pixel's IV average sensitivity curve.

The correction amount also corrects the deviation from the average value from 1 to 517. The more σ) it is possible to get close to the average value, the larger the complement 1F
It has various excellent effects, such as not requiring any replenishment and requiring only a small amount of replenishment. Note that the above correction can be applied to both a one-dimensional (σ) image sensor in which photoelements T are arranged in rows and an image sensor that captures images in two-dimensional terms.

[Brief explanation of the drawing]

Figure 1 is a systematic explanatory diagram showing one embodiment of the dynamic analysis device of the present invention - Figures 2 to 4 are graphs showing examples of characteristics of elements constituting the image reading device - FIG. 5 is a diagram showing an example of printing out the output of a cow before correction, and FIG. 6 is a diagram showing an example of printing out the output after correction. 1...XY drive stand, 2...XY drive device-3...Lens-4...Image reading device-5...AD conversion circuit, 6
...First memory 1L7...Arithmetic unit -8...-Second memory 9...Third memo +7. IQ...Recording device patent applicant Makoto Shiide-1, patent attorney representing Toa Medical Electronics Co., Ltd. (pa)

Claims (1)

[Scope of Claims] 1. An xy drive base that carries an object to be measured z1 and moves in the X-axis and Y-axis directions, an xy drive device that controls the xy drive base, and a lens above the -xy drive base. Intervention 1. - an AD conversion circuit that converts the output signal of the image reading device into a digital signal; - a first memory that stores the signal from the AD conversion circuit; - the first memory and the XY drive; The apparatus includes a computing device connected to the computing device, a second memory connected to the computing device, a third memory, and a recording device. Find the quadratic curve of the corrected output. An automatic analyzer characterized in that each coefficient of a quadratic curve corresponding to each pixel is stored L, and an output obtained by each pixel is corrected using the quadratic curve.