CA3145806A1 - Capture and analysis of target genomic regions - Google Patents

Capture and analysis of target genomic regions Download PDF

Info

Publication number
CA3145806A1
CA3145806A1 CA3145806A CA3145806A CA3145806A1 CA 3145806 A1 CA3145806 A1 CA 3145806A1 CA 3145806 A CA3145806 A CA 3145806A CA 3145806 A CA3145806 A CA 3145806A CA 3145806 A1 CA3145806 A1 CA 3145806A1
Authority
CA
Canada
Prior art keywords
target genomic
genomic region
target
endonucleases
genomic regions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3145806A
Other languages
English (en)
French (fr)
Inventor
Leandro Gomide Neves
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LGC Genomics LLC
Original Assignee
Rapid Genomics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rapid Genomics LLC filed Critical Rapid Genomics LLC
Publication of CA3145806A1 publication Critical patent/CA3145806A1/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3145806A 2019-05-13 2020-05-13 Capture and analysis of target genomic regions Pending CA3145806A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962846988P 2019-05-13 2019-05-13
US62/846,988 2019-05-13
PCT/US2020/032615 WO2020232081A2 (en) 2019-05-13 2020-05-13 Capture and analysis of target genomic regions

Publications (1)

Publication Number Publication Date
CA3145806A1 true CA3145806A1 (en) 2020-11-19

Family

ID=73289300

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3145806A Pending CA3145806A1 (en) 2019-05-13 2020-05-13 Capture and analysis of target genomic regions

Country Status (10)

Country Link
US (1) US20220220542A1 (enExample)
EP (1) EP3969581B1 (enExample)
JP (1) JP7854386B2 (enExample)
KR (1) KR20220039653A (enExample)
CN (1) CN114286861B (enExample)
AU (1) AU2020275301A1 (enExample)
BR (1) BR112022001539A2 (enExample)
CA (1) CA3145806A1 (enExample)
ES (1) ES3051342T3 (enExample)
WO (1) WO2020232081A2 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4034675A4 (en) * 2019-09-26 2023-10-04 Jumpcode Genomics, Inc. Method and system for targeted nucleic acid sequencing
WO2022232425A2 (en) * 2021-04-29 2022-11-03 Illumina, Inc. Amplification techniques for nucleic acid characterization

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2876249A1 (fr) 2004-10-11 2006-04-14 Barry Callebaut Ag Utilisation du beurre de cacao dans des preparations culinaires
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
CA2615323A1 (en) * 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
US20090156412A1 (en) 2007-12-17 2009-06-18 Helicos Biosciences Corporation Surface-capture of target nucleic acids
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9365897B2 (en) * 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
US9873907B2 (en) * 2013-05-29 2018-01-23 Agilent Technologies, Inc. Method for fragmenting genomic DNA using CAS9
EP4170044B1 (en) * 2014-06-06 2026-03-11 Cornell University Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions
HRP20200529T1 (hr) * 2014-06-06 2020-09-04 Regeneron Pharmaceuticals, Inc. Postupci i pripravci za modificiranje ciljnog lokusa
WO2016109255A1 (en) * 2014-12-30 2016-07-07 University Of South Florida Methods and compositions for cloning into large vectors
GB2565461B (en) * 2016-03-31 2022-04-13 Harvard College Methods and compositions for the single tube preparation of sequencing libraries using Cas9
GB201616590D0 (en) * 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
KR20220038604A (ko) * 2019-05-30 2022-03-29 래피드 제노믹스 엘엘씨 표적 게놈 영역의 유연한 및 고처리량 시퀀싱

Also Published As

Publication number Publication date
BR112022001539A2 (pt) 2022-03-22
ES3051342T3 (en) 2025-12-26
CN114286861B (zh) 2024-12-06
EP3969581B1 (en) 2025-10-08
JP2022534625A (ja) 2022-08-02
WO2020232081A3 (en) 2020-12-24
AU2020275301A1 (en) 2022-01-20
EP3969581A2 (en) 2022-03-23
EP3969581A4 (en) 2023-06-14
WO2020232081A2 (en) 2020-11-19
US20220220542A1 (en) 2022-07-14
KR20220039653A (ko) 2022-03-29
CN114286861A (zh) 2022-04-05
JP7854386B2 (ja) 2026-05-01

Similar Documents

Publication Publication Date Title
AU2021282536B2 (en) Polynucleotide enrichment using CRISPR-Cas systems
US20220389408A1 (en) Methods and compositions for phased sequencing
JP4226476B2 (ja) 環状化可能プローブを用いた複数の標的配列の分析と検出
US20100273662A1 (en) Method of preparing libraries of template polynucleotides
AU2006295556A1 (en) High throughput screening of mutagenized populations
EP3356552A1 (en) High molecular weight dna sample tracking tags for next generation sequencing
EP3969581B1 (en) Capture and analysis of target genomic regions
CN107250381B (zh) Dna集合的归一化迭代条形码和测序
EP1960541A1 (en) Method for high throughput screening of transposon tagging populations and massive parallel sequence identification of insertion sites
JP7788280B2 (ja) 標的遺伝子領域の柔軟かつハイスループット配列決定
HK1236561A1 (en) Polynucleotide enrichment using crispr-cas systems
HK1236561B (en) Polynucleotide enrichment using crispr-cas systems

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20220815

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20240917

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20250116

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20250213

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 5TH ANNIV.) - STANDARD

Year of fee payment: 5

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250508

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250508

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20250626

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20250626

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20251029

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20260226

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20260303

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20260303

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20260303