AU2021265346A1 - An improved process of affinity chromatography - Google Patents

An improved process of affinity chromatography Download PDF

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Publication number
AU2021265346A1
AU2021265346A1 AU2021265346A AU2021265346A AU2021265346A1 AU 2021265346 A1 AU2021265346 A1 AU 2021265346A1 AU 2021265346 A AU2021265346 A AU 2021265346A AU 2021265346 A AU2021265346 A AU 2021265346A AU 2021265346 A1 AU2021265346 A1 AU 2021265346A1
Authority
AU
Australia
Prior art keywords
protein mixture
buffer
protein
turbidity
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU2021265346A
Other languages
English (en)
Other versions
AU2021265346A9 (en
Inventor
Tarun Kumar Gupta
Om NARAYAN
Mayankkumar THAKKAR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kashiv Biosciences LLC
Original Assignee
Kashiv Biosciences LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kashiv Biosciences LLC filed Critical Kashiv Biosciences LLC
Publication of AU2021265346A1 publication Critical patent/AU2021265346A1/en
Publication of AU2021265346A9 publication Critical patent/AU2021265346A9/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4283—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
    • C07K16/4291—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig against IgE
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
AU2021265346A 2020-05-01 2021-05-01 An improved process of affinity chromatography Pending AU2021265346A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN202021018721 2020-05-01
IN202021018721 2020-05-01
PCT/IB2021/053660 WO2021220253A1 (en) 2020-05-01 2021-05-01 An improved process of affinity chromatography

Publications (2)

Publication Number Publication Date
AU2021265346A1 true AU2021265346A1 (en) 2022-12-22
AU2021265346A9 AU2021265346A9 (en) 2023-04-13

Family

ID=78373380

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2021265346A Pending AU2021265346A1 (en) 2020-05-01 2021-05-01 An improved process of affinity chromatography

Country Status (6)

Country Link
US (2) US20230166201A1 (https=)
EP (1) EP4142794A4 (https=)
JP (1) JP7851025B2 (https=)
AU (1) AU2021265346A1 (https=)
CA (1) CA3182315A1 (https=)
WO (1) WO2021220253A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2021262609A1 (en) 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080025174A (ko) * 2005-06-23 2008-03-19 메디뮨 인코포레이티드 응집 및 단편화 프로파일이 최적화된 항체 제제
CN101511387A (zh) * 2006-09-08 2009-08-19 惠氏公司 使用亲和色谱法纯化蛋白质的精氨酸洗涤液
WO2009058769A1 (en) * 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
EP2519536A4 (en) * 2009-12-29 2013-06-05 Reddys Lab Ltd Dr PROTEIN PURIFICATION BY ION EXCHANGE
AR089434A1 (es) * 2011-12-23 2014-08-20 Genentech Inc Procedimiento para preparar formulaciones con alta concentracion de proteinas
WO2013158279A1 (en) * 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
TW201348247A (zh) * 2012-05-21 2013-12-01 Abbvie Inc 利用蛋白質ａ親和性層析之非人類抗體之新穎純化
SG11201407809QA (en) * 2012-05-31 2014-12-30 Agency Science Tech & Res Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities
US20150284673A1 (en) * 2012-12-13 2015-10-08 Sapphire Energy, Inc. Harvesting algae from water
EP4549463A3 (en) * 2016-06-17 2025-07-30 F. Hoffmann-La Roche AG Purification of multispecific antibodies
CA3051466A1 (en) * 2017-01-31 2018-08-09 Vanderbilt University Generation of human allergen-and helminth-specific ige monoclonal antibodies for diagnostic and therapeutic use
US11408840B2 (en) * 2018-06-28 2022-08-09 Just-Evotec Biologics, Inc. High throughput interrogation of physiochemical properties of a protein

Also Published As

Publication number Publication date
US20230166201A1 (en) 2023-06-01
EP4142794A4 (en) 2024-10-30
US20250099873A1 (en) 2025-03-27
JP7851025B2 (ja) 2026-04-24
AU2021265346A9 (en) 2023-04-13
CA3182315A1 (en) 2021-11-04
EP4142794A1 (en) 2023-03-08
WO2021220253A1 (en) 2021-11-04
JP2023523824A (ja) 2023-06-07

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