JP7827410B2 - 改変糸状菌、及びそれを用いたタンパク質の製造方法 - Google Patents

改変糸状菌、及びそれを用いたタンパク質の製造方法

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Publication number
JP7827410B2
JP7827410B2 JP2021066415A JP2021066415A JP7827410B2 JP 7827410 B2 JP7827410 B2 JP 7827410B2 JP 2021066415 A JP2021066415 A JP 2021066415A JP 2021066415 A JP2021066415 A JP 2021066415A JP 7827410 B2 JP7827410 B2 JP 7827410B2
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Japan
Prior art keywords
ace3
seq
variant
amino acids
filamentous fungus
Prior art date
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JP2021066415A
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English (en)
Japanese (ja)
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JP2022161526A5 (enExample
JP2022161526A (ja
Inventor
俊陽 新井
裕司 児玉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kao Corp
Original Assignee
Kao Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority to JP2021066415A priority Critical patent/JP7827410B2/ja
Application filed by Kao Corp filed Critical Kao Corp
Priority to BR112023020870A priority patent/BR112023020870A2/pt
Priority to US18/286,138 priority patent/US20240182878A1/en
Priority to EP22784602.9A priority patent/EP4321608A4/en
Priority to CN202280027412.7A priority patent/CN117242168A/zh
Priority to PCT/JP2022/015855 priority patent/WO2022215618A1/ja
Publication of JP2022161526A publication Critical patent/JP2022161526A/ja
Publication of JP2022161526A5 publication Critical patent/JP2022161526A5/ja
Priority to JP2026027840A priority patent/JP2026076384A/ja
Application granted granted Critical
Publication of JP7827410B2 publication Critical patent/JP7827410B2/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01007—Acetylcholinesterase (3.1.1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021066415A 2021-04-09 2021-04-09 改変糸状菌、及びそれを用いたタンパク質の製造方法 Active JP7827410B2 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2021066415A JP7827410B2 (ja) 2021-04-09 2021-04-09 改変糸状菌、及びそれを用いたタンパク質の製造方法
US18/286,138 US20240182878A1 (en) 2021-04-09 2022-03-30 Modified filamentous fungus and method for producing protein using same
EP22784602.9A EP4321608A4 (en) 2021-04-09 2022-03-30 MUTANT FILAMENTOUS FUNGUS AND PROCESS FOR PRODUCTION OF PROTEIN USING IT
CN202280027412.7A CN117242168A (zh) 2021-04-09 2022-03-30 变异丝状菌和使用其的蛋白质的制造方法
BR112023020870A BR112023020870A2 (pt) 2021-04-09 2022-03-30 Fungo filamentoso modificado e método para produzir proteína usando o mesmo
PCT/JP2022/015855 WO2022215618A1 (ja) 2021-04-09 2022-03-30 改変糸状菌、及びそれを用いたタンパク質の製造方法
JP2026027840A JP2026076384A (ja) 2021-04-09 2026-02-24 改変糸状菌、及びそれを用いたタンパク質の製造方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2021066415A JP7827410B2 (ja) 2021-04-09 2021-04-09 改変糸状菌、及びそれを用いたタンパク質の製造方法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2026027840A Division JP2026076384A (ja) 2021-04-09 2026-02-24 改変糸状菌、及びそれを用いたタンパク質の製造方法

Publications (3)

Publication Number Publication Date
JP2022161526A JP2022161526A (ja) 2022-10-21
JP2022161526A5 JP2022161526A5 (enExample) 2024-04-10
JP7827410B2 true JP7827410B2 (ja) 2026-03-10

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021066415A Active JP7827410B2 (ja) 2021-04-09 2021-04-09 改変糸状菌、及びそれを用いたタンパク質の製造方法
JP2026027840A Pending JP2026076384A (ja) 2021-04-09 2026-02-24 改変糸状菌、及びそれを用いたタンパク質の製造方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2026027840A Pending JP2026076384A (ja) 2021-04-09 2026-02-24 改変糸状菌、及びそれを用いたタンパク質の製造方法

Country Status (6)

Country Link
US (1) US20240182878A1 (enExample)
EP (1) EP4321608A4 (enExample)
JP (2) JP7827410B2 (enExample)
CN (1) CN117242168A (enExample)
BR (1) BR112023020870A2 (enExample)
WO (1) WO2022215618A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021100631A1 (ja) 2019-11-18 2021-05-27 花王株式会社 変異糸状菌、及びそれを用いたタンパク質の製造方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130078674A1 (en) 2010-06-04 2013-03-28 Teknologian Tutkimuskeskus Vtt Method for protein production in filamentous fungi
JP2019528797A (ja) 2016-10-04 2019-10-17 ダニスコ・ユーエス・インク 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7713725B2 (en) 2002-09-10 2010-05-11 Danisco Us Inc. Induction of gene expression using a high concentration sugar mixture
FR2979111B1 (fr) 2011-08-19 2015-05-01 IFP Energies Nouvelles Procede de production de cellulases par un champignon filamenteux adapte a un fermenteur ayant un faible coefficient de transfert volumetrique d'oxygene kla
JP6114200B2 (ja) 2012-01-31 2017-04-12 国立大学法人長岡技術科学大学 セルロース系バイオマスからの糖及びアルコールの製造方法、並びに該方法に用いられる微生物

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130078674A1 (en) 2010-06-04 2013-03-28 Teknologian Tutkimuskeskus Vtt Method for protein production in filamentous fungi
JP2019528797A (ja) 2016-10-04 2019-10-17 ダニスコ・ユーエス・インク 誘導基質の非存在下における糸状菌細胞内でのタンパク質の産生

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Biotechnol. Biofuels，2014年，Vol.7，14 (pp.1-21)
Biotechnol. Biofuels，2020年，Vol.13，137 (pp.1-16)
Biotechnol. Biofuels，2020年，Vol.13，62 (pp.1-14)
J. Biol. Chem.，2019年，Vol.294, No.48，pp.18435-18450

Also Published As

Publication number Publication date
BR112023020870A2 (pt) 2023-12-12
US20240182878A1 (en) 2024-06-06
CN117242168A (zh) 2023-12-15
WO2022215618A1 (ja) 2022-10-13
EP4321608A1 (en) 2024-02-14
JP2026076384A (ja) 2026-05-11
JP2022161526A (ja) 2022-10-21
EP4321608A4 (en) 2025-12-03

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