JPH02138127A - Growth inhibitor for intestinal noxious bacterium - Google Patents

Abstract

PURPOSE:To obtain a growth inhibitor for intestinal noxious bacteria, containing flesh of a bottle gourd as an active ingredient, capable of exhibiting remarkable growth promoting effects on bacteria of the genus Bifidobacterium and further growth inhibitory effects on enterococci, Psuedomonas aeruginosa, etc., and expected as an immunopotentiator, etc. CONSTITUTION:A growth inhibitor containing flesh of a bottle gourd which is a cucurbitaceous annual vine as an active ingredient. The above-mentioned inhibitor can be produced by steam sterilization under 0.2-0.4kg/cm<2> steam pressure for 5-20min sterilization time or alcoholic sterilization with an alcohol in an amount of 15-150g/kg (dried bottle gourd) for a sterilization period of 1-3 days. The afore-mentioned inhibitor has <=5X10<3> cells/g normal viable cell number, negative to the coliform group and Salmonella with <=8% moisture content and is extremely safe in food sanitation. For example, additives, such as sweet corn or lactose, can be used as the afore-mentioned inhibitor.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to an agent for inhibiting the growth of harmful intestinal bacteria, which contains the pulp of Yugao as an active ingredient.

Conventional technology Yugao is an annual vine belonging to the Cucurbitaceae family, and its fruit is oval in shape, about 70 centimeters in diameter. Ganhong is made by peeling the pulp of this fruit into long, string-like pieces and drying them.

Until now, it has not been known that the pulp of Yugao suppresses the growth of harmful bacteria in the intestines.

Problems to be Solved by the Invention Known bifidus factors are used by other intestinal bacteria in vivo, so they do not have a sufficient growth effect on Bifidobacterium bacteria, and their production methods are complicated. It has drawbacks such as being expensive.

Therefore, a bifidus factor that has a sufficient effect of promoting the growth of bifidobacteria and suppressing the growth of harmful intestinal bacteria, and can be produced easily at low cost, would be extremely useful industrially.

Means for Solving the Problems Some of the present inventors have already conducted intensive research on the bifidus factor, and have found that Yugao food processed from Yugao pulp selectively and significantly promotes the growth of Bifidobacterium bacteria. (Japanese Patent Application No. 123761/1982). We have now completed the present invention by discovering that the pulp of Yugao has the effect of inhibiting the growth of harmful bacteria in the intestines.

The intestinal harmful bacteria growth inhibitor of the present invention is obtained by processing the pulp of Yugao into a desired shape such as powder, granules, tablets, etc., and may or may not be sterilized.

The sterilized intestinal harmful bacteria growth inhibitor of the present invention is steam sterilized with a steam pressure of 0.2 to 0.4 kmcm2 and a sterilization time of 5 to 20 minutes, or an alcohol addition amount of 15 to 150 q/kg (Yugao dried product). It can be produced by alcohol sterilization for 1 to 30 days. This sterilized intestinal harmful bacteria growth inhibitor has a general viable bacterial count of 5 x 103 cells/q or less, is negative for coliform bacteria and salmonella, and has a water content of 8% or less, making it extremely safe in terms of food hygiene.

Additives may be added to the intestinal harmful bacterial growth inhibitor of the present invention, such as sweet corn, carrot powder, corn fiber apple fiber,
Pumpkin powder, fiber components such as alginic acid, excipients such as lactose and starch, sweetening components such as white sugar, maltose, sorbitol, and mannitol, milk powder, meat extract,
Nutrient supplements such as vitamins, lubricants such as gum arabic powder, polyvinyl pyrrolidone, hydroxypropyl cellulose, sodium carboxymethyl cellulose, magnesium stearate, calcium stearate, love wax, and tarari are included, and anti-proliferative agents. They may be appropriately selected and used depending on the shape and/or preference.

Among the intestinal harmful bacterial growth inhibitors of the present invention, those in powder or granule form may be added to soup bases, furikake, or to sweets, noodles, tofu, and other foods. In addition, it can be used as a feed additive for livestock such as cows and pigs, and as a feed additive for pets such as dogs and cats.

Effect The inhibitory effect of the Yugao pulp powder of the present invention on the growth of harmful intestinal bacteria will be explained.

Test 1 As samples, a powdered Yugao pulp produced in the same manner as in Example 1 and a sterilized powdered Yugao pulp produced in the same manner as in Example 5 were used.

Each sample was added to Tomarelli medium (Journal of Biochemical Chemistry, Vol. 181, p. 879, 1949), and Bifidobacterium coum and other intestinal bacteria were cultured at 37°C for 48 hours. After culturing, the culture solution was applied to a BL agar medium and cultured at 31° C. for 48 hours, and then the number of bacteria was measured.

The results are shown in Table 1.

(Left below) As is clear from Table 1, Yugao pulp powder and sterilized Yugao pulp powder have a remarkable growth-promoting effect on Bifidobacterium, while enterococcus, Pseudomonas aeruginosa, Escherichia coli, Clostridium perfringens, etc. It is not utilized by harmful intestinal bacteria; on the contrary, it exhibits a growth-inhibiting effect on these bacteria.

Example 1 Peel the outer skin of Yugao thinly, slice the pulp into thin strips,
After drying in the sun, it is dried at 70'C for 5 hours to obtain a strip-shaped Yugao pulp with a moisture content of about 3%. 30 kQ of this dried product was pulverized with a pulverizer (manufactured by Hoiyo Tekkosho) to obtain 24 kq of Yugao pulp powder.

Example 2 950 q of the powder obtained in Example 1 and 40 g of hydroxypropyl cellulose were mixed in a mixer for 10 minutes, then purified water was added, kneaded, and dried with hot air. After cooling, this was sized using an oscillator (manufactured by Kikusui Seisakusho) to obtain 960 q of Yugao pulp granules.

Example 3 After mixing 495 g of the granules obtained in Example 2 and 5 g of magnesium stearate in a mixer for 10 minutes, the mixture was compressed to obtain 4,400 Yugao pulp tablets each weighing 110 mu.

Example 4 30k of band-shaped Yugao fruit pulp obtained in the same manner as Example 1
．． After sterilization for 20 minutes under a steam pressure of 2kMCII12,
It was dried with hot air at 60° C. until the moisture content was about 3%.

Next, this was operated in the same manner as in Example 1 to obtain 25k (J)q of sterilized evening glory pulp powder.

This powder has a general viable bacterial count of 2. Ox 102/q, negative for E. coli and Salmonella.

Example 5 30 kg of strip-shaped Yugao pulp obtained in the same manner as in Example 1 was sprayed with 2.7 kg of ethanol (90 g/kg of strip-shaped Yugao pulp), sealed and left at room temperature for 5 days, and then operated in the same manner as in Example 1. 25.7 kg of sterilized Yugao pulp powder was obtained.

This powder had a general viable bacterial count of 37/g and was negative for E. coli and Salmonella.

Effects of the Invention The agent for inhibiting the growth of harmful intestinal bacteria of the present invention selectively and significantly promotes the growth of Bifidobacterium bacteria and inhibits the growth of harmful intestinal bacteria. ■It is also expected to be used as a stimulant and anticancer drug.

Patent applicant: Tokyo Tanabe Pharmaceutical Co., Ltd. Representative Patent Attorney Naoyuki Matsuyama

Claims (1)

[Claims] (1) An inhibitor of the growth of harmful intestinal bacteria whose active ingredient is the pulp of Yugao.