JPH01289479A - Rotational culturing vessel for plant - Google Patents

Abstract

PURPOSE:To readily and adequately culture cell of plant by enclosing a culturing solution in a plant culturing vessel constructed in a shape of circular rotating vessel and introducing light and gas into said culturing solution through an optical fiber and gas feeding tube. CONSTITUTION:A culturing solution 13 containing cells of plant such as flesh of tomato and herb, etc., and hormone is enclosed in a cylindrical culturing vessel 6 placed on at least two rotating rollers 5 so as to horizontally keep rotational axes. Next, light and gas of suitable component are introduced into said culturing solution 13 through a gas feeding tube 10 penetrated and supported by a circular cap part 7 and an optical fiber 11, then gas arisen in the culturing solution 13 is exhausted to outside through an exhaust tube 12, thus a culturing of cell of plant is readily carried out.

Description

[Detailed description of the invention] Industrial applications The present invention relates to a plant cell culture vessel.

Conventionally, plant cell culture vessels for culturing root cells such as shikonin have been known, but these have been limited to those that do not require light.

The present invention aims to construct a plant incubator as a rotating container, and introduce air and an appropriate amount of light into the container to cultivate the plant cells themselves.

1-One aspect The present invention comprises: a circular cap portion that penetrates and supports an air supply pipe, an exhaust pipe, and an optical fiber; a cylindrical container whose mouth is coupled to the cap portion by a rotary seal mechanism; It consists of at least two rotating rollers on which the cylindrical container is placed so that its center axis is substantially horizontal, and an air pipe and an optical fiber are introduced into the container. Air is not limited to what is called air, but also includes gases in which oxygen, carbon dioxide, etc. are appropriately adjusted.

FIG. 1 is a side view for explaining one embodiment of the present invention;
FIG. 2 is a front view, and in the figure, reference numeral 1 denotes a base that houses a control device. A drive device 2 provided with a motor 3 is attached to the base 1. The motor 3 drives the rotating shaft 4 via a suitable speed reduction mechanism.

Reference numeral 5.5 denotes two rotary shafts for rotatably supporting the culture container 6, which are supported by bearings on the base 1 and connected to the rotary shaft 4 so that the rotary shafts are horizontal. The culture container 6 is a cylindrical container arranged so that its central axis is horizontal. Reference numeral 7 denotes a cap portion of the culture container 6, 8 a band for tightening the cap portion 7, and 9 a support member for the cap portion 7, which is engaged with a support 9a attached to the base 1. 10 is an air pipe, 11 is an optical fiber, and 12 is an exhaust pipe.

FIG. 3 is a cross-sectional view for explaining the details of one base portion 7, in which the lid body 15 includes an air pipe 1o, an optical fiber 11. Supports the exhaust pipe 12. 20 is screwed onto the cap body 16 to form the lid body 1
5 to the base body 16. 21
The base body 16, which is a silicone backing that seals the introduction hole of the lid body 15, and the culture vessel are configured to be rotatable in a sealed state by a bearing 17 and an oil seal 18, and are connected by a fixing ring 19.

Since the structure is as described above, the culture container 6 is rotated in the direction of the arrow 14 while being held on the roller 5 by the driving force from the electric motor. A culture solution 13 containing plant cells and hormones is sealed in the culture container. Appropriate gases and light are guided into the culture solution 13 by an air pipe and an optical fiber. The light from the optical fiber is
Of course, artificial light in which visible light components from sunlight are introduced constantly or at appropriate time intervals may also be used. The amount of culture solution sealed in the culture container 6 is preferably half or less of the container internal volume.The amount of air introduced into the culture solution through the air pipe may be small, and the culture solution can be expanded by rotating the culture container. Supplied in sufficient quantity. The gas floating in the culture solution is exhausted to the outside through an exhaust pipe.

Various plant cells such as tomato pulp and medicinal herb cells can be used as the plant cells to be cultured. In the case of tomato pulp, tomato juice can be produced in a culture container by providing appropriate conditions.

As mentioned above, the present invention allows light to be introduced through optical fibers when culturing plant cells using a culture solution, so that the plant cells themselves can be cultured and the quality and quantity of the introduced light can be controlled. , irradiation time, etc. can be adjusted to suit culture, and it has become possible to culture plant cells, which was previously impossible.

In addition, since the culture container was placed on two rollers and rotated so that the rotation axis was horizontal, the depth of the culture solution was shallow, and there was less pressure on the bottom of the solution, which was good. can be cultured.

[Brief explanation of the drawing]

FIG. 1 is a side view of one embodiment of the present invention, FIG. 2 is a front view thereof, and FIG. 3 is a partial sectional view of the main part. 5...
Rotating roller, 6... Culture container, 7... Cap part, 1
0... Air pipe, 11... Optical fiber, 12...
Exhaust pipe, 13...Culture solution, 17...Bearing, 1
8...Oil seal. Figure 1 Figure 2

Claims (1)

[Claims] 1. A circular cap portion that penetrates and supports an air supply pipe, an exhaust pipe, and an optical fiber, and a cylindrical container whose mouth portion is connected to the cap portion by a rotary sealing mechanism and is coaxial with the cap portion;
A rotating plant culture device comprising at least two rotating rollers on which the cylindrical container is placed so that its center axis is substantially horizontal, and an air pipe and an optical fiber are introduced into the container.