JP7847714B2 - オリゴｄｎａの連結反応と連結オリゴｄｎａによるｄｎａ増幅の連続反応に基づく新規ライブラリ調製法 - Google Patents

オリゴｄｎａの連結反応と連結オリゴｄｎａによるｄｎａ増幅の連続反応に基づく新規ライブラリ調製法

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JP7847714B2
JP7847714B2 JP2025506903A JP2025506903A JP7847714B2 JP 7847714 B2 JP7847714 B2 JP 7847714B2 JP 2025506903 A JP2025506903 A JP 2025506903A JP 2025506903 A JP2025506903 A JP 2025506903A JP 7847714 B2 JP7847714 B2 JP 7847714B2
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reaction
sequence
oligo
target product
dna
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JPWO2024190829A1 (https=
JPWO2024190829A5 (https=
Inventor
信也 蔵田
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Nippon Steel Eco Tech Corp
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Nippon Steel Eco Tech Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2025506903A 2023-03-15 2024-03-13 オリゴｄｎａの連結反応と連結オリゴｄｎａによるｄｎａ増幅の連続反応に基づく新規ライブラリ調製法 Active JP7847714B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2023041409 2023-03-15
JP2023041409 2023-03-15
PCT/JP2024/009803 WO2024190829A1 (ja) 2023-03-15 2024-03-13 オリゴｄｎａの連結反応と連結オリゴｄｎａによるｄｎａ増幅の連続反応に基づく新規ライブラリ調製法

Publications (3)

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JPWO2024190829A1 JPWO2024190829A1 (https=) 2024-09-19
JPWO2024190829A5 JPWO2024190829A5 (https=) 2025-09-10
JP7847714B2 true JP7847714B2 (ja) 2026-04-17

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JP (1) JP7847714B2 (https=)
WO (1) WO2024190829A1 (https=)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017192349A (ja) 2016-04-21 2017-10-26 日鉄住金環境株式会社 多項目増幅手法
US20210115435A1 (en) 2017-04-06 2021-04-22 Qing Wang Error-proof nucleic acid library construction method
JP2022170730A (ja) 2021-04-28 2022-11-10 日鉄環境株式会社 ５’→３’エキソヌクレアーゼ活性等を有するｄｎａポリメラーゼを使用した遺伝子ライブラリーの新規調製方法
JP2022170729A (ja) 2021-04-28 2022-11-10 日鉄環境株式会社 遺伝子ライブラリーの新規調製方法
JP2023546330A (ja) 2020-09-22 2023-11-02 カタログ テクノロジーズ， インコーポレイテッド 温度制御流体反応システム

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017192349A (ja) 2016-04-21 2017-10-26 日鉄住金環境株式会社 多項目増幅手法
US20210115435A1 (en) 2017-04-06 2021-04-22 Qing Wang Error-proof nucleic acid library construction method
JP2023546330A (ja) 2020-09-22 2023-11-02 カタログ テクノロジーズ， インコーポレイテッド 温度制御流体反応システム
JP2022170730A (ja) 2021-04-28 2022-11-10 日鉄環境株式会社 ５’→３’エキソヌクレアーゼ活性等を有するｄｎａポリメラーゼを使用した遺伝子ライブラリーの新規調製方法
JP2022170729A (ja) 2021-04-28 2022-11-10 日鉄環境株式会社 遺伝子ライブラリーの新規調製方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN, Yongmei, et al.，Barcoded sequencing workflow for high throughput digitization of hybridoma antibody variable domain，Journal of Immunological Methods，2018年，vol. 455，pp. 88-94
MIYATA, Masashi, et al.，Compound-eye metasurface optics enabling a high-sensitivity, ultra-thin polarization camera，Optics Express，2020年03月23日，Vol. 28, No. 7，p.9996，DOI: 10.1364/OE.389591
高津京子、蔵田信也，新規一塩基多型解析技術の開発，Bio Industry，2005年，Vol. 22，pp. 63-69

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WO2024190829A1 (ja) 2024-09-19

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