JP7854386B2 - 標的ゲノム領域の捕捉と分析 - Google Patents

標的ゲノム領域の捕捉と分析

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Publication number
JP7854386B2
JP7854386B2 JP2022506557A JP2022506557A JP7854386B2 JP 7854386 B2 JP7854386 B2 JP 7854386B2 JP 2022506557 A JP2022506557 A JP 2022506557A JP 2022506557 A JP2022506557 A JP 2022506557A JP 7854386 B2 JP7854386 B2 JP 7854386B2
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Japan
Prior art keywords
target
target genomic
region
recognition site
stranded
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JP2022506557A
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English (en)
Japanese (ja)
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JP2022534625A (ja
JP2022534625A5 (enExample
Inventor
ゴミデ ネヴェス，レアンドロ
Original Assignee
エルジーシー ゲノミクス エルエルシー
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Publication of JP2022534625A5 publication Critical patent/JP2022534625A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022506557A 2019-05-13 2020-05-13 標的ゲノム領域の捕捉と分析 Active JP7854386B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962846988P 2019-05-13 2019-05-13
US62/846,988 2019-05-13
PCT/US2020/032615 WO2020232081A2 (en) 2019-05-13 2020-05-13 Capture and analysis of target genomic regions

Publications (3)

Publication Number Publication Date
JP2022534625A JP2022534625A (ja) 2022-08-02
JP2022534625A5 JP2022534625A5 (enExample) 2023-04-25
JP7854386B2 true JP7854386B2 (ja) 2026-05-01

Family

ID=73289300

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022506557A Active JP7854386B2 (ja) 2019-05-13 2020-05-13 標的ゲノム領域の捕捉と分析

Country Status (10)

Country Link
US (1) US20220220542A1 (enExample)
EP (1) EP3969581B1 (enExample)
JP (1) JP7854386B2 (enExample)
KR (1) KR20220039653A (enExample)
CN (1) CN114286861B (enExample)
AU (1) AU2020275301A1 (enExample)
BR (1) BR112022001539A2 (enExample)
CA (1) CA3145806A1 (enExample)
ES (1) ES3051342T3 (enExample)
WO (1) WO2020232081A2 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4034675A4 (en) * 2019-09-26 2023-10-04 Jumpcode Genomics, Inc. Method and system for targeted nucleic acid sequencing
WO2022232425A2 (en) * 2021-04-29 2022-11-03 Illumina, Inc. Amplification techniques for nucleic acid characterization

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008515448A (ja) 2004-10-11 2008-05-15 バリー カレバウト アーゲー 料理におけるカカオバターの使用
JP2011234723A (ja) 2005-06-15 2011-11-24 Callida Genomics Inc 遺伝子解析および化学解析用の単分子アレイ
JP2017516487A (ja) 2014-06-06 2017-06-22 コーネル・ユニバーシティーＣｏｒｎｅｌｌ Ｕｎｉｖｅｒｓｉｔｙ ヌクレアーゼ、リガーゼ、ポリメラーゼ、及び配列決定反応の組み合わせを用いた、核酸配列、発現、コピー、またはｄｎａのメチル化変化の識別及び計数方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
CA2615323A1 (en) * 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
US20090156412A1 (en) 2007-12-17 2009-06-18 Helicos Biosciences Corporation Surface-capture of target nucleic acids
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9365897B2 (en) * 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
US9873907B2 (en) * 2013-05-29 2018-01-23 Agilent Technologies, Inc. Method for fragmenting genomic DNA using CAS9
HRP20200529T1 (hr) * 2014-06-06 2020-09-04 Regeneron Pharmaceuticals, Inc. Postupci i pripravci za modificiranje ciljnog lokusa
WO2016109255A1 (en) * 2014-12-30 2016-07-07 University Of South Florida Methods and compositions for cloning into large vectors
GB2565461B (en) * 2016-03-31 2022-04-13 Harvard College Methods and compositions for the single tube preparation of sequencing libraries using Cas9
GB201616590D0 (en) * 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
KR20220038604A (ko) * 2019-05-30 2022-03-29 래피드 제노믹스 엘엘씨 표적 게놈 영역의 유연한 및 고처리량 시퀀싱

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008515448A (ja) 2004-10-11 2008-05-15 バリー カレバウト アーゲー 料理におけるカカオバターの使用
JP2011234723A (ja) 2005-06-15 2011-11-24 Callida Genomics Inc 遺伝子解析および化学解析用の単分子アレイ
JP2017516487A (ja) 2014-06-06 2017-06-22 コーネル・ユニバーシティーＣｏｒｎｅｌｌ Ｕｎｉｖｅｒｓｉｔｙ ヌクレアーゼ、リガーゼ、ポリメラーゼ、及び配列決定反応の組み合わせを用いた、核酸配列、発現、コピー、またはｄｎａのメチル化変化の識別及び計数方法

Also Published As

Publication number Publication date
BR112022001539A2 (pt) 2022-03-22
ES3051342T3 (en) 2025-12-26
CN114286861B (zh) 2024-12-06
EP3969581B1 (en) 2025-10-08
JP2022534625A (ja) 2022-08-02
WO2020232081A3 (en) 2020-12-24
AU2020275301A1 (en) 2022-01-20
EP3969581A2 (en) 2022-03-23
EP3969581A4 (en) 2023-06-14
WO2020232081A2 (en) 2020-11-19
US20220220542A1 (en) 2022-07-14
KR20220039653A (ko) 2022-03-29
CN114286861A (zh) 2022-04-05
CA3145806A1 (en) 2020-11-19

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