JP7851025B2 - アフィニティークロマトグラフィーの改善されたプロセス - Google Patents

アフィニティークロマトグラフィーの改善されたプロセス

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Publication number
JP7851025B2
JP7851025B2 JP2022566393A JP2022566393A JP7851025B2 JP 7851025 B2 JP7851025 B2 JP 7851025B2 JP 2022566393 A JP2022566393 A JP 2022566393A JP 2022566393 A JP2022566393 A JP 2022566393A JP 7851025 B2 JP7851025 B2 JP 7851025B2
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JP
Japan
Prior art keywords
buffer
protein mixture
protein
approximately
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022566393A
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English (en)
Japanese (ja)
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JP2023523824A (ja
JP2023523824A5 (https=
Inventor
ナラヤン，オム
クマール グプタ，タルン
タッカー，マヤンククマール
Original Assignee
カシーヴ バイオサイエンシズ，リミテッド・ライアビリティ・カンパニー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by カシーヴ バイオサイエンシズ，リミテッド・ライアビリティ・カンパニー filed Critical カシーヴ バイオサイエンシズ，リミテッド・ライアビリティ・カンパニー
Publication of JP2023523824A publication Critical patent/JP2023523824A/ja
Publication of JP2023523824A5 publication Critical patent/JP2023523824A5/ja
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4283—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
    • C07K16/4291—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig against IgE
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
JP2022566393A 2020-05-01 2021-05-01 アフィニティークロマトグラフィーの改善されたプロセス Active JP7851025B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN202021018721 2020-05-01
IN202021018721 2020-05-01
PCT/IB2021/053660 WO2021220253A1 (en) 2020-05-01 2021-05-01 An improved process of affinity chromatography

Publications (3)

Publication Number Publication Date
JP2023523824A JP2023523824A (ja) 2023-06-07
JP2023523824A5 JP2023523824A5 (https=) 2024-05-09
JP7851025B2 true JP7851025B2 (ja) 2026-04-24

Family

ID=78373380

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022566393A Active JP7851025B2 (ja) 2020-05-01 2021-05-01 アフィニティークロマトグラフィーの改善されたプロセス

Country Status (6)

Country Link
US (2) US20230166201A1 (https=)
EP (1) EP4142794A4 (https=)
JP (1) JP7851025B2 (https=)
AU (1) AU2021265346A1 (https=)
CA (1) CA3182315A1 (https=)
WO (1) WO2021220253A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2021262609A1 (en) 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140010820A1 (en) 2012-05-21 2014-01-09 Abbvie, Inc. Novel purification of non-human antibodies using protein a affinity chromatography

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080025174A (ko) * 2005-06-23 2008-03-19 메디뮨 인코포레이티드 응집 및 단편화 프로파일이 최적화된 항체 제제
CN101511387A (zh) * 2006-09-08 2009-08-19 惠氏公司 使用亲和色谱法纯化蛋白质的精氨酸洗涤液
WO2009058769A1 (en) * 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
EP2519536A4 (en) * 2009-12-29 2013-06-05 Reddys Lab Ltd Dr PROTEIN PURIFICATION BY ION EXCHANGE
AR089434A1 (es) * 2011-12-23 2014-08-20 Genentech Inc Procedimiento para preparar formulaciones con alta concentracion de proteinas
WO2013158279A1 (en) * 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
SG11201407809QA (en) * 2012-05-31 2014-12-30 Agency Science Tech & Res Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities
US20150284673A1 (en) * 2012-12-13 2015-10-08 Sapphire Energy, Inc. Harvesting algae from water
EP4549463A3 (en) * 2016-06-17 2025-07-30 F. Hoffmann-La Roche AG Purification of multispecific antibodies
CA3051466A1 (en) * 2017-01-31 2018-08-09 Vanderbilt University Generation of human allergen-and helminth-specific ige monoclonal antibodies for diagnostic and therapeutic use
US11408840B2 (en) * 2018-06-28 2022-08-09 Just-Evotec Biologics, Inc. High throughput interrogation of physiochemical properties of a protein

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140010820A1 (en) 2012-05-21 2014-01-09 Abbvie, Inc. Novel purification of non-human antibodies using protein a affinity chromatography

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Jin, W. et al.，"Protein aggregation and mitigation strategy in low pH viral inactivation for monoclonal antibody purification"，MAbs，2019年，Vol. 11，pp. 1479-1491
Skamris, T. et al.，"Monoclonal Antibodies Follow Distinct Aggregation Pathways During Production-Relevant Acidic Incubation and Neutralization"，Pharm. Res.，2016年，Vol. 33，pp. 716-728

Also Published As

Publication number Publication date
US20230166201A1 (en) 2023-06-01
AU2021265346A1 (en) 2022-12-22
EP4142794A4 (en) 2024-10-30
US20250099873A1 (en) 2025-03-27
AU2021265346A9 (en) 2023-04-13
CA3182315A1 (en) 2021-11-04
EP4142794A1 (en) 2023-03-08
WO2021220253A1 (en) 2021-11-04
JP2023523824A (ja) 2023-06-07

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