JPH0113059B2 - - Google Patents
Info
- Publication number
- JPH0113059B2 JPH0113059B2 JP56077455A JP7745581A JPH0113059B2 JP H0113059 B2 JPH0113059 B2 JP H0113059B2 JP 56077455 A JP56077455 A JP 56077455A JP 7745581 A JP7745581 A JP 7745581A JP H0113059 B2 JPH0113059 B2 JP H0113059B2
- Authority
- JP
- Japan
- Prior art keywords
- column
- catecholamine
- catecholamines
- sample
- separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 150000003943 catecholamines Chemical class 0.000 claims description 41
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 21
- 238000000926 separation method Methods 0.000 claims description 19
- 239000000872 buffer Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000007853 buffer solution Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 238000010979 pH adjustment Methods 0.000 claims description 5
- 238000010828 elution Methods 0.000 claims description 4
- 238000001179 sorption measurement Methods 0.000 claims description 4
- 238000003795 desorption Methods 0.000 claims description 2
- 238000005868 electrolysis reaction Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 description 31
- 238000004458 analytical method Methods 0.000 description 9
- 230000035945 sensitivity Effects 0.000 description 9
- UCTWMZQNUQWSLP-UHFFFAOYSA-N adrenaline Chemical compound CNCC(O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-UHFFFAOYSA-N 0.000 description 8
- 238000001514 detection method Methods 0.000 description 6
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 6
- 238000004811 liquid chromatography Methods 0.000 description 6
- 239000011347 resin Substances 0.000 description 6
- 229920005989 resin Polymers 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- SFLSHLFXELFNJZ-QMMMGPOBSA-N (-)-norepinephrine Chemical compound NC[C@H](O)C1=CC=C(O)C(O)=C1 SFLSHLFXELFNJZ-QMMMGPOBSA-N 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 239000004809 Teflon Substances 0.000 description 4
- 229920006362 Teflon® Polymers 0.000 description 4
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 4
- UUAGAQFQZIEFAH-UHFFFAOYSA-N chlorotrifluoroethylene Chemical group FC(F)=C(F)Cl UUAGAQFQZIEFAH-UHFFFAOYSA-N 0.000 description 4
- 229960002748 norepinephrine Drugs 0.000 description 4
- SFLSHLFXELFNJZ-UHFFFAOYSA-N norepinephrine Natural products NCC(O)C1=CC=C(O)C(O)=C1 SFLSHLFXELFNJZ-UHFFFAOYSA-N 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 3
- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- GTKRFUAGOKINCA-UHFFFAOYSA-M chlorosilver;silver Chemical compound [Ag].[Ag]Cl GTKRFUAGOKINCA-UHFFFAOYSA-M 0.000 description 3
- 230000003750 conditioning effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 229960003638 dopamine Drugs 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- 239000006171 Britton–Robinson buffer Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000003411 electrode reaction Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 239000012494 Quartz wool Substances 0.000 description 1
- 239000012556 adjustment buffer Substances 0.000 description 1
- 230000001919 adrenal effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000000035 biogenic effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 229910021397 glassy carbon Inorganic materials 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- REFMEZARFCPESH-UHFFFAOYSA-M sodium;heptane-1-sulfonate Chemical compound [Na+].CCCCCCCS([O-])(=O)=O REFMEZARFCPESH-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000004454 trace mineral analysis Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/64—Electrical detectors
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56077455A JPS57191548A (en) | 1981-05-20 | 1981-05-20 | Analysing apparatus of catechol amine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56077455A JPS57191548A (en) | 1981-05-20 | 1981-05-20 | Analysing apparatus of catechol amine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS57191548A JPS57191548A (en) | 1982-11-25 |
| JPH0113059B2 true JPH0113059B2 (enrdf_load_stackoverflow) | 1989-03-03 |
Family
ID=13634481
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56077455A Granted JPS57191548A (en) | 1981-05-20 | 1981-05-20 | Analysing apparatus of catechol amine |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS57191548A (enrdf_load_stackoverflow) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5987366A (ja) * | 1982-11-11 | 1984-05-19 | Shimadzu Corp | カテコ−ルアミンの分析用前処理キツト |
| JPH0675066B2 (ja) * | 1984-05-02 | 1994-09-21 | 株式会社島津製作所 | カテコ−ルアミン類検出装置 |
| JP3985953B2 (ja) * | 2002-08-15 | 2007-10-03 | 独立行政法人産業技術総合研究所 | 化学物質の高感度電気化学検出方法、及び化学物質の高感度検出装置 |
-
1981
- 1981-05-20 JP JP56077455A patent/JPS57191548A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS57191548A (en) | 1982-11-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kissinger et al. | Electrochemical detection of selected organic components in the eluate from high-performance liquid-chromatography | |
| US3923460A (en) | Dual ion chromatograph using parallel columns for ionic analysis | |
| Van Staden et al. | Flow-injection analysis systems with different detection devices and other related techniques for the in vitro and in vivo determination of dopamine as neurotransmitter. A review | |
| Goto et al. | Micro high-performance liquid chromatographic system with micro precolumn and dual electrochemical detector for direct injection analysis of catecholamines in body fluids | |
| WO2013062084A1 (ja) | 分析装置および分析方法 | |
| Zapata et al. | Detection and quantification of neurotransmitters in dialysates | |
| JPH0113059B2 (enrdf_load_stackoverflow) | ||
| Boppana et al. | Simplified procedures for the determination of fenoldopam and its metabolites in human plasma by high-performance liquid chromatography with electrochemical detection: comparison of manual and robotic sample preparation methods | |
| Froehlich et al. | An HPLC-Fluorimetric analysis for L-dopa, noradrenalin and dopamine | |
| Dieker et al. | Study of a glassy carbon electrode in amperometric detection using dc, normal and differential pulse techniques | |
| Kuldvee et al. | Nonconventional samplers in capillary electrophoresis | |
| JPH029307B2 (enrdf_load_stackoverflow) | ||
| De Castro et al. | Integration of separation and detection in continuous-flow systems | |
| JPH03205559A (ja) | 生体試料のクロマトグラフイー分析法および液体クロマトグラフ装置 | |
| US20160061787A1 (en) | Automated analysis systems | |
| CN115791994A (zh) | 用于离子色谱的检查标准品再循环设置 | |
| EP0429082A2 (en) | Method for analysis of catecholamine | |
| He et al. | A Cartridge Structure Facilitates Clinical Mass Spectrometry Pretreatment and Can be Integrated Into a High‐Throughput Instrument | |
| JPS60140151A (ja) | 細管式等速電気泳動分析装置 | |
| JPH1010107A (ja) | 液体クロマトグラフィーによる試料の分析方法 | |
| Kissinger | Microdialysis and liquid chromatography | |
| CN102236004B (zh) | 一种液相色谱电位型检测器及其检测方法 | |
| JPS63212861A (ja) | バニリルマンデル酸、ホモバニリン酸およびクレアチニンの同時分析方法およびその装置 | |
| Horstkotte et al. | Coupling of flow techniques with capillary electrophoresis: review of operation principles, challenges, potentials, and applications | |
| de Castro et al. | New approaches to coupling flow-injection analysis and high-performance liquid chromatography |