JPH0117111B2 - - Google Patents
Info
- Publication number
- JPH0117111B2 JPH0117111B2 JP55129941A JP12994180A JPH0117111B2 JP H0117111 B2 JPH0117111 B2 JP H0117111B2 JP 55129941 A JP55129941 A JP 55129941A JP 12994180 A JP12994180 A JP 12994180A JP H0117111 B2 JPH0117111 B2 JP H0117111B2
- Authority
- JP
- Japan
- Prior art keywords
- blood
- solution
- density
- concentration
- separation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000926 separation method Methods 0.000 claims description 43
- 210000004369 blood Anatomy 0.000 claims description 29
- 239000008280 blood Substances 0.000 claims description 29
- 210000003743 erythrocyte Anatomy 0.000 claims description 23
- 239000000243 solution Substances 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 20
- 229920001917 Ficoll Polymers 0.000 claims description 19
- 239000007864 aqueous solution Substances 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 13
- 210000000224 granular leucocyte Anatomy 0.000 claims description 11
- 210000005087 mononuclear cell Anatomy 0.000 claims description 10
- 229920002307 Dextran Polymers 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- GGGDNPWHMNJRFN-UHFFFAOYSA-N metrizoic acid Chemical compound CC(=O)N(C)C1=C(I)C(NC(C)=O)=C(I)C(C(O)=O)=C1I GGGDNPWHMNJRFN-UHFFFAOYSA-N 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- MIKKOBKEXMRYFQ-WZTVWXICSA-N meglumine amidotrizoate Chemical compound C[NH2+]C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.CC(=O)NC1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I MIKKOBKEXMRYFQ-WZTVWXICSA-N 0.000 claims description 5
- 229920000642 polymer Polymers 0.000 claims description 5
- 230000004931 aggregating effect Effects 0.000 claims description 4
- 229960004712 metrizoic acid Drugs 0.000 claims description 4
- 229950000550 sodium metrizoate Drugs 0.000 claims description 4
- TXKOGNLDVKUFSI-WZTVWXICSA-N 3-acetamido-5-[acetyl(methyl)amino]-2,4,6-triiodobenzoic acid;(2r,3r,4r,5s)-6-(methylamino)hexane-1,2,3,4,5-pentol Chemical compound CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.CC(=O)N(C)C1=C(I)C(NC(C)=O)=C(I)C(C(O)=O)=C1I TXKOGNLDVKUFSI-WZTVWXICSA-N 0.000 claims description 2
- 230000004523 agglutinating effect Effects 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- ZEYOIOAKZLALAP-UHFFFAOYSA-M sodium amidotrizoate Chemical compound [Na+].CC(=O)NC1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I ZEYOIOAKZLALAP-UHFFFAOYSA-M 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 4
- 229930006000 Sucrose Natural products 0.000 claims 4
- 229920001282 polysaccharide Polymers 0.000 claims 3
- 210000000265 leukocyte Anatomy 0.000 description 19
- 210000003622 mature neutrocyte Anatomy 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 210000000601 blood cell Anatomy 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- MBBZMMPHUWSWHV-BDVNFPICSA-N N-methylglucamine Chemical compound CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO MBBZMMPHUWSWHV-BDVNFPICSA-N 0.000 description 4
- YVPYQUNUQOZFHG-UHFFFAOYSA-N amidotrizoic acid Chemical compound CC(=O)NC1=C(I)C(NC(C)=O)=C(I)C(C(O)=O)=C1I YVPYQUNUQOZFHG-UHFFFAOYSA-N 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 229960005423 diatrizoate Drugs 0.000 description 3
- 230000003067 hemagglutinative effect Effects 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- XZNXVSDNACTASG-RZNNTOFGSA-M sodium;3,5-diacetamido-2,4,6-triiodobenzoate;3,5-diacetamido-2,4,6-triiodobenzoic acid;(2r,3r,4r,5s)-6-(methylamino)hexane-1,2,3,4,5-pentol Chemical group [Na+].CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.CC(=O)NC1=C(I)C(NC(C)=O)=C(I)C(C(O)=O)=C1I.CC(=O)NC1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I XZNXVSDNACTASG-RZNNTOFGSA-M 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- 229920006158 high molecular weight polymer Polymers 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 229960003194 meglumine Drugs 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 210000000440 neutrophil Anatomy 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- YWLXLRUDGLRYDR-ZHPRIASZSA-N 5beta,20-epoxy-1,7beta,10beta,13alpha-tetrahydroxy-9-oxotax-11-ene-2alpha,4alpha-diyl 4-acetate 2-benzoate Chemical compound O([C@H]1[C@H]2[C@@](C([C@H](O)C3=C(C)[C@@H](O)C[C@]1(O)C3(C)C)=O)(C)[C@@H](O)C[C@H]1OC[C@]12OC(=O)C)C(=O)C1=CC=CC=C1 YWLXLRUDGLRYDR-ZHPRIASZSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000002433 mononuclear leukocyte Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55129941A JPS5754860A (en) | 1980-09-17 | 1980-09-17 | Blood density separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55129941A JPS5754860A (en) | 1980-09-17 | 1980-09-17 | Blood density separation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5754860A JPS5754860A (en) | 1982-04-01 |
| JPH0117111B2 true JPH0117111B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1989-03-29 |
Family
ID=15022212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55129941A Granted JPS5754860A (en) | 1980-09-17 | 1980-09-17 | Blood density separation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5754860A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1291098C (en) * | 1984-12-04 | 1991-10-22 | Albert August Luderer | Lymphocyte collection tube |
| KR20220151158A (ko) * | 2020-03-11 | 2022-11-14 | 세키스이 메디칼 가부시키가이샤 | 백혈구 농축 분리 디바이스, 혈액 채취 용기 및 백혈구의 분리 방법 |
-
1980
- 1980-09-17 JP JP55129941A patent/JPS5754860A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5754860A (en) | 1982-04-01 |
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